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Citations for Bio-Rad :
1 - 50 of 120 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and analyzed by Western blot as previously described.21 Densitometry was performed to quantify the cleavage efficiency of full-length spike to S1/S2 subunits using ImageLab 6.0.1 (Bio-Rad #12012931). The average results of two experiments were presented.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plaques of FCoV were stained with specific antibodies (primary antibody: mouse anti feline coronavirus monoclonal antibody at 1:500 dilution solution, Bio-Rad; secondary antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... Initial screening for IgG antibodies was undertaken using an extensive panel of bacterial (Leptospira, Brucella) and viral antigens (paramyxovirus, filovirus, coronavirus, flavivirus, alphavirus) coupled to MagPlex beads (Bio-Rad, Australia) for multiplex screening ...
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bioRxiv - Microbiology 2023Quote: ... slides were incubated for 30 min at room temperature with primary mouse monoclonal anti-feline coronavirus antibody at 1:400 (BioRad, MCA 2194). The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).
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bioRxiv - Microbiology 2022Quote: ... Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10, Biorad) and anti-2A peptide (3H4 ...
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bioRxiv - Molecular Biology 2020Quote: Predesigned overlapping oligodeoxynucleotides (Fig S1) were assembled by PCR (BioRAD T100) using Primers F1 ...
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bioRxiv - Molecular Biology 2023Quote: ... a 160kD glycoprotein expressed by murine macrophages 235 (1:100, Bio-Rad clone C1:A3-1, Cat. MCA497) and rabbit anti-laminin (1:50 ...
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bioRxiv - Microbiology 2023Quote: gp120 or SOSIP glycoproteins were separated on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and transferred to a 0.45 μm nitrocellulose membrane (catalog number 1620115 ...
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bioRxiv - Neuroscience 2023Quote: ... Glycoproteins were eluted by boiling at 100°C for 10 minutes using 4x Laemmli sample buffer (Bio-Rad). Proteins were separated using a 4-12% Bis-Tris Protein SDS-PAGE Gel (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... the anti-Strep-tag antibody from Biorad, the anti-rabbit IgG-HRP and anti-mouse IgG-HRP antibodies from GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: - Mouse anti-V5 tag (Bio-Rad, MCA1360) used at dilution 1:5000 for Western Blot
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bioRxiv - Genomics 2023Quote: - Mouse anti-V5 tag (Bio-Rad, MCA1360) used at dilution 1:5000 for Western Blot
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bioRxiv - Cancer Biology 2022Quote: ... Primer nucleotide sequences (Table S1) were determined using Beacon Designer software (Bio-Rad) and their specificity was analyzed by BLAST and PCR dissociation curve analysis ...
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bioRxiv - Biochemistry 2020Quote: ... anti-His5-Tag IgG-HRP (MCA5995P, Bio-Rad), anti-FLAG-Tag (M2 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-His5-Tag IgG-HRP (MCA5995P, Bio-Rad), anti-FLAG-Tag (M2 ...
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bioRxiv - Plant Biology 2020Quote: ... using gene specific primers (Table S1) and iTaq Universal SYBR Green Supermix (Bio-rad). The actin gene (OsAct1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse anti-V5 tag antibody (SV5-Pk1, Bio-Rad) and secondary antibodies (Goat anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
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bioRxiv - Cell Biology 2020Quote: ... FCS was chelated with Chelex-100 resin (BioRad) and added to Ca2+ free FAD medium ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed using primers (Table S1) and Sso Advanced Universal SYBR Green Supermix (BioRad). Samples were processed as technical triplicates with the number of independent experimental replicates reported in the figure legends ...
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bioRxiv - Biophysics 2020Quote: ... Pure ClyA-AS type-I (12-mer) nanopores were obtained using native PAGE on a 4-15% gradient gel (Bio-Rad, Veenendaal, The Netherlands) and subsequent excision of the correct oligomer band.
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... lysates were incubated with 0.5μg V5-tag antibody (Bio-Rad) and ProteinG sepharose (GenScript ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal His tag antibody (MCA1396GA, Bio-Rad, 1:1000), mouse 2A antibody (NBP2-59627 ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using human-specific primers (Table S1) and iQ SYBR Green Supermix (Bio-Rad), with annealing temperature optimized by gradient PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected by electroporation in OPTI-MEM I using Gene-Pulser II (Bio-Rad) with 960 μF und 0.18 kV with 10 μg pcDNA3-FLAG-JUNB ...
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... The protein eluates were adjusted to 250 mM NaCl and loaded onto a Uno S1 (Bio-rad) ion exchange column ...
