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Citations for Bio-Rad :
1 - 19 of 19 citations for Protease since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 60 units of Prescision protease (BioRad Laboratories) were added to the beads and cleavage of the GST-Tag was allowed for 1 h at room temperature followed by an overnight incubation at 4 C on a rotating wheel ...
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bioRxiv - Cancer Biology 2019Quote: ... lysis buffer supplemented with protease inhibitor cocktail (Pierce) and quantified (BioRad DC protein assay). 30 μg of lysate was loaded to SDS-polyacrylamide gels ...
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bioRxiv - Molecular Biology 2021Quote: ... the eggs were sonicated in RIPA buffer supplemented with a protease inhibitor (Bio-Rad Laboratories, Inc.) in the proportion of 25 mg of eggs per 100 microlitres of buffer ...
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bioRxiv - Biophysics 2021Quote: ... and protease inhibitors and the protein content quantified using a detergent-compatible protein assay (Bio-Rad). Lysates (10 μg ...
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bioRxiv - Plant Biology 2023Quote: ... 0.3% (w/v) CHAPS containing protease inhibitors (1 tablet Biorad/10ml ; 1/100 v/v PMSF). Phosphatase inhibitors were also added in L3 and L4 experiments ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.4 and protease inhibitors) and quantified using the BCA protein determination method (Bio-Rad, Hercules, CA). Protein samples were diluted to equivalent volumes containing 20 μg of protein and boiled in an equal volume of Laemli SDS boiling buffer (Sigma ...
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bioRxiv - Immunology 2022Quote: ... supplemented with Protease and Phosphatase Inhibitor and loaded on 4–15% SDS-PAGE gels (Bio-Rad Laboratories, Inc.). Proteins were transferred on a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2021Quote: ... protease inhibitor cocktail) and protein concentrations were determined by Bradford protein assay (Bio-Rad Laboratories, Inc. Hercules, CA, USA) and absorbance was measured at 595 nm on the Tecan Infinite® 2000 PRO plate reader (Tecan ...
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bioRxiv - Molecular Biology 2021Quote: ... for 24 hours before being lysed in RIPA buffer containing protease inhibitors and protein concentration assessed using RcDc assay (BioRad). In some experiments cells were treated with 2μM of VX-445 (Selleckchem) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were diluted 1:10 in RIPA Lysis and Extraction buffer (Thermo Fischer) supplemented with protease inhibitor cocktail (Fischer Scientific) for use in BCA protein assay (BioRad). BCA protein assay was performed in triplicate for each diluted sample following manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Lungs were homogenized in D-PBS containing protease inhibitors to perform the cytokines quantification using a commercial multiplex mouse cytokine bead assay (Bio-Rad, Bio-Plex Pro Mouse Cytokine 23-plex Assay ...
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bioRxiv - Neuroscience 2022Quote: E15.5 forebrains were dissected/frozen on dry ice and then lysed in standard RIPA buffer with protease and phosphatase inhibitors and combined with Laemmli buffer (BioRad 1610737EDU) containing 2-Mercaptoethanol and incubated at 95°C for 5 minutes ...
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bioRxiv - Biophysics 2022Quote: ... Resulting membranes were resuspended in 100 μL solubilization buffer (1xPBS, 1% DDM, EDTA-free protease inhibitors) and total protein concentration was quantified using DC protein assay (Bio-Rad). For each condition ...
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bioRxiv - Microbiology 2023Quote: ... brucei PFs were prepared in 1% Triton X-100 in PBS with 1X protease inhibitor cocktail (Bio-Vision) and mixed in 4X Laemmli buffer (Bio-Rad) with 710 mM β-mercaptoethanol and heated for 5 min at 95°C ...
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bioRxiv - Developmental Biology 2024Quote: ... discs from third-instar larvae were dissected in Ringer’s Solution supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail on ice and then lysed in 2x Laemmli Sample Buffer (Biorad #1610737EDU) and heat denatured at 95°C for 5 minutes.
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bioRxiv - Physiology 2024Quote: ... Pierce™ Protease& Phosphatase Inhibitor Mini Tablets) and final protein concentration was determined with BCA protein Assay (Biorad Laboratories, Feldkirchen, Germany). Heart tissue samples of the infarct areas were excised (2 weeks or 8 weeks post-surgery ...
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bioRxiv - Bioengineering 2020Quote: Mouse tissues from mdx4cv mice treated with or without AAV9-NG or AAV9-iNG were lysed with cold RIPA buffer supplemented with protease inhibitors and extracted protein samples were separated by SDS-PAGE (BioRad, 4-15%) and transferred onto Nitrocelluloase membranes (0.45 μm) ...
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bioRxiv - Physiology 2019Quote: ... 1 cOmplete™ protease inhibitor cocktail EDTA free tablet) and protein concentrations determined using the DC protein assay (Bio-Rad, Hercules, CA). Oxygen Consumption Rate (OCR ...
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bioRxiv - Cell Biology 2021Quote: Total cell lysates were prepared in Laemmli SDS-sample buffer supplemented with Protease Inhibitor Cocktail (Nacalai Tesque, Kyoto, Japan) and protein concentrations were quantified by the DC protein assay (Bio-Rad, CA, USA). Protein concentrations were normalized if necessary and boiled for 5 min with 2-mercaptoethanol ...