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Citations for Bio-Rad :
2651 - 2700 of 3130 citations for IL 1 beta Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Expression of AcrIIA1 mutants was confirmed by harvesting 1 OD600 unit of cells and resuspending in 200 µl 1X Laemmli Sample Buffer (Bio-Rad) followed by SDS-PAGE and immunoblotting as described above ...
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bioRxiv - Microbiology 2020Quote: The quality of genomic DNA and the PCR products were checked on 1% (w/v) agarose gel made by dissolving 1.5 g of agarose (Bio-Rad, SA) in 150 ml of 1X Tris-acetate-ethylenediaminetetraacetate (TAE ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were identified via 1% agarose gel electrophoresis and visualized in a Gel Doc system (BioRad, Hercules, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... A total of 100μL competent cells were incubated in a 1mm cuvette with 1–8μg DNA for 20 min on ice prior to pulsing in a GenePulser Xcell™ electroporator (Bio-Rad) with time constant and voltage settings at 5 ms and 1200V ...
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bioRxiv - Genetics 2020Quote: ... After four washing steps with PBST the respective secondary antibodies were added for 1 h at RT: goat anti-mouse-HRP conjugate (Bio-Rad) at 1:3000 ...
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bioRxiv - Immunology 2020Quote: ... first in 10% red blood cell (RBC) lysis buffer (Serotec, UK) for PBMCs or 1× Erythrolyse RBC Lysing Buffer (Biorad, UK) for whole blood ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was generated using 1 µg total RNA and random hexamers with SuperScriptTM III Reverse Transcriptase and qPCR performed with iQTM Supermix (Bio-Rad) using a CFX96 Real-Time Thermocycler (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... The indexed amplicons were mixed based in relative fluorescence units and run on a 1% agarose gel with Sybr Safe and gel extracted using a freeze and squeeze column (Bio-Rad). The product was quantified using KAPA Library Quant kit (KAPA Biosystems).
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bioRxiv - Genetics 2020Quote: ... membranes were washed in × 1 Tris-buffered saline-Tween (TSBT) and then blocked for ~10 min in 5% blocking buffer (Bio-Rad) in 1 × TBST ...
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bioRxiv - Genetics 2020Quote: ... After washing and incubation with a secondary antibody and 1 × TBST washes the membrane was coated with chemiluminescent substrate (Clarity Max ECL substrate, Bio-Rad) and imaged on Chemidoc MP (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... 1 μL diluted cDNA (5 ng) in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each experimental sample (four source colonies ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was diluted in water in a 1:10 ratio and 1µl was used for RT-PCR was performed with an iCycler (Biorad, www.bio-rad.com/) using a Green MasterMix (Jena Bioscience ...
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bioRxiv - Physiology 2021Quote: ... cDNA was diluted 1:5 and then 2μl was added to 5ul SYBR green PCR mixture (BioRad, 1725271, Hercules, CA, USA), 2.4ul water and 1.25 pmol primer mix ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Biochemistry 2021Quote: ... both used at a concentration of 1:200 were followed by a one-hour incubation with secondary Goat anti-(Rabbit IgG-HRP conjugate) (#1706515, Bio-Rad) at a concentration of 1:8000 at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μL of precleared samples were added to 12 μL of beads in PBS-casein (PBS containing 1% casein, Bio-rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... and increasing concentrations of NONO(53-312) were prepared and incubated at 4°C for 1 hour before loaded onto a Dot Blot apparatus (Bio-rad) containing a nitrocellulose membrane (top ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the iTaq Univer SYBR Green 1-Step Kit (Bio-Rad) on a StepOnePlus Real-time PCR system (Applied BioSystems ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA was diluted 1:20 prior to detection of GADD34 pre-mRNA transcripts using iTaq Universal SYBR Green (Bio-Rad). In short ...
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bioRxiv - Bioengineering 2021Quote: ... Acrylamide/bis solution (30%) 29:1 and natural polypeptide sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) standard were purchased from Bio-Rad. Agarose was purchased from Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... the integrity of bacmid DNA was analyzed by digestion with restriction enzyme XhoI and separation in 1% PFGE agarose (Bio-Rad) gels and 0.5×TBE buffer by pulsed-field gel electrophoresis at 6 V/cm ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were washed five times with TBST and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (adsorbed with mouse and bovine to prevent cross-reactivity, 1:10,000, #STAR124P, Bio-Rad Laboratories) in TBST for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... VNR was detected on cells fixed in cold methanol by CD51/61 immunocytochemistry (clone 23C6, 1:400; Bio-Rad, Oxford, UK). Resorption tracks on dentine discs were visualised by staining with 0.5% toluidine blue under reflected light ...
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bioRxiv - Cell Biology 2021Quote: ... but we have observed better suppression of autofluorescence at 1%.) Cells were further quenched and washed with 50 mM glycine (Bio-Rad) in DPBS (DPBS-G ...
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bioRxiv - Microbiology 2021Quote: ... incubated with specific primary antibodies for 1 h and detected with horseradish peroxidase (HRP)-conjugated secondary antibodies by using ClarityTM Western ECL Blotting Substrate (Bio-Rad). For pulldown assays between cell extract and purified Nyx and MAG mutants ...
