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Citations for Bio-Rad :
1 - 50 of 2161 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were boiled with 2× Laemmli sample buffer (Bio-Rad) containing 2.5% β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... The lysate was added to 2 chromatography columns (7321010, BioRad) packed with 25 mL each of chitin resin (S6651S ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates were prepared by utilizing 2 × Laemmli buffer (Bio-Rad). Proteins from mouse brain tissue were separated on 8%–16% and 4%–20% gradient SDS-PAGE gels before being transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were diluted with 2× Laemmli sample buffer (Bio-Rad) and boiled at 95°C for 10 min and then underwent western blotting ...
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bioRxiv - Molecular Biology 2023Quote: ... These markers were amplified in standard simplex PCR conditions using a Bio-Rad T100 thermal cycler (Bio-Rad, Pleasanton, CA, USA). Each PCR reaction contained 2.0 μl of DNA ...
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bioRxiv - Neuroscience 2023Quote: ... IL-18 levels were measured using ProcartaPlex Simplex bead sets (eBioscience) and a Bio-Plex instrument (Bio-Rad, Hercules, CA, USA). Median IL-18 concentrations and interquartile ranges were calculated for cALD and control groups ...
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bioRxiv - Cancer Biology 2023Quote: ... purified soluble TAG72 antigen (BioRad) was plated in duplicate at indicated TAG72 units overnight at 4°C in 1X PBS in 96-well flat bottom high-affinity plates (Corning) ...
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bioRxiv - Cell Biology 2021Quote: Cells lysates were separated on 4-2% gradient SDS-polyacrylamide gels (Biorad), transferred to polyvinylidene fluoride (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates and immunoprecipitates on beads were resuspended in 2× Laemmli buffer (Bio-Rad) and subjected to SDS-PAGE and western blot analysis.
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bioRxiv - Microbiology 2024Quote: ... the beads and total lysate were resuspended in 2× Laemmli sample buffer (Bio-Rad) supplemented with 5% 2-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... NS1 antigen levels were measured using the Platelia™ Dengue NS1 Antigen kit (Cat No. 72830, Bio-Rad, France) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
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bioRxiv - Cell Biology 2020Quote: Cell lysates were harvested by directly lysing the cells with 2 × sampling buffer (Bio-Rad). Proteins were separated by SDS-PAGE ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were mixed 50:50 with 2X Laemmli Buffer and 2-mercaptoethanol (Bio-Rad Laboratories). Samples were run on a SDS–PAGE gel and transferred to nitrocellulose membranes before being blocked in a 10% skim milk powder/PBS solution for 60 min ...
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bioRxiv - Immunology 2023Quote: ... Labeled glycans were dissolved in HPLC grade water (FisherScientific) and excess unbound APTS removed using Agencourt CleanSEQ beads (non-antigen specific glycans) and Bio-Gel P-2 (Bio-rad) size exclusion resin (RBD-specific glycans) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
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bioRxiv - Biochemistry 2022Quote: Cell lysates were diluted to 2 µg/µl in Laemmli buffer (Bio-Rad, Hercules CA, 1610747) and heated at 95ºC for 5 minutes and resolved using a 4-20% tris-glycine gel (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... 5% of the lysates were boiled with 2 x laemmli buffer (BIO-RAD, Hercules, CA, USA) and used as input ...
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bioRxiv - Microbiology 2021Quote: ... serial dilutions of recombinant NS1 Antigen (Ag) (Bio-Rad) was used ...
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bioRxiv - Neuroscience 2022Quote: ... Solid phase specificity was confirmed with dot blots of serial antigen dilutions (2 μL) applied to nitrocellulose membrane (Bio-Rad 0.45 µm; Fig. 1b). Membranes were allowed to air dry (1 h ...
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bioRxiv - Immunology 2019Quote: HIV antigens were covalently conjugated to polystyrene beads (Bio-Rad), and binding of IgG to the bead-conjugated HIV-1 antigens was measured in RM plasma samples (29) ...
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bioRxiv - Immunology 2019Quote: ... HIV-1 antigens were conjugated to polystyrene beads (Bio-Rad) as previously described (18) ...
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bioRxiv - Molecular Biology 2022Quote: ... Antigens were visualized using enhanced chemiluminescence (BioRad Clarity ECL; 1705061).
