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Citations for Bio-Rad :
651 - 700 of 2581 citations for Anti PAK3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed three times and incubated with secondary antibodies: Donkey anti Sheep/Goat IgG (dilution 1:100,000, #STAR88P, Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated with an Alkaline Phosphatase-conjugated Anti-Digoxigenin antibody and the chemiluminescent signal was revealed using a ChemiDoc MP imaging system (Biorad).
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bioRxiv - Immunology 2023Quote: ... Blocked membranes were incubated with HuCAL anti-bovine Mincle CRD antibodies (2.5 µg mL-1) before incubation with secondary goat-anti-human IgG F(ab’)2: HRP (STAR126P, Bio-Rad) antibody ...
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bioRxiv - Immunology 2022Quote: ... Transfectants were stained for viability using a Live dead blue dye followed by anti-HLA DR DP DQ antibodies (BioRad clone Bu26 ...
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bioRxiv - Immunology 2023Quote: ... IgA and IgG antibody isotypes were detected by horse reddish peroxidase (HRP)-conjugated goat anti-pig antibodies specific for porcine IgA (clone AAI40P, BioRad) diluted 1:5 000 ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was visualized by incubating the membrane with an HRP-labelled anti-rabbit antibody followed by detection using ECL (Biorad) at the Chemidoc (Biorad).
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bioRxiv - Microbiology 2023Quote: ... Membranes were stained with primary antibody in blocking buffer at 4°C overnight: anti-NP (1:5000, OBT1555, Bio-Rad), anti-PA ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated with the following secondary antibody diluted 1/5000 in milk PBS-T: Goat anti-rabbit IgG HRP conjugate (170-6515; BioRad) or Goat anti-mouse IgG HRP conjugate (170-6516 ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were visualized using the secondary antibody Anti-Mouse IgG-HRP using Clarity Max™ Western ECL Substrate (BioRAD).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with secondary antibody goat anti-rabbit conjugated to HRP (1:5000, 5% wt/vol milk in TBS-T; #170-6515, BioRad) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Additional antibodies used for the infected ALI cultures include: mouse anti-RSV Fusion protein (1:100, Cat# MCA490, Bio-Rad), rabbit anti-Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed 3X in PBST (10 minutes) and incubated for 1 hour with horseradish peroxidase conjugated anti-rabbit secondary antibody (BioRad 170-6515 ...
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bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was incubated with 10 mL of 3% BSA/TBST (w/v) with 1 µL anti-mouse-HRP secondary antibody (1:10,000; 170-6516, Bio-Rad) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were then incubated in primary antibodies at 4°C overnight (1:500, rat anti-mouse CD68, Bio-rad Cat ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then cleared by centrifugation for 15 minutes at 14,000 rpm at 4°C and subjected to immunoprecipitation with anti-V5 mouse monoclonal antibody (#MCA1360, Bio-Rad) overnight at 4°C with rotation in DNA/RNA LoBind tubes ...
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bioRxiv - Biophysics 2019Quote: ... and horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) antibody (catalog no. 1721011, batch no. 64109318) were all purchased from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with StarBright Blue 700 Goat Anti-Mouse IgG secondary antibody (1:2500, Bio-Rad, Cat# 12004159, RRID: AB_2884948), StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Neuroscience 2021Quote: ... CD11b+ cells were used for flow cytometric analysis after cells were stained with Alexa647-conjugated rat anti-mouse CD204 antibody (clone 2F8; Bio-Rad Laboratories GmbH ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... CldU or IdU were detected by immunofluorescence with either anti-BrdU antibody that recognizes CldU (MCA2060, Bio-Rad Laboratories, Hercules, CA) or anti-BrdU antibody that recognizes IdU (347583 ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were incubated with HRP-conjugated secondary antibodies for both FLAG and GFP stainings (Rabbit anti-goat-HRP conjugate, #1721034, Bio-Rad) diluted at 1:5000 ...
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bioRxiv - Bioengineering 2019Quote: ... The anti-SSTR2 mAb was purified using our two-step antibody purification protocol by the NGC system (Bio-Rad, Hercules, CA) equipped with Protein A column and ion exchange column.(34 ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with 1:200 rabbit-anti NF-B p65 polyclonal antibody (Pierce) (2 h) and 1:5000 Goat anti-rabbit IgG (H+L)-HRP conjugate (Bio-Rad) (1 h) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (Supplementary Table 1) overnight at 4°C and visualised with HRP-conjugated anti-IgG secondary antibodies and enhanced chemiluminescence substrates (Bio-Rad). The expression of PDHX or calnexin was used as a loading control.
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bioRxiv - Molecular Biology 2020Quote: ... for 2 h at room temperature and a HRP-conjugated goat anti-rabbit secondary antibody (Bio-Rad 1706515; 1:3000 dilution) for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... or anti-PfADF1 (1:2000, (Wong et al, 2011)) and HRP-coupled goat anti-mouse or -rabbit secondary antibody (1:5000, STAR120P/STAR121P, Bio-Rad). sBlots were washed in PBST and detected using ECL reagent (Amersham ...
