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Citations for Bio-Rad :
2501 - 2550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... using a CFX Opus Real-Time PCR System (Bio-Rad Laboratories). Primers used for qRT-PCR are listed in Supplemental table 1.
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR reactions were carried out in a CFX Connect Real-time PCR detection system (BioRad, Hercules, California, USA) using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Genetics 2024Quote: ... ddPCR droplets were then generated following the manufacturer’s instructions (Bio-Rad): 20 μL of ddPCR reaction ...
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bioRxiv - Genetics 2024Quote: ... Analysis of droplet samples was then performed using the QX200 Droplet Digital PCR System (Bio-Rad). We next divided the copies/μL for HDR (%) ...
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bioRxiv - Genetics 2024Quote: ... Thermocycler (Bio-Rad) settings were as follows ...
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bioRxiv - Microbiology 2024Quote: ... Purified proteins were solubilized in Laemmli buffer (Bio-Rad) with 2% β-mercaptoethanol and heated at 95°C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... The resolved proteins were transferred to nitrocellulose membrane in Tris/glycine buffer (Bio-Rad). The membrane was blocked with 5% skim milk ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were resolved in a criterion TGX Stain-Free gel (Bio-Rad) with Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... with Tris/Glycine/SDS running buffer (Bio-Rad). The resolved proteins were transferred to nitrocellulose membrane in Tris/glycine buffer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... and then proteins were transferred to a nitrocellulose membrane using a wet Trans-Blot system (Bio-Rad, Hercules, CA, USA). The primary antibodies used were anti-slow MyHC (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... and a ChemiDoc XRS Imager System (BioRad). The target band density was identified using Image Lab 5.1 (BioRad).
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bioRxiv - Microbiology 2024Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 hr at 4°C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Developmental Biology 2024Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 (Bio-Rad), 0.01% Molecular Grade BSA (New England Biolabs) ...
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bioRxiv - Genomics 2024Quote: ... and 40 cycles on a CFX Connect light cycler (BioRad). qPCR primers are listed in Table S2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were run on 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Cat# 4561084) and proteins transferred to Immun-Blot® PVDF Membrane (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... and total protein content was quantified using DC Protein Assay (Biorad, 5000113). 15 to 30 μg of total protein lysate were resolved on SDS-PAGE gel and transferred to a nitrocellulose membrane (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... The immunoprecipitated elutions were run on precast 4-20% gradient gels (Midi Criterion™ TGX™, Biorad) and stained with Coomassie blue for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... of the IDH1 R132H mutation were determined in 69 tumor sections from case one and the patient’s blood using the PrimePCR IDH1 R132H mutant assay and the QX100 Droplet Digital PCR system (Bio-Rad Inc., Hercules, CA). An initial serial dilution of a positive control was performed to optimize the input concentration of genomic DNA from each section and to assess the reliability of the assay ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was quantitated by using the Bradford assay (Bio-Rad). Between 10 to 30 µg of protein were prepared in Laemmli buffer boiled for 5 min and proteins size separated by SDS-PAGE using NuPAGE 10% Bis-Tris 1.5 mm gels (Life Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... REVERT total protein stain (BioRad) was used as indicated by manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... Samples (10ug) were separated on 10% SDS-PAGE gel (161-0183, BioRad), then transferred to a membrane ...
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bioRxiv - Cell Biology 2024Quote: ... qRT–PCR was performed using iTaq Universal SYBR Green SuperMix (Bio-Rad, #1725121). SYBR Green real-time PCR was performed in triplicate on the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Key Resources Table ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 1ug RNA using iScript reverse transcription kit (Bio-Rad, #1708891) and diluted 1:3 in water ...
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bioRxiv - Neuroscience 2024Quote: ... Only samples with an RNA integrity score (RIN) greater than 8.0 were selected for the preparation of cDNA libraries using iScript cDNA Synthesis Kit (BioRad). One nanogram of total RNA was amplified to several micrograms of cDNA.
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bioRxiv - Molecular Biology 2024Quote: ... Images were acquired with ChemiDoc Imaging Systems (Bio-Rad Laboratories, Inc).
