Labshake search
Citations for Bio-Rad :
1 - 50 of 341 citations for Recombinant Human Insulin Receptor Isoform Short His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
-
bioRxiv - Biophysics 2023Quote: ... Strep-tagged recombinant (BioRad, 1610363). The gel image was acquired with ChemiDoc Imaging Systems (BioRad ...
-
bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
-
bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
-
bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2023Quote: ... Serially diluted recombinant human IgG1 or human IgG4 (BioRad, Hercules, CA) with unrelated specificities were used for quantification in separate wells on the same plate.
-
bioRxiv - Plant Biology 2020Quote: ... The His-tagged fusion protein was purified using a Ni-NTA column (Bio-Rad, USA). Protein was eluted in elution buffer [500 mMNaCl ...
-
bioRxiv - Microbiology 2024Quote: ... Detection of His-tagged proteins was performed with the ClarityTM Western ECL substrate (Biorad, USA) and using a Versadoc (Biorad ...
-
Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... The recombinant Human IgG1 Kappa (Biorad, cat.no. HCA192) was used as a positive control ...
-
bioRxiv - Immunology 2023Quote: ... Colorimetric (standards) and chemiluminescent detection (His-tagged proteins) were performed using the ChemiDoc Imager (Bio-Rad).
-
bioRxiv - Biophysics 2022Quote: ... His-tagged σs was added to phosphorylated/unphosphorylated RssB in Micro Bio-Spin Chromatography columns (Bio-Rad) before removing 6μL of the input for SDS-PAGE analysis and adding 40μL of prepared HisPur Ni-NTA resin (Thermo Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... and specific primers for ANKRD55 isoform 201 (Bio-Rad) were used to generate droplets in a QX200 Droplet Generator (Bio-Rad) ...
-
bioRxiv - Physiology 2023Quote: Human recombinant monoclonal antibodies to mouse ERFE were produced by Bio-Rad using HuCAL technology ...
-
bioRxiv - Physiology 2023Quote: Human recombinant monoclonal antibodies to mouse ERFE were produced by Bio-rad using the HuCAL technology ...
-
bioRxiv - Biochemistry 2021Quote: ... The recovery of tag-less MlaC was quantified using the ratio standard curve method (41) while that of His-tagged nanodisc-embedded complexes were quantified using the Lowry assay (DC Protein Assay, Bio-Rad). For retrograde assay ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then washed 3 times with 1X TBST for 10 minutes each before being transferred to a solution containing enhanced chemiluminescence (ECL) substrate to allow visualization of His-tagged sfGFP (Bio-Rad). The blotted proteins were visualized in a ChemiDoc Imaging System (Bio-Rad ...
-
bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
-
bioRxiv - Immunology 2021Quote: ... specific for the various human chemokine receptor genes and IQ™ SYBR green reagent (# 170882, BIO-RAD) on CFX96 real-time PCR detection system (BIO-RAD) ...
-
bioRxiv - Pathology 2019Quote: ... Affi-gel 10 affinity resins were covalently cross-linked to his-tagged GT198 protein as antigen for affinity purification of anti-GT198 according to the manufacturer’s protocol (Bio-Rad, Hercules, CA). FFPE tumor sections or tumor microarrays were deparaffinized and dehydrated through xylene and ethanol series ...
-
bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
-
bioRxiv - Synthetic Biology 2019Quote: ... In short: The CriterionTM system from BioRad (München, Germany) was used to run SDS-PAGE ...
-
bioRxiv - Bioengineering 2020Quote: ... anti-CD63 and anti-CD81 detection antibodies (supplied in the MACSPlex kit, 5 µl each) or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Short transfer using Trans-Blot Turbo transfer system (Bio-Rad) with the pre-set 10-minute-high MW program was used for all westerns unless blotting for full length Rif1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... mini-PROTEAN® short plates (Bio-Rad, Cat. No. 1653308), and 15-well 1.5mm mini-PROTEAN combs (Bio-Rad ...
-
bioRxiv - Immunology 2022Quote: TGF-beta isoforms were measured with the Bio-Plex Pro TGF-β assays (Bio-Rad) in accordance with manufactures directions ...
