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Citations for Bio-Rad :
1 - 50 of 479 citations for L Leucine N T Boc H2O 13C6 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... then to 97°C for 8 min in a thermocycler (BioRad). We centrifuged the samples and froze the supernatant for further use.
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bioRxiv - Biochemistry 2023Quote: 30% acrylamide:bis-acrylamide (99:1 ratio) solution was prepared by mixing 7.4 mL of 40% acrylamide (BioRad), 1.5 mL of 2% Bis-Acrylamide (Biorad ...
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bioRxiv - Genomics 2021Quote: ... They were then boiled for 5 minutes at 99°C after the addition of Laemmli buffer (Cat. #1410737, Biorad). Proteins were separated by SDS-PAGE and transferred to a membrane with a Trans Blot TURBO (Biorad) ...
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bioRxiv - Developmental Biology 2020Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Developmental Biology 2022Quote: ... lieberkuehni developmental stages were mixed in 2x Laemmli sample buffer with 10 % 2-β-mercaptoethanol and heated at 97 °C for 5 min before loading on a 12 % (w/v) TGX FastCast SDS/PAGE gel (Bio-Rad, USA). Membranes were blocked in 3 % (w/v ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then treated according to the instruction of the beads and the protein complexes were then eluted by incubating at 99°C for 5 minutes with Laemmli Sample Buffer (1610747, Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... 30-50 μg of total protein was boiled at 99°C for 5 minutes with the addition of Laemmli Sample Buffer (1610747, Bio-Rad) containing 1% 2-Mercaptoethanol in a total volume of 60-80 μl ...
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bioRxiv - Cell Biology 2022Quote: ... the mixes were boiled at 97°C for 5 min and loaded in a Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hércules, EE.UU) at 12% acrylamide/bis-acrylamide ...
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bioRxiv - Cell Biology 2023Quote: ... samples were boiled at 97°C for 5 min and run on a 12% Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hercules, USA) for 1 hour at 150V ...
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bioRxiv - Neuroscience 2019Quote: ... were heated at 99 °C for 3 minutes and subjected to 4-20% gradient SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad Laboratories, Inc.). Membranes were then blocked for 1 hour with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... heated in water bath for 5 minutes at 99°C and analysed by SDS-PAGE using Any-kD Mini-PROTEAN TGX Stain-Free gels (Bio-Rad, California, USA) in a 2-minute run for sample clean-up purposes ...
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bioRxiv - Biophysics 2022Quote: ... N,N,N’,N’-Tetramethylethylenediamine (TEMED, 1610801, Bio-Rad), Ammonium persulfate (APS ...
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bioRxiv - Neuroscience 2019Quote: ... N,N,N’,N’-Tetra-methyl ethylenediamine (TEMED, Bio-Rad, 161-0801), 10% ammonium persulfate (APS ...
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bioRxiv - Physiology 2022Quote: ... 1.5 μl H2O and 5 μl SsoAdvanced SYBR reagent (Bio-rad).
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bioRxiv - Cell Biology 2023Quote: ... 1.5 μl H2O and 5 μl SsoAdvanced SYBR reagent (Bio-rad).
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bioRxiv - Immunology 2019Quote: ... and N,N-methylene-bisacrylamide (Bio-Rad) to achieve the range of desired elasticities with a Poisson’s ratio of 0.5 ...
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bioRxiv - Immunology 2019Quote: ... and N,N-methylene-bisacrylamide (Bio-Rad) to achieve the range of elasticity ...
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bioRxiv - Genomics 2020Quote: ... and 6 µL H2O were mixed and incubated in a thermocycler (BioRad) using the following program ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.6 μL H2O and 11 μL 2× ddPCR Supermix (Bio-Rad #1863024). 20 μL of ddPCR mixes were used to generate droplets in a QX200 Droplet Generator (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed six times with PBS-T and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) (1:2000) (Bio-Rad) diluted in 2% horse serum/PBS for 1 hr at room temperature with agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... 5.4μl nuclease-free H2O (IDT) and 10μl ddPCR Supermix (no dUTP, Bio-Rad). The ddPCR was carried out using the QX200 ddPCR system according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... n,n’-methylene-bis-acrylamide (BIORAD, 2% w/v stock solution), N-6-((acryloyl)amino)hexanoic acid crosslinker (N6 ...
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bioRxiv - Plant Biology 2024Quote: ... a 20 μL mixture of 14N- and 15N-labeled samples was loaded onto a Tris-glycine 4-20% polyacrylamide gel (Mini-PROTEAN TGX; Bio-Rad) and the proteins of the sample separated by electrophoresis at 60 V for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... 0.035-0.25% N,N-methylenebisacrylamide crosslinker (Bis, 2% w/v stock, 161040, BioRad), 0.06% SDS (5% w/v stock in DI water ...
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bioRxiv - Microbiology 2023Quote: ... rinsed with H2O and imaged using Molecular Imager® Gel Doc™ XR System (Bio-Rad). Plaques were manually outlined and measured for size using ImageJ.
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bioRxiv - Cell Biology 2023Quote: ... concentration was fixed at 5% while N,N-methylene-bis-acrylamide (bis, Bio-rad) varied from 0.04% to 0.1% to attain different stiffnesses of the substrates ...
