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Citations for Boston Biochem :
1 - 50 of 95 citations for Mouse E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... E3 ligase reaction buffer (Boston Biochem), and ATP in 25 μl reaction mixture ...
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bioRxiv - Cell Biology 2023Quote: ... Reactions were started using E3 ligase MDM2/HDM2 400 ng (Boston Biochem, E3-204), incubated for 3 hours at 30°C in a water bath ...
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bioRxiv - Molecular Biology 2020Quote: ... buffered with 1X E3 Ligase Reaction Buffer (Boston Biochem). Reactions were initiated by addition of Mg-ATP (Boston Biochem ...
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bioRxiv - Biochemistry 2022Quote: ... in E3 ligase reaction buffer (Boston Biochem B-71). The reaction was incubated at 37 °C for 2h and analysed by SDS-PAGE ...
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bioRxiv - Cell Biology 2019Quote: ... in a final volume of 30 μl of E3 ligase buffer (Boston Biochem, B-71) at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... The SCFFBW7 E3 ligase complex was incubated with recombinant 0.1 mM E1 (UBE1; Boston Biochem), 0.25 mM E2 (cdc34 ...
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bioRxiv - Immunology 2021Quote: ... E3 (0.5 μM, 6 μl, His-cIAP1, Boston Biochem, E3-280) and 15 μl of purified FLAG-IL-1β in Ubiquitin assay buffer containing 2 mM ATP (total volume of 30 μl) ...
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bioRxiv - Biochemistry 2023Quote: ... CF (Boston Biochem, E3-526) was employed for the initial Gel-Based ABPP screening ...
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bioRxiv - Neuroscience 2024Quote: ... non-phosphorylated PRKN (Boston Biochem, E3-160) and p-S65-Ub monomer (Boston Biochem ...
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bioRxiv - Biochemistry 2021Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 buffer (10% sucrose ...
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bioRxiv - Cell Biology 2020Quote: ... ubiquitin (10μM, Boston Biochem), E1 enzyme (150 nM ...
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bioRxiv - Molecular Biology 2019Quote: ... methylated ubiquitin (Boston Biochem) and ATP (Life technologies ...
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bioRxiv - Plant Biology 2022Quote: ... HIS6-ubiquitin (Boston Biochem), and GST-PUB30 or GST-PUB31 in the ubiquitination reaction buffer (0.1 M Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human ubiquitin, His6-ubiquitin, and ubiquitin KR mutants (K6R, K11R, K27R, K29R, K33R, K48R, K63R, K0) were purchased from Boston Biochem.
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bioRxiv - Cell Biology 2023Quote: ... 1.25 μM E2-UbcH5b (Boston Biochem, E3-622), and 2.5 μM p53 (Boston Biochem ...
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bioRxiv - Neuroscience 2024Quote: Recombinant p-S65-PRKN (Boston Biochem, E3-166), non-phosphorylated PRKN (Boston Biochem ...
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bioRxiv - Microbiology 2020Quote: ... 100 µM ubiquitin (Boston Biochem) and 5 mM ATP as indicated in ubiquitination assay buffer in 40 µL total reaction volume ...
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bioRxiv - Cancer Biology 2022Quote: ... Ubiquitin (200 μM, Boston Biochem) and recombinant RNF114 (1μg ...
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bioRxiv - Molecular Biology 2020Quote: ... ubiquitin at 50 μM (Boston Biochem), and Strep-tagged substrate (DDIT4 peak 1 ...
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bioRxiv - Biochemistry 2020Quote: ... ubiquitin aldehyde (U-201, Boston Biochem), creatine phosphokinase from rabbit muscle (C3755 ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μM ubiquitin aldehyde (Boston Biochem), 10 mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... Myc-ubiquitin (U-115, Boston Biochem), ubiquitin aldehyde (U-201 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 50 μM human ubiquitin (Boston Biochem), 4 mM ATP and 1 mM DTT in 20 mM MOPs ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 mM human ubiquitin (Boston Biochem) and 2 mM ATP (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 20 µM human ubiquitin (Boston Biochem) and 5 mM ATP as indicated in 40 µL ubiquitination assay buffer (50 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... 10 μg unlabeled ubiquitin (Boston Biochem), and 2 μg MBP-COP1 (previously incubated with 20 μM ZnCl2 ...
