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Citations for Boston Biochem :
1 - 26 of 26 citations for Recombinant Mouse F3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 buffer (10% sucrose ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged human recombinant ubiquitin (Boston Biochem) was added to egg extracts at a final concentration of 0.1 μg/μL unless otherwise stated ...
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bioRxiv - Biochemistry 2021Quote: USP8 immunoprecipitates or His-tagged FRIP8 precipitates were incubated with 1-μM ubiquitin-VME (Boston Biochem) in TBS supplemented with 1-mM DTT at 37°C for 5 min or 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... translations were performed in the presence of 10 μM HA- or His-tagged Ubiquitin (Boston Biochem, USA). Denaturing immunoprecipitation of the ubiquitinated species was performed as previously described (Yanagitani et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged ubiquitin mutants (Boston Biochem) were added at final concentrations of 1 µg/µL ...
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bioRxiv - Plant Biology 2021Quote: ... 1.25 µg Flag tagged ubiquitin (Boston Biochem), 2 µg purified GST-PUB25 and PUB26 or HIS-PUB26 were added to 30 µL of ubiquitination reaction buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... 1 µM His-Ubiquitin (Boston Biochem, Cat#U-530), 50 nM mouse E1 enzyme ...
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bioRxiv - Cell Biology 2023Quote: ... UbcH5c and His-ubiquitin were purchased from Boston Biochem. E1 (50 nM) ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mg His-ubiquitin (BB-U-530, Boston Biochem), 2 mg purified MBP-KEG fusion protein (as E3 enzyme) ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant human UBE1 (E-305, Boston Biochem), recombinant human UbcH5a/UBE2D1 (E2-616 ...
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bioRxiv - Microbiology 2023Quote: ... 80 nM recombinant human E1 (Boston Biochem), 400 nM recombinant human E2 enzymes (Boston Biochem ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant human UbcH5a/UBE2D1 (E2-616, Boston Biochem), Myc-ubiquitin (U-115 ...
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bioRxiv - Neuroscience 2024Quote: Recombinant p-S65-PRKN (Boston Biochem, E3-166), non-phosphorylated PRKN (Boston Biochem ...
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bioRxiv - Microbiology 2023Quote: ... 400 nM recombinant human E2 enzymes (Boston Biochem) and 400 nM purified E3 ligases in 50 mM Tris-Cl (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... E3 (0.5 μM, 6 μl, His-cIAP1, Boston Biochem, E3-280) and 15 μl of purified FLAG-IL-1β in Ubiquitin assay buffer containing 2 mM ATP (total volume of 30 μl) ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... Ubiquitin (U-100H) and recombinant DUBs were provided by Boston Biochem (E-519 ...
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bioRxiv - Biochemistry 2020Quote: ... Pure recombinant human RNF114 was purchased from Boston Biochem (K-220). RNF114 (0.25 μg ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... Ubiquitin vinyl sulfone tagged with HA (U-212) and HA-ubiquitin (U-110) purchased from Boston Biochem. For immunoprecipitation anti-HA-Agarose beads (A2095 ...
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bioRxiv - Microbiology 2023Quote: ... reaction mixtures (12.5 μl) containing 5 μg of recombinant human Ub (Boston Biochem), 80 nM recombinant human E1 (Boston Biochem) ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 nM GST-tagged Ubiquitin-activating enzyme E1 and 250 nM Ubiquitin-conjugating enzyme E2 UbcH5 [both from Boston Biochem, USA]) ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
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bioRxiv - Microbiology 2021Quote: ... The SCFFBW7 E3 ligase complex was incubated with recombinant 0.1 mM E1 (UBE1; Boston Biochem), 0.25 mM E2 (cdc34 ...
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bioRxiv - Molecular Biology 2019Quote: ... UBE2TC86K was conjugated to ubiquitin through an isopeptide bond in a 200 µL reaction containing 125 µM His-Ubiquitin (Boston Biochem U-530), 125 µM UBE2TC86K ...
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bioRxiv - Microbiology 2023Quote: ... each 25 μl pelleted resin was resuspended with 4μg of recombinant human homotypic-linked polyubiquitin chains Ub3-7 (K63 or K48, 4μg/600μl reaction volume, Boston Biochem, R&D) and 500μM ε-NAD in 1X ART buffer for 2 hrs with gentle end-over-end rotation at 37°C ...
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bioRxiv - Immunology 2019Quote: ... and 1 µg of mouse E2 (UBE2E3, Boston Biochem) in 40 µl of reaction buffer (Boston Biochem ...
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bioRxiv - Immunology 2019Quote: ... 0.1 µg of mouse E1 (UBE1, Boston Biochem, Cambridge, MA, USA), and 1 µg of mouse E2 (UBE2E3 ...