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bioRxiv - Cell Biology 2024Quote: ... The cDNA obtained was analyzed by quantitative polymerase chain reaction (qPCR) using the CFX96 real-time PCR detection system (Bio-Rad Laboratories). The PCRs were performed in 20 µl reaction volumes that included 50 mM Tris–HCl (pH 8.6) ...
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bioRxiv - Cell Biology 2024Quote: ... α-goat (HRP, Bio-Rad) were used at a dilution of 1:5000.
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bioRxiv - Cell Biology 2024Quote: ... before imaging using a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... Dharmacon) were transfected at 10 nM concentration using siLentFect Lipid Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and retrotranscribed with iScript Supermix (Bio-Rad Laboratories) according to manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble material was separated at 13,000 rpm for 30 minutes at 4 °C and total protein concentration was measured on supernatants using Bradford reagent (Bio-Rad). Protein extracts were denatured with NuPAGE®LDS (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... SDS-PAGE and transfer were performed on 4–15%Bio-Rad Mini/Midi PROTEAN®TGX™ and Trans-Blot®Turbo™ mini/midi nitrocellulose membranes using the Trans-Blot®Turbo™ Transfer System (Bio-Rad). Nonspecific binding sites were blocked using 5% non-fat dried milk in TBS-0.1% Tween-20 (TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... The immunoblots were visualized by using the enhanced chemiluminescent WES Dura or WES Femto (Bio-Rad) using the Amersham Imager 680 ...
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bioRxiv - Cancer Biology 2024Quote: ... The immunoblots were visualized by the enhanced chemiluminescent West Dura or West Femto (Bio-Rad) using the Amersham Imager 680 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cDNA was then used to determine the expression levels of the indicated transcripts using SYBR Green Supermix (Bio-Rad) in an Applied Biosystems ViiA 7 Real-Time PCR System and the ΔΔCT method ...
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bioRxiv - Cancer Biology 2024Quote: ... complementary DNA (cDNA) synthesis was done using the iScript Advanced cDNA synthesis kit from Bio-Rad. The PCR mix was composed out of 5 ng of cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... Array-based qPCR (BioRad, myogenesis and myopathy ...
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bioRxiv - Cell Biology 2024Quote: ... TGX gels were activated via UV light application using the Trans-Blot Turbo transfer system (Bio-Rad). While blotting ...
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bioRxiv - Cell Biology 2024Quote: ... and protein concentration was determined by Bradford assay (Bio-Rad). Five µg protein was processed for LC-MS/MS analysis ...
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bioRxiv - Cell Biology 2024Quote: ... 15 µg protein were loaded onto a 4-15% Mini-PROTEAN TGX Stain-Free precast gel (Bio-Rad). The protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were blotted on low-fluorescent PVDF (Biorad) with NuPAGE transfer buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 250-1000 ng of total RNA using an iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories). Quantitative PCR for each candidate gene was performed using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoblots were developed using enhanced chemiluminescence and signals captured by exposure to film or on a CCD-camera-based digital documentation system (BioRad).
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bioRxiv - Cell Biology 2024Quote: ... The bands were visualized by enhanced chemiluminescence using Clarity (Bio-Rad #1705060) using a Bio-Rad ChemiDoc MP.
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bioRxiv - Cell Biology 2024Quote: ... on a real-time PCR detection system (CFX96; Bio-Rad, Hercules, CA). Relative mRNA levels were calculated using the ΔCt method ...
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bioRxiv - Cell Biology 2024Quote: ... and Precision Melt Supermix (Biorad). The melting curves of amplified product DNA fragments were quantified against the methylation standard curve generated using commercial bisulfite converted human genomic DNAs (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... Following blotting onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... Following pre-amplification of cDNA (SsoAdvanced PreAmp Supermix (BioRad, cat# 172-5160) for 12 cycles ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined using the Bradford assay (Bio-Rad, 5000006). SDS buffer (313 mM Tris (pH 6.8) ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... All electrophoresis supplies were purchased from Bio-Rad. All primers for cloning were purchased from Integrated DNA Technologies (IDT) ...