Labshake search
Citations for Bio-Rad :
1 - 50 of 271 citations for Malate dehydrogenase cytoplasmic MDH1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... and a corresponding sample of the cytoplasmic fraction were denatured in either 1x (Membrane fraction) or 4x (Cytoplasmic fraction) Laemmli sample buffer (Bio-Rad) before proceeding to SDS-PAGE and immunoblotting analysis.
-
bioRxiv - Immunology 2023Quote: ... and anti-neutrophil cytoplasmic antibody (ANCA) slides (29419, Biorad) were removed from pouches and allowed to equilibrate at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse anti glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:1000, BioRad). As secondary antibodies Horseradish Peroxidase (HRP ...
-
bioRxiv - Cancer Biology 2021Quote: ... 40μg of cytoplasmic protein was diluted in Laemmli Buffer (Bio-Rad) with Beta-mercaptoethanol ...
-
Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... nuclear or cytoplasmic extracts were added to 750 µl PureZOL™ (Bio-Rad) to extract the RNA ...
-
bioRxiv - Neuroscience 2023Quote: ... Seven whole flies per group were homogenized in cytoplasmic extraction buffer (Bio-Rad) and subsequently processed as instructed by the manufacturer.
-
bioRxiv - Cell Biology 2023Quote: ... at 50V for 85min (for cytoplasmic protein) or to 0.2µm nitrocellulose membranes (BioRad) at 30V for 70min (for histones) ...
-
bioRxiv - Microbiology 2022Quote: ... mouse-anti His (BIO-RAD; MCA1396GA), mouse-anti p65 (F-6 ...
-
bioRxiv - Microbiology 2023Quote: ... A mouse anti-His antibody (Biorad) was used as a primary antibody in a 1:500 dilution ...
-
bioRxiv - Cancer Biology 2019Quote: ... Nuclear and cytoplasmic protein fractions were measured by Bradford protein assay (BIO-RAD, Hercules, CA) and ∼50 µg of total protein was resolved by SDS-PAGE ...
-
Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Nuclear and cytoplasmic protein fraction concentrations were quantified by the Bradford protein assay (Bio-Rad).
-
bioRxiv - Biochemistry 2023Quote: ... both the cytoplasmic and WC fractions were reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad) and 12S mitochondrial rRNA primer was used for both the cytoplasmic and WC fractions (Supplementary Table 2) ...
-
bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
-
bioRxiv - Bioengineering 2023Quote: ... and Glyceraldehyde-3-Phosphate Dehydrogenase (Gapdh) were determined using SsoFast™ EvaGreen® Supermix (Bio-Rad), quantified using CFX96 C1000 thermocycler (Bio-Rad ...
-
bioRxiv - Neuroscience 2024Quote: ... Glycerinaldehyde-3-phosphate dehydrogenase (GAPDH) RNA was used as reference gene (Bio-Rad, assay ID: qRnoCID0057018).
-
bioRxiv - Neuroscience 2023Quote: ... Glycerinaldehyde-3-phosphate dehydrogenase (GAPDH) was used as a reference gene (Bio-Rad, assay ID: qRnoCID0057018).
-
bioRxiv - Molecular Biology 2022Quote: We subject all fraction lysates (total, nuclear, cytoplasmic, mitochondrial, and mitoplast) to Bradford Protein Assay (BioRad #5000006) to calculate protein concentration ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cytoplasmic extract prepared from the harvested cells was used for immunoprecipitation (IP) using ProteinG magnetic beads (Biorad). Beads were first equilibrated with IP buffer (25 mM Tris-Cl ...
-
bioRxiv - Cell Biology 2019Quote: ... Mitochondrial dehydrogenase activity within the electron transport chain was assessed by adding Alamar blue (BioRad, Kidlington, UK) to cells in culture for 4 h ...
-
bioRxiv - Neuroscience 2022Quote: ... Complementary DNA (cDNA) was synthesized from the cytoplasmic mRNA using iScript™ Advanced cDNA Synthesis Kit (Bio-rad). Target genes were then amplified with two PCR steps using the following primer pairs ...
-
bioRxiv - Plant Biology 2020Quote: ... 12.5 μL of SensiMix SYBR Hi-ROX (BIO-RAD) and DNAse/RNAse free water up to 15 μL ...
-
bioRxiv - Microbiology 2023Quote: ... and an anti-His-HRP conjugate antibody (Bio-Rad) was used in a 1:4000 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
-
bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected with 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad). The CA levels from cells were normalized relative to GAPDH levels ...
-
bioRxiv - Cell Biology 2021Quote: ... Nuclear (5 µg total protein) and cytoplasmic (10 µg total protein) fractions were boiled in Laemmli buffer (Bio-Rad), run on Criterion 4-20% Gels (Bio-Rad) ...
-
bioRxiv - Neuroscience 2020Quote: ... Thereafter proteins were denatured and SDS page was performed with 30μg of cytoplasmic proteins and 10μg of nuclear proteins on criterion TGX stain free gel 4-20% (Biorad, 5678094). Proteins were blotted on nitrocellulose membrane using semi-dry Transblot Turbo system (BioRad ...