-
bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... To detect OPA1 isoforms proteins were resolved on 7.5% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Then proteins were transferred to a 0.2-μM PVDF membrane by Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Microbiology 2022Quote: ... mouse-anti His (BIO-RAD; MCA1396GA), mouse-anti p65 (F-6 ...
-
bioRxiv - Microbiology 2023Quote: ... A mouse anti-His antibody (Biorad) was used as a primary antibody in a 1:500 dilution ...
-
bioRxiv - Biophysics 2021Quote: The expression of the Triobp alternative splice isoforms was quantified using a QX200 ddPCR System (Bio-Rad, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... Ratios of active phosphorylated ROR2 : unphosphorylated ROR2 isoforms were calculated by quantitating band intensity using ImageLab 5.2.1 software (BioRad Inc.) for three biological replicates ...
-
bioRxiv - Immunology 2022Quote: ... The gel images were acquired using a ChemiDoc™ MP Imaging System and quantification of the pDNA isoform ratios were determined through densitometric analysis using the software ImageLab 6.0.1 (BioRad, USA).
-
bioRxiv - Cancer Biology 2019Quote: ... Insulin levels were measured on a BioPlex analyser (BioRad) using the Mouse Metabolic Magnetic bead panel kit (Reference ...
-
bioRxiv - Cell Biology 2022Quote: ... HRP-tagged secondary antibodies (Bio-Rad, 1721011 and 1721019) were used in combination with Super-Signal ECL kit (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2023Quote: ... Rhodamine-tagged anti-actin (Bio-Rad #12004163; 1:10,000) was used as a loading control ...
-
bioRxiv - Genetics 2022Quote: ... from individuals with DMBT1 size isoform I-IV phenotypes were separated by electrophoresis on 4-15% gradient SDS-PAGE gels (BioRad) and subjected to Western blot-like binding of biotinylated bacteria ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5 ng of cDNA was analyzed with isoform-specific droplet digital PCR assays and ddPCR Supermix for probes (Bio-Rad). Primers and probes (Integrated DNA Technologies ...
-
Negative curvature-promoting lipids instruct nuclear ingression of low autophagic potential vacuolesbioRxiv - Cell Biology 2021Quote: ... The same volume of supernatant was used to separate GFP from GFP-Atg8 isoforms onto a commercial 4–20% acrylamide gradient gel (BioRad) and migrated 45 min at 100 V in 1x MES buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... and guinea pig anti-insulin (BioRad 5330-0104G; 1:500). Highly cross adsorbed fluorescently conjugated secondary antibodies were used ...
-
bioRxiv - Plant Biology 2022Quote: ... A short electrophoresis run (5 minutes at 200 V) in TGS buffer (Bio-Rad, California, USA) was performed ...
-
bioRxiv - Cell Biology 2019Quote: ... estrogen receptor (Santa Cruz sc-542, 1:20; BioRad MCA1799T, 1:25), Ltf (Bioss bs-5810 ...
-
bioRxiv - Physiology 2024Quote: ... Fc-receptors were then blocked for 15 minutes using FcBlock (BioRad – BUF041A). Cells were pelleted by centrifugation (300 x g,5 minutes ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Fc-receptors were then blocked for 15 minutes using FcBlock (BioRad – BUF041A). Cells were pelleted by centrifugation (300 x g ...
-
bioRxiv - Plant Biology 2020Quote: ... 12.5 μL of SensiMix SYBR Hi-ROX (BIO-RAD) and DNAse/RNAse free water up to 15 μL ...
-
bioRxiv - Microbiology 2023Quote: ... and an anti-His-HRP conjugate antibody (Bio-Rad) was used in a 1:4000 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
-
bioRxiv - Microbiology 2019Quote: ... and cross-linked with short-wave UV light in a GS Gene Linker UV Chamber (Bio-Rad). A North2South Chemiluminescent Hybridization and Detection Kit (Thermo Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... and cross-linked with short-wave UV light in a GS Gene Linker UV Chamber (Bio-Rad). A North2South Chemiluminescent Hybridization and Detection Kit (Thermo Scientific ...