Labshake search
Citations for Bio-Rad :
1 - 50 of 1033 citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Oligomerization state of the functional bacterial twin arginine translocation (Tat) receptor complexbioRxiv - Biophysics 2021Quote: Concentrations of purified proteins were determined by the densitometry of bands on Coomassie Blue R-250 stained SDS-PAGE gels using carbonic anhydrase as a standard and a ChemiDoc MP imaging system (Bio-Rad Laboratories). Fluorescent proteins were detected by direct in-gel fluorescence imaging using the same ChemiDoc imaging system ...
-
bioRxiv - Biochemistry 2021Quote: Protein concentrations were determined by the densitometry of bands on SDS-PAGE gels stained with Coomassie Blue R-250 using carbonic anhydrase as a standard and a ChemiDoc MP imaging system (Bio-Rad Laboratories). Fluorescent proteins were detected by direct in-gel fluorescence imaging using the same ChemiDoc imaging system ...
-
bioRxiv - Microbiology 2022Quote: ... mouse-anti His (BIO-RAD; MCA1396GA), mouse-anti p65 (F-6 ...
-
bioRxiv - Microbiology 2023Quote: ... A mouse anti-His antibody (Biorad) was used as a primary antibody in a 1:500 dilution ...
-
bioRxiv - Microbiology 2022Quote: ... mouse monoclonal His tag antibody (MCA1396GA, Bio-Rad, 1:1000), mouse 2A antibody (NBP2-59627 ...
-
bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2023Quote: ... followed by incubation with mouse anti-His (1:1000, BioRad MCA1396GA) overnight at 4 °C and secondary antibody detection with IRDye800CW anti-mouse (1:5000 ...
-
bioRxiv - Immunology 2024Quote: ... The membrane was then incubated with mouse anti-His antibody (catalog number #6200203, Bio-Rad), diluted 1:1,000 in the blocking solution and incubated overnight at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... The plates were washed and incubated with mouse anti-His (Bio-Rad MCA-1396; 1:1000) followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (MerckMillipore AP124P ...
-
bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
-
bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
-
bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
-
bioRxiv - Microbiology 2021Quote: ... were treated with 20% blocking buffer (Li-Cor Biosciences, Lincoln, US) followed by incubation with anti-His-tag mouse primary antibody (dilution1/5000, Bio-Rad) and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000 ...
-
bioRxiv - Microbiology 2021Quote: ... and then incubated with a secondary goat anti-rabbit (for the anti-YjbI antiserum) or goat anti–mouse (for the anti–His-tag antibody) antibody conjugated with horseradish peroxidase (Bio-Rad) for 2 h ...
-
bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
-
bioRxiv - Cell Biology 2019Quote: ... Gradient mini-protean gels 8%-14% (Bio-Rad) were used for the western blots ...
-
bioRxiv - Bioengineering 2023Quote: ... 14% Acrylamide/Bis Solution 19:1 (Bio-Rad, cat# 1610144), 2.8 g Urea (BioShop ...
-
bioRxiv - Plant Biology 2020Quote: ... 12.5 μL of SensiMix SYBR Hi-ROX (BIO-RAD) and DNAse/RNAse free water up to 15 μL ...
-
bioRxiv - Microbiology 2023Quote: ... and an anti-His-HRP conjugate antibody (Bio-Rad) was used in a 1:4000 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
-
bioRxiv - Cell Biology 2023Quote: HEK 293T cells were transfected by electroporation using the Gene Pulser Xcell (Bio-Rad) or by lipofection with Lipofectamine 2000 (Life Technologies) ...
-
bioRxiv - Microbiology 2022Quote: ... was packaged into a 1.5×14 cm column (Bio-Rad, Carlsbad, CA) and washed with 3 column volumes of molecular-grade H2O and 6 column volumes of binding buffer + denaturing agent ...
-
bioRxiv - Microbiology 2024Quote: ... PorH-His immunoblots were performed on 16.5% tris-tricine gels (BioRad) using anti-His (mouse ...
-
bioRxiv - Developmental Biology 2020Quote: ... Proteins were separated by 14% SDS-PAGE and transferred to PVDF membranes (Biorad). Following blocking in 5% Milk (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2011) and run at 14 °C in a CHEF DR-III apparatus (BioRad) using the field 6V/cm at a 120° angle ...
-
bioRxiv - Plant Biology 2022Quote: ... Imaging was done using Hi-sensitivity chemiluminescence settings under ChemiDoc XRS+ (BioRad).
