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Citations for Bio-Rad :
1 - 50 of 1177 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... was used to detect and quantify SARS-CoV-2 RNA using the SARS-CoV-2 Droplet Digital PCR Kit (Bio-Rad), which contains a triplex assay of primers/probes aligned to the CDC markers for SARS-CoV-2 N1 and N2 genes and human RPP30 gene ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad). Copy number was calculated in reference to a standard curve of known copy number (102 – 107 copies spike) ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Bioengineering 2021Quote: ... Synthetic SARS-CoV-2 RNA was purchased from BIORAD (SARS-CoV-2 Standard #COV019 and SARS-CoV-2 Negative #COV000).
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bioRxiv - Developmental Biology 2022Quote: ... anti-SARS CoV-2 Spike Neutralizing antibody (SAD-S35, ACRO, USA) or anti-Human IgG Kappa (STAR 127, Bio-Rad, USA) antibody ...
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).
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bioRxiv - Molecular Biology 2021Quote: Purified SARS-CoV-2 N protein was electrophoresed on 4-10% SDS-PAGE (Bio-Rad) and stained with Coomassie Brilliant Blue G-250 (CBBG-250) ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 specific genome levels were measured by qPCR using SsoFast EvaGreen supermix (Bio-Rad) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... SARS-CoV-2 RT-qPCR reactions (10 µL) using the iTaq Universal Probe One-Step Kit (BioRad) and SARS-CoV-2 N1 primers/probe mix (Stock# 10006713 ...
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bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 detection was performed on the CFX96™ Real-Time PCR Detection System (Bio-Rad, California, USA), using the NeoPlex™ COVID-19 Detection Kit (Genematrix ...
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bioRxiv - Molecular Biology 2022Quote: ... The SARS-COV-2 NTD expression was analysed with SDS-PAGE followed by Western-blot on PVDF membrane (Biorad) using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... Viral copy numbers were assessed via digital-droplet PCR (ddPCR) using SARS-CoV-2 ddPCR kit (#12013743) from Bio-Rad with N1 and N2 primers and probes targeting the nucleocapsid genes ...
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bioRxiv - Bioengineering 2022Quote: RNA was extracted as described above, followed by RT-PCR using the Allplex SARS-CoV-2 assay (SeeGene, South-Korea) on a CFX96 thermocycler (Biorad), simultaneously detecting four different target genes ...
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bioRxiv - Immunology 2023Quote: ... Bio-Plex SARS-CoV-2 Wuhan-1 strain RBD and N coupled beads (Bio-Rad, Cat Nos. 12015406 and 12014773), and Bio-Plex Pro Human IgA detection antibody (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... and by linear regression in log10 copies/ml with a standard curve realized from a SARS-CoV-2 positive nasopharyngeal sample quantified by Droplet-Digital PCR (Bio-Rad). IC50s were analyzed by nonlinear regression using a four-parameter dosage-response variable slope model with the GraphPad Prism 8.0.2 software (GraphPad Software ...
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bioRxiv - Microbiology 2021Quote: ... anti-FLAG (Cell Signalling Technologies, 2908S), anti-SARS-CoV-2-ORF8 (MRCPPU, University of Dundee, DA088, [37]) and anti-Tubulin (BioRad, MCA77G). Donkey anti-rabbit IRDye 800 and goat anti-rat IRDye 680 (Li-cor Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Optimized ddPCR was used to detect the presence of SARS-CoV-2 viruses following our previous study.10 Analysis of the ddPCR data was performed with QuantaSoft software (Bio-Rad). The concentration reported by the procedure equals copies of template per microliter of the final 1x ddPCR reaction ...
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bioRxiv - Immunology 2021Quote: ... and by linear regression in log10 copies/ml with a standard curve realized from a SARS-CoV-2 positive nasopharyngeal sample quantified by Droplet-Digital PCR (Bio-Rad). IC50s were analyzed by nonlinear regression using a four-parameter dosage-response variable slope model with the GraphPad Prism 8.0.2 software (GraphPad Software ...
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral copy numbers were determined using SARS-CoV-2 RdRp gene specific primers and probe (see [28]) and droplet digital PCR (Bio-Rad Laboratories Inc. ...