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bioRxiv - Cell Biology 2023Quote: ... concentration was fixed at 5% while N,N-methylene-bis-acrylamide (bis, Bio-rad) varied from 0.04% to 0.1% to attain different stiffnesses of the substrates ...
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bioRxiv - Bioengineering 2023Quote: ... 35 µL N,N-methylenebisacrylamide crosslinker (2% w/v bis-acrylamide Solution, 1610142, Bio-Rad), 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm ...
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bioRxiv - Bioengineering 2024Quote: ... 35 µL N,N-methylenebisacrylamide crosslinker (2% w/v bis-acrylamide Solution, 1610142, Bio-Rad), 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were eluted by boiling 10 min in 100 µL H2O containing 10% (V/W) chelex resin (BioRad), PK-digested for 30 min and then finally incubated 10 min at 95°C ...
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bioRxiv - Neuroscience 2022Quote: ... the reaction was stopped by a final methanol-chloroform-H2O precipitation and resuspended in laemmli buffer (Bio-Rad) for WB analysis.
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed in PBS-T or TBS-T and incubated with StarBright Blue secondary antibody (Bio-Rad) diluted in 5% milk in PBS-T for 30 min at room temperature in the dark and imaged using a Bio-Rad ChemiDoc imager.
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bioRxiv - Biochemistry 2022Quote: ... stock solution of 40% neutral acrylamide and N,N’-methylenebisacrylamide in a 19:1 ratio (Bio-Rad) were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt ...
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bioRxiv - Bioengineering 2024Quote: ... and subsequently destained in MilliQ H2O overnight before being imaged on a Gel Doc XR+ Molecular Imager (BIO-RAD). In-gel TAMRA fluorescence of quenchbodies following SDS-PAGE was analysed on an Amersham Typhoon Biomolecular Imager (Cytiva) ...
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bioRxiv - Genetics 2020Quote: ... for 2 × 1 h washes at room temperature and then stored overnight in MilliQ H2O before being visualized for myosin bands on a Chemi Doc MP (Biorad). Membranes were blocked at room temperature for 2 h using LI-COR blocking buffer with TBS (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... 20-100 µg of sheared chromatin used as input and ChIP beads were then boiled 10 min in 100 µL H2O containing 10% (V/W) chelex resin (BioRad), followed by Proteinase K (0.2 mg/mL)-digestion for 30 min at 55°C ...
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bioRxiv - Microbiology 2022Quote: ... Several 10-fold dilutions of each purified DNA were prepared in nuclease-free H2O and added to a reaction mixture containing ddPCR EvaGreen Supermix (Biorad). Forward (5′-GAATACATTCACATTGACCAATCAGAA-3′ ...
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bioRxiv - Plant Biology 2023Quote: ... diluted (1 μL in 50 μL milliQ-H2O) and deposited into sample wells onto a nitrocellulose membrane disposed into a 96-well Bio-Dot microfiltration apparatus (BioRad) previously soaked with in TBS (0.02 M Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... the gel was rinsed briefly with Milli-Q H2O and scanned using the gel imaging system (Bio-Rad ChemiDoc XRS+) in the Cy5 filter channel ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 hpf (n=40) or 48 hpf (n=25) embryos using the Aurum Total RNA Mini Kit (Bio-Rad). cDNA was then prepared with the RevertAid reverse transcriptase (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated in TBS-T/milk for 45 min and washed 3 × 5 min with TBS-T before detecting using a Chemidoc imager (Bio-Rad). NearIR-conjugated secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... extracted agarose gel parts were divided by cutting several times and purified via Freeze N′ Squeeze columns (Freeze N′ Squeeze, 7326165, BioRad) according to the manufacturer’s instructions using a benchtop centrifuge (Biofuge fresco ...
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bioRxiv - Microbiology 2020Quote: ... Each cDNA sample was diluted 1:15 in nuclease free H2O prior to qPCR analysis using specific primers and the iTaq Universal SYBR green mastermix (Bio-Rad). Primers for qPCR were designed using Primer3 software and include ...
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bioRxiv - Cell Biology 2021Quote: ... 1 ml of supernatant was diluted with 3 ml of distilled H2O and applied to freshly prepared Dowex columns (AG1-X8; Bio-Rad). Columns were washed twice with distilled H2O ...
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bioRxiv - Cancer Biology 2021Quote: ... Bound SRB dye was dissolved by adding 10 mM Tris-H2O to wells and OD was measured at 510nM with an iMARK microplate reader (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... cDNA was diluted 1:20 in RNase-free H2O and 3 µl dilution were used for qRT-PCR analysis using iTaq Universal SYBR Green (Bio-Rad) according to manufacturer’s recommendation ...
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bioRxiv - Biophysics 2019Quote: ... N-methylene-bis-acrylamide (Bio-Rad, Herculers, USA) are added ...
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bioRxiv - Neuroscience 2019Quote: The membrane was washed in 1x Tris buffered saline with 0.1% Tween-20 (TBS-T) and blocked in TBS-T with 2% (w/v) blotting grade blocker (Bio-Rad, Cat#: 1706404). Membranes were incubated in primary antibodies (Table 2 ...