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bioRxiv - Cell Biology 2021Quote: ... Target gene cDNA was amplified using primers (Table S1) and Sso Advanced Universal SYBR Green Supermix (BioRad) on a CFX384 Touch Real-Time PCR Detection system (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT PCR was performed with gene-specific primers (Table S1) using iTaq universal SYBR Green Supermix (BioRad) for 45 cycles on CFX96 Real time system (BioRad) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Mouse anti-V5-Tag antibody (MCA1360GA, Bio-Rad, Hercules, CA, USA) at 1:2000 dilution was used to detect V5-tagged v-Src ...
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bioRxiv - Molecular Biology 2020Quote: ... goat anti-human IgG Fc (Biorad; 5211-8004; 1:2000) and rabbit anti-Vinculin (EPR8185 ...
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bioRxiv - Neuroscience 2020Quote: ... The post-nuclear S1 fraction (supernatant) was collected and protein concentration measured using the BCA protein assay (Biorad).
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bioRxiv - Microbiology 2021Quote: ... Western blots were performed using either mouse anti-V5 tag (Bio-Rad), 746-868 rabbit poly clonal IgG ...
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bioRxiv - Immunology 2023Quote: ... mice receive daily intradermal injections of CSF1-FC (Bio-Rad; PPP031) or PBS in contralateral hind paws (5µl per paw ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR analysis was performed using the primers in Table S1 on CFX384 Touch Real-Time PCR Detection System (BioRad). Data were analysed using the CFX Maestro Software (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... S1 nuclease digestion analyzed with PFGE was performed using a CHEF Mapper XA apparatus (Bio-Rad Laboratories, Hercules, CA). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL of cDNA was mixed with 2 μL of forward and reverse primers (2.5 μM each, Table S1,) and 1x SYBR Green Supermix (BioRad) for a final reaction volume of 20 μL ...
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bioRxiv - Plant Biology 2023Quote: ... with 7.5 ng cDNA and 200 nM primers (Table S1) on a CFX Opus 384 Real-time PCR System (BioRad). Mean PCR reaction efficiencies were calculated for each primer pair with LinRegPCR (Ruijter et al. ...
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bioRxiv - Genetics 2023Quote: ... For pulse-field gelectrophoresis of the S1- treated samples a CHEF mapper XA Pulse Field Electrophoresis System (Bio-Rad Laboratories Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... 15 μg total RNA from HEK293 cells were separated on 5% Criterion™ TBE polyacrylamide gels (#3450048, Bio-Rad) and transferred to positively charged nylon membrane ...
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bioRxiv - Genetics 2021Quote: ... to detect endogenous RPA1 or anti-V5 tag antibodies (mouse, BioRad; 1:100) to detect ectopically expressed RPA1 ...
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bioRxiv - Microbiology 2022Quote: ... The relative abundance of Spike transcripts was quantified by quantitative real-time PCR (qPCR) using CFX384 machine (BioRad) with a fluorescence reporter (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in FACS buffer with Fc-block (Human Seroblock, Bio-Rad). These samples were then re-pelleted and suspended in a cocktail of fluorophore-conjugated primary antibodies (Supp ...
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bioRxiv - Physiology 2024Quote: ... Fc-receptors were then blocked for 15 minutes using FcBlock (BioRad – BUF041A). Cells were pelleted by centrifugation (300 x g,5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Fc-receptors were then blocked for 15 minutes using FcBlock (BioRad – BUF041A). Cells were pelleted by centrifugation (300 x g ...
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bioRxiv - Bioengineering 2022Quote: ... 0.2 μM each of the forward and reverse primers (Supplementary Table S1) and iTaq Universal SYBR Green Supermix (Bio-Rad). All biological replicates were measured in technical duplicates.
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bioRxiv - Plant Biology 2021Quote: ... 10 µl PCR reactions were performed in technical duplicate with SensiFAST SYBR No-ROX (Meridian Bioscience) with 4 ng cDNA and 300 nM primers (Table S1) on a CFX96 Real-time PCR System (BioRad). Mean PCR reaction efficiencies were calculated for each primer pair with LinRegPCR (Ruijter et al. ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed using SYBR Green (Applied Biosystem – 4309155) and primers listed in Table S1 at an annealing temperature of 60°C on a CFX96 thermal cycler (Biorad). Generated data were analysed using the CFX Manager Software (Biorad).
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bioRxiv - Bioengineering 2019Quote: ... and the selected primers (Table S1, Supplementary Information) to perform qPCR using CFX Connect™ Real-Time System (Bio-Rad) under the following conditions ...