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Citations for Bio-Rad :
401 - 450 of 742 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... transferred to low fluorescence PVDF membranes (45 μm) with the Trans-Blot Turbo System (BioRad). Membranes were blocked with 5% powdered milk (BSA for phospho-proteins ...
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bioRxiv - Cancer Biology 2022Quote: ... The PAGE gels were transferred to precut low fluorescence polyvinylidene difluoride (PVDF) membranes (BioRad, #1620261). The membranes were blocked with 5% non-fat dry milk in Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred onto Immun-Blot Low Fluorescence PVDF membrane (Bio-Rad, Cat. no. 1620264) using Trans-blot Turbo semi-dry transfer system ...
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bioRxiv - Neuroscience 2023Quote: ... brains were rinsed and embedded in a solution of 4% low- melting agarose (Bio-Rad) and cut into coronal sections (60 µm-thick for E18.5 and P2 mice ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified over the corresponding GST-LRRCC1 fusion bound to Affi-Gel 10 resin (Bio-Rad). Other primary and secondary antibodies used in this study are listed in Table 1.
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bioRxiv - Biophysics 2021Quote: ... NRG1-Ig was purified under denaturing conditions via immobilized metal affinity chromatography (IMAC) using NGC system (Bio-Rad) equipped with a 10 mL Co-NTA column ...
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bioRxiv - Microbiology 2020Quote: ... MBP-MogR proteins were purified by amylose affinity chromatography (Poly-Prep Chromatography column, 0.8 × 4 cm, Bio-Rad). The identity and purity of proteins was confirmed by SDS-PAGE (Coomassie stain ...
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bioRxiv - Cell Biology 2024Quote: ... Antibodies were purified from rabbit serum by affinity chromatography using Affi-Prep protein A resin cartridge (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... The protein was purified from the soluble fraction by sequential affinity purification in gravity flow columns (Bio-Rad) using His60 Ni Superflow Resin (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Eluents were collected and incubated with 50μL of packed Ni-NTA resin (Bio-Rad His-Pur 88222) at room temperature with nutating for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and cultures were induced with IPTG and His-MBP-ORC2 was purified on Ni-NTA beads (BioRad). Purified protein was injected into rabbits for serum generation and collection (Cocalico Biologicals) ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins (10 μg) were applied to 4-20% gradient polyacrylamide gels (Bio-Rad) after heating them for 20 minutes at 95°C in 2x Laemmli buffer with 2% β-mercaptoethanol (BME) ...
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bioRxiv - Developmental Biology 2024Quote: The concentration of recombinant proteins was determined through a Bradford Assay (Bio-Rad, #5000006) using a NanoDrop One Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant protein was purified using the Ni-NTA (Biorad IMAC Ni-Charged Resins) affinity chromatography under denaturing conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Final recombinant protein concentrations were determined using Quick Start Bradford Protein Assays (Bio-Rad) with bovine serum albumin as the reference protein for standard curves ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The proteolytic fragments were separated on a 4-15% SDS polyacrylamide gel (BioRad) and visualized by Coomassie Blue staining.
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bioRxiv - Molecular Biology 2024Quote: ... Fragments were size selected on a 15% TBE-urea PAGE gel (Bio-Rad) with 15 and 36 nt oligos as size markers ...
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bioRxiv - Cell Biology 2020Quote: ... and rat an α-tubulin (1:1000; MCA78G; Bio-Rad AbD Serotec), polyclonal rabbit lamin B1 (1:300 ...
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bioRxiv - Immunology 2021Quote: ... slides were incubated in PBS with rat anti-CD8 (Bio-Rad, YTC182.20) plus either rabbit polyclonal anti-SARS-CoV-2 Spike/RBD (Sino ...
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bioRxiv - Pathology 2022Quote: ... and rat monoclonal anti-F4/80 antibody (Bio-Rad Laboratories, Inc., MCA497GA). Vectastain ABC kit (Vector Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... and Tub1 (rat monoclonal anti-tubulin alpha, (Bio-Rad Cat# MCA77G, RRID:AB_325003)) (1:10000 dilution each in PBS-T) ...
