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Citations for Bio-Rad :
1 - 50 of 171 citations for SARS Coronavirus Spike Glycoprotein S1 His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal His tag antibody (MCA1396GA, Bio-Rad, 1:1000), mouse 2A antibody (NBP2-59627 ...
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bioRxiv - Microbiology 2021Quote: ... and analyzed by Western blot as previously described.21 Densitometry was performed to quantify the cleavage efficiency of full-length spike to S1/S2 subunits using ImageLab 6.0.1 (Bio-Rad #12012931). The average results of two experiments were presented.
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad). Copy number was calculated in reference to a standard curve of known copy number (102 – 107 copies spike) ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-SARS CoV-2 Spike Neutralizing antibody (SAD-S35, ACRO, USA) or anti-Human IgG Kappa (STAR 127, Bio-Rad, USA) antibody ...
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bioRxiv - Microbiology 2021Quote: ... were treated with 20% blocking buffer (Li-Cor Biosciences, Lincoln, US) followed by incubation with anti-His-tag mouse primary antibody (dilution1/5000, Bio-Rad) and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000 ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated with a secondary goat anti-rabbit (for the anti-YjbI antiserum) or goat anti–mouse (for the anti–His-tag antibody) antibody conjugated with horseradish peroxidase (Bio-Rad) for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... The 6× His-tag blots were washed with TMST and TSM and developed with the Clarity Western ECL kit (Bio-Rad) and imaged in a Gel-Doc system (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plaques of FCoV were stained with specific antibodies (primary antibody: mouse anti feline coronavirus monoclonal antibody at 1:500 dilution solution, Bio-Rad; secondary antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... Initial screening for IgG antibodies was undertaken using an extensive panel of bacterial (Leptospira, Brucella) and viral antigens (paramyxovirus, filovirus, coronavirus, flavivirus, alphavirus) coupled to MagPlex beads (Bio-Rad, Australia) for multiplex screening ...
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bioRxiv - Microbiology 2023Quote: ... slides were incubated for 30 min at room temperature with primary mouse monoclonal anti-feline coronavirus antibody at 1:400 (BioRad, MCA 2194). The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).
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bioRxiv - Microbiology 2022Quote: ... mouse-anti His (BIO-RAD; MCA1396GA), mouse-anti p65 (F-6 ...
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bioRxiv - Microbiology 2023Quote: ... A mouse anti-His antibody (Biorad) was used as a primary antibody in a 1:500 dilution ...
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bioRxiv - Microbiology 2021Quote: ... was used to detect and quantify SARS-CoV-2 RNA using the SARS-CoV-2 Droplet Digital PCR Kit (Bio-Rad), which contains a triplex assay of primers/probes aligned to the CDC markers for SARS-CoV-2 N1 and N2 genes and human RPP30 gene ...
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bioRxiv - Microbiology 2022Quote: ... Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10, Biorad) and anti-2A peptide (3H4 ...
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bioRxiv - Molecular Biology 2020Quote: Predesigned overlapping oligodeoxynucleotides (Fig S1) were assembled by PCR (BioRAD T100) using Primers F1 ...
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bioRxiv - Molecular Biology 2023Quote: ... a 160kD glycoprotein expressed by murine macrophages 235 (1:100, Bio-Rad clone C1:A3-1, Cat. MCA497) and rabbit anti-laminin (1:50 ...
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bioRxiv - Microbiology 2023Quote: gp120 or SOSIP glycoproteins were separated on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and transferred to a 0.45 μm nitrocellulose membrane (catalog number 1620115 ...
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bioRxiv - Neuroscience 2023Quote: ... Glycoproteins were eluted by boiling at 100°C for 10 minutes using 4x Laemmli sample buffer (Bio-Rad). Proteins were separated using a 4-12% Bis-Tris Protein SDS-PAGE Gel (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... Synthetic SARS-CoV-2 RNA was purchased from BIORAD (SARS-CoV-2 Standard #COV019 and SARS-CoV-2 Negative #COV000).