-
bioRxiv - Cancer Biology 2021Quote: ... Electrophoresis was performed at 14 °C in 0.9% pulse field-certified agarose (Bio-Rad) using Tris-borate-EDTA buffer in a Bio-Rad Chef DR III apparatus (9 h ...
-
bioRxiv - Neuroscience 2022Quote: Neuronal transfections were carried out at DIV 13–14 using Transfectin (BioRad, Hercules, USA), according to the manufacturers’ instructions (DNA:transfectin ratio 1 µg:3 µl) ...
-
bioRxiv - Genetics 2023Quote: ... Cell were counted on day 14 using a TC20 Automated Cell Counter (Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2019Quote: ... 120° included angle and 14°C constant temperature in a CHEF-MAPPER system (Bio-Rad) with pulsed times ramped from 0.2s to 120s.
-
bioRxiv - Microbiology 2022Quote: ... 120° included angle and 14 °C constant temperature in a CHEF-MAPPER system (Bio-Rad) with pulsed times ranging from 0.5 s to 3 min 10 s with a linear ramping factor.
-
bioRxiv - Microbiology 2023Quote: ... Proteins were separated on 14% Tris-Glycine gels and transferred to nitrocellulose membranes (Bio-Rad). Membranes were blocked with 3% milk in Tris-buffered saline with Tween-20 (TBS-T ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2023Quote: ... the His-FUS proteins were mixed with Profinity IMAC Ni2+-charged resin (Bio-Rad) for 30 min at 20 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... Myc-5-HT1A-R and eGFP were performed at 13–14 DIV using Transfectin (Bio-Rad), according to the instructions of the manufacturer (DNA/lipofectant ratio of 1:3) ...
-
bioRxiv - Plant Biology 2020Quote: ... The His-tagged fusion protein was purified using a Ni-NTA column (Bio-Rad, USA). Protein was eluted in elution buffer [500 mMNaCl ...
-
bioRxiv - Plant Biology 2023Quote: ... and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis, Bio-rad). Pichia transformants expressing FHS-CELLOX was already available (Scortica et al. ...
-
bioRxiv - Microbiology 2024Quote: ... Detection of His-tagged proteins was performed with the ClarityTM Western ECL substrate (Biorad, USA) and using a Versadoc (Biorad ...
-
bioRxiv - Bioengineering 2021Quote: ADMSC metabolic activity was tracked over 14 days using the alamarBlue® Cell Viability Assay (Bio-Rad). Media was removed from samples of S-50 or P-0 (n = 5 each) ...
-
bioRxiv - Systems Biology 2021Quote: ... Quantitative analyses of blotted hCDKL5 and His-Neuropinilin were carried out with Image Lab software (BioRad) and the volumetric yield was derived considering the final biomass concentration (OD600 ...
-
bioRxiv - Immunology 2023Quote: ... Colorimetric (standards) and chemiluminescent detection (His-tagged proteins) were performed using the ChemiDoc Imager (Bio-Rad).
-
bioRxiv - Molecular Biology 2021Quote: ... Electrophoresis was carried out in 14 °C cold 0.5x TBE in a CHEF DR-III system (Bio-Rad, initial switch time 60 sec ...
-
bioRxiv - Immunology 2021Quote: ... Proteins were separated on 14% polyacrylamide gels and transferred onto nitrocellulose membrane using Trans-blot Turbo (Bio-Rad). Antibodies for immunoblot were against GSDME (EPR19859 ...
-
bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
-
bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
-
bioRxiv - Microbiology 2020Quote: ... Eluents were collected and incubated with 50μL of packed Ni-NTA resin (Bio-Rad His-Pur 88222) at room temperature with nutating for 30 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... and cultures were induced with IPTG and His-MBP-ORC2 was purified on Ni-NTA beads (BioRad). Purified protein was injected into rabbits for serum generation and collection (Cocalico Biologicals) ...
-
bioRxiv - Biophysics 2022Quote: ... His-tagged σs was added to phosphorylated/unphosphorylated RssB in Micro Bio-Spin Chromatography columns (Bio-Rad) before removing 6μL of the input for SDS-PAGE analysis and adding 40μL of prepared HisPur Ni-NTA resin (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2019Quote: ... were multiplexed and diluted to a final concentration of 0.2X and amplified for 14 cycles using a PCR thermal cycler (C1000 Touch, Biorad). After amplification ...
-
bioRxiv - Microbiology 2021Quote: ... The mixtures were electrophoresed on 14% SDS PAGE and bocillin fluorescence was imaged using Gel Doc EZ imager (BioRad).