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Citations for Bio-Rad :
1 - 50 of 1034 citations for Recombinant Mouse Tnf None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Strep-tagged recombinant (BioRad, 1610363). The gel image was acquired with ChemiDoc Imaging Systems (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... TNF-α) (BioRad). Data were analyzed using Bio-Plex Software Manager 6.1 ...
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bioRxiv - Immunology 2024Quote: ... TNF-α) were analyzed using Bio-Plex Pro Mouse Cytokine 8-plex Assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... TNF-α) were analyzed using Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2021Quote: ... using known concentrations of recombinant mouse IFN-γ (Bio-Rad, Oxford, UK) and a capturing of anti- IFN-γ mAb ...
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bioRxiv - Physiology 2023Quote: Human recombinant monoclonal antibodies to mouse ERFE were produced by Bio-Rad using HuCAL technology ...
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bioRxiv - Physiology 2023Quote: Human recombinant monoclonal antibodies to mouse ERFE were produced by Bio-rad using the HuCAL technology ...
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and TNF-α) from Bio-Rad (Catalog no. M60009RDPD). The manufacturer’s protocol was modified to accommodate a 384-well format by adding magnetic beads and antibodies at a reduced volume81,82 ...
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bioRxiv - Immunology 2021Quote: ... TNF-a and MCP-1 were purchased from Bio-Rad. For human cytokines ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and anti-mouse secondary antibody (Biorad; 1:10,000). GroEL was detected with anti-GroEL primary antibody (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated with anti-mouse or –rabbit secondary antibodies tagged with horseradish peroxidase (1/10 000, Bio-Rad Laboratories) for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-tagged secondary antibodies (Bio-Rad, 1721011 and 1721019) were used in combination with Super-Signal ECL kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Rhodamine-tagged anti-actin (Bio-Rad #12004163; 1:10,000) was used as a loading control ...
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bioRxiv - Microbiology 2023Quote: ... and TNF-α) were quantified via a 23-plex assay (Bio-Rad, Cat. No M60009RDP). Tissue samples were thawed on ice and then spun at 10,000g for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Neuroscience 2022Quote: ... and TNF was conducted on a Luminex Bio-Plex Platform (Bio-Rad Laboratories, Montreal, QC, Canada). Peripheral plasma cytokine levels were determined using Bio-Rad Pro mouse cytokine multiplex commercial kits (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... The β-tubulin antibody was tagged with Rhodamine (Bio-Rad #12004166) and was imaged using Rhodamine channel in Chemidoc-MP as per manufacturer’s instruction.
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged LC8 was purified using Ni Profinity IMAC Resin (BioRad) followed by anion-exchange chromatography on HiTrap Q-HP column (GE Healthcare) ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... The recombinant Human IgG1 Kappa (Biorad, cat.no. HCA192) was used as a positive control ...
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bioRxiv - Microbiology 2022Quote: 6His-tagged proteins were affinity-purified using Gravity Flow Chromatography columns (Bio-Rad) loaded with 1-2 ml Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... serial dilutions of recombinant NS1 Antigen (Ag) (Bio-Rad) was used ...
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bioRxiv - Microbiology 2020Quote: ... T7-tagged templates were generated with the iProof High-Fidelity PCR Kit (Bio-Rad) using sequences cloned into plasmids pIB (LacZ) ...
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bioRxiv - Neuroscience 2021Quote: ... and TNF-α were quantified using real-time PCR and the SsoAdvanced Universal SYBR Green Supermix Kit (BIO-RAD, Hercules, CA). β-actin was used as an internal control ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged fusion protein was purified using a Ni-NTA column (Bio-Rad, USA). Protein was eluted in elution buffer [500 mMNaCl ...
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bioRxiv - Microbiology 2024Quote: ... Detection of His-tagged proteins was performed with the ClarityTM Western ECL substrate (Biorad, USA) and using a Versadoc (Biorad ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant XRCC4-6xHis was immobilised on a GLC chip (BioRad Laboratories) in the vertical orientation ...
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bioRxiv - Immunology 2019Quote: Lung homogenates and a pre-stained recombinant protein ladder (Bio-Rad) were run in a 12% SDS-polyacrylamide gel and were transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Serially diluted recombinant human IgG1 or human IgG4 (BioRad, Hercules, CA) with unrelated specificities were used for quantification in separate wells on the same plate.
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bioRxiv - Immunology 2021Quote: ... TNF-α and VEGF-A using Luminex-based technology according to the manufacturer’s instructions on a Bio-Plex 200 (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2020Quote: ... TNF-β were detected by using the premixed 48-plex Bio-Plex Pro Human Cytokine Assay (Bio-Rad, Hercules, CA, USA; 12007283) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2022Quote: ... His6-tagged proteins were purified using an Ni-NTA affinity column (Profinity IMAC Ni-Charged, BioRad).
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bioRxiv - Immunology 2023Quote: ... Colorimetric (standards) and chemiluminescent detection (His-tagged proteins) were performed using the ChemiDoc Imager (Bio-Rad).
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bioRxiv - Microbiology 2019Quote: ... were coated with 1 μg/ml recombinant YFV NS1 (Biorad, cat # PIP052A) in 50 mM carbonate buffer (pH ...
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bioRxiv - Immunology 2020Quote: ... These recombinant proteins were incubated with Affi-Prep Polymyxin Media (Bio-Rad) for 4 h at 4 °C to remove endotoxins and endotoxin-bound proteins ...
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bioRxiv - Bioengineering 2020Quote: ... 0.4% β-mercaptoethanol) per lane or 1 μL of strep-tagged unstained protein standards (Bio-Rad # 1610363) and run at 100 V for 2 h in Tris-HCl-Glycine-SDS running buffer ...
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bioRxiv - Biophysics 2022Quote: ... His-tagged σs was added to phosphorylated/unphosphorylated RssB in Micro Bio-Spin Chromatography columns (Bio-Rad) before removing 6μL of the input for SDS-PAGE analysis and adding 40μL of prepared HisPur Ni-NTA resin (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... IL-6, TNF-α, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, IL-1RA, G-CSF and Eotaxin; Bio-Rad Laboratories Inc.) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... TNF-α) concentrations in blood plasma samples and tissue homogenate supernatants were determined with Bio-Plex Pro magnetic multiplex assays (Bio-Rad; Hercules, CA) and analyzed on the Bio-Plex 200 system with Bio-Plex Manager 5.0 software (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoblots were visualized using horseradish peroxidase–tagged secondary antibody and chemiluminescence (Clarity Western ECL Substrate; BioRad, Hercules, CA). The antibodies used included those directed to lamin A/C ...
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bioRxiv - Neuroscience 2021Quote: ... His6-tagged proteins were then captured from the lysate by Ni2+-IDA chromatography (Profinity IMAC resin, Bio-Rad) under gravity flow and washed with 35mM Imidazole ...
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bioRxiv - Cell Biology 2022Quote: Purified γ-TuRC preparations (GCP3- and GCP6-tagged) were resuspended in 20 μL of Laemmli Sample buffer (Biorad) and were loaded on a 4%–12% gradient Criterion XT Bis-Tris precast gel (Biorad) ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins (10 μg) were applied to 4-20% gradient polyacrylamide gels (Bio-Rad) after heating them for 20 minutes at 95°C in 2x Laemmli buffer with 2% β-mercaptoethanol (BME) ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...