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Citations for Bio-Rad :
1 - 50 of 296 citations for Interleukin 35 IL 35 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... 35 cycles using a thermal cycler (BioRad) and amplification conditions were 94°C for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... 35 µL of TEMED (cat. no. 161-0800, Bio-Rad) was added to the staining solution ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates (35 μg) were resolved on 10% SDS gels (Bio-Rad) and the protein was transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 35-50µg were loaded on a 4-20% TGX Gradient Gel (BioRad) and run at 100V for 1.5 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were eluted with 2 × 35 µl 1x Laemmli sample buffer (BioRad, 1610747) at 95°C.
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bioRxiv - Cell Biology 2022Quote: ... Lysates were normalised to a protein concentration of 35 μg by Bradford assay (BioRad). Samples were separated by SDS-PAGE on 4–12% Bis-Tris gels (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... 35 µL N,N-methylenebisacrylamide crosslinker (2% w/v bis-acrylamide Solution, 1610142, Bio-Rad), 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm ...
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bioRxiv - Bioengineering 2024Quote: ... 35 µL N,N-methylenebisacrylamide crosslinker (2% w/v bis-acrylamide Solution, 1610142, Bio-Rad), 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm ...
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bioRxiv - Biophysics 2019Quote: ... The sample was then loaded onto a 35 mL Biorex-70 column (Bio-Rad, Hercules, CA), washed with 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates (35-50 μg) were separated on SDS-PAGE and transferred to PVDF membrane (Bio-Rad), which were blocked with 5% milk for 1 hr and probed with primary antibody overnight at 4°C.
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bioRxiv - Developmental Biology 2023Quote: Cell fractionation was performed as described (35) and quantified using the DC protein assay (Bio-Rad). Nuclear extracts were separated by electrophoresis on 10% SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Biophysics 2024Quote: ... The sample was then loaded onto a 35 mL Biorex-70 column (Bio-Rad, Hercules, CA), washed with 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... goat anti-human IgG Fc (Biorad; 5211-8004; 1:2000) and rabbit anti-Vinculin (EPR8185 ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... 35 μg of protein lysate was loaded onto an SDS-PAGE gel (10% Pre-cast, Bio-Rad), ran for 1 h at 100 V and subsequently blotted onto a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2021Quote: ... 35 μg of protein lysate was loaded onto an SDS-PAGE gel (10% Pre-cast, Bio-Rad), ran for 1 h at 100 V and subsequently blotted onto a nitrocellulose membrane ...
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bioRxiv - Physiology 2022Quote: ... and run at 200V for 35 minutes in XT MES Running Buffer (Bio-Rad Cat# 161-0789). Gels were transferred to a PVDF membrane (Bio-Rad Cat# 162-0175 ...
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bioRxiv - Biochemistry 2022Quote: ... 35 µL of glucose-containing samples from terminated reactions were injected on a Aminex HPX-87P (Bio-Rad) column (300 × 7.8 mm ...
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bioRxiv - Molecular Biology 2019Quote: ... for 35 minutes at 200 V using MOPS buffer in a PowerPac™ HC Power Supply (Bio-Rad, Hercules ...
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bioRxiv - Microbiology 2019Quote: ... and 35 µl of each sample was electrophoresed through a Criterion 4-20% SDS-PAGE gel (Biorad 5671094) for 1.5 h at 250 volts ...
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bioRxiv - Physiology 2022Quote: ... and 72°C extension for 35 cycles using Taq polymerase and a DNA Thermal Cycler from Bio-Rad. The PCR products were resolved by electrophoresis in 1% agarose gel.
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bioRxiv - Biochemistry 2022Quote: Equal amounts of lysates (20-35 μg) were loaded onto a precast 4-12% gradient gel (Bio-Rad) and submitted to electrophoresis in NuPAGE buffer (150 V for 1.5 h) ...
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bioRxiv - Biochemistry 2023Quote: Electrophoresis was carried out for 35 minutes at 250 V using a Powerpac HV power supply (Bio-Rad). Some gels were then stained with Coomassie G250 Biosafe Stain ...
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bioRxiv - Immunology 2021Quote: ... IL-18 and IL-6 concentrations were measured using the Bio-Plex Pro Human Cytokine Assay (BioRad).
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bioRxiv - Molecular Biology 2021Quote: ... 35 μg of protein was resolved on 4-12% polyacrylamide gradient Criterion XT Bis-Tris Precast Gels (Biorad Laboratories) and transferred to a nitrocellulose membrane (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... This was then run for 35 min at 200 V on a 4-20% polyacrylamide gel (MiniPROTEAN TGX, BioRad) in 1x SDS running buffer (25 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Recombinant Bovine Interleukin-8 PBP039 and Goat anti Bovine Interleukin-8 Ab conjugated to biotin AHP2817B (all from Bio-Rad, protocol according to the manufacturer’s intructions).
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in FACS buffer with Fc-block (Human Seroblock, Bio-Rad). These samples were then re-pelleted and suspended in a cocktail of fluorophore-conjugated primary antibodies (Supp ...
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bioRxiv - Neuroscience 2021Quote: ... and the volumes corresponding to 35 μg were loaded onto TGX Stain-Free FastCast 12% polyacrylamide gels (Bio-Rad, USA) for electrophoretic separation ...
