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Citations for Bio-Rad :
1 - 50 of 619 citations for Saporin Chicken Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti–UNC-45 was affinity-purified using Affigel (BioRad)-conjugated to the MBP fusion ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and affinity purified using Affi-gel 10 (Bio-Rad, 153-6099). Commercial available antibodies are mouse anti-Flag (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Immunofluorescence labelling was performed to analyze the DC markers using FITC labeled mouse-anti-chicken MHC-II in combination with mouse anti-chicken CD40 (Bio-Rad, Hercules, CA), mouse anti-chicken CD11c (8F2 ...
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were purified by Nickel charged Immobilized Metal Affinity Chromatography (BioRad, CA). The affinity tags were removed during overnight incubation with SENP (made in-house) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The proteins were then purified by the kit of Ni-affinity purification (BioRad), followed by ammonium sulfate protein fractionation and size-exclusion chromatography on a Superdex Increase 200 10/300 GL column (Pharmacia Biotech ...
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bioRxiv - Microbiology 2022Quote: 6His-tagged proteins were affinity-purified using Gravity Flow Chromatography columns (Bio-Rad) loaded with 1-2 ml Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: Affinity purified proteins were separated by SDS-PAGE using Mini-PROTEAN TGX gels (BioRad) and stained with the Novex colloidal blue staining kit (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells and purified using immobilized metal affinity chromatography and a Profinia Purification System (BioRad) and size exclusion chromatography via an ÄKTA fast protein liquid chromatography system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... The labeled GyrA14-I491C was loaded onto a pre-equilibrated CcdB affinity column prepared using Affi-gel 10 (Bio-Rad), and incubated at 4°C for 4 hours on a rotary mixer to enable binding ...
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bioRxiv - Biophysics 2022Quote: ... His6-tagged proteins were purified using an Ni-NTA affinity column (Profinity IMAC Ni-Charged, BioRad).
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bioRxiv - Neuroscience 2022Quote: ... Antisera to GST-MTD was affinity purified by pre-absorption on an Affi-Gel (Bio-Rad) column coupled with GST ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were extracted and purified by immobilized metal affinity chromatography using Profinity IMAC resin (Bio-Rad) in a gravity flow column according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant proteins were affinity-purified from cleared lysates using a NGC Quest Plus FPLC system (Biorad) and GSTrap HP (GST-tagged BmVreteno and GST) ...
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bioRxiv - Cell Biology 2024Quote: ... The labeled antibodies are purified by P-6 Micro Bio-Spin Columns (BioRad).
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bioRxiv - Pathology 2019Quote: ... the cells were incubated with anti- MNV VP1 rabbit polyclonal antibody labeled by Dylight 488 (LNK221D488, BioRad) for 30 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: Deuterated LecB was purified by affinity chromatography using the NGC system (Bio-Rad, Marnes-la-Coquette, France) on a 10 mL mannose-Sepharose resin packed in the C10/10 column ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies against CBX2 were affinity purified using the Affi-gel (Bio-Rad, Affi-Gel 10, Cat# 1536099)-coupled FLAG-CBX2-ΔCBox ...
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bioRxiv - Physiology 2022Quote: ... and antibodies were affinity purified from the sera using Affigel 10 beads (BioRad Laboratories, Hercules, CA, USA) loaded with the antigens according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... The DMC1 antibody was then purified from antisera following affinity purification (using Affi-Gel 15 from Biorad) protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified over the corresponding GST-LRRCC1 fusion bound to Affi-Gel 10 resin (Bio-Rad). Other primary and secondary antibodies used in this study are listed in Table 1.
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bioRxiv - Biophysics 2021Quote: ... NRG1-Ig was purified under denaturing conditions via immobilized metal affinity chromatography (IMAC) using NGC system (Bio-Rad) equipped with a 10 mL Co-NTA column ...
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bioRxiv - Microbiology 2020Quote: ... MBP-MogR proteins were purified by amylose affinity chromatography (Poly-Prep Chromatography column, 0.8 × 4 cm, Bio-Rad). The identity and purity of proteins was confirmed by SDS-PAGE (Coomassie stain ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was purified from the soluble fraction by sequential affinity purification in gravity flow columns (Bio-Rad) using His60 Ni Superflow Resin (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Antibodies were purified from rabbit serum by affinity chromatography using Affi-Prep protein A resin cartridge (Bio-Rad).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Labeled probes were then purified using Micro Bio-Spin 6 Chromatography Column (BioRad, Berkeley, CA), and 1 μl of the probe was counted for subsequent radioactivity dilution calculations ...
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bioRxiv - Biochemistry 2021Quote: ... and the proteins were purified using a Profinia immobilized metal affinity chromatography (IMAC) column with Ni2+ beads (Bio-Rad) and a Profinia protein purification system (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Labeled antibodies were purified by size exclusion chromatography using Bio-Spin 6 columns (Bio-Rad, 7326200) followed by measurement of protein concertation using Nanodrop 2000 at 260 nm.
