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Citations for Bio-Rad :
1 - 50 of 204 citations for Glutathione S transferase Class mu 26 kDa Isozyme GST Schistosoma Japonicum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... GST fusions were stained with Ponceau S or 0.2% Coomassie Brilliant Blue (BIO-RAD Inc.). For co-transfection studies ...
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bioRxiv - Microbiology 2020Quote: ... Concentration of GST-CsrA and GST-CsrAD41K was determined by Bradford assay (Biorad).
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Immunology 2022Quote: 2 µg of GST or GST-IFITM3 protein was loaded into a 12% acrylamide Criterion XT Bis-Tris Precast Gel (Bio-Rad). Electrophoresis was performed with NuPage MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... Blots were processed using ImageLab (Version 6.0.0 build 26, BioRad), final figures were assembled in PowerPoint (Microsoft).
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bioRxiv - Microbiology 2020Quote: ... 26), respectively, and PCR cycling conditions as described previously (26, 43) on a Bio-Rad T100 thermal cycler (Bio-Rad Laboratories Inc, Hercules, CA, USA). The expected amplicon sizes were approximately 330 bp for the 16S rRNA gene fragment and 431 bp for the pheS gene fragment ...
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bioRxiv - Neuroscience 2023Quote: ... and mouse anti-MHC Class I RT1A (#MCA51G, 1:100, clone: OX-18, 1:100, Bio-Rad). Sequential imaging was performed on an Olympus FluoView confocal laser-scanning microscope (FV1200 ...
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bioRxiv - Genetics 2020Quote: ... myoglobin (17 kDa) and vitamin B12 (1.35 kDa) according to the manufacturer’s instructions (BioRad, 151-1901).
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bioRxiv - Bioengineering 2024Quote: ... Premixed molecular weight markers (Protein standards, ladder, 250 kDa - 10 kDa, cat#1610374, BioRad Laboratories Inc.) were used as a reference control to confirm molecular masses of the protein bands in the sample lanes ...
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bioRxiv - Molecular Biology 2019Quote: ... and primer (26) was used in a T100 thermocycler (BIORAD, CA, USA) under PCR conditions similar to those described by Shoji et al (13).
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bioRxiv - Molecular Biology 2023Quote: ... myoglobin (17.5 kDa) and vitamin B12 (1.35 kDa) was used to calibrate the column (Bio-Rad, CA, USA). The curves were plotted using GraphPad Prism (Dotmatics ...
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bioRxiv - Plant Biology 2024Quote: ... 26S (SUN) Universal probes for RT-qPcR and iTaq Mastermix (Biorad, Hercules,CA).Primers and probes used in this study are listed in Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... B and S (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2020Quote: ... and 5 mM glutathione (EMD) in a Gene Pulser Xcell (Bio-Rad Laboratories), with the following settings ...
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bioRxiv - Cell Biology 2021Quote: ... resolved on 4–20% polyacrylamide gels (15-well, Invitrogen; 26-well, Bio-Rad Laboratories), and transferred to PVDF (EMD Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... and resolved on 4-12% Bis-Tris XT Precast 26-well gels (Bio-Rad). Proteins were transferred to polyvinylidene difluoride membranes (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... GS-resin bound GST-βarr was transferred into Econo columns (Biorad, Cat. no.) and washed rigorously with wash buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... Each column was calibrated using 660 kDa to 1.35 kDa molecular weight protein standards (thyroglobulin, IgG, ovalbumin, myoglobin, and vitamin B12) (Bio-Rad). Following peak analysis by SDS-PAGE ...
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bioRxiv - Pathology 2020Quote: ... The correct size of the purified RBD-scFv protein (52.4 kDa → ∼57 kDa with glycosylation) was confirmed by protein gel electrophoresis using Mini-PROTEAN TGX Precast Gels 4-20% (Bio-RAD).
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A new vulnerability to BET inhibition due to enhanced autophagy in BRCA2 deficient pancreatic cancerbioRxiv - Cancer Biology 2023Quote: ... S (Bio-Rad, 5000113, 5000114, 5000115). The cell extracts were mixed with 4X LDS sample buffer (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... or Tris-Glycine gels (Bio-Rad; MW>100 kDa) and run at 160 V for 50 min ...
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bioRxiv - Microbiology 2021Quote: Supernatant glucose and arabinose concentrations were analyzed by high-performance liquid chromatography (Waters Acquity UPLC® H-Class System) using an ion-exchange Aminex HPX-87H column (7.8 × 300 mm, Bio-Rad Laboratories N.V.). The analysis was carried out with an isocratic flow rate of 0.6 mL min−1 for 25 min at 50°C ...
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bioRxiv - Cell Biology 2020Quote: ... samples were resolved on 4–20% polyacrylamide gels (15-well, Invitrogen; 26-well, Bio-Rad Laboratories), and transferred to PVDF (EMD Millipore ...
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bioRxiv - Biophysics 2022Quote: ... with a Nuvia S column (Bio-Rad) and using a fast protein liquid chromatography (FPLC ...
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bioRxiv - Biochemistry 2021Quote: ... cation exchange (BioRad UNOsphere Rapid S column), and size exclusion chromatography (BioRad Enrich SEC 70 column or Cytiva HiLoad 16/600 Superdex 75 ...
