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Citations for Bio-Rad :
1 - 50 of 100 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... coli strain NEB10ß (Bio-Rad) carrying the BAC was electroporated with the pRed/ET expression plasmid using an Eppendorf Eporator® (Eppendorf ...
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bioRxiv - Molecular Biology 2023Quote: ... a 160kD glycoprotein expressed by murine macrophages 235 (1:100, Bio-Rad clone C1:A3-1, Cat. MCA497) and rabbit anti-laminin (1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... Glycoproteins were eluted by boiling at 100°C for 10 minutes using 4x Laemmli sample buffer (Bio-Rad). Proteins were separated using a 4-12% Bis-Tris Protein SDS-PAGE Gel (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: gp120 or SOSIP glycoproteins were separated on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and transferred to a 0.45 μm nitrocellulose membrane (catalog number 1620115 ...
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bioRxiv - Microbiology 2020Quote: ... prausnitzii strains using InstaGene™ Matrix (BioRad) kit according to the manufacturer guidelines ...
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bioRxiv - Developmental Biology 2020Quote: ... Plates were sealed with Microseal F (Biorad, MSF1001), centrifuged at 4ºC for 1min at 2000 rpm and stored at −80ºC.
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bioRxiv - Microbiology 2022Quote: ... falciparum Dd2 strain using a Gene Pulser Xcell (BioRad). Fifty micrograms of plasmid was mixed with 150 µl of packed parasitised-infected red blood cells and complete cytomix (120 mM KCl ...
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bioRxiv - Microbiology 2020Quote: ... and sealed with Microseal ‘F’ foil seals (Biorad, MSF1001). The duration of fixation was one-hour total ...
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bioRxiv - Microbiology 2023Quote: ... Goat F(ab)2 anti-human IgG: HRP (Biorad), Goat anti-mouse IgG (H/L) ...
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bioRxiv - Genomics 2021Quote: ... The strains were constructed by electroporation (Biorad MicroPulser program ShS) with a PCR-based gene targeting product leading to deletion of specific genes ...
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bioRxiv - Microbiology 2022Quote: ... the strains were subjected to disc diffusion tests (Bio-Rad) containing gentamicin (10 µg) ...
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bioRxiv - Molecular Biology 2022Quote: ... goat anti-human IgG F(ab’)2 (STAR126, Bio-Rad), anti-M13-pVIII-HRP (Cytiva 27-9421-01 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and SMD1163 strain using the electroporation method (GenePulser Xcell from Biorad). Transformants were selected ...
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bioRxiv - Microbiology 2023Quote: ... pneumoniae strains previously incubated in 25% of baby rabbit complement (BioRad) at 37°C for 30 mins were added to fresh human neutrophils extracted from blood from healthy volunteers using MACSxpress kit (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2020Quote: ... Plates were sealed immediately after sorting with microseal F foil (Biorad) and centrifuged at 4000g at 4°C for 5 min before being frozen on dry ice and stored at −80°C.
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bioRxiv - Immunology 2021Quote: ... The plates were sealed with a Microseal® ‘F’ Film (BioRad) and immediately frozen on dry ice before storage at −80°C for further use.
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bioRxiv - Immunology 2021Quote: ... First strain cDNA synthesis was performed using iScript cDNA synthesis kit (BioRad) and 8 μl of RNA ...
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bioRxiv - Microbiology 2021Quote: ... delemar pyrF mutant (strain M16)55 using the biolistic delivery system (BioRad), and the homogenous transformants were selected on minimal medium lacking uracil11 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was extracted from the strains using the InstaGenMatrix (Bio-Rad, USA) and stored at −20°C ...
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bioRxiv - Cell Biology 2023Quote: ... pastoris X33 strain using electroporation (MicroPulser Electroporator, Bio-Rad, Hercules, CA, USA). Recombinant expression of transformed yeast was induced by 1% methanol for 5 days ...
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bioRxiv - Biochemistry 2020Quote: ... with goat anti-human IgG F(ab’)2-HRP (STAR126P, Bio-Rad) as the detection antibody.
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bioRxiv - Biophysics 2021Quote: ... deglycosylation was performed using Remove-iT PNGase F (Bio-Rad, Hercules, CA). Samples with PNGase F were incubated at 310 K for 5 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit F(ab’)2 anti-mouse IgG conjugated to FITC (STAR9B, BioRad), and rabbit F(ab’)2 anti-rat IgG conjugated to FITC (STAR17B ...
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bioRxiv - Microbiology 2022Quote: ... The relative abundance of Spike transcripts was quantified by quantitative real-time PCR (qPCR) using CFX384 machine (BioRad) with a fluorescence reporter (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... aeruginosa strains were transformed by electroporation using a GenePulser II (BioRad, Hercules (CA), United States ...