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bioRxiv - Neuroscience 2021Quote: ... the rat anti-mouse anti-IFN-γ antibody (Bio-Rad, Oxford, UK) and tyrphostin A47 (a tyrosine protein kinase [TPK] specific blocker ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti-Myelin Basic Protein (BIO-RAD, reference MCA409S, 1/1000 dilution), rabbit anti-β-Tubulin III (Tuj1 ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-CD68 (1:2,000, Bio-Rad, Hercules, CA; catalog no. MCA1957), rat anti-Lamp1 (1:4,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Following primary antibodies were used: anti-CD68 (rat 1:600, BioRad, MCA1957T), anti-Galactin1 (rabbit 1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... Rat anti-F4/80 (Bio-Rad, clone CI:A3-1, #MCA497GA, 1:50), Rabbit anti-CD45 (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... and a rat anti-F4/80 antibody (1/300, Bio-Rad, MCA497) to check astrocytic and microglial populations ...
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bioRxiv - Cell Biology 2024Quote: ... and rat anti-α tubulin (YL1/2) (MCA77G, Bio-Rad; 1:1500). Secondary antibodies used were Alexa Fluor 488 goat anti-mouse IgG (H + L ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were labeled with rat anti-CD68 (1:400, Bio-Rad MCA1957) or rat anti-MBP (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... rat monoclonal anti CD68 (clone FA-11, 1.4 ug/ml, Bio-Rad), rabbit polyclonal anti Iba-1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... and a rat anti-CD68 antibody (1:100; Bio-Rad, Cat #MCA1957) overnight at 4°C followed by corresponding Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... QRT-PCR reactions were carried out with SsoFastTM EvaGreen® Supermix with Low ROX (Biorad, USA) in the LightCycler® 96 kit (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... a base layer was formed by mixing 1.5% soft agar (low-melting point agarose, Bio-Rad) and culture medium at a 1:1 ratio and placing the mixture in 6-well plates ...
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bioRxiv - Genetics 2024Quote: ... 4 mg/ml lyticase) and blended with 300 μl of 2% low-melt agarose (Bio-Rad). Then ...
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bioRxiv - Biochemistry 2021Quote: ... and the proteins were purified using a Profinia immobilized metal affinity chromatography (IMAC) column with Ni2+ beads (Bio-Rad) and a Profinia protein purification system (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: Streptavidin and anti-GFP beads after affinity purification were resuspended in 15 μl of 4× Laemmli sample buffer (Biorad), boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad) ...
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bioRxiv - Bioengineering 2024Quote: ... The clarified lysate was then injected into a prepacked Ni-NTA affinity column (EconoFit Nuvia IMAC Column, Biorad #12009287) in a FPLC (NGC Quest Plus ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and antimouse secondary antibody (Biorad; 1:10,000). GroEL was used as cytoplasmic control and detected as above ...
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bioRxiv - Plant Biology 2024Quote: ... in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad) and the ChemiDoc Touch imaging system (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... the membranes were incubated with fluorescent tagged secondary antibodies for 45 minutes at RT followed by imaging on a fluorescence scanner imaging system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
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bioRxiv - Biochemistry 2022Quote: ... and eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 3 μL of the resuspended membrane pellets containing the receptors were run alongside membrane pellet of uninjected oocyte on 4–15% precast gradient TGX Stain-Free™ gels (Bio-Rad Laboratories) for 34 min at 200 V ...
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bioRxiv - Neuroscience 2020Quote: ... The amount of surface expressed receptors was detected by adding 60 µL wash buffer and 20 µL HRP substrate (Bio-Rad, 170-5060) per well ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-MF20 (DSHB, Cat# MF 20, dilution 1:20) rabbit polyclonal anti-Calcitonin Receptor (Bio-RAD, Cat# AHP635, dilution 1:2000), chick anti-GFP (abcam ...