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Citations for Boston Biochem :
1 - 32 of 32 citations for Ub Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Ub-AMC (Boston Biochem) was used at a concentration (0.5 to 1 μM ...
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bioRxiv - Biochemistry 2021Quote: ... 50 µM Ub (Boston Biochem), 10 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μM Ub-VS (Boston Biochem) was added to the proteasomes binding resin and incubated for 2 h at 30 °C ...
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bioRxiv - Biochemistry 2021Quote: Ubiquitin-AMC (Ub-AMC; Boston Biochem) hydrolysis assay was used to verify the wild-type and mutant USP14 deubiquitylating activities in the human proteasome ...
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bioRxiv - Biochemistry 2020Quote: Ub-AMC (Boston Biochem, Cat. # U-550)
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bioRxiv - Microbiology 2020Quote: ... 5 µM Ub (U-100H, Boston Biochem) and 5 µM UbcH7 (E2-640 ...
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bioRxiv - Biochemistry 2021Quote: Ub-AMC (Boston Biochem, Cat. # U-550)
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bioRxiv - Microbiology 2020Quote: ... 5 µM Ub (U-100H, Boston Biochem), 5 µM UbcH7 (E2-640 ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μM HA-Ub (Boston Biochem, U-110), and 10 μM MgATP (Boston Biochem ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Lys48 linked tetra-Ub obtained from Boston Biochem was incubated at 10.5μM with 23nM SARS-CoV-2 PLpro and 2.25mM Losartan ...
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bioRxiv - Neuroscience 2024Quote: ... Non-phosphorylated Ub protein (Boston Biochem, U-100H), free non-phosphorylated ...
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bioRxiv - Neuroscience 2024Quote: ... and p-S65-Ub monomer (Boston Biochem, U102) were initially dissolved in RIPA buffer (stock concentration of 1 µg/µl) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Ub mutants K63R and K48R are from Boston Biochem.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Lys48 linked tetra-Ub were obtained from Boston Biochem; DL-dithiothroitol (DTT ...
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bioRxiv - Cell Biology 2022Quote: ... E2 (GST-UbcH5b) and HA-Ub were purchased (Boston Biochem). GFP-HP1β from transfected HEK293T cells was immunoprecipitated with the GFP-Trap and incubated at 37°C for 60 min with the complete ubiquitin reaction system consisting of reaction buffer (25 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biochemistry 2020Quote: ... Ub-Lys-TAMRA was purchased from Boston Biochem (Boston, MA). MALDI-TOF MS 1536 AnchorChip was purchased from Bruker Daltonics (Bremen ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of 10 mg/ml Ub solution (Boston Biochem; #U-100H), and 1μl radiolabeled in vitro translated protein of interest ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15−7-amino-4-methylcourmarin (ISG15-AMC ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 10 mg/ml Ubiquitin (Ub) solution (Boston Biochem; #U-100H) and 1 µl radiolabeled IVT product of interest ...
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bioRxiv - Biochemistry 2021Quote: ... a mixture of poly-Ub chains (Ub2–Ub7) (Boston Biochem #UCB-330-050), as the substrate ...
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bioRxiv - Microbiology 2023Quote: ... reaction mixtures (12.5 μl) containing 5 μg of recombinant human Ub (Boston Biochem), 80 nM recombinant human E1 (Boston Biochem) ...
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bioRxiv - Biochemistry 2021Quote: ... were charged with 10 µg ubiquitin or N-terminally fluorescein-labelled Ub (FluoUb, Boston Biochem) using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1 ...
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bioRxiv - Microbiology 2021Quote: ... with the following substitutions: USP7 assays contained 4 nM USP7 and 0.5 uM Ub-AMC (Boston Biochem); USP14 assays contained 1.7 uM USP14 ...
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bioRxiv - Cell Biology 2019Quote: ... S3 G1 extracts were mixed with i) 0.04 mg/ml Rhodamin-coupled Ubiquitin (Rd-Ub) (Boston Biochem; #U-600); ii ...
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bioRxiv - Neuroscience 2021Quote: ... 15 µl of 2x concentrated ubiquitin-rhodamine 110 (Ub-Rho110) (200 nM, final assay concentration = 100 nM; Boston Biochem), prepared in USP30 activity assay buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 20 µL of two-fold dilution series of ligand and 0.2 µM N-terminally FITC-labeled Ub (Boston Biochem) prepared in buffer were incubated in wells for 1 hour at 4⁰C ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were initiated by the addition of 250 nM ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, Boston Biochem) substrate to PfUBP-1 wt or catalytic inactive PfUBP-1 C3179A mutant ...
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bioRxiv - Biochemistry 2021Quote: ... We added 29 µM of the enzyme:peptide mixture to 1 mL of 30 µM Ub-AMC (7-amino-4- methylcoumarin) (Boston Biochem), for a final concentration of 200 nM DUBm ...
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bioRxiv - Biophysics 2024Quote: ... We added 10 μL of the enzyme:peptide mixture to 10 μL of 250 nM Ub-AMC (7-amino-4-methylcoumarin) (Boston Biochem). DUB activity was measured by monitoring the increase in AMC fluorescence in a plate reader ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant of each single B cell well was screened for antigen specificity through direct ELISA for targeting full-length monomeric p-S65-Ub protein (Boston Biochem, U-102), free p-S65-Ub peptides 1 and 2 and BSA-conjugated p-S65-Ub peptides 1 and 2 (provided by 21st Century Biochemicals) ...
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bioRxiv - Developmental Biology 2022Quote: ... through detection of the 7-amino-4-methylcoumarin (AMC) labeled fluorogenic peptide substrates Z-LLE-AMC (Boston Biochem #S-230) and LLVY-AMC (Chemicon #APT280) ...
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bioRxiv - Biochemistry 2021Quote: ... Peptidase activities of purified immunoproteasomes were assayed using fluorogenic peptide substrates Ac-ANW-AMC for PSMB8 and Ac-PAL-AMC for PSMB9 (Boston Biochem). The measured activity was normalized against total protein concentration ...