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1 - 13 of 13 citations for Cytomegalovirus gB Recombinant since 2019

• The X-linked intellectual disability gene product and E3 ubiquitin ligase KLHL15 degrades doublecortin proteins to constrain neuronal dendritogenesis

  Jianing Song, et al., bioRxiv - Biochemistry 2020

  Quote: ... recombinant human UBE1 (E-305, Boston Biochem), recombinant human UbcH5a/UBE2D1 (E2-616 ...
• Profiling ubiquitin signaling with UBIMAX reveals DNA damage- and SCF^βTRCP-dependent ubiquitylation of the actin-organizing protein Dbn1

  Camilla S. Colding-Christensen, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 6xHis-tagged human recombinant ubiquitin (Boston Biochem) was added to egg extracts at a final concentration of 0.1 μg/μL unless otherwise stated ...
• Multi-tiered actions of Legionella effectors to modulate host Rab10 dynamics

  Tomoko Kubori, et al., bioRxiv - Microbiology 2023

  Quote: ... 80 nM recombinant human E1 (Boston Biochem), 400 nM recombinant human E2 enzymes (Boston Biochem ...
• The X-linked intellectual disability gene product and E3 ubiquitin ligase KLHL15 degrades doublecortin proteins to constrain neuronal dendritogenesis

  Jianing Song, et al., bioRxiv - Biochemistry 2020

  Quote: ... recombinant human UbcH5a/UBE2D1 (E2-616, Boston Biochem), Myc-ubiquitin (U-115 ...
• Development and characterization of phospho-ubiquitin antibodies to monitor PINK1-PRKN signaling in cells and tissue

  Jens O. Watzlawik, et al., bioRxiv - Neuroscience 2024

  Quote: Recombinant p-S65-PRKN (Boston Biochem, E3-166), non-phosphorylated PRKN (Boston Biochem ...
• Multi-tiered actions of Legionella effectors to modulate host Rab10 dynamics

  Tomoko Kubori, et al., bioRxiv - Microbiology 2023

  Quote: ... 400 nM recombinant human E2 enzymes (Boston Biochem) and 400 nM purified E3 ligases in 50 mM Tris-Cl (pH 7.5) ...
• Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substrates

  Valentina Rossio, et al., bioRxiv - Biochemistry 2020

  Quote: ... Ubiquitin (U-100H) and recombinant DUBs were provided by Boston Biochem (E-519 ...
• Chemoproteomics-Enabled Ligand Screening Yields Covalent RNF114-Based Degraders that Mimic Natural Product Function

  Mai Luo, et al., bioRxiv - Biochemistry 2020

  Quote: ... Pure recombinant human RNF114 was purchased from Boston Biochem (K-220). RNF114 (0.25 μg ...
• The p97 cofactor Ubxn7 facilitates replisome disassembly during S-phase

  Zeynep Tarcan, et al., bioRxiv - Biochemistry 2021

  Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 buffer (10% sucrose ...
• Multi-tiered actions of Legionella effectors to modulate host Rab10 dynamics

  Tomoko Kubori, et al., bioRxiv - Microbiology 2023

  Quote: ... reaction mixtures (12.5 μl) containing 5 μg of recombinant human Ub (Boston Biochem), 80 nM recombinant human E1 (Boston Biochem) ...
• Kinetics characterization of ASXL1/2-mediated allosteric regulation of BAP1 deubiquitinase

  Hongzhuang Peng, et al., bioRxiv - Cancer Biology 2020

  Quote: ... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
• A novel innate pathogen sensing strategy involving ubiquitination of bacterial surface proteins

  Shruti Apte, et al., bioRxiv - Microbiology 2021

  Quote: ... The SCFFBW7 E3 ligase complex was incubated with recombinant 0.1 mM E1 (UBE1; Boston Biochem), 0.25 mM E2 (cdc34 ...
• Sde Proteins Coordinate Ubiquitin Utilization and Phosphoribosylation to Promote Establishment and Maintenance of the Legionella Replication Vacuole

  Kristin M Kotewicz, et al., bioRxiv - Microbiology 2023

  Quote: ... each 25 μl pelleted resin was resuspended with 4μg of recombinant human homotypic-linked polyubiquitin chains Ub3-7 (K63 or K48, 4μg/600μl reaction volume, Boston Biochem, R&D) and 500μM ε-NAD in 1X ART buffer for 2 hrs with gentle end-over-end rotation at 37°C ...