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Citations for Bio-Rad :
1 - 50 of 8689 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Final recombinant protein concentrations were determined using Quick Start Bradford Protein Assays (Bio-Rad) with bovine serum albumin as the reference protein for standard curves ...
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bioRxiv - Immunology 2019Quote: Lung homogenates and a pre-stained recombinant protein ladder (Bio-Rad) were run in a 12% SDS-polyacrylamide gel and were transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Both sets of recombinant proteins were quantified using the Bradford protein quantification assay (Bio-Rad, UK).
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Immunology 2020Quote: ... These recombinant proteins were incubated with Affi-Prep Polymyxin Media (Bio-Rad) for 4 h at 4 °C to remove endotoxins and endotoxin-bound proteins ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins (10 μg) were applied to 4-20% gradient polyacrylamide gels (Bio-Rad) after heating them for 20 minutes at 95°C in 2x Laemmli buffer with 2% β-mercaptoethanol (BME) ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant protein was purified using the Ni-NTA (Biorad IMAC Ni-Charged Resins) affinity chromatography under denaturing conditions ...
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bioRxiv - Developmental Biology 2024Quote: The concentration of recombinant proteins was determined through a Bradford Assay (Bio-Rad, #5000006) using a NanoDrop One Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble recombinant protein within the supernatant was purified and dialysed using the Profinia Affinity Chromatography Protein Purification System (Bio-Rad), with the mini profinity IMAC and mini Bio-Gel P-6 desalting cartridges (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... CT analysis was performed using CFX Maestro software (BioRad).
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bioRxiv - Genomics 2020Quote: ... Ct analysis was performed using CFX Maestro software (BioRad). GAPDH was chosen as a reference gene because expression is relatively stable in SH-SY5Y cells79 ...
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bioRxiv - Biochemistry 2022Quote: ... Ct values were determined using CFX Maestro software (BioRad) and analyzed manually using the 2−ΔΔCT method.
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bioRxiv - Cell Biology 2022Quote: ... the cell lysates or recombinant protein were loaded on a 4-20% Criterion gel (BioRad, 3450032) for SDS-PAGE and transferred to PVDF membrane (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant proteins were affinity-purified from cleared lysates using a NGC Quest Plus FPLC system (Biorad) and GSTrap HP (GST-tagged BmVreteno and GST) ...
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bioRxiv - Genomics 2020Quote: ... The amplification Ct values were called automatically by Bio-Rad CFX Manager ...
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bioRxiv - Cancer Biology 2021Quote: ... Ct values were acquired with CFX Manager Software (Bio-Rad) and the relative chromosome copy numbers were calculated using a modified ΔΔCt method as described previously30.
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bioRxiv - Immunology 2022Quote: ... Threshold values (CT) were estimated using CFX Maestro 2.3 (Biorad) and transcript levels were normalized by subtracting the corresponding Gapdh values ...
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bioRxiv - Cell Biology 2023Quote: ... and Ct (cycle threshold) values were measured by Bio-Rad CFX96 Touch Real-Time PCR Detection System ...
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bioRxiv - Microbiology 2023Quote: ... The gel carrying the recombinant proteins and their derivatives was eventually stained with Coomassie Blue (Bio-Rad) for 1 hour and was de-stained with a de-staining solution (20% methanol and 10% acetic acid in water).
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein purities were assessed by SDS-PAGE using 4-20% Mini-Protean TGX gels (Bio-Rad, #4561094) and quantified using the BCA Protein Estimation kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... CT values were determined using the included CFX Maestro software (BioRad) or the QuantStudio 6 Flex ...
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bioRxiv - Microbiology 2023Quote: ... Ct values were obtained using the CFX Maestro Software (Bio-Rad) and fold-changes were calculated for each locus using the 2-ΔΔCt method and gyrB as the reference gene (60 ...
