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Citations for Bio-Rad :
1 - 50 of 52 citations for Influenza Virus Hemagglutinin HA H1N1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... The calculation of IgG antibody concentration against each individual influenza virus strain rHA was performed by Bio-Plex Manager™ 6.2 software (Bio-Rad Co., CA).
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bioRxiv - Microbiology 2020Quote: ... influenza type b serogroup-specific antisera (Bio-Rad., Pastorex™ Meningitis). Streptococcus pneumonia (S ...
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bioRxiv - Microbiology 2022Quote: ... or anti-Influenza A nucleoprotein (NP) mouse monoclonal (1:30) (MCA400, Bio-Rad). The next day ...
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bioRxiv - Immunology 2019Quote: ... After quantifying the optimal virus-to-erythrocyte concentration (4HA units), serial two-fold dilutions of Fc, control IVIG (GammaGard, Baxter Healthcare) and polyclonal goat anti-influenza B (Biorad) were prepared ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was run through a hydroxyapatite (HA) column (type II 20 mM HA; Bio-Rad Laboratories) pre-equilibrated with 20 mM potassium phosphate ...
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bioRxiv - Microbiology 2022Quote: ... Influenza viral genome segments were reverse-transcribed using the iScript™ Reverse Transcription Supermix for RTqPCR (Bio-Rad, Cat. No. 1708840) and the Uni12 primer (5’-AGC AAA AGC AGG-3’ ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 10 μL of reconstituted representative influenza vaccine (i.e., AddaVax/Y-2) of Fluad Quadrivalent was mixed with Laemmli Sample Buffer (Bio-Rad, Hercules, CA) and β-mercaptoethanol (2% ...
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bioRxiv - Microbiology 2021Quote: ... and anti-canine distemper virus nucleoprotein mouse monoclonal primary antibody (Biorad) in selected fox tissues.
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bioRxiv - Neuroscience 2024Quote: ... The final virus samples were titered by digital droplet PCR (BioRad) using a REP-specific primer/probe set for libraries and a CMV primer/probe set for recombinant virus (Supplementary Table 2) ...
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bioRxiv - Microbiology 2023Quote: ... 25 µg or 50 µg of normalized protein for LapA-HA or MapA-HA tagged strains respectively was mixed with 4x Laemmli Sample Buffer (Bio-Rad) and 2-mercaptoethanol to a 1x working dilution and samples were boiled for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Virus was inactivated by adding 50 μl of 2X Laemmli buffer (BioRad) and incubating at 95 °C for 10 minutes.
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bioRxiv - Microbiology 2021Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2022Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2021Quote: Concentrated PV or virus was mixed with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Immunology 2023Quote: ... (ii) genes in RNA virus infection panel of pre-designed human PrimePCR by BioRad; (iii ...
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bioRxiv - Biochemistry 2020Quote: ... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
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bioRxiv - Biochemistry 2022Quote: ... The blood was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad) before use in the mosquitoes feeding process ...
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bioRxiv - Immunology 2021Quote: ... and TaqMan Fast Virus 1-step Master Mix) using CFX-96 (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... Concentrated virus was then inactivated and proteins denatured by heating with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and subsequently run on a 4-20% Mini-PROTEAN TGX protein gel (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: Total viral RNA was extracted from 0.1 ml virus stocks using Aurum Total RNA Mini Kit (Bio-Rad) and eluted in 50 μl elution buffer pre-heated to 70°C ...
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bioRxiv - Microbiology 2021Quote: ... All serum samples were confirmed as Dengue virus-positive by means of NS1 diagnostic ELISA test (Platelia, Biorad). Serum samples were filtered using Millipore 0.22 μm PES syringe filters ...
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bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Microbiology 2021Quote: The specific fluorescence produced during the formation of amplicons/ PCR product from the genomic RNA (converted to cDNA) of Rabies virus was identified by the detector in the qPCR machine (CFX CONNECT, BIORAD). The quantitative result is expressed in terms of relative quantification ...
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bioRxiv - Pathology 2022Quote: ... with an in-house full virus standard for determination of genome loads on a C1000 thermal cycler with the CFX96 Real-Time System (Biorad).
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bioRxiv - Plant Biology 2020Quote: ... which has a built-in Bio-Rad C1000 Thermal Cycler (Bio-Rad Laboratories GmbH, Munich, Germany) using Platinum SYBR Green qPCR SuperMix-UDG with ROX (Invitrogen by Life Technologies GmbH ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were blocked with 5% nonfat dry milk (HA blot) or EveryBlot Blocking Buffer (Bio-Rad) (SREBP1 blot ...