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bioRxiv - Microbiology 2021Quote: ... and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000, Li-Cor Biosciences) using a ChemiDoc− Touch Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... were treated with 20% blocking buffer (Li-Cor Biosciences, Lincoln, US) followed by incubation with anti-His-tag mouse primary antibody (dilution1/5000, Bio-Rad) and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000 ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated in secondary antibody solutions (TBST with 5% Non-Fat Dry Skinned Milk and Horseradish Peroxidase conjugated secondary antibodies Goat Anti-Rabbit/Mouse, 1:10000, Bio-Rad) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Bound IgG1 and IgG2 antibodies were detected by adding 100μl/well sheep anti-bovine IgG1 or IgG2 HRP (Bio-Rad, USA) at 1:5000 and 1:2500 respectively.
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bioRxiv - Biophysics 2022Quote: ... Aqueous solutions in BRB80 pH 6.9 were introduced in the chamber in the following sequence: 20 μL of 0.05 mg/mL anti-tubulin antibody (Bio-Rad Laboratories) for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were washed three times with T-TBS for 15 min and incubated with goat anti-rabbit HRP-conjugated secondary antibodies (Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Pathology 2021Quote: ... The sections were then incubated in blocking buffer (10% goat serum; 1 hour) followed by incubation with either rat anti-F4/80 antibody (Bio-Rad) diluted 1:500 or rat anti-CD68 antibody (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... were used for Western blotting together with anti-mouse IgG antibody conjugated with horseradish peroxidase (goat-polyclonal, 5178-2504, Bio-Rad). Chemicals were purchased from Sigma-Aldrich unless specified otherwise.
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bioRxiv - Molecular Biology 2019Quote: ... 80 μL of the supernatant was removed (termed ‘whole cell extract [WCE] sample’) and 5 μg of antibody (anti-PK (Bio-Rad) or anti-HA (Roche) ...
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bioRxiv - Pathology 2020Quote: ... proteins were subjected to primary antibody recognition using an anti-P1 antibody raised in rabbit (Siré et al, 2008) and purified by Affi-Gel 15 immnunoaffinity chromatography (BIO-RAD) using the recombinant P1 protein as the ligand (used at a 1:1000 dilution) ...
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bioRxiv - Neuroscience 2020Quote: ... 1:5000), or donkey anti-goat antibody (sc-2020, 1:5000) and signal was detected with enhanced chemiluminescence substrate (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... After four washing steps with PBST the respective secondary antibodies were added for 1 h at RT: goat anti-mouse-HRP conjugate (Bio-Rad) at 1:3000 ...
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bioRxiv - Neuroscience 2019Quote: ... and lysed then immunoprecipitated as described above using homemade rabbit anti-PGRN antibodies bound to Affi-Gel 15 (Bio-Rad Laboratories). Samples were then mixed and boiled 5 minutes with 10mM DTT followed by alkylation by treating samples with a final concentration of 28 mM iodoacetamide ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Biochemistry 2020Quote: ... for 1hr at RT and probed with HRP-conjugated anti-rabbit or mouse IgG antibodies and detected with ChemiDoc XRS+ imaging system (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... The membranes were washed five times with TBST and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (adsorbed with mouse and bovine to prevent cross-reactivity, 1:10,000, #STAR124P, Bio-Rad Laboratories) in TBST for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... the anti-rabbit secondary antibody was incubated for 1 h and chemiluminescence was measured using a ChemiDoc XRS+ imaging system (Bio-Rad) after development in chemiluminescence reagent (Westar Nova 2011 ...
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bioRxiv - Immunology 2020Quote: ... at 1:20,000 dilution in PBS with 1% (w/v) skimmed milk and 0.1% (v/v) Tween-20 or goat anti-porcine IgA HRP (Bio-Rad Antibodies) at 1:20,000 dilution in the same diluent.
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bioRxiv - Cell Biology 2020Quote: ... Lysates of WT OSC were incubated with 1μg of Rabbit anti-Piwi polyclonal antibody (18) and 30 μl Surebeads Protein A Magnetic beads (Bio-Rad, 1614013). Immunoprecipitation was performed at 4°C overnight ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... The membrane was incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies in 5% milk and bands were developed with Chemi-Doc XRS imaging (Bio-Rad). The primary antibodies used are listed in Supplementary Table 3.
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were detected by using horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG (H+L)-HRP conjugate (1:10,000, Bio-Rad #1706516), goat anti-rabbit IgG (H+L)-HPR conjugate (1:10,000 ...
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bioRxiv - Immunology 2022Quote: ... were coated with 50 µL/well of 2 µg/mL of mouse anti-bovine IFN-γ mAb (CC330 Bio-Rad Antibodies) diluted in carbonate coating buffer (pH 9.6 ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed for 5 min 3x with TBS-T and incubated with secondary goat anti-rabbit-HRP antibody (Bio-Rad) at a 1:10000 ...