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Citations for Bio-Rad :
1 - 50 of 8982 citations for Fatty acid binding protein FABP3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The binding to protein G magnetic beads (Biorad) was performed for 1 hour at 4 ºC (with previous washes with a RIPA buffer) ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... Protein binding was detected by using ECL substrate (BioRad) and visualised by ImageQuant4000 (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... The protein concentration was measured using the Bradford dye-binding protein assay (Bio-Rad), using known concentrations of bovine serum albumin as external standards.
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... The protein concentration was determined using the Bradford protein dye binding assay (#5000113 + #5000114, Bio-Rad). 50μg of protein was separated by electrophoresis in an SDS-polyacrylamide gel which was then transferred using the semi dry method ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were quantified with the Coomassie dye binding method (BioRad, 500-0006) as described by the manufacturer.
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bioRxiv - Cancer Biology 2020Quote: ... Unspecific binding was blocked by using PBS with 5% human AB serum (Bio-Rad, Germany) and 5% mouse serum (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentrations were determined with the bicinchoninic acid (BCA) protein kit (BioRAD).
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bioRxiv - Biochemistry 2020Quote: ... Protein concentrations were determined by the dye-binding method of Bradford assay (Bio-Rad) and bovine serum albumin as the calibration standard ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... the His-FUS proteins were mixed with Profinity IMAC Ni2+-charged resin (Bio-Rad) for 30 min at 20 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were then transferred to fresh low- protein binding tubes and antibody-protein complexes were eluted by incubating in 60 μL 1x Laemmli Sample Buffer (Biorad, cat ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged fusion protein was purified using a Ni-NTA column (Bio-Rad, USA). Protein was eluted in elution buffer [500 mMNaCl ...
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bioRxiv - Microbiology 2024Quote: ... Detection of His-tagged proteins was performed with the ClarityTM Western ECL substrate (Biorad, USA) and using a Versadoc (Biorad ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected by an HRP-conjugated anti-human IgG (Fc) CH2 Domain antibody (Bio-Rad MCA647P) used at a 1:5,000 dilution at RT for 1 h and developed as described above.
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bioRxiv - Cancer Biology 2020Quote: ... lysate concentrations were adjusted to equal protein concentrations using the bicinchoninic acid (BCA) protein assay (Biorad). Equal amounts of protein were mixed to a final concentration of 1x reducing sample buffer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was determined by the bicinchoninic acid assay (Bio-Rad), and the purified proteins were stored at −80°C until used.
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bioRxiv - Immunology 2023Quote: ... Colorimetric (standards) and chemiluminescent detection (His-tagged proteins) were performed using the ChemiDoc Imager (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was estimated on the lysates using a bicinchoninic acid protein assay Kit (5000111, Bio-Rad). Equal protein concentrations for each of the samples were incubated with Myc antibodies overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-Rad, Marnes-La-Coquette, France). Mouse IgG1 isotype control was purchased from Miltenyi Biotec (Bergisch Gladbach ...
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bioRxiv - Plant Biology 2023Quote: ... Native protein extracts from E.coli or Synechococcus ΔCS were incubated in IMAC HEPES binding buffer supplemented with potential effector molecules and Ni-charged iminodiacetic acid Profinity™ IMAC Resin (Bio-Rad, USA) on a low speed rotator for 1 h at 23°C ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total protein lysate concentrations were quantified using a bicinchoninic acid (BCA) assay (BioRAD) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... protein content was determined with a bicinchoninic acid reagent (Bio-Rad, Hercules, CA). Equal loading was verified in a twin run of electrophoresed individual strips of protein stained with Ponceau S (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... Ten µl of the supernatant were used to determine protein concentrations by the dye-binding Bradford assay (Bio-Rad). Twenty-five µg of total proteins in a final volume of 100 µl were pipetted into a white 96 well plate (Greiner Bio-One ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was measured using a bicinchoninic acid (BCA) protein assay (Pierce) or Bradford assay (Bio-Rad, Cat. No. 5000006). Proteins were prepared in 1× Laemmli buffer (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... The total protein concentration was determined using the Bicinchoninic Acid (BCA) assay (Bio-Rad). Then ...
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bioRxiv - Cell Biology 2022Quote: ... a DNA-binding fluorescent dye (BioRad, SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cell Biology 2022Quote: ... Total cell lysates were prepared with icecold lysis buffer and the protein concentration was measured by Coomassie binding (Bio-Rad). Equal amounts of protein were added to each well of a G-LISA plate and incubated for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: All binding assays were performed on a ProteOn XPR36 SPR Protein Interaction Array System (Bio-Rad Laboratories, Hercules, CA, USA). The instrument temperature was set at 25 °C for all kinetic analyses ...
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bioRxiv - Neuroscience 2021Quote: ... The Aurum™ Total RNA Fatty and Fibrous Tissue Kit (732-6830; Bio-Rad) was used to extract total RNA from the frontal cortex following the manufacturer’s instructions ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Incorporation of radioactive amino acids was determined by liquid scintillation counting and normalized to the cellular protein content (quantified with DC Protein Assay (Bio-Rad)).
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bioRxiv - Microbiology 2022Quote: ... mouse-anti His (BIO-RAD; MCA1396GA), mouse-anti p65 (F-6 ...
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bioRxiv - Microbiology 2023Quote: ... A mouse anti-His antibody (Biorad) was used as a primary antibody in a 1:500 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed four times with binding buffer containing 0.1% Tween-20 and bound proteins were eluted with 2X Laemmli Sample Buffer (Bio-Rad Laboratories). Eluted proteins were subjected to electrophoresis on a 17% resolving gel at 120V for 2 hrs and transferred to PVDF membrane at 180mA for 2 hrs and immunoblotted with His-Probe-HRP (Pierce ...
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Immunology 2020Quote: ... Protein concentrations were measured after sonication by the bicinchoninic acid assay (Bio-Rad, Hercules, CA), and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343 ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration of the supernatant was measured with bicinchoninic acid assay (BCA) kit (Bio-Rad) followed by normalization of proteins via the addition of 1x passive lysis buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was extracted using the AurumTM Total RNA Fatty and Fibrous Tissue (BioRad®), which included a DNase I incubation step ...
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bioRxiv - Biochemistry 2021Quote: ... The recovery of tag-less MlaC was quantified using the ratio standard curve method (41) while that of His-tagged nanodisc-embedded complexes were quantified using the Lowry assay (DC Protein Assay, Bio-Rad). For retrograde assay ...
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bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2020Quote: ... using a DNA-binding fluorescent dye (BioRad, SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody binding was visualized using chemiluminescence (BioRad) on a BioRad ChemiDocTM Imaging System.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...