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Citations for Bio-Rad :
2701 - 2750 of 3130 citations for IL 1 beta Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 5 µl HeLa lysate was preincubated with 1 µl of accessory protein for 10 min at room temperature in PCR plates (HSP9601, Bio-Rad). Reaction was started by adding 2 µl reaction mix ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with PBS-T for 5 min followed by 1 minute incubation with ECL detection reagent (Cytiva, RPN2106) and imaged with ChemiDoc MP Imaging System (Bio-Rad). Membranes were washed twice in TBS and stripped before reprobing according to stripping reagent manufacturer’s protocol (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... and incubated at 65°C for 3 mins before running 1% agarose-Etbr gel electrophoresis to detect the cleavage efficiency by ChemiDoc system (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer (HN: 5% Blotting Grade Blocker, Bio-Rad cat ...
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bioRxiv - Immunology 2022Quote: ... and primers to the editing locus producing amplicons of <500bp (1× assay, 900-nM primers and 250-nM probe) using ddPCR supermix for probes (no deoxyuridine triphosphate [dUTP]) (Bio-Rad). Reference reactions were simultaneously performed using a 5’ HEX labeled probe/ primer combination targeting the houskeeping gene B2M ...
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bioRxiv - Plant Biology 2022Quote: ... SybrGreen real-time quantitative PCR experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad). Data were analyzed with the comparative 2ΔΔCt method using the geometric mean of Ef1α (5’-CTG GAG GTT TTG AGG CTG GTA T-3’ and 5’-CCA AGG GTG AAA GCA AGA AGA-3’ ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse secondary antibodies (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed six times with PBS-T and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) (1:2000) (Bio-Rad) diluted in 2% horse serum/PBS for 1 hr at room temperature with agitation ...
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bioRxiv - Microbiology 2022Quote: ... and the RNA profile was measured by absorbance (254 nm) across the gradient in real time using an EM-1 Econo UV Monitor (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... and probed in triplicate by qPCR for the presence of the 1 kb capsule fragment using iTaq DNA polymerase (BIO-RAD) and primer pair DDL34-DDL35 ...
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bioRxiv - Microbiology 2022Quote: ... the generated cDNA was diluted (1:20) and amplified using stat1a- or stat1b-specific primers and 2x SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) in Hardshell® Low Profile Thin-wall 96-well skirted PCR plates (Bio-Rad ...
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bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were subsequently centrifuged at 1200 rpm for 5 minutes before their pellets being resuspended in 1 ml medium for cell counting using TC10 automated cell counter (BioRad USA) before re-plating ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA (1 μg) was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170±8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μL re-suspended Fraction B pellets were run 1 cm onto an Any kD Mini-Protean TGX gel (Bio-Rad) followed by Coomassie Blue staining ...
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bioRxiv - Microbiology 2024Quote: ... using a Superdex 30 Increase 10/300 GL (Cytiva) equilibrated with 50 mL water operated at 500 µL min−1 on an NGC medium-pressure chromatography system (Bio-Rad). Per chromatography ...
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bioRxiv - Neuroscience 2022Quote: ... First-strand cDNA synthesis was performed from 1 μg of RNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad). Primers were designed using the NCBI Primer-BLAST tool and supplied by Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted 1:50 in water and subjected to RT-qPCR using the CFX384 Touch Real-Time PCR Detection System (BIO-RAD) and the ORA-qPCR Green ROX L 2X Mix (HighQu ...
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bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated with anti-mouse or –rabbit secondary antibodies tagged with horseradish peroxidase (1/10 000, Bio-Rad Laboratories) for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent ...
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bioRxiv - Immunology 2022Quote: ... prior to overnight incubation in the same buffer with 1:2000 anti-FLAG-HRP (clone D6W5B, CellSignallingTechnology #86861S) or anti-Strep (clone Strep-tag II StrepMAB-Classic, Biorad #MCA2489P) or goat anti-mouse Igκ-HRP (SouthernBiotech #1050-05) ...
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bioRxiv - Immunology 2022Quote: ... Equal volumes of diluted (1:8) cDNA were loaded for qPCR analysis along with the SsoAdvanced Universal SYBR Green Supermix (BioRad #1725272) and 0.5 μM forward and reverse primers for target genes (listed below) ...
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bioRxiv - Zoology 2022Quote: ... RNA libraries were diluted to 20 ng μL−1 and reverse transcription was performed with an iScript cDNA synthesis kit (Bio-Rad). Primers for five genes—Clock ...
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bioRxiv - Plant Biology 2022Quote: ... The dicing reactions were performed at 37 °C for 30 min and stopped by adding 1 volume TBE-Urea sample buffer (Bio-Rad), heating at 95 °C for 10 min and quickly chilling on ice for 2 min ...
