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Citations for Bio Basic :
1 - 50 of 61 citations for Chromosome 10 Open Reading Frame 82 C10ORF82 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Knock-out cell lines were used to prepare over-expression cell lines by transfection of a pcDNA3.1(-) plasmid expressing the complete human CasD1 cDNA open reading frame synthesized by Bio Basic (Markham, Ontario, Canada). Transfected cells were selected with G418 and single cell clones screened by staining with PToV-P4 HE-Fc to identify 9-O-Ac positive cell lines ...
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bioRxiv - Biochemistry 2019Quote: ... was synthesized and cloned into a modified pEG BacMam vector 32 in frame with a C-terminal FLAG affinity tag (Bio Basic Inc.). The full length wild-type protein was expressed by baculovirus-mediated transduction of HEK293S GnTI− (N-acetylglucosaminyltransferase I-negative ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 10% dextran sulfate (Bio Basic, #DB0160), 5 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2021Quote: ... To quench the reaction 1.5 μL of 0.5 M EDTA pH 8.0 was added and the reaction subsequently heated to 75°C for 10 min before the RNA was purified via EZ-10 Spin Column RNA Cleanup and Concentration Kit (Bio Basic).
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bioRxiv - Immunology 2019Quote: ... or DNase I (10 μM, Bio basic Canada Inc.). For bactericidal activities of remnant neutrophils ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.5 μL of 10 mM dNTP (Bio Basic Inc., Canada), 0.15 μL of 5 U/μL Taq DNA polymerase (BioShop Canada Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... coli using an EZ-10 total RNA purification kit (Bio Basic) and the integrity/purity confirmed using formaldehyde agarose gel electrophoresis and A260/A280 ratio (Biodrop) ...
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bioRxiv - Immunology 2020Quote: EZ-10 Spin Column RNA Cleanup &Concentration Kit (Bio Basic Inc.);
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bioRxiv - Systems Biology 2023Quote: EZ-10 Total RNA Mini-Prep Isolation kit (Bio Basic, Canada) was used according to the kit’s instructions to extract total RNA from the heart samples ...
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bioRxiv - Immunology 2021Quote: ... and EZ-10 DNAaway RNA Mini-Preps kit (Bio Basic Inc., Markham, ON). Cells were homogenized using glass beads in 1ml TRIzol Reagent and incubated on ice for 30 minutes ...
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bioRxiv - Systems Biology 2020Quote: ... and 10 μg of RNase A (Bio Basic, Markham, ON, Canada, Cat# RB0473) and incubated on ice for 5 minutes with occasional agitation of the plate ...
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bioRxiv - Neuroscience 2020Quote: ... and EZ-10 DNAaway RNA extraction mini-prep kit (Bio Basic, Ontario, Canada) following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Phase transition was initiated by dilution in imidazole buffer (10 mM imidazole (Bio Basic) pH 7.0 ...
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bioRxiv - Genomics 2019Quote: DNA from each cell line was extracted using EZ-10 Spin Columns (Bio Basic #BS427) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 200 µl of the supernatant were transferred into EZ-10 Spin Columns (Bio Basic Inc.) and rapidly spun down (21130 g ...
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bioRxiv - Immunology 2021Quote: RNA from SFs was extracted using either EZ-10 RNA Mini-Preps Kit (Bio Basic, USA) or RNeasy Micro Kit (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using 5X All-in-One RT Master Mix (Bio Basic cat# HRT025-10) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA was extracted using the EZ-10 Spin Column Plasmid DNA Minipreps Kit (Bio Basic) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The PCR product was purified using EZ-10 Spin Column PCR Products Purification Kit (BS664, Bio Basic) and eluted in 30 µl sterile water prior to sequencing.
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using 5X All-in-One RT Master Mix Kit (Bio Basic cat# HRT025-10). Real-time quantitative PCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S ...
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bioRxiv - Molecular Biology 2020Quote: Plasmid DNA was prepared using either the EZ-10 Spin Column Plasmid DNA Miniprep Kit (Bio Basic) or the QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were purified using either the EZ-10 Spin Column PCR Products Purification Kit (Bio Basic) or the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted using the EZ-10 Spin Column Plant RNA Mini-Preps Kit (Bio Basic, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: RNA from cerebellum tissue was isolated using EZ-10 DNAaway RNA Mini-Preps Kit (Bio Basic, Canada) according to the manufacturers’ protocol ...
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bioRxiv - Microbiology 2023Quote: ... the resulting products were purified using the EZ-10 Spin Column PCR Products Purification Kit (Bio Basic) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... total RNAs were extracted with the EZ-10 DNAaway RNA Mini-Preps kit (Bio Basic, cat# BS88136) as recommended ...
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bioRxiv - Molecular Biology 2020Quote: ... each filter sample was rolled and placed in the spin column (EZ-10; Bio Basic, Markham, Ontario, Canada) with the attached silica-gel membrane ...
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bioRxiv - Immunology 2021Quote: ... Xela VS2 and EPC cells using the EZ-10 Spin Column Total RNA Minipreps Super Kit (Bio Basic) according to the manufacturer’s specifications with modifications to include an on-column DNase I digestion as described previously by our group (Bui-Marinos et al. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... whose DNA was extracted using the EZ-10 96-well Plate Animal Genomic DNA® kit (Bio Basic). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reactions were purified by EZ-10 Spin Column DNA PCR Purification Kit (Bio Basic Inc. #BS664-120712) with elution by 30 µL of ultrapure water instead of 50 µL elution buffer.
