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Citations for Bio-Rad :
1 - 50 of 138 citations for SARS Coronavirus Nucleoprotein NP His tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... or anti-Influenza A nucleoprotein (NP) mouse monoclonal (1:30) (MCA400, Bio-Rad). The next day ...
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bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal His tag antibody (MCA1396GA, Bio-Rad, 1:1000), mouse 2A antibody (NBP2-59627 ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and anti-canine distemper virus nucleoprotein mouse monoclonal primary antibody (Biorad) in selected fox tissues.
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bioRxiv - Microbiology 2021Quote: ... were treated with 20% blocking buffer (Li-Cor Biosciences, Lincoln, US) followed by incubation with anti-His-tag mouse primary antibody (dilution1/5000, Bio-Rad) and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000 ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated with a secondary goat anti-rabbit (for the anti-YjbI antiserum) or goat anti–mouse (for the anti–His-tag antibody) antibody conjugated with horseradish peroxidase (Bio-Rad) for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... The 6× His-tag blots were washed with TMST and TSM and developed with the Clarity Western ECL kit (Bio-Rad) and imaged in a Gel-Doc system (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... NP (BioRad Cat#MCA400, 1:1000), and anti-β-actin (Sigma Aldrich Cat #A2228-100UL ...
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bioRxiv - Immunology 2021Quote: ... and NP (BioRad Cat#MCA400, 1:1000) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The fixed and permeabilized cell monolayer was stained with anti-nucleoprotein (Clone: AA5H, Bio-Rad Antibodies, UK) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plaques of FCoV were stained with specific antibodies (primary antibody: mouse anti feline coronavirus monoclonal antibody at 1:500 dilution solution, Bio-Rad; secondary antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... Initial screening for IgG antibodies was undertaken using an extensive panel of bacterial (Leptospira, Brucella) and viral antigens (paramyxovirus, filovirus, coronavirus, flavivirus, alphavirus) coupled to MagPlex beads (Bio-Rad, Australia) for multiplex screening ...
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bioRxiv - Microbiology 2023Quote: ... slides were incubated for 30 min at room temperature with primary mouse monoclonal anti-feline coronavirus antibody at 1:400 (BioRad, MCA 2194). The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).
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bioRxiv - Microbiology 2022Quote: ... mouse-anti His (BIO-RAD; MCA1396GA), mouse-anti p65 (F-6 ...
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bioRxiv - Microbiology 2023Quote: ... A mouse anti-His antibody (Biorad) was used as a primary antibody in a 1:500 dilution ...
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bioRxiv - Microbiology 2021Quote: ... was used to detect and quantify SARS-CoV-2 RNA using the SARS-CoV-2 Droplet Digital PCR Kit (Bio-Rad), which contains a triplex assay of primers/probes aligned to the CDC markers for SARS-CoV-2 N1 and N2 genes and human RPP30 gene ...
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bioRxiv - Microbiology 2022Quote: ... Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10, Biorad) and anti-2A peptide (3H4 ...
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bioRxiv - Bioengineering 2021Quote: ... Synthetic SARS-CoV-2 RNA was purchased from BIORAD (SARS-CoV-2 Standard #COV019 and SARS-CoV-2 Negative #COV000).
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bioRxiv - Molecular Biology 2019Quote: ... 0.05% NP-40) and eluted with 2x Laemmli sample buffer (BioRad). Lysates and elutions were resolved by SDS-PAGE and western blotted in TBST using the following primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... the anti-Strep-tag antibody from Biorad, the anti-rabbit IgG-HRP and anti-mouse IgG-HRP antibodies from GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: - Mouse anti-V5 tag (Bio-Rad, MCA1360) used at dilution 1:5000 for Western Blot
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bioRxiv - Genomics 2023Quote: - Mouse anti-V5 tag (Bio-Rad, MCA1360) used at dilution 1:5000 for Western Blot
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... 0.05% NP-40) and eluted with 2× Laemmli sample buffer (Bio-Rad). 30 μg of whole-cell lysate or IP elutions were resolved on 4 to 15% mini-PROTEAN TGX gels (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... anti-His5-Tag IgG-HRP (MCA5995P, Bio-Rad), anti-FLAG-Tag (M2 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-His5-Tag IgG-HRP (MCA5995P, Bio-Rad), anti-FLAG-Tag (M2 ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.05% NP-40) and eluted by boiling in 2x Laemmli sample buffer (BioRad).