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bioRxiv - Immunology 2022Quote: ... subgenomic RNA and mice 18s rRNA has then been amplified using iQ SYBR green supermix (Biorad) and performed using CFX96 real time system (Biorad) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Approximately 2 ug of biotinylated HA was heated in non-reducing Laemmli buffer (Bio-Rad, #1610737) at 95°C for 5 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... which has a built-in Bio-Rad C1000 Thermal Cycler (Bio-Rad Laboratories GmbH, Munich, Germany). We used 18 μl of master mix and 2 μl of diluted (1:20 ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
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bioRxiv - Microbiology 2022Quote: ... Absolute quantifications of virus RNA copies were estimated by modelling a Poisson distribution using QuantaSoft™ analysis software version 1.7 (Bio-Rad). Mean signal from N1 and N2 was extrapolated and expressed as viral RNA copies per microliter of supernatant ...
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bioRxiv - Plant Biology 2023Quote: ... The anti-Myc and anti-HA antibody were used for detection by using ChecmiDoc MP machine (BioRad).
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bioRxiv - Cancer Biology 2022Quote: ... Virus quantification of KO/activation pool was done by droplet digital PCR (ddPCR) using QX200 Droplet Digital PCR System (Bio-RAD, #1864001).
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bioRxiv - Microbiology 2022Quote: ... Samples for both HA and IgG assays were then passed through a Micro-Bio spin column (Bio-Rad) and eluted in 50 μL 1x sample buffer for 5 min at room temperature ...
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bioRxiv - Systems Biology 2021Quote: Differential expression of ten selected transcripts in response to virus at 3-L1 stage was examined using Droplet Digital PCR (ddPCR) (Bio-Rad, Hercules, CA). Because of the difficulty in obtaining a large amount of gut RNA samples and the limited sensitivity of conventional qPCR technique to detect gene expression at a low level ...
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bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.
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bioRxiv - Cell Biology 2022Quote: ... Twenty (HA blot) or forty (SREBP1 blot) micrograms of protein were mixed with Laemmli sample loading buffer (Bio-Rad), heated to 37 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: Iron was removed from has samples using Chelex® 100 Chelating Ion Exchange Resin (Bio-Rad, Hercules, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were immunoprecipitated again via the HA-tag and analyzed by 4-15% non-reducing gradient SDS-PAGE (BioRad) and processed as before.
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bioRxiv - Neuroscience 2022Quote: Two thirds of eluted protein sample from HA Immunoprecipitation were run on a 4-15% Mini-PROTEAN (Bio-Rad) gel to separate proteins and remove synthetic HA peptide ...
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bioRxiv - Biophysics 2021Quote: ... protein was further purified by a Hydroxyapatite column (HA) connected to an NGC Medium-Pressure Liquid Chromatography System (BIO-RAD). A gradient form 5 mM NaPi ...
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bioRxiv - Microbiology 2021Quote: ... The nitrocellulose strips were blocked in PBS with casein for 30 mins at room temperature and then probed for 1 hour with monoclonal antibodies (mAb) recognizing H3 HA (clone 36/2 (26)) or IAV M1 matrix protein (clone MCA401, BioRad). Strips were washed and incubated for 1 hour with a horse radish peroxidase (HRP)-conjugated secondary immunoglobulin (DAKO) ...
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bioRxiv - Genetics 2023Quote: ... Input gel samples were taken before addition of HA beads: 30 μL of lysate was added to 10 μL 4X Laemmli sample buffer (BioRad) and boiled at 95°C for 5 minutes.
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bioRxiv - Plant Biology 2024Quote: ... in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad) and the ChemiDoc Touch imaging system (Bio-Rad).
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bioRxiv - Genomics 2020Quote: ... Primers and probes used for IAV-M, IBV-HA were previously reported (World Health Organization, 2017) and synthesized by Bio-Rad Laboratories ...
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bioRxiv - Genetics 2023Quote: ... primary antibodies – anti-TY1 and rabbit anti-HA (GTX115044) – and fluorescent secondary antibodies – anti-mouse Starbright Blue 520 (Bio-Rad 12005867) and anti-rabbit Starbright Blue 700 (Bio-Rad 12004162 ...
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bioRxiv - Microbiology 2019Quote: ... and FLAG-cMCF-HA recovered as described above were prepped using the Bio-Rad ReadyPrep 2-D Cleanup Kit (Bio-Rad 1632130). The sample was then loaded onto 11 cm pH 3-10 immobilized pH gradient (IPG ...
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bioRxiv - Systems Biology 2021Quote: ... RT reactions were performed in raw or purified HA-bmp2b mRNA and purified embryos mRNA using iScript™ Reverse Transcription Supermix (Bio-rad, # 1708840), followed by digital PCR with TaqMan hydrolysis probes using the QX100TM Droplet Digital PCR System ...