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bioRxiv - Cell Biology 2020Quote: ... Equal volume of cell (representing 1/20 of the total lysate) and EVs lysates (representing total lysate) were resolved by SDS-PAGE and transferred to PVDF membranes (BioRad, 162-0177), blocked in Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Antigen-antibody complexes were visualized using enhanced chemiluminescence substrate (Bio-Rad) on the Fluorchem E imaging system (ProteinSimple).
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bioRxiv - Microbiology 2023Quote: ... The antigen concentration was determined by Bradford’s assay (Bio-Rad, USA). The mice were pre-bled before immunization and boosted 2 times ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen-antibody complexes were visualized using enhanced chemiluminescence substrate (Bio-Rad) on a FluorChem E detection system (ProteinSimple) ...
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bioRxiv - Biochemistry 2019Quote: Trypanosome whole cell protein lysates (2×106 cells per well) were separated using SDS-PAGE (10% gels) and transferred by semi-dry blotting (BioRad) for 45 min at 25 V to PVDF membranes ...
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 µl of the lysates were subject to 1 ml of 0.2x Bradford protein dye reagent (Bio-Rad #5000006) for protein quantification ...
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bioRxiv - Developmental Biology 2022Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Nuclear and cytosolic fractions of iPSCs-differentiated cells were acquired using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific #78833 ...
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bioRxiv - Cell Biology 2023Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Protein samples were loaded on 4-15% polyacrylamide gels (BioRad ...
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bioRxiv - Neuroscience 2019Quote: ... Antigen-antibody complexes were visualized by using enhanced chemiluminescence substrate (Bio-Rad) on a FluorChem E detection system (ProteinSimple) ...
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bioRxiv - Microbiology 2021Quote: ... and anti-canine distemper virus nucleoprotein mouse monoclonal primary antibody (Biorad) in selected fox tissues.
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bioRxiv - Neuroscience 2024Quote: ... The final virus samples were titered by digital droplet PCR (BioRad) using a REP-specific primer/probe set for libraries and a CMV primer/probe set for recombinant virus (Supplementary Table 2) ...
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bioRxiv - Immunology 2021Quote: ... Lysates were finally adjusted to a final concentration of 2 µg/µl with 4X Laemmli buffer (Bio-Rad, Hercules, CA, USA) containing 10% β-Mercaptoethanol.
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bioRxiv - Neuroscience 2021Quote: ... In a water bath samples were boiled for 2 minutes and 12 ul of each lysate were loaded on two separate gels (Biorad, #4568096), one each required for parallel HTT and pS13 HTT detection ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells lysates were separated by BioRad mini protean TGX gels 4-20% ...
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bioRxiv - Microbiology 2021Quote: ... The antigen-antibody complex was detected using Clarity Western ECL substrate (Bio-Rad), and ChemiDoc MP Imaging System according to the manufacturer’s manuals.
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bioRxiv - Biochemistry 2021Quote: ... The antigen-antibody interaction was developed with Clarity Western ECL Substrate (#1705060, Biorad) and detected in an Omega Lum C detector ...
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bioRxiv - Immunology 2021Quote: ... and antigen-antibody complexes were analyzed on BioPlex 200 multiplexing systems (Bio-Rad) for IgG binding ...
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bioRxiv - Microbiology 2021Quote: ... vivax antigens to non-magnetic COOH microspheres (Bio-Rad Laboratories, Inc., California, USA) was performed as described [52] ...
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bioRxiv - Immunology 2019Quote: ... HIV-1 antigens were covalently coupled to carboxylated fluorescent beads (Bio-Rad Laboratories) and IgG binding to the bead-coupled antigens was measured ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were added to a 2 mL bed of Ni-NTA agarose resin in a 20 mL column (Bio-Rad 7321010) equilibrated with wash buffer (50 mM Tris-HCl (pH 8) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates were boiled in loading buffer [1:9 ratio of 2-mercaptoethanol:4x Laemmli Sample Buffer (Cat.#1610747; Bio-Rad Laboratories)] for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... Virus was inactivated by adding 50 μl of 2X Laemmli buffer (BioRad) and incubating at 95 °C for 10 minutes.
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bioRxiv - Microbiology 2021Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2022Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...