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Citations for Bio-Rad :
1 - 44 of 44 citations for Lassa Fever Virus Nucleoprotein NP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and anti-canine distemper virus nucleoprotein mouse monoclonal primary antibody (Biorad) in selected fox tissues.
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bioRxiv - Microbiology 2022Quote: ... or anti-Influenza A nucleoprotein (NP) mouse monoclonal (1:30) (MCA400, Bio-Rad). The next day ...
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bioRxiv - Immunology 2021Quote: ... NP (BioRad Cat#MCA400, 1:1000), and anti-β-actin (Sigma Aldrich Cat #A2228-100UL ...
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bioRxiv - Immunology 2021Quote: ... and NP (BioRad Cat#MCA400, 1:1000) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The fixed and permeabilized cell monolayer was stained with anti-nucleoprotein (Clone: AA5H, Bio-Rad Antibodies, UK) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.05% NP-40) and eluted with 2x Laemmli sample buffer (BioRad). Lysates and elutions were resolved by SDS-PAGE and western blotted in TBST using the following primary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... 0.05% NP-40) and eluted with 2× Laemmli sample buffer (Bio-Rad). 30 μg of whole-cell lysate or IP elutions were resolved on 4 to 15% mini-PROTEAN TGX gels (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.05% NP-40) and eluted by boiling in 2x Laemmli sample buffer (BioRad).
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bioRxiv - Neuroscience 2024Quote: ... The final virus samples were titered by digital droplet PCR (BioRad) using a REP-specific primer/probe set for libraries and a CMV primer/probe set for recombinant virus (Supplementary Table 2) ...
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bioRxiv - Microbiology 2020Quote: ... arenaviral NP samples were separated on pre-cast 7.5% SDS-PAGE gels (Bio-Rad). The mitochondrial import assay results were analysed by visualizing the proteins with the Coomassie stain (PhastGel Blue R ...
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bioRxiv - Biochemistry 2021Quote: ... Virus was inactivated by adding 50 μl of 2X Laemmli buffer (BioRad) and incubating at 95 °C for 10 minutes.
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bioRxiv - Microbiology 2021Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2022Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2021Quote: ... 30 μl of a primary antibody mix consisting of mouse anti-NP (Bio-Rad MCA400) and rabbit anti-JIP4 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: Concentrated PV or virus was mixed with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Immunology 2023Quote: ... (ii) genes in RNA virus infection panel of pre-designed human PrimePCR by BioRad; (iii ...
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bioRxiv - Immunology 2023Quote: ... or sensitized with 1 mg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA), or in combination for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... 40 µg of sample protein was diluted with NP-40 buffer and SDS Sample Buffer (Bio-Rad) and were then loaded into 10% precast gels (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
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bioRxiv - Biochemistry 2022Quote: ... The blood was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad) before use in the mosquitoes feeding process ...
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bioRxiv - Immunology 2021Quote: ... and TaqMan Fast Virus 1-step Master Mix) using CFX-96 (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... Grids were then blotted dry and suspended on a 25 µL drop of primary anti-NP antibody (Bio-Rad), diluted 1:500 in HN diluent ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were stained with primary antibody in blocking buffer at 4°C overnight: anti-NP (1:5000, OBT1555, Bio-Rad), anti-PA ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated virus was then inactivated and proteins denatured by heating with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and subsequently run on a 4-20% Mini-PROTEAN TGX protein gel (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: Total viral RNA was extracted from 0.1 ml virus stocks using Aurum Total RNA Mini Kit (Bio-Rad) and eluted in 50 μl elution buffer pre-heated to 70°C ...
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bioRxiv - Microbiology 2021Quote: ... All serum samples were confirmed as Dengue virus-positive by means of NS1 diagnostic ELISA test (Platelia, Biorad). Serum samples were filtered using Millipore 0.22 μm PES syringe filters ...
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bioRxiv - Immunology 2021Quote: ... The monolayer was fixed with 4% paraformaldehyde and permeabilized with Triton-X100 and stained with mouse anti-NP IgG1 (clone AA5H, Bio-Rad Antibodies) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Microbiology 2020Quote: ... Resulting cDNA was used for qPCR to detect GAPDH and NP mRNA with the iTaq Universal SYBR Green Supermix (Bio-Rad 1725121). Fold changes in NP mRNA were calculated using the ΔΔCT method.
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 5% BSA for 60min at RT and incubated with the primary antibody (anti-NP, Bio-Rad, 1/5000) for 45-60min shaking ...
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bioRxiv - Immunology 2024Quote: ... SDS-PAGE was performed using 10 μg of NP-40 extract protein mixed with an equal volume of 2X Laemmli buffer (BioRad cat#: 1610737) supplemented with 5% 2-mercaptoethanol (without boiling) ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0, 150 mM NaCl, 1% NP-40 [United States Biological, 19628], 0.5% deoxycholate [Sigma-Aldrich, D6750], 0.1% SDS [Bio-Rad, 1610302]) containing protease inhibitor cocktail and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in buffer containing 1% NP-40 and proteins were separated using SDS-PAGE (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad), transferred to low fluorescence PVDF membranes (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: The specific fluorescence produced during the formation of amplicons/ PCR product from the genomic RNA (converted to cDNA) of Rabies virus was identified by the detector in the qPCR machine (CFX CONNECT, BIORAD). The quantitative result is expressed in terms of relative quantification ...
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bioRxiv - Pathology 2022Quote: ... with an in-house full virus standard for determination of genome loads on a C1000 thermal cycler with the CFX96 Real-Time System (Biorad).
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bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
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bioRxiv - Microbiology 2022Quote: ... Absolute quantifications of virus RNA copies were estimated by modelling a Poisson distribution using QuantaSoft™ analysis software version 1.7 (Bio-Rad). Mean signal from N1 and N2 was extrapolated and expressed as viral RNA copies per microliter of supernatant ...
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bioRxiv - Cancer Biology 2022Quote: ... Virus quantification of KO/activation pool was done by droplet digital PCR (ddPCR) using QX200 Droplet Digital PCR System (Bio-RAD, #1864001).
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bioRxiv - Immunology 2019Quote: ... The calculation of IgG antibody concentration against each individual influenza virus strain rHA was performed by Bio-Plex Manager™ 6.2 software (Bio-Rad Co., CA).
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bioRxiv - Systems Biology 2021Quote: Differential expression of ten selected transcripts in response to virus at 3-L1 stage was examined using Droplet Digital PCR (ddPCR) (Bio-Rad, Hercules, CA). Because of the difficulty in obtaining a large amount of gut RNA samples and the limited sensitivity of conventional qPCR technique to detect gene expression at a low level ...
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bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.