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bioRxiv - Plant Biology 2020Quote: ... 12.5 μL of SensiMix SYBR Hi-ROX (BIO-RAD) and DNAse/RNAse free water up to 15 μL ...
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bioRxiv - Microbiology 2023Quote: ... and an anti-His-HRP conjugate antibody (Bio-Rad) was used in a 1:4000 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse anti-V5 tag antibody (SV5-Pk1, Bio-Rad) and secondary antibodies (Goat anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2020Quote: ... arenaviral NP samples were separated on pre-cast 7.5% SDS-PAGE gels (Bio-Rad). The mitochondrial import assay results were analysed by visualizing the proteins with the Coomassie stain (PhastGel Blue R ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... lysates were incubated with 0.5μg V5-tag antibody (Bio-Rad) and ProteinG sepharose (GenScript ...
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bioRxiv - Microbiology 2021Quote: ... 30 μl of a primary antibody mix consisting of mouse anti-NP (Bio-Rad MCA400) and rabbit anti-JIP4 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected by electroporation in OPTI-MEM I using Gene-Pulser II (Bio-Rad) with 960 μF und 0.18 kV with 10 μg pcDNA3-FLAG-JUNB ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with mouse anti-His (1:1000, BioRad MCA1396GA) overnight at 4 °C and secondary antibody detection with IRDye800CW anti-mouse (1:5000 ...
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bioRxiv - Microbiology 2024Quote: ... PorH-His immunoblots were performed on 16.5% tris-tricine gels (BioRad) using anti-His (mouse ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Mouse anti-V5-Tag antibody (MCA1360GA, Bio-Rad, Hercules, CA, USA) at 1:2000 dilution was used to detect V5-tagged v-Src ...
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bioRxiv - Plant Biology 2022Quote: ... Imaging was done using Hi-sensitivity chemiluminescence settings under ChemiDoc XRS+ (BioRad).
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bioRxiv - Molecular Biology 2021Quote: Purified SARS-CoV-2 N protein was electrophoresed on 4-10% SDS-PAGE (Bio-Rad) and stained with Coomassie Brilliant Blue G-250 (CBBG-250) ...
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bioRxiv - Immunology 2023Quote: ... or sensitized with 1 mg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA), or in combination for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... 40 µg of sample protein was diluted with NP-40 buffer and SDS Sample Buffer (Bio-Rad) and were then loaded into 10% precast gels (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Western blots were performed using either mouse anti-V5 tag (Bio-Rad), 746-868 rabbit poly clonal IgG ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 specific genome levels were measured by qPCR using SsoFast EvaGreen supermix (Bio-Rad) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... 15 μg total RNA from HEK293 cells were separated on 5% Criterion™ TBE polyacrylamide gels (#3450048, Bio-Rad) and transferred to positively charged nylon membrane ...
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bioRxiv - Genetics 2021Quote: ... to detect endogenous RPA1 or anti-V5 tag antibodies (mouse, BioRad; 1:100) to detect ectopically expressed RPA1 ...
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bioRxiv - Neuroscience 2023Quote: ... SARS-CoV-2 RT-qPCR reactions (10 µL) using the iTaq Universal Probe One-Step Kit (BioRad) and SARS-CoV-2 N1 primers/probe mix (Stock# 10006713 ...
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bioRxiv - Microbiology 2023Quote: ... Grids were then blotted dry and suspended on a 25 µL drop of primary anti-NP antibody (Bio-Rad), diluted 1:500 in HN diluent ...