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Citations for Bio-Rad :
1 - 50 of 2366 citations for African Swine Fever Virus P30 Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... RNA was purified using Bio-Spin P30 columns (Bio-Rad). The RNAs were precipitated with 1 volume of absolute ethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... Buffer exchange was performed using P30 Micro columns (Biorad 732-6250), then treated with RQ1 DNase I and RQ1 buffer and incubated at 37°C (10min for COLO320 and 30min for GBM39) ...
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bioRxiv - Molecular Biology 2019Quote: ... Labeled samples were de-salted with a P30 column (Bio-Rad laboratories) and then purified on a 10% polyacrylamide urea gel.
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bioRxiv - Molecular Biology 2022Quote: ... The RNA was then purified using p30 size exclusion columns (Bio-Rad) and quantified ...
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bioRxiv - Biophysics 2020Quote: ... and then spun through a Micro Bio-Spin P30 Column (Bio-Rad Laboratories) to replace the solution by BRB80 buffer containing 1 mM GTP ...
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bioRxiv - Genetics 2021Quote: ... followed by clean up and buffer exchange with Bio-Spin P30 columns (Bio-rad). RNA was digested to nucleosides with 20 units of S1 Nuclease (ThermoScientific ...
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bioRxiv - Cell Biology 2019Quote: ... the desalting columns (30 kDa cut-off; Micro Bio-Spin P30 Column; Bio-Rad) were equilibrated with desalting buffer (40 mM BisTris-HCl pH 7.2 ...
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bioRxiv - Biophysics 2022Quote: ... and purified using micro Bio-spin P30 gel filtration columns (33) (Numata 2011; BioRad).
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bioRxiv - Biophysics 2020Quote: ... the sample was further spun through a Micro Bio-Spin P30 Column (Bio-Rad Laboratories) to replace GDP with GTP ...
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bioRxiv - Molecular Biology 2020Quote: ... Excessive salt and residual NTPs were removed by using P30 column (Bio-Rad, Cat# 732–6250), followed by treatment with DNase I (Promega Cat# M6101 ...
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bioRxiv - Microbiology 2021Quote: ... an assay detecting the human ribonuclease P/MRP subunit p30 RPP30 gene (Bio-Rad Assay ID dHsaCP2500350) was used as reference (probe ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were fragmented for 10 minutes on ice in 0.2 N NaOH and purified on Bio-Spin P30 columns (Bio-Rad). Biotin-labelled RNA was then purified using MyOne Streptavidin C1 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... AA-tRNAIles were purified by phenol/chloroform extraction followed by two consecutive steps on Bio-Spin P30 columns (Bio-Rad) and dialyzed against 10 mM NaOAc pH 4.5 ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μM or 3.2 μM of SAM-3H3 were used and the reaction was cleaned with either a PCR purification kit or Bio-Spin P30 columns (Bio-rad). The reactions were incubated for 2 hrs at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Removal of the residual oxidants was achieved by size-exclusion chromatography using “Micro Bio-Spin P30 Tris Chromatography Columns” (Bio-Rad, München, Germany) or Nap-5 columns (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Physiology 2020Quote: ... the trachea was isolated and mounted using insect’s needles in an extra thick blot paper (Bio-Rad) which was perfused with Ringer solution (gassed with 95%/5% O2/CO2 ...
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bioRxiv - Microbiology 2021Quote: ... and anti-canine distemper virus nucleoprotein mouse monoclonal primary antibody (Biorad) in selected fox tissues.
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bioRxiv - Neuroscience 2024Quote: ... The final virus samples were titered by digital droplet PCR (BioRad) using a REP-specific primer/probe set for libraries and a CMV primer/probe set for recombinant virus (Supplementary Table 2) ...
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bioRxiv - Biochemistry 2021Quote: ... Virus was inactivated by adding 50 μl of 2X Laemmli buffer (BioRad) and incubating at 95 °C for 10 minutes.
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bioRxiv - Microbiology 2021Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2022Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Microbiology 2021Quote: Concentrated PV or virus was mixed with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Molecular Biology 2023Quote: ... three pooled insects were homogenized with a disposable pestle in a microcentrifuge tube containing 320 µl of 2x Laemmli Sample Buffer (BioRad) and 5% of 2-mercaptoethanol ...