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bioRxiv - Neuroscience 2022Quote: ... Single cell suspension was stored on ice in 1X EBSS+1%BSA while the live cell count/sample was determined using a TC20 Automated Cell Counter (Bio-Rad). For the extraction of metabolites 1x10^6 cells/sample were transferred into a 1.5ml Eppendorf tube ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µL of each sucrose gradient fraction was incubated at 95 ºC with 1 % sodium dodecyl sulfate (SDS) and applied for SDS-PAGE with a 4-20 % gradient SDS-PAGE gel (Bio-rad). The proteins were transferred to a nitrocellulose membrane (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... and 1-1.5 µg RNA was used a template to make cDNA with the iScriptTM cDNA synthesis kit (Bio-rad #1708890), as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Rabbit RyR1 was purified following a previously described procedure 55 and reconstituted into amphipol A8-35 by mixing at 1:10 (w/w) with RyR1 followed by overnight detergent removal with BioBeads (Bio-Rad). For the Nb selection ...
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bioRxiv - Biophysics 2024Quote: ... containing 20 mM MgCl2 and exposed to a thermal annealing ramp (65 °C, 15’; 58-53 °C, 1 h/°C; stored at 20 °C) in a Tetrad2 (Bio-Rad) thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were washed thrice and incubated with a secondary antibody (Goat anti-Mouse IgG-HRP, 1706516, Bio-Rad Laboratories, 1:10K), also diluted in 2.5% milk in TBST ...
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bioRxiv - Bioengineering 2023Quote: ... For detection, we used an anti-rabbit HRP-coupled secondary antibody (1:5000, Cell Signalling) and Western ECL substrate (#1705061, Bio-Rad. As a loading control ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of total RNA was used in a reverse transcription reaction with the iScript cDNA Synthesis kit (Bio-Rad, 1708891) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... then incubated with ProSignal Pico Spray chemiluminescence substrate (Prometheus) for 1-3 minutes at room temperature and imaged using a ChemiDoc Imaging System (Bio-Rad). Contrast was occasionally adjusted to improve visualization of bands ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial cells were physically disrupted by adding 500 µL of 25:24:1 solution of phenol:chloroform:isoamyl alcohol and homogenizing in a bead beater (TeSeE Precess 48 Homogenizer, Bio-Rad, Hercules, CA) for 90 sec at 5300 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4 °C and subsequently washed in TBST and incubated for 1 hours at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... EtOH (p.a.)) and the Bio-Rad trans-blot® TurboTM Trans System (25 V, 0.5-1 A, 25 min; Bio-Rad, Germany). Afterward ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... All the reactants were run on 4.5% non-denaturing PAGE in 1× TBE and then Cy3 or Cy5 fluorescence signal was imaged by ChemiDoc MP imaging system (Bio-Rad). S8 Table presents details of the oligonucleotide sequence used for EMSA.
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bioRxiv - Pathology 2023Quote: ... Signal was visualized using a secondary horseradish-peroxidase-labeled goat anti-mouse antibody (goat anti-mouse IgG-HRP 1:5000, SantaCruzBiotechnology) and enhanced chemiluminescence (ECL Clarity Bio-Rad). The purity as well as equal loading (40γ ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: ... the membrane was washed with 1X PBS and thereafter incubated with HRP-conjugated secondary antibody for 1 hour at room temperature and detected using ECL reagent (BioRad, USA) under chemiluminescence ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then separated by 20% Urea-PAGE gel at 250 V for 1 h and the fluorescent signal was captured by the ChemiDoc System (Bio-Rad). For protein detection ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Cell Biology 2024Quote: Proteins were wet-transferred to polyvinylidene difluoride (PVDF) membranes at 100 V for 1 h using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked with either 5% milk (CD63 detection ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed then incubated for 1 hour at room temperature with secondary antibody (Goat Anti-Mouse Starbright Blue 520 and Goat Anti-Rabbit Starbright Blue 700, each at 1:2500, Bio-Rad). Blots were analyzed (ChemiDocTM MP Imaging System ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run at 135V on a 1% agarose gel containing ethidium bromide and bands were observed using a ChemiDoc XRS+ (Bio-Rad). Sequencing of PCR products was performed by Psomagen and resulting sequencing files were analyzed with Geneious software using NCBI gene data.
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total RNA was used for reverse transcription with the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR analysis was performed using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total RNA was used for reverse transcription with the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR analysis was performed using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with primary antibodies at 4°C overnight and subsequently incubated with HRP-conjugated secondary antibodies at room temperature for 1 h and all images were acquired using a ChemiDoc XRS+ system (Bio-Rad). Mouse anti-GAPDH antibody was used at a 1:2000 dilution with TBST containing 3% BSA (Sigma) ...