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bioRxiv - Biochemistry 2021Quote: Ubiquitin-AMC (Ub-AMC; Boston Biochem) hydrolysis assay was used to verify the wild-type and mutant USP14 deubiquitylating activities in the human proteasome ...
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bioRxiv - Cancer Biology 2022Quote: ... and Ubiquitin (200 mM, Boston Biochem) were incubated with the recombinant RNF114 (1 μg ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged ubiquitin mutants (Boston Biochem) were added at final concentrations of 1 µg/µL ...
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bioRxiv - Biochemistry 2023Quote: DDB1/CRBN (40 μg, Boston Biochem. Inc., E3-500-025) was diluted in PBS (100 uL ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... Ubiquitin vinyl sulfone tagged with HA (U-212) and HA-ubiquitin (U-110) purchased from Boston Biochem. For immunoprecipitation anti-HA-Agarose beads (A2095 ...
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bioRxiv - Cell Biology 2023Quote: The in vitro ubiquitination assay was performed using an ubiquitin kit (Boston Biochem, #K-230). His-tagged S5a protein (provided in the kit as positive control ...
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bioRxiv - Cancer Biology 2019Quote: ... or Flag-ubiquitin (30 μM, Boston Biochem) and were immediately laid on ProtoArray slides ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg of Myc-ubiquitin (Boston Biochem), 1 μM ubiquitin aldehyde (Boston Biochem) ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μg/ml ubiquitin (Boston Biochem) in the presence of purified BgaA-T and its variants ...
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bioRxiv - Biochemistry 2020Quote: ... or 5 µM tetra-ubiquitin (Boston Biochem) at RT ...
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bioRxiv - Plant Biology 2021Quote: ... 1.25 µg Flag tagged ubiquitin (Boston Biochem), 2 µg purified GST-PUB25 and PUB26 or HIS-PUB26 were added to 30 µL of ubiquitination reaction buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... 26 µM Ubiquitin (Boston Biochem U-530), 0.3 µM UBE1 (Boston Biochem E-304-050) ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-tagged human recombinant ubiquitin (Boston Biochem) was added to egg extracts at a final concentration of 0.1 μg/μL unless otherwise stated ...
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bioRxiv - Cell Biology 2023Quote: ... 0.6 μM ubiquitin (Boston Biochem, U-120), 225 nM E1-UBE-1 (Boston Biochem ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 nM GST-tagged Ubiquitin-activating enzyme E1 and 250 nM Ubiquitin-conjugating enzyme E2 UbcH5 [both from Boston Biochem, USA]) ...
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bioRxiv - Cell Biology 2019Quote: ... or human His6-ubiquitin (Boston Biochem, U-530), mouse E1 (Ube1 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 11.6 μmol ubiquitin (Boston Biochem #U-100H) in reaction buffer (0.2 M Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μM HA-ubiquitin (Boston Biochem U-110), 0.16 μM myc-Cyclin D1-CDK4 complex and 10 mM MgATP solution (Boston Biochem B-20 ...
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bioRxiv - Immunology 2019Quote: ... coli was added to 2 µg of K48-ubiquitin chains of mixed chain length (Ub2-Ub7) or to purified tetra-ubiquitin (Ub4) (#UC-230, UC-210, Boston Biochem). A20 OTU domains added to mixed-length K48-ubiquitin chains were incubated in 100 µl of deubiquitin buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2019Quote: ... 1 µM His-Ubiquitin (Boston Biochem, Cat#U-530), 50 nM mouse E1 enzyme ...
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bioRxiv - Biochemistry 2019Quote: ... 2 mg/ml Methylated ubiquitin (Boston Biochem #U-501) and 1 mM ATP at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Purified tetra-K63 ubiquitin was purchased from Boston Biochem. The following reagents were added to 20μl reactions where indicated ...
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bioRxiv - Cell Biology 2023Quote: ... UbcH5c and His-ubiquitin were purchased from Boston Biochem. E1 (50 nM) ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mg His-ubiquitin (BB-U-530, Boston Biochem), 2 mg purified MBP-KEG fusion protein (as E3 enzyme) ...