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bioRxiv - Biochemistry 2024Quote: ... An ENrichTM S 5x50 column (Bio-Rad) was equilibrated with low-salt (LS ...
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bioRxiv - Molecular Biology 2019Quote: ... Transformation was done chemically with heat shock at 42 °C for 30 s or by electroporation with 0.1 cm gap cuvettes at 1.8 kV for 1 s (Gene Pulser, Bio-Rad), depending on the competent cells required ...
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bioRxiv - Neuroscience 2020Quote: ... Five micrograms of protein were loaded into 26-well 10% Bis-Tris gels (BioRad Laboratories, Hercules, CA), resolved under reducing conditions using 1 × 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were loaded per well of a 26 well 4-15% Criterion Precast Protein Gel (BioRad Cat#5671085) and proteins were ran at 200 V through the gel before being transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2023Quote: ... using 10% mini gels and 26-well pre-cast gels (Criterion TGX, Bio-Rad, Hercules, CA, USA), which enable separation in a broad range of molecular weights ...
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bioRxiv - Neuroscience 2022Quote: ... Antisera to GST-MTD was affinity purified by pre-absorption on an Affi-Gel (Bio-Rad) column coupled with GST ...
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bioRxiv - Microbiology 2021Quote: ... and switch time linearly ramped from 1 s to 5 s over 14-15 h (CHEF DR III, Bio-Rad). DNA bands were visualized by staining the gel with a solution of 0.5 µg/mL ethidium bromide ...
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bioRxiv - Physiology 2019Quote: ... initiation at 95°C/10 s followed by 45 amplification cycles (60°C/15 s) on the CFX Thermal cycler (Biorad).
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bioRxiv - Systems Biology 2021Quote: ... followed by 15 s at 95 °C and 60 s at 58 °C for 40 cycles using Biorad CFX384 thermocycler (Biorad). The mRNA levels of genes encoding cytokine expression were normalized relative to the mean levels of the housekeeping gene and compared using the 2−ΔΔCt method as described previously2 ...
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bioRxiv - Plant Biology 2024Quote: ... using 45 cycles of 15 s at 95°C and 30 s at 60°C in a CFX96 cycler (Biorad). Primers are listed in Additional file 1.
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bioRxiv - Molecular Biology 2020Quote: ... FLAG (EGFR) signals were quantified for the FLAG immunoprecipitation using Image Lab (BioRad Laboritories, version 6.0 build 26). Lanes and bands were manually defined using the volume tool ...
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bioRxiv - Biochemistry 2021Quote: ... A broad-range protein marker (10-250 KDa, Kaleidoscope # 1610375, Biorad) was included ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... then loaded into an Any kDa Mini-Protean TGX gel (BioRad). After electrophoresis the gels were transferred to nitrocellulose membranes using the TransBlot TURBO system (BioRad) ...
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bioRxiv - Bioengineering 2020Quote: ... followed by 40 cycles of 95°C for 5 s and 60°C for 30 s (Bio-Rad CFX manager 3.1). Quantification of mRNA levels of different genes was performed using the 2−ΔΔct method ...
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bioRxiv - Cancer Biology 2024Quote: ... 40 cycles of 95 °C for 5 s and 60 °C for 10 s using a CFX Connect Real-Time PCR System (Bio-Rad). The relative expression levels of mRNA were calculated using the 2−ΔΔCt method.
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bioRxiv - Cancer Biology 2019Quote: ... followed by 5 s at 95°C and 30 s at 60°C for 40 cycles using an Biorad CFX384 system (Biorad, Hercules, CA). The mRNA levels of each gene were normalized relative to the mean levels of the two housekeeping genes and compared with the data obtained from unstimulated ...
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bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Cell Biology 2020Quote: ... Transfer efficiency was checked with reversible Ponceau S (BioRad) staining ...
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bioRxiv - Biophysics 2021Quote: ... Micro Bio-Spin 6 Columns (molecular weight cutoff 6 kDa; Bio-Rad) were used at 1,000 × g and 4 °C to exchange purified protein samples to 250 mM ammonium acetate (99.99 % purity ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified over the corresponding GST-LRRCC1 fusion bound to Affi-Gel 10 resin (Bio-Rad). Other primary and secondary antibodies used in this study are listed in Table 1.
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bioRxiv - Biochemistry 2020Quote: ... The BRPF1 bromodomain protein was purified on glutathione agarose resin in a 25 mL Econo-Column® (Bio-Rad) by first washing the beads extensively with wash buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The nitrocellulose strips were blocked in PBS with casein for 30 mins at room temperature and then probed for 1 hour with monoclonal antibodies (mAb) recognizing H3 HA (clone 36/2 (26)) or IAV M1 matrix protein (clone MCA401, BioRad). Strips were washed and incubated for 1 hour with a horse radish peroxidase (HRP)-conjugated secondary immunoglobulin (DAKO) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were deposited onto 26-well 12% Bis-Tris Criteroin XT precasted gels (Bio-Rad Laboratories Inc., Hercules, CA, USA) and electrotransferred onto a nitrocellulose membrane with a semidry electrotransfer system (Schleicher & Schuell BioScience ...
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bioRxiv - Microbiology 2022Quote: ... Chemiluminiscence signal was acquired through ChemiDoc XR S+ (Bio-Rad).