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bioRxiv - Immunology 2023Quote: ... (ii) genes in RNA virus infection panel of pre-designed human PrimePCR by BioRad; (iii ...
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bioRxiv - Biochemistry 2020Quote: ... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
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bioRxiv - Biochemistry 2022Quote: ... The blood was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad) before use in the mosquitoes feeding process ...
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bioRxiv - Immunology 2021Quote: ... and TaqMan Fast Virus 1-step Master Mix) using CFX-96 (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... Concentrated virus was then inactivated and proteins denatured by heating with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and subsequently run on a 4-20% Mini-PROTEAN TGX protein gel (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: Total viral RNA was extracted from 0.1 ml virus stocks using Aurum Total RNA Mini Kit (Bio-Rad) and eluted in 50 μl elution buffer pre-heated to 70°C ...
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bioRxiv - Microbiology 2021Quote: ... All serum samples were confirmed as Dengue virus-positive by means of NS1 diagnostic ELISA test (Platelia, Biorad). Serum samples were filtered using Millipore 0.22 μm PES syringe filters ...
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bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
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bioRxiv - Microbiology 2021Quote: The specific fluorescence produced during the formation of amplicons/ PCR product from the genomic RNA (converted to cDNA) of Rabies virus was identified by the detector in the qPCR machine (CFX CONNECT, BIORAD). The quantitative result is expressed in terms of relative quantification ...
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bioRxiv - Pathology 2022Quote: ... with an in-house full virus standard for determination of genome loads on a C1000 thermal cycler with the CFX96 Real-Time System (Biorad).
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bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
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bioRxiv - Microbiology 2022Quote: ... Absolute quantifications of virus RNA copies were estimated by modelling a Poisson distribution using QuantaSoft™ analysis software version 1.7 (Bio-Rad). Mean signal from N1 and N2 was extrapolated and expressed as viral RNA copies per microliter of supernatant ...
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bioRxiv - Cancer Biology 2022Quote: ... Virus quantification of KO/activation pool was done by droplet digital PCR (ddPCR) using QX200 Droplet Digital PCR System (Bio-RAD, #1864001).
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bioRxiv - Immunology 2019Quote: ... The calculation of IgG antibody concentration against each individual influenza virus strain rHA was performed by Bio-Plex Manager™ 6.2 software (Bio-Rad Co., CA).
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bioRxiv - Systems Biology 2021Quote: Differential expression of ten selected transcripts in response to virus at 3-L1 stage was examined using Droplet Digital PCR (ddPCR) (Bio-Rad, Hercules, CA). Because of the difficulty in obtaining a large amount of gut RNA samples and the limited sensitivity of conventional qPCR technique to detect gene expression at a low level ...
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bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.
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bioRxiv - Neuroscience 2020Quote: ... Cells were counted with cell counter (BioRad) and 1×105 cells were seeded onto glass coverslip coated with 0.01 % P-L-ornithine and 5ug/mL mouse natural laminin in 24-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... Cell cycle of cells (> 3 × 104) were determined by HOECHST fluorescence in single cell by ZE5 cell analyzer (BioRad) and data were processed by Flowjo.
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bioRxiv - Cell Biology 2019Quote: ... cells were counted through electronic counting cells (BioRad) for the following 6 days ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... Cells were counted using a cell counter (BioRad), collected by centrifugation at 1000rpm for 4 min ...
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bioRxiv - Genetics 2021Quote: ... pretreated plates following cell counting for viable cells using TC10™automated cell counter (Biorad) with trypan blue ...
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bioRxiv - Cell Biology 2023Quote: ... and cell density (cells/ml) was measured by an automated cell counter (TC20, Bio-Rad). The culture was diluted by medium lacking nitrogen and carbon sources to a density of 1×106 cells/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were sorted by S3 Cell Sorter (Bio-Rad). After sorting ...
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bioRxiv - Cell Biology 2020Quote: ... cells were counted using an automated cell counter (BioRad) and equal number of cells were plated in a 96 well plate ...
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bioRxiv - Genetics 2021Quote: ... Cells were counted using an automated cell counter (BioRad), and 100 000 cells per antibody were transferred to a fresh 2 mL tube ...