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Citations for Boston Biochem :
1 - 19 of 19 citations for Proteasome Subunit Beta Type 3 PSMB3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: The proteasome inhibitor MG132 (Boston Biochem) was used in this study ...
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bioRxiv - Biochemistry 2021Quote: ... human proteasomes were treated with ubiquitin-vinyl-sulfone (UbVS, Boston Biochem). UbVS was added to the resin at 1-1.5 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was inhibited via addition of 200 μM MG262 (Boston Biochem). Egg extracts were supplemented with recombinant proteins as detailed below and incubated for 10 min at RT before addition of plasmid DNA unless otherwise stated ...
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bioRxiv - Molecular Biology 2023Quote: ... in the presence of 5 nM of 20S proteasome (Boston Biochem, E-360), at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... reaction mixture was supplemented with proteasome inhibitor MG132 (20 μM; Boston Biochem; #I-130). Reaction mixtures were incubated at 28°C ...
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bioRxiv - Neuroscience 2021Quote: ... The treated cells were incubated with the 5 µM proteasome activity probe Me4BodipyFl-Ahx3Leu3VS (Boston Biochem) for 2 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... purified proteasome (1 nM final concentration) was activated with PA28 (12nM final concentration; E380 Boston Biochem) followed by addition of Boc-LRR-AMC (50 µM final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... the bait GST or GST fusion protein was incubated with purified human 26S proteasome (E365; Boston Biochem, Cambridge, MA, USA) at 4 °C for 2 h in binding buffer (1× PBS ...
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bioRxiv - Microbiology 2020Quote: ... 100 µM of human wild-type (Boston Biochem) or K63R ubiquitin (2B Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... an equal amount of protein lysate (5 μg in 20 μl lysis buffer) was premixed with either 250 ng of proteasome inhibitor (epoxomicin, Boston Biochem, prepared in 50% dimethyl sulfoxide) or an equivalent volume of vehicle (50% dimethyl sulfoxide lacking the inhibitor) ...
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bioRxiv - Biochemistry 2021Quote: ... USP8 (wild-type or the mutants) was incubated with 25 ng/μl of Lys63-linked ubiquitin tetramers (Boston Biochem) in TBS with 10-mM dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence of hydrolyzed K63-linked diUb (TAMRA/QXL position 3 labeled, Boston Biochem #UF-330) was measured on a BioTek synergy LX plate reader equipped with a red filter cube assembly (ex ...
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bioRxiv - Genomics 2019Quote: ... For SUMOylation assays an antibody (A-603, Boston Biochem) directed against the SUMO1 substrate peptide (E-25K ...
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bioRxiv - Cancer Biology 2022Quote: ... The antibodies used were as follows: UBE2C (Boston Biochem, Cat# A650) and beta-actin (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Ubiquitination assays were performed at 37 °C for 90 minutes using loaded E1 (~ 80 ng, 3 μl, Boston Biochem kit, K-995), E2 (1 μM ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Commercial antibodies used for Western blotting analysis were as follow: anti-Ube2C (A-650, Boston Biochem), anti-ubiquitin (P4D1 ...
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bioRxiv - Cancer Biology 2022Quote: ... UBE2C protein levels were assessed by immunohistochemical (IHC) staining with UBE2C antibody (Boston Biochem, Cat# A650) or Ki-67 (D2H10 ...
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bioRxiv - Biochemistry 2023Quote: ... 25 ×106 cells per condition) and subjected to immunoprecipitation using 5 µg of ISG15 antibody (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... phosphor and protease inhibitor cocktails (25 x 106cells per condition) and subjected to immunoprecipitation using 5 μg ISG15 antibodies (Boston Biochem #A-380) plus 25 μL of protein G Sepharose slurry (Invitrogen ...