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bioRxiv - Microbiology 2020Quote: The supernatants from the different lysis buffer extractions described above were diluted 1:4 in 4x Laemmli sample buffer (Bio-Rad) supplemented with beta-mercaptoethanol following manufacture’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 10.5 μl template DNA and 1 μl respective primer pairs (200 nM final concentration, supplementary table 1) were mixed and distributed into a 96-well qPCR plate (Bio-Rad). The plate was incubated 10 min at room temperature to digest the target DNA before droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Gel was stained with SYBR Gold (diluted 1:10000 in TBE buffer) for ∼30min in the dark and imaged on a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... in TBST/5% wt/vol non-fat milk (1 h, RT), washed (5x, TBST) followed by addition of ECL substrate (Bio-Rad) and imaged (ChemiDoc ...
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bioRxiv - Biochemistry 2020Quote: ... the anti-rabbit secondary antibody was incubated for 1 h and chemiluminescence was measured using a ChemiDoc XRS+ imaging system (Bio-Rad) after development in chemiluminescence reagent (Westar Nova 2011 ...
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bioRxiv - Molecular Biology 2021Quote: ... Digitalized images of the destained SDS-PAGEs were analyzed by the Quantity One 1-D Analysis Software (Bio-Rad, Berkeley, US) to measure the band densities at each lane of all biological replicates ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality and concentration of the RNA was assessed and first strand cDNA synthesised from 1 ug using the iScript Select cDNA Synthesis Kit (Bio-Rad) and a 1:1 mixture of Oligo (dT ...
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bioRxiv - Immunology 2020Quote: ... at 1:20,000 dilution in PBS with 1% (w/v) skimmed milk and 0.1% (v/v) Tween-20 or goat anti-porcine IgA HRP (Bio-Rad Antibodies) at 1:20,000 dilution in the same diluent.
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... The primary antibody was removed the next day and the membrane was incubated with secondary antibody at room temperature for 1 h. Enhanced chemiluminescence substrate (Clarity Western ECL Substrate, Catalog no. 1705060) from Bio-Rad Laboratories (Mississauga ...
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bioRxiv - Genetics 2020Quote: ... Collected protein samples were boiled with 1× Laemmli buffer before SDS-PAGE procedure with Mini-PROTEAN TGX Precast Gel (Bio-Rad) in Tris/Glycine Buffer (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were added to the control wells without cells (Lanes 1 and 12) ranging from 0.125 – 2 mg/ml (BioRad 500-0202) and treated with formalin and NaOH at the same concentrations as the cells ...
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bioRxiv - Genomics 2020Quote: ... FLAG immunoprecipitates were eluted at 95°C for 10 minutes and GFP immunoprecipitates were eluted at 60°C for 20 minutes into 1 X SDS-Page Buffer (Bio-Rad) supplemented with 5% BME ...
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bioRxiv - Microbiology 2021Quote: ... for 1 h at room temperature and subsequently probed with appropriate secondary antibodies coupled to Horseradish peroxidase for 1 h at room temperature followed by chemiluminescent detection with ECL reagent (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Each well was then coated with 150 µl of a blocking buffer consisting of PBS and 40 mg ml-1 blotting-grade blocker (Bio-Rad). The plates were incubated with the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... This solution was injected into a microfluidic drop maker (device 1) along with Biorad droplet generation oil for Evagreen (Biorad #1864005) with 0.5% v/v TEMED (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescent secondary antibodies (1:15,000) were incubated for 1 hour at room temperature prior to fluorescence detection using a ChemiDoc™ Imaging System (Bio-Rad). Quantification of band densitometry was performed using the FIJI ImageJ software (51).
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bioRxiv - Biophysics 2022Quote: ... Resulting membranes were resuspended in 100 μL solubilization buffer (1xPBS, 1% DDM, EDTA-free protease inhibitors) and total protein concentration was quantified using DC protein assay (Bio-Rad). For each condition ...
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bioRxiv - Bioengineering 2022Quote: ... ethidium bromide at a final concentration of 0.46 μg mL−1 was used and the DNA was visualized under UV light using a GelDoc XR+ system (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... RNA concentration was measured using a NanoDrop spectrophotometer and 1 µg of RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). Primers sequences were obtained from Harvard primerbank (sequences are listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1% SDS) and transferred onto nitrocellulose membranes (Protran) with a Trans-Blot SD semi-dry or wet transfer cell (Bio-Rad). Proteins of interest were probed using the appropriate primary and Horse Radish Peroxidase (HRP)-conjugated secondary antibodies (Jackson Immunoresearch) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed to cDNA using the iScript™ gDNA Clear cDNA synthesis kit (Bio-RAD). qPCR was performed with Applied Biosystems Fast SYBR Green master mix on an Applied Biosystems QuantStudio 6 Flex system ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were detected by using horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG (H+L)-HRP conjugate (1:10,000, Bio-Rad #1706516), goat anti-rabbit IgG (H+L)-HPR conjugate (1:10,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was carried out for 1 hour at 42°C using the iSCRIPT cDNA synthesis kit (Bio-Rad, Cat #1708841BUN) and 300 ng - 1 μg of RNA per reaction ...