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bioRxiv - Evolutionary Biology 2021Quote: ... BHIC agar was supplemented with 10% sucrose (50% stock solution made by mixing 125g of sucrose (Bio Basic SB0498) in water to make up 250 ml and sterilized by autoclaving ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were lysed and RNA was extracted using EZ-10 DNAaway RNA Mini-Prep Kit (Bio Basic inc). cDNA was synthesized using Superscript IV (Thermo Fischer ...
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bioRxiv - Plant Biology 2021Quote: ... RNA extraction was performed using the EZ-10 Spin Column Plant RNA Miniprep Kit (Bio Basic Inc., Toronto, Canada). RNAs were reverse transcribed into cDNAs by OneScript Reverse Transcriptase (Applied Biological Materials Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were fixed with 4% PFA in PBS for 10 min, washed and resuspended in 200μl permabilization/washing buffer (PBS [-]Ca [-]Mg, 0.1% TWEEN 20 (BIO BASIC), 1% BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... Each filter disc was placed into a spin column (EZ-10 SpinColumn & Collection Tube; Bio Basic Inc., Ontario, Canada), from which a silica-gel membrane was prospectively removed ...
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bioRxiv - Molecular Biology 2023Quote: RNA was extracted from tissue culture cells using either the EZ-10 spin column kit (Bio Basic, Markham, Canada) or the MagMAX mirVana Total RNA Isolation Kit (Thermo Fisher Scientific A27828 ...
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bioRxiv - Microbiology 2021Quote: Total RNA was isolated from lysed cells using an EZ-10 spin column total RNA minipreps super kit (Bio Basic) following manufacture instructions ...
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bioRxiv - Immunology 2022Quote: ... DNA was purified using EZ-10 Spin Column Animal Genomic DNA Mini-Prep Kit (Bio Basic, Inc., Markham, Ontario, CA). Spirochete burdens were quantified based on the amplification of recA from B ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was isolated from cells after the indicated treatments using the EZ-10 DNAaway RNA Miniprep kit (Bio Basic). Reverse transcription to generate cDNAs was performed using the qScript cDNA SuperMix (Quantabio) ...
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bioRxiv - Immunology 2020Quote: ... All the mRNA products were concentrated and purified via EZ-10 Spin Column RNA Cleanup & Concentration Kit (Bio Basic Inc.). After that ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 1/10 volume of 1.5M Tris-HCl pH 8.8 (Tris Base Fisher BP152-5, Hydrochloric Acid Fisher A144-212) and 1/10 volume 1 M DTT (Bio Basic DB0058). Samples were boiled for 5 minutes before an additional centrifugation at 4 °C at 16,000 x g for 4 minutes.
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bioRxiv - Immunology 2021Quote: ... Total RNA was isolated from combined adherent and suspension cells using the EZ-10 Spin Column Total RNA Minipreps Super Kit (Bio Basic) with the following modification ...
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bioRxiv - Plant Biology 2021Quote: ... and plasmid DNA was extracted with the EZ-10 Spin Column Plasmid DNA Minipreps Kit (Bio Basic Inc., Markham, ON, Canada). The extracted destination vectors were confirmed by PCR with the primers T-35S-F (5’-AGGGTTTCTTATATGCTCAACACATG-3’ ...
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bioRxiv - Plant Biology 2021Quote: ... and plasmid DNA was extracted with the EZ-10 Spin Column Plasmid DNA Minipreps Kit (Bio Basic Inc., Markham, ON, Canada). The extracted expression vectors were amplified by PCR with the primers 35S Promoter (5’-CTATCCTTCGCAAGACCCTTC-3’ ...
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bioRxiv - Plant Biology 2021Quote: ... and plasmid DNA was extracted with the EZ-10 Spin Column Plasmid DNA Miniprep Kit (Bio Basic Inc., Markham, ON, Canada). The extracted entry vectors were confirmed by PCR with the primers M13-F (5’-GTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Microbiology 2021Quote: ... Samples were incubated for 60 min at 37°C with 10 pg µl-1 of RNase A (Bio Basic, Toronto, Canada). RNase activity was inactivated by adding 100 unites of Protector RNase Inhibitor (Roche ...
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bioRxiv - Bioengineering 2019Quote: ... Loading volumes of 10-15 μg of protein per well were calculated using a MicroBCA Protein Assay Kit (Bio Basic, SK3061) according to the manufacturer’s specification ...
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bioRxiv - Molecular Biology 2023Quote: ... and split into two tubes for either viral antigen staining or RNA extractions using the EZ-10 Spin Column Total RNA Miniprep Super Kit (Bio Basic).
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bioRxiv - Evolutionary Biology 2019Quote: ... the thoraxes of 50 to 100 representative adult flies (Table S1) were pooled for DNA extraction using the EZ-10 spin column genomic DNA minipreps kit (Bio basic inc.). Barcoded DNA PE libraries with insert size of ca ...
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bioRxiv - Plant Biology 2020Quote: ... Three leaves of four-week-old plants (~50 mg) were collected for RNA extraction by EZ-10 Spin Column Plant RNA Miniprep Kit (Bio Basic Canada). RNAs were then reverse transcribed into cDNAs by OneScript Reverse Transcriptase (Applied Biological Materials Inc.) ...