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Citations for Boston Biochem :
1 - 45 of 45 citations for 7 S 17 S dihydroxy 8 E 10 Z 13 Z 15 E 19 Z Docosapentaenoic Acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... through detection of the 7-amino-4-methylcoumarin (AMC) labeled fluorogenic peptide substrates Z-LLE-AMC (Boston Biochem #S-230) and LLVY-AMC (Chemicon #APT280) ...
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bioRxiv - Biochemistry 2021Quote: ... Boc-L-R-R-AMC (trypsin-like activity) and Z-L-L-E-AMC (caspase-like activity) (Boston Biochem). The proteasome samples were incubated with 50 μM AMC-peptide in the respective purification buffers (standard or the exogenous nucleotide depleted buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and STAMBPL1 (Boston Biochem #E-551). Reactions were measured with similar conditions as were used for measuring STAMBPJAMM inhibition ...
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bioRxiv - Biochemistry 2021Quote: ... inhibition was evaluated with full-length STAMBP (Boston Biochem #E-549) (activated with STAM1 (Boston Biochem #E-