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bioRxiv - Genomics 2022Quote: ... Each sample was tested by PCR systems (threshold – 0,050) to the presence of SARS-CoV-2 using a CFX Connect Real-Time PCR System (BioRad, Hercules, California, USA). RT-PCR were implemented in 40 μl of final volumes ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd) ...
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bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Genetics 2023Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd.) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd.) ...
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bioRxiv - Immunology 2023Quote: Serum SARS-CoV-2-specific binding antibody assays were performed using the Bio-Plex Pro Human SARS-CoV-2 IgG 4-Plex Panel serology assay (Bio-Rad 12014634) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: 500 ng for each recombinant SARS-CoV-2 protein was prepared for electrophoresis using 4x Laemmli Sample Buffer (#1610747, Biorad Laboratories, Hercules, CA, USA) and incubated for 5 minutes at 95°C and then separated by 12% SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... in oral swabs of positive and negative subjects for SARS-CoV-2 using magnetic bead-based multiplex immunoassays (Bio-Plex Pro™ human cytokine 27-plex panel, Bio-Rad Laboratories, Milan, Italy) according to the pre-optimized protocol 15 ...
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bioRxiv - Microbiology 2021Quote: ... and analyzed by Western blot as previously described.21 Densitometry was performed to quantify the cleavage efficiency of full-length spike to S1/S2 subunits using ImageLab 6.0.1 (Bio-Rad #12012931). The average results of two experiments were presented.
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bioRxiv - Evolutionary Biology 2023Quote: ... and sheep polyclonal against Trans-Golgi network integral membrane protein 2 (TGN46; Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... TGN46 (sheep polyclonal, Bio-Rad), HA (mouse monoclonal ...
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bioRxiv - Cell Biology 2020Quote: ... and sheep anti-TGN46 (BioRad). Secondary Alexa Fluor-conjugated antibodies were from ThermoFisher and secondary peroxidase-conjugated antibodies were from Jackson ImmunoResearch ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... sheep polyclonal anti-TGN46 (AHP500G, Biorad), goat polyclonal anti-GM130 (P-20 ...
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bioRxiv - Cell Biology 2020Quote: ... TGN46-sheep polyclonal antibody (Bio-Rad) was used at 1:200 for immunofluorescence ...
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bioRxiv - Neuroscience 2023Quote: ... TTR (sheep, 1:100, Bio-rad), NEUROD2 (rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2% chelated FCS (Chelex 100 molecular biology grade resin, BioRad, USA), 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Sheep and-TGN46 (Biorad:AHP500G, IF-1:200); Mouse anti-Actin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... or HRP-rabbit anti- sheep secondary (BioRad) diluted 1:3,000 ...
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bioRxiv - Neuroscience 2021Quote: ... sheep anti-GFP 1:2000 (Bio-rad), chicken anti-PV 1:10000 (de Nooij et al. ...
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bioRxiv - Immunology 2020Quote: ... 5-μg/mL of sheep IgG (Biorad) and purified TNP (BD Pharmingen) ...
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bioRxiv - Molecular Biology 2020Quote: ... sheep polyclonal to TGN46 (Biorad #AHP500G, 1:1000), rat monoclonal to myc-tag (Chromotek #9e1 ...
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bioRxiv - Molecular Biology 2020Quote: ... or rabbit anti-sheep-HRP (Bio-Rad Laboratories) secondary antibodies (diluted 1:3000) ...
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bioRxiv - Cell Biology 2021Quote: sheep polyclonal anti-TGN46 (Biorad; 1:200, IFM); mouse monoclonal anti-β-actin [Sigma A5316 ...
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bioRxiv - Cell Biology 2019Quote: ... a sheep polyclonal antibody anti-TGN46 (BioRad, #AHP500), rabbit polyclonal antibodies anti-calnexin (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... or a sheep anti-GFP (BioRad, 4745-1051) and an antibody for microglia (anti-Iba1 ...
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bioRxiv - Cell Biology 2023Quote: ... Sheep anti-TGN38 is from Bio-Rad (#AHP499G). Mouse anti-α-actinin is from Sigma (#A7811) ...
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bioRxiv - Cell Biology 2023Quote: ... sheep anti-TGN46 (Bio-rad AHP500G, 1:1000), mouse anti-GM130 (BD 610822 ...