-
bioRxiv - Neuroscience 2023Quote: Fractionation of nuclear and cytoplasmic fractions from Drosophila lysates was conducted with the ReadyPrep Protein Extraction Kit (Bio-Rad). Seven whole flies per group were homogenized in cytoplasmic extraction buffer (Bio-Rad ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were run on 7.5% Mini-PROTEAN gels for cytoplasmic proteins and 12.5% Mini-PROTEAN gels for histones (Bio-Rad, #4568025). Primary antibodies used ...
-
bioRxiv - Microbiology 2020Quote: One hundred μg of the different PAO1 cytoplasmic extracts were loaded onto conventional SDS-PAGE 10% Bis-Tris gels (mini-protean TGX Stain-free precast Gels, BioRad). The gel was stained with Coomassie blue and each lane was cut into 10 bands ...
-
bioRxiv - Molecular Biology 2023Quote: Equivalent amounts of 5-15 μg of protein for gel loading (cell lysates, mitochondrial/cytoplasmic fractions) were separated by SDS/PAGE on 4-20% Tris/glycine precast gels (BioRad) and transferred to PVDF membranes (BioRad) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The protein concentration of soluble cytoplasmic and membrane fractions was determined using the Bio-Rad Protein Assay (Bio-Rad Laboratories). Samples were boiled in 4X sample buffer ...
-
bioRxiv - Microbiology 2023Quote: Biological triplicates of either the secreted protein fractions or the pre-secretion cytoplasmic contents from cells normalized to OD600 4.0 were briefly run on a stain-free SDS-PAGE gel (Bio-Rad). After tryptic digestion and purification ...
-
bioRxiv - Molecular Biology 2024Quote: ... the total protein concentration in the nuclear and cytoplasmic extracts was measured using Protein Assay Dye Reagent Concentrate (Biorad, 5000006). 1 mg nuclear or 3 mg cytoplasmic extract per pulldown was incubated in triplicates with 15 μl of a 50% slurry of GFP-trap beads (ChromoTek ...
-
bioRxiv - Microbiology 2022Quote: ... mouse monoclonal His tag antibody (MCA1396GA, Bio-Rad, 1:1000), mouse 2A antibody (NBP2-59627 ...
-
bioRxiv - Genetics 2022Quote: ... 5% of the resulting lysate from the nuclear and cytoplasmic fractions were resolved by SDS-PAGE gel electrophoresis using 4-15% gradient gels (Bio-rad) and transferred to nitrocellulose membranes ...
-
bioRxiv - Microbiology 2023Quote: ... followed by incubation with mouse anti-His (1:1000, BioRad MCA1396GA) overnight at 4 °C and secondary antibody detection with IRDye800CW anti-mouse (1:5000 ...
-
bioRxiv - Microbiology 2024Quote: ... PorH-His immunoblots were performed on 16.5% tris-tricine gels (BioRad) using anti-His (mouse ...
-
bioRxiv - Microbiology 2020Quote: ... tuberculosis Rv1630 and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) housekeeping gene was performed using 200 ng RNA in a T100 Thermal Cycler (Bio-Rad). The reaction was carried out with Reverse Transcription Reagents (Applied Biosystems ...
-
bioRxiv - Plant Biology 2022Quote: ... Imaging was done using Hi-sensitivity chemiluminescence settings under ChemiDoc XRS+ (BioRad).
-
bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
-
bioRxiv - Developmental Biology 2021Quote: ... an affinity purified antibody generated against the SAX-7 cytoplasmic tail [gift of (Chen et al., 2001)] and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). For the loading control ...
-
bioRxiv - Cell Biology 2021Quote: ... Band density was normalized according to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) content and analyzed using Image Lab software (Bio-Rad, California, USA) (29).
-
bioRxiv - Neuroscience 2019Quote: ... BDNF exon IV and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA (as a reference gene) were amplified using the iScript RT-PCR iQ SYBR Green Supermix (BIORAD, Hercules, CA, USA) in a real-time quantitative PCR (qPCR ...
-
bioRxiv - Immunology 2023Quote: ... Serially diluted recombinant human IgG1 or human IgG4 (BioRad, Hercules, CA) with unrelated specificities were used for quantification in separate wells on the same plate.
-
bioRxiv - Developmental Biology 2021Quote: ... Quantification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) reference gene RNA level was performed to determine the efficiency of RNA extraction and qPCR amplification (Bio-Rad PrimePCR assay #qHsaCED0038674). SARS-CoV-2 viral loads below 40 RNA copies/mL were categorized as undetectable and set at 1.0 log10 RNA copies/mL.
-
A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... Human (Bio-Rad, USA) INSR - PrimePCR™ SYBR® Green Assay ...
-
A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... Human (Bio-Rad, USA). Resultant data was analysed using the proprietary LightCycler® Software 4.1 and Microsoft Excel ...
-
bioRxiv - Immunology 2020Quote: ... human CD68 (BioRad MCA5709), human ACE2 (Abcam ab15348) ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2023Quote: ... the His-FUS proteins were mixed with Profinity IMAC Ni2+-charged resin (Bio-Rad) for 30 min at 20 °C ...