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bioRxiv - Cell Biology 2024Quote: ... and Ct values were determined using CFX Maestro 2.3 (Bio-Rad). Fold changes were calculated over the housekeeping gene and the corresponding control treatment using the 2^-ddCt method using Microsoft Excel ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Immunology 2020Quote: ... The Ct was determined by iQ5 software (Bio-Rad, Hercules, CA, USA) when the fluorescence rises exponentially 2-fold above background ...
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bioRxiv - Immunology 2020Quote: ... The Ct was determined by iQ5 software (Bio-Rad, Hercules, CA, USA) when the fluorescence rises exponentially 2-fold above background ...
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bioRxiv - Cancer Biology 2021Quote: ... Cycle threshold (Ct) values were calculated using CFX Maestro Software (Bio-Rad). Data analysis was completed using the GeneGlobe Data Analysis Center (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... The Ct was determined by iQ5 software (Bio-Rad, Hercules, CA, USA) when the fluorescence rises exponentially 2-fold above the background.
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bioRxiv - Genomics 2023Quote: ... Ct values were calculated using CFX Maestro software version 4.1.2433.1219 (Bio-Rad). Target gene expression was compared to reference gene expression using the 2-ΔΔCt method ...
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bioRxiv - Cancer Biology 2023Quote: ... Ct values were determined from the software CFX Maestro 1.1 (Bio-Rad), and the delta-delta Ct method was used to calculate the relative mRNA expressions of each target relative to the housekeeping gene(40).
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bioRxiv - Biophysics 2023Quote: ... Strep-tagged recombinant (BioRad, 1610363). The gel image was acquired with ChemiDoc Imaging Systems (BioRad ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were analysed by Western Blot using custom-made SLR1 specific polyclonal antibody with Mini-Protean II system (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Cell Biology 2023Quote: ... Ct values were obtained on CFX Opus 384 Real-Time PCR System (BioRad), and data was generated with the comparative threshold cycle (Delta CT ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2021Quote: ... and raw threshold-cycle time (Ct) values were calculated with iQ5 software (Bio-Rad). Mean values were calculated from samples and normalized to those obtained for Hprt transcripts as reported previously [Shi et al. ...
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bioRxiv - Genomics 2020Quote: ... The threshold cycle (Ct) was analyzed using BioRad CFX Manager 2.1 software (BioRad, USA).
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bioRxiv - Genetics 2021Quote: ... CT and melting curve results were calculated by the CFX Manager 3.1 software (BioRad). Results were normalized to β-actin or Gapdh using the ΔΔCt method [64].
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was determined by interpolation on to absorbance curves generated by recombinant protein standards using iMark™ Microplate Absorbance Reader (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Developmental Biology 2022Quote: ... and the delta-delta-ct method was used for quantification (CFX maestro software, Bio-Rad or QuantStudio 6 Flex Real-Time PCR System software ...
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bioRxiv - Immunology 2020Quote: ... The threshold cycle (Ct) values were determined by iQ5 software (Bio-Rad, Hercules, CA, USA). The fold change from the reference was calculated using the 2(Ct Sample—Housekeeping)/2(Ct Reference—Housekeeping ...
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bioRxiv - Genomics 2022Quote: ... The cycle threshold (CT) values were captured and calculated by the CFX Maestro (Bio-Rad) software and used as a measure of viral load.
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bioRxiv - Neuroscience 2024Quote: ... The resulting Ct (cycle threshold) values (obtained by the CFX ManagerTM Software, Bio-Rad Laboratories) were used to calculate relative expression levels for genes of interest by normalization to the housekeeping gene S12.
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bioRxiv - Bioengineering 2024Quote: ... Thermocycling and cycle threshold (ct) measurements was conducted on the CFX96™ qPCR instrument (Biorad). Gene expression levels were analyzed from ct values of the target genes and relative expression was quantified using the double-delta ct method.92 All primers used for qPCR are listed under Table S3.
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bioRxiv - Microbiology 2023Quote: ... 12 μl of purified recombinant orthologous proteins at a concentration of 250 nM were loaded onto a TGX FastCast 12% stain-free gel (Bio-Rad, Feldkirchen, Germany). Electrophoresis was conducted at 220 V for 30 min ...