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Citations for Bio-Rad :
1 - 23 of 23 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Toxin concentration was determined using Bradford Reagent (BioRad) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 7ng of synthesized mutant DNA (designed through Bio-Rad Laboratories and ordered through Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: ... delemar pyrF mutant (strain M16)55 using the biolistic delivery system (BioRad), and the homogenous transformants were selected on minimal medium lacking uracil11 ...
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bioRxiv - Plant Biology 2021Quote: ... ORCA3 protein mutants were constructed by site-directed mutagenesis using iProofTM (Bio-Rad) and pDONR207_CrORCA3 as a template and complementary primers ...
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bioRxiv - Immunology 2022Quote: ... PR3 mutants were loaded (1 μg/lane) onto 12% Tris-HCl gels from BioRad (Hercules, CA). Protein samples were not reduced ...
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bioRxiv - Immunology 2019Quote: ... purified PR3 mutant proteins were loaded (1 µg/lane) onto 12% Tris-HCl gels from BioRad (Hercules, CA). The SDS gel electrophoresis was performed at 180 volts for 35 minutes ...
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bioRxiv - Genetics 2020Quote: ... Other cancer associated Mtg16 point mutants (http://www.cbioportal.org/public-portal/) were generated by site-directed mutagenesis using iProof DNA polymerase (Bio-Rad). The oligonucleotides used for mutagenesis are listed in Table S2.
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bioRxiv - Microbiology 2022Quote: OD600-normalized whole-cell lysates of wild-type strains (43816 and KR174) and subsequent mutants (Δcps and ΔwecA) were separated on 7.5% Mini-PROTEAN precast polyacrylamide gels (Biorad). Samples were transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... MDA-MB-231 cells were transfected with the CapG-GFP construct and its mutants using Transfectin (Biorad, Hercules, CA) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... (S10 Table) was introduced in the perRB mutant strain by electroporation as previously described [55] using a Gene Pulser Xcell (Biorad). Individual spectinomycin- resistant colonies were selected on EMJH plates containing 50 µg/ml spectinomycin and screened by PCR (using the P1 and P2 primer set ...
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bioRxiv - Systems Biology 2020Quote: ... Mutant pools were generated by electroporation of 107 haploid ESCs using a GenePulser Xcell (270 V, 500 μF, ∞ Ω, Biorad) with 0.5 μg genetrap plasmid and 10 μg hyperactive transposase (hyPBase ...
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bioRxiv - Immunology 2021Quote: ... 1μg of each protein (wild type, single domain, and domain deletion mutant) was immobilized onto NC membranes (0.2μ pore size; Bio-Rad). The membranes were kept for drying for 5-10 min at RT ...
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bioRxiv - Biophysics 2021Quote: ... 5 µL of purified (mutant) protein sample was mixed with 1.67 µL of 4X Laemmli loading buffer (Bio-Rad 1610747), then 5 µL was loaded onto an SDS-PAGE gel (Either 12% isocratic (Bio-Rad 4561046 ...
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bioRxiv - Microbiology 2020Quote: ... 10 U of HindIII restriction enzyme and 1.1 μL of a 20X primers-probes mix (ddPCR mutant assay) provided by Bio-Rad (concentrations and sequences of probes are not provided by the manufacturer but they guaranty the validation by Minimum Information for Publication of Quantitative Real-Time PCR Experiments [MIQE] guidelines) ...
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bioRxiv - Systems Biology 2021Quote: ... and 6 μl for slow growing mutants with lower OD600) on Stain-Free gels (4-20%, CAT # 4568096, Bio-Rad, Tris/Glycine SDS Buffer (CAT # 161-0732 ...
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bioRxiv - Genomics 2019Quote: ... For KRAS (G13D) and AKT1 (E17K) mutant and wild type PrimePCR™ ddPCR™ Mutation Assays were obtained from Bio-Rad (dHsaCP2000013 and dHsaCP2000014 ...
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bioRxiv - Microbiology 2019Quote: ... Expression of AcrIIA1 mutants was confirmed by harvesting 1 OD600 unit of cells and resuspending in 200 µl 1X Laemmli Sample Buffer (Bio-Rad) followed by SDS-PAGE and immunoblotting as described above ...
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bioRxiv - Genomics 2020Quote: ... Mutant and wild-type sequences were read using a droplet reader with a two-color fluorescein/HEX fluorescence detector (Bio-Rad). The mutant allele frequency was calculated as the fraction of mutant-positive droplets divided by total droplets containing a target ...
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bioRxiv - Biochemistry 2023Quote: ... for 2 h (for the ERGIC-53 ΔΗ34 mutant) The beads were transferred into a gravity flow column (Econo-column, Bio-Rad) and washed with 20 CV of buffer A supplemented with 0.02% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... the numbers of wild-type and mutant copies per 20 μL were counted using the QX200 Droplet Digital PCR System (Bio-Rad Laboratories). The mutant allele frequency (MAF ...
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bioRxiv - Plant Biology 2020Quote: Temperature sensitive mutant cells were transformed with the linearized plasmid by electroporation (500 V, 50 μF, 800□Ω) using GenePulser Xcell (Bio-Rad, CA). The cells were recovered in TAP containing 40□mM sucrose ...
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bioRxiv - Genetics 2021Quote: The relative transcription levels of nAChRs in different KO mutants were examined using real-time quantitative PCR performed with an CFX96TM Real-Time PCR System (Bio-rad, Hercules, USA). Total RNA was isolated with Trizol reagent according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... of the IDH1 R132H mutation were determined in 69 tumor sections from case one and the patient’s blood using the PrimePCR IDH1 R132H mutant assay and the QX100 Droplet Digital PCR system (Bio-Rad Inc., Hercules, CA). An initial serial dilution of a positive control was performed to optimize the input concentration of genomic DNA from each section and to assess the reliability of the assay ...