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bioRxiv - Microbiology 2019Quote: ... Strains were grown in tryptocasein soy agar (TSA) (BioRad, Marnes-La-Coquette, France). The biochemical characterization of the isolates was performed using API20E strips (BioMérieux ...
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bioRxiv - Plant Biology 2022Quote: ... tumefaciens strain EHA101 (Hood et al., 1986) with a MicroPulser electroporator (Bio-Rad) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... paracasei BL23 strain by electroporation (25) with a Gene Pulser apparatus (Bio-Rad). Integrants at the ppx1 locus were selected on MRS plates supplemented with erythromycin ...
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bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
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bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
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bioRxiv - Cancer Biology 2021Quote: ... and rabbit F(ab’)2 anti-rat IgG conjugated to FITC (STAR17B, BioRad).
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bioRxiv - Cancer Biology 2021Quote: ... 1 μl of sequin spike (https://www.sequinstandards.com) was added to 14 μl of RNA for cDNA synthesis with SsoAdvanced iScript (Bio-Rad), followed by RT-qPCR using SsoAdvanced master mix (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates for quantitative Spike western blotting were loaded onto TGX Stain-Free 4-20% gradient gels (Bio-Rad, 4568094) (Fig ...
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bioRxiv - Cell Biology 2020Quote: ... All plasmids were transformed into BJ3505 wild-type and elo3Δ strains via electroporation (BIORAD) and plated onto appropriate auxotrophic selection plates.
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bioRxiv - Microbiology 2020Quote: ... coli strain S17-1 using a Gene Pulser Xcell™ (Bio-Rad, CA, USA) with the conditions of 2.5kV ...
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bioRxiv - Plant Biology 2021Quote: ... and transformed into Pichia pastoris yeast GS115 strain by electroporation using Micropulser Electroporator (BioRad). Then the GS115 cell was cultured on YPDS (the yeast extract peptone dextrose with sorbitol ...
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bioRxiv - Plant Biology 2021Quote: ... tumefaciens (strain C58C1) by electroporation with a MicroPulser electroporator (Bio-Rad, Hercules, CA, USA). A ...
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bioRxiv - Plant Biology 2024Quote: ... were separately introduced into Agrobacterium tumefaciens strain GV3101 using the MicroPulser electroporator (Bio-Rad). Agrobacterium was grown in liquid Luria-Bertani (LB ...
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bioRxiv - Genetics 2022Quote: ... Each plate was sealed using a Microseal “F” PCR plate seal (Bio-Rad, #MSF1001) and stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... and analyzed by Western blot as previously described.21 Densitometry was performed to quantify the cleavage efficiency of full-length spike to S1/S2 subunits using ImageLab 6.0.1 (Bio-Rad #12012931). The average results of two experiments were presented.
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bioRxiv - Immunology 2021Quote: ... Luminex beads were coupled to the Spike protein as previously described (63) and added to a Bio-Plex plate (BioRad). Beads were washed with PBS 0.05% tween using a magnetic plate washer (MAG2x program ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad). Copy number was calculated in reference to a standard curve of known copy number (102 – 107 copies spike) ...
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bioRxiv - Cancer Biology 2023Quote: ... KM12 or HCT116 cells (10 million) along with the spike-in control (20%) were carefully resuspended in cell suspension buffer (BioRad). The resultant homogeneous single-cell suspensions were embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit) ...
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bioRxiv - Plant Biology 2019Quote: ... rhizogenes strain K599 using the Bio-Rad Gene Pulser electroporation unit (Bio-Rad Laboratories, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... citri strain by electroporation (2.0 kV, 200 Ω, 25 µF, 0.2 cm cuvettes; Bio-Rad)(Sawitzke et al ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... All strains were transformed with the plasmid pSK265::DsRed by electroporation (Gene Pulser, Bio-Rad) and were grown at 37 °C on blood agar (43049 ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-SARS CoV-2 Spike Neutralizing antibody (SAD-S35, ACRO, USA) or anti-Human IgG Kappa (STAR 127, Bio-Rad, USA) antibody ...
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bioRxiv - Microbiology 2021Quote: ... coli strain S17-1 using a Gene Pulser Xcell™ (Bio-Rad, Hercules, CA, United States) with the conditions of 2.5kV ...
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bioRxiv - Genetics 2022Quote: An implant of each tested strain was placed on a cellophane layer (Biorad, Hercules, California, United States). After 2- ...
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bioRxiv - Plant Biology 2022Quote: ... The generated vectors were transformed into Agrobacterium tumefaciens strain GV3101 using a MicroPulser Electroporation system (Bio-Rad). After delivering the generated vectors to svr12 or atrmsD mutant plants using Agrobacterium-mediated transformation by the floral dip method (49) ...