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bioRxiv - Biochemistry 2019Quote: ... Labeled proteins were purified by chloroform-methanol precipitation and concentration determined using the BCA assay (BioRad). Samples were digested over night at 37 °C with MS-grade trypsin (Serva, ...
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bioRxiv - Cancer Biology 2023Quote: ... Labeled antibodies were purified by size-exclusion chromatography using Bio-Spin 6 columns (Bio-Rad, 7326200) followed by measurement of protein concentration using a NanoDrop 2000 at 280 nm.
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bioRxiv - Neuroscience 2021Quote: ... the supernatant of the bacterial lysate was purified by affinity chromatography using an automated Profinia Protein Purification system (Bio-Rad), followed by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... and affinity purified anti-HSP-90 antibodies by use of an MBP-HSP-90 antigen coupled to Affigel matrix (BioRad), using a procedure previously published (Benian et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The BRM antigen was affinity-purified by IMAC using the NGC Medium-Pressure Liquid Chromatography System (Bio-Rad, Hercules, CA) and eluted in elution buffer (50mM sodium phosphate pH 8.0 ...
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bioRxiv - Synthetic Biology 2022Quote: We further purified both labeled and unlabeled miniproteins by size-exclusion chromatography (Bio-Rad NGC Chromatography System) using a Superdex 75 10/300 GL column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2020Quote: ... The labeled ASC-ST-DLL4 was then purified by desalting on a P-30 column (Bio-Rad). The final molar ratio of JF646 to ASC-ST-DLL4 was 5:1.
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bioRxiv - Biochemistry 2022Quote: ... The soluble recombinant protein within the supernatant was purified and dialysed using the Profinia Affinity Chromatography Protein Purification System (Bio-Rad), with the mini profinity IMAC and mini Bio-Gel P-6 desalting cartridges (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... 20 minutes) and the clear lysate was purified on HisTrap HP 1 ml affinity column (Cytiva) by FPLC system (NGC™, BioRad). The protein was eluted from the column using a linear gradient (Elution buffer ...
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bioRxiv - Biophysics 2023Quote: ... Solubilized protein was cleared by ultracentrifugation at 46,400 g and purified by metal affinity chromatography using a Nickel-charged EconoFit Nuvia IMAC column (Bio-Rad, USA). Protein was eluted in 20 mM HEPES ...
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bioRxiv - Genomics 2020Quote: ... Labeled probes were then purified using Micro Bio-Spin® Columns with Bio-Gel® P-30 (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was filtered through a 0.45-μm syringe filters and solubilized NFL was then purified from the supernatant by affinity chromatography using Bio-gel® HT hydroxyapatite (130-0150, Bio-Rad) and following the protocol of Leung and Liem [37] ...
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bioRxiv - Molecular Biology 2024Quote: Nuclear lysates of inputs and affinity-purified elution were loaded onto 4-15 % Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD, Cat# 5678085) and transferred to nitrocellulose membranes which were subsequently blocked by 5% BSA for 1Chour at RT ...
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bioRxiv - Developmental Biology 2019Quote: ... pH 8.0) prior to affinity purification by immobilized metal ion affinity chromatography (IMAC) using Chelex 100 Resin (Bio-Rad). Purified protein in PBS was mixed 1:1 with Freund’s complete adjuvant for immunization or with Freund’s incomplete adjuvant for booster injections ...
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bioRxiv - Cell Biology 2020Quote: ... TGN46 (sheep polyclonal, Bio-Rad), HA (mouse monoclonal ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... sheep polyclonal anti-TGN46 (AHP500G, Biorad), goat polyclonal anti-GM130 (P-20 ...
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bioRxiv - Cell Biology 2020Quote: ... TGN46-sheep polyclonal antibody (Bio-Rad) was used at 1:200 for immunofluorescence ...
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bioRxiv - Immunology 2020Quote: ... IgA-FITC polyclonal Ab (BioRad Antibodies) and IgM-PE mAb (clone K52 1C3 ...
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bioRxiv - Immunology 2023Quote: ... and C4d (Biorad 0300-0230, polyclonal). One sample from right ventricular endomyocardial biopsy was fixed in glutaralde-hyde and electron microscopy was performed (results not presented).
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bioRxiv - Plant Biology 2019Quote: ... HRP-labeled anti-rabbit antibody (Bio-Rad) was used as secondary antibody 1:10,000 in TBS-T containing 2 % low-fat milk powder for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were probed with appropriate secondary antibodies conjugated with Alexa488 fluorophore and imaged using the ChemiDoc system (Bio-Rad). The dot intensities were quantified with the Image Studio Lite software (LI-COR Biosciences ...