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bioRxiv - Cell Biology 2022Quote: ... 15-35 µg protein were loaded and separated by SDS-PAGE in 4–20% Mini-Protean TGX Precast gels (BioRad). Gels were transferred to PVDF membranes using the iBlot system (Thermo) ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR was carried out in triplicates and was performed at a 2-step reaction with 95°C denaturation and 56°C annealing and extension for 35 cycles on a CFX96 Real-Time System (BIORAD). Relative quantification of target genes was determined using Bio-Rad CFX manager 3.1 software ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
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bioRxiv - Microbiology 2022Quote: ... Ten microliters of each amplicon were separated on 2% agarose gel in 1X TAE buffer for 35 min at 135 volts and the amplified fragment detected using a GelDoc imager (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... Lysates (35 µg protein) were run on 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membranes (Bio-Rad, 1620115). After blocking for 10 min with 5% nonfat dried milk in PBS with 0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... were coated with 2 μg of plasmid DNA and introduced into plants laid on a 35-mm polystyrene dish using a helium gun device (PDS-1000/He, Bio-Rad) according to the manufacturer’s instructions (vacuum ...
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bioRxiv - Immunology 2020Quote: ... Measurements and analyses of secreted cytokines from a murine 35-plex kit were performed using a multiplex bead-based technology (Luminex) assay with a Bio-Plex 200 instrument (Bio-Rad) after 2-fold dilution of supernatants.
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bioRxiv - Cancer Biology 2021Quote: ... MA) and 35 μg of protein were loaded and size-fractionated by SDS-polyacrylamide gel electrophoresis (Bio-Rad Laboratories, Hercules, CA). Gels were then transferred to PVDF membranes ...
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bioRxiv - Microbiology 2023Quote: ... and the gel was run at 180 V for about 35 minutes (Mini-PROTEAN electrophoresis cell and PowerPac power supply, Bio-rad). Coomassie brilliant blue (Bio-Safe Coomassie stain ...
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bioRxiv - Plant Biology 2023Quote: ... during 35 minutes at 200 V (Mini-PROTEAN® Tetra Cell, 4-Gel System, Bio-Rad laboratories Inc, Hercules, CA, USA) and were electroblotted onto 0.45 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred to a 0.22 µm PVDF membrane by blotting at 24 V for 35 min using a Trans-Blot TurboTM system (BioRad, Feldkirchen, Germany). The membrane containing the proteins was blocked in 5% BSA in TBST at RT for 1 h to avoid unspecific binding of the antibodies ...
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bioRxiv - Microbiology 2023Quote: ... 35 µg of lysate was loaded on 10% SDS-PAGE after quantifying the protein concentration using Bradford assay kit (Bio-Rad). After transferring bands to nitrocellulose membrane Ponceau S staining was performed for checking equal loading of protein on SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... Fragments amplified using the FastGene Optima HotStart Ready Mix (Nippon Genetics) over 35 cycles in a C1000 Thermal Cycler (Bio-Rad) were subjected to agarose gel electrophoresis ...
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bioRxiv - Biochemistry 2024Quote: Rabbit RyR1 was purified following a previously described procedure 55 and reconstituted into amphipol A8-35 by mixing at 1:10 (w/w) with RyR1 followed by overnight detergent removal with BioBeads (Bio-Rad). For the Nb selection ...
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bioRxiv - Neuroscience 2019Quote: ... and run at 240 V for 35 minutes prior to semi-dry transfer to a 0.45 µm thick nitrocellulose membrane (Bio-Rad, Cat#: 1620115) at 16 V for 40 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a 0.45 µm nitrocellulose membrane for proteins larger than 35 kDa and to 0.1 µm nitrocellulose or 0.2 µm PVDF membranes for proteins smaller than 35 kDa for 30 min using Trans-Blot Turbo transfer system (Bio-Rad, 1704150) or for 2.5 h using wet transfer system (TG buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 20,000 x g for 20 min and the cleared lysate was passed through a 0.45-µm filter and loaded onto a XK-26/20 column (Cytiva) with 30-35 ml ceramic hydroxyapatite (Bio-Rad, cat no. 1582000). PlyCBWT was eluted from the column with three column volumes of 1 M sodium phosphate buffer (pH 7.2) ...
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bioRxiv - Cancer Biology 2024Quote: ... Dharmacon, Cambridge, UK) or siRNAs targeting MYC (D-003282-14 & D-003282-35, Dharmacon) using siLentFect transfection reagent (1703362, Biorad, Hercules, CA, USA) (see Table S4 for siRNA sequences) ...
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bioRxiv - Cell Biology 2023Quote: ... Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad, 171BL022M), Bio-Plex Pro Human Inflammation Panel 1 ...
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bioRxiv - Biochemistry 2021Quote: The purified SdGA was analyzed by SDS-PAGE using 12% (w/v) gels as described by Laemmli [35] with a Bio-Rad Mini Protean system (Bio-Rad, Hercules CA, USA). Gels were stained by Coomassie Blue and/or electro-blotted onto nitrocellulose membranes (HybondTM-ECLTM ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...