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bioRxiv - Cancer Biology 2021Quote: ... the anti-Strep-tag antibody from Biorad, the anti-rabbit IgG-HRP and anti-mouse IgG-HRP antibodies from GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: - Mouse anti-V5 tag (Bio-Rad, MCA1360) used at dilution 1:5000 for Western Blot
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bioRxiv - Genomics 2023Quote: - Mouse anti-V5 tag (Bio-Rad, MCA1360) used at dilution 1:5000 for Western Blot
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... Primer nucleotide sequences (Table S1) were determined using Beacon Designer software (Bio-Rad) and their specificity was analyzed by BLAST and PCR dissociation curve analysis ...
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bioRxiv - Biochemistry 2020Quote: ... anti-His5-Tag IgG-HRP (MCA5995P, Bio-Rad), anti-FLAG-Tag (M2 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-His5-Tag IgG-HRP (MCA5995P, Bio-Rad), anti-FLAG-Tag (M2 ...
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bioRxiv - Plant Biology 2020Quote: ... using gene specific primers (Table S1) and iTaq Universal SYBR Green Supermix (Bio-rad). The actin gene (OsAct1 ...
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bioRxiv - Plant Biology 2020Quote: ... 12.5 μL of SensiMix SYBR Hi-ROX (BIO-RAD) and DNAse/RNAse free water up to 15 μL ...
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bioRxiv - Microbiology 2023Quote: ... and an anti-His-HRP conjugate antibody (Bio-Rad) was used in a 1:4000 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse anti-V5 tag antibody (SV5-Pk1, Bio-Rad) and secondary antibodies (Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed using primers (Table S1) and Sso Advanced Universal SYBR Green Supermix (BioRad). Samples were processed as technical triplicates with the number of independent experimental replicates reported in the figure legends ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... lysates were incubated with 0.5μg V5-tag antibody (Bio-Rad) and ProteinG sepharose (GenScript ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using human-specific primers (Table S1) and iQ SYBR Green Supermix (Bio-Rad), with annealing temperature optimized by gradient PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected by electroporation in OPTI-MEM I using Gene-Pulser II (Bio-Rad) with 960 μF und 0.18 kV with 10 μg pcDNA3-FLAG-JUNB ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with mouse anti-His (1:1000, BioRad MCA1396GA) overnight at 4 °C and secondary antibody detection with IRDye800CW anti-mouse (1:5000 ...
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bioRxiv - Microbiology 2024Quote: ... PorH-His immunoblots were performed on 16.5% tris-tricine gels (BioRad) using anti-His (mouse ...
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... The protein eluates were adjusted to 250 mM NaCl and loaded onto a Uno S1 (Bio-rad) ion exchange column ...
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bioRxiv - Cell Biology 2021Quote: ... Target gene cDNA was amplified using primers (Table S1) and Sso Advanced Universal SYBR Green Supermix (BioRad) on a CFX384 Touch Real-Time PCR Detection system (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT PCR was performed with gene-specific primers (Table S1) using iTaq universal SYBR Green Supermix (BioRad) for 45 cycles on CFX96 Real time system (BioRad) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Mouse anti-V5-Tag antibody (MCA1360GA, Bio-Rad, Hercules, CA, USA) at 1:2000 dilution was used to detect V5-tagged v-Src ...
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bioRxiv - Plant Biology 2022Quote: ... Imaging was done using Hi-sensitivity chemiluminescence settings under ChemiDoc XRS+ (BioRad).
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bioRxiv - Neuroscience 2020Quote: ... The post-nuclear S1 fraction (supernatant) was collected and protein concentration measured using the BCA protein assay (Biorad).
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bioRxiv - Molecular Biology 2021Quote: Purified SARS-CoV-2 N protein was electrophoresed on 4-10% SDS-PAGE (Bio-Rad) and stained with Coomassie Brilliant Blue G-250 (CBBG-250) ...