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Citations for Bio-Rad :
1 - 50 of 8685 citations for Recombinant E. coli MUG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Plasmids were transferred to C. sporogenes by conjugation as described before (Heap et al., 2009) using E. coli CA434 (E. coli HB101 (Bio-Rad Laboratories, The Netherlands) harboring the broad host IncPß+ conjugational plasmid pRK24 (Williams et al. ...
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bioRxiv - Microbiology 2023Quote: ... Final recombinant protein concentrations were determined using Quick Start Bradford Protein Assays (Bio-Rad) with bovine serum albumin as the reference protein for standard curves ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was detected using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Immunology 2019Quote: Lung homogenates and a pre-stained recombinant protein ladder (Bio-Rad) were run in a 12% SDS-polyacrylamide gel and were transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: Electroporations of A. baumannii were performed using 0.1cm gap cuvettes (0.2cm for E. coli) and the Gene Pulser (Bio-Rad) at the settings 200 Ω ...
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bioRxiv - Immunology 2023Quote: ... Both sets of recombinant proteins were quantified using the Bradford protein quantification assay (Bio-Rad, UK).
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Plant Biology 2022Quote: ... Finished CRISPR/Cas9 binary vectors were transformed into Agrobacterium tumefaciens EHA105 (Hood et al., 1993) by electroporation (E. coli Pulser, BioRad).
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bioRxiv - Cell Biology 2023Quote: ... Episome plasmids were transformed into E. coli (TransforMax EPI300) harboring the pTA_Mob51 mobility plasmid (gift from R. Lale) via electroporation (Bio-Rad). Transformed cells were spread onto LB agar plates containing both gentamycin (10 μg mL-1 ...
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bioRxiv - Immunology 2020Quote: ... These recombinant proteins were incubated with Affi-Prep Polymyxin Media (Bio-Rad) for 4 h at 4 °C to remove endotoxins and endotoxin-bound proteins ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins (10 μg) were applied to 4-20% gradient polyacrylamide gels (Bio-Rad) after heating them for 20 minutes at 95°C in 2x Laemmli buffer with 2% β-mercaptoethanol (BME) ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant protein was purified using the Ni-NTA (Biorad IMAC Ni-Charged Resins) affinity chromatography under denaturing conditions ...
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bioRxiv - Developmental Biology 2024Quote: The concentration of recombinant proteins was determined through a Bradford Assay (Bio-Rad, #5000006) using a NanoDrop One Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... The binary vector pMGE599 was introduced into Agrobacterium tumefaciens AGL-1 strain (62) through electroporation (E. coli Pulser; Bio-Rad, http://www.bio-rad.com). The scutella tissue of barley caryopsis was used for Agrobacterium-mediated transformation as described previously (63) ...
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bioRxiv - Biochemistry 2022Quote: ... blots were imaged using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Biochemistry 2022Quote: ... The soluble recombinant protein within the supernatant was purified and dialysed using the Profinia Affinity Chromatography Protein Purification System (Bio-Rad), with the mini profinity IMAC and mini Bio-Gel P-6 desalting cartridges (Bio-Rad) ...
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bioRxiv - Systems Biology 2020Quote: ... coli K12 whole cell lysates were analysed using 10% Mini-PROTEAN Protein Gels (4568034, Bio-Rad, Laemmli buffer S3401-VL ...
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bioRxiv - Cell Biology 2022Quote: ... the cell lysates or recombinant protein were loaded on a 4-20% Criterion gel (BioRad, 3450032) for SDS-PAGE and transferred to PVDF membrane (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant proteins were affinity-purified from cleared lysates using a NGC Quest Plus FPLC system (Biorad) and GSTrap HP (GST-tagged BmVreteno and GST) ...
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bioRxiv - Microbiology 2023Quote: ... The gel carrying the recombinant proteins and their derivatives was eventually stained with Coomassie Blue (Bio-Rad) for 1 hour and was de-stained with a de-staining solution (20% methanol and 10% acetic acid in water).
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bioRxiv - Microbiology 2021Quote: ... coli Pulser (Bio-Rad). Electroporation was performed under same conditions as previously described47 ...
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Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... coli antibodies (BioRad, ON) or rabbit anti-Salmonella O antiserum group B antibodies (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein purities were assessed by SDS-PAGE using 4-20% Mini-Protean TGX gels (Bio-Rad, #4561094) and quantified using the BCA Protein Estimation kit (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... coli HU protein was monitored using 20% SDS-PAGE and the concentration was determined using the Lorry assay (Biorad). The purified E ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Microbiology 2020Quote: ... coli strain NEB10ß (Bio-Rad) carrying the BAC was electroporated with the pRed/ET expression plasmid using an Eppendorf Eporator® (Eppendorf ...
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bioRxiv - Immunology 2024Quote: ... coli using the Micropulser (BioRad) with pulse setting Ec1 ...
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bioRxiv - Biophysics 2023Quote: ... Strep-tagged recombinant (BioRad, 1610363). The gel image was acquired with ChemiDoc Imaging Systems (BioRad ...
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bioRxiv - Immunology 2024Quote: ... 1:1200 diluted mouse anti-human HLA-E monoclonal primary antibody (clone MEM-E/02, Bio-Rad) and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were analysed by Western Blot using custom-made SLR1 specific polyclonal antibody with Mini-Protean II system (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... the soluble fraction containing recombinant MBP-RON11-His6 protein was affinity purified twice on immobilized-metal affinity chromatography (IMAC) column using FPLC (Biorad) and further purified on CHT Ceramic Hydroxyapatite column ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Genetics 2023Quote: ... The rabbit polyclonal antiserum was raised against purified recombinant Thp1 protein and subsequently affinity purified as Thp1p coated affinity beads (Affi-Gel 10 beads, Bio-Rad) according to standard procedures ...
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bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2021Quote: ... coli antibody (1:100, Bio-Rad), anti-LAMP1 (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... coli Pulser™ Transformation Apparatus (BioRad) was used to electroporate One Shot™ TOP10 Electrocomp™ E ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was determined by interpolation on to absorbance curves generated by recombinant protein standards using iMark™ Microplate Absorbance Reader (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Biophysics 2020Quote: ... coli RNAP (Bio-rad, Hercules, CA, USA) to a final concentration of 45.5 nM ...
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bioRxiv - Microbiology 2022Quote: ... coli Gene Pulser (Bio-Rad, Hercules, CA). All pDN201-derived plasmids were verified by Sanger sequencing at the UC Berkeley DNA Sequencing Facility and all pAMG40 cointegrates were verified by restriction endonuclease analysis.
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bioRxiv - Microbiology 2023Quote: ... coli settings (1.8 kV, Bio-Rad MicroPulser). With a 1000 µL pipette ...
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bioRxiv - Microbiology 2023Quote: ... 12 μl of purified recombinant orthologous proteins at a concentration of 250 nM were loaded onto a TGX FastCast 12% stain-free gel (Bio-Rad, Feldkirchen, Germany). Electrophoresis was conducted at 220 V for 30 min ...
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bioRxiv - Biochemistry 2021Quote: 500 ng for each recombinant SARS-CoV-2 protein was prepared for electrophoresis using 4x Laemmli Sample Buffer (#1610747, Biorad Laboratories, Hercules, CA, USA) and incubated for 5 minutes at 95°C and then separated by 12% SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: The recombinant protein of each vaccine candidate was purified under denaturing conditions using a biologic duoflow™ chromatography system (Bio-Rad Laboratories, Hercules, USA). A HisTrap™ HP column (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... coli Dh5α using IProof DNA polymerase from BioRad and restriction enzymes from New English Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... coli Gene Pulser® (Biorad, Hercules, CA, USA), as recommended by the supplier ...
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bioRxiv - Biophysics 2023Quote: ... coli was purchased from Bio-Rad (Hercules, CA) and received as a lyophilized powder ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... The recombinant Human IgG1 Kappa (Biorad, cat.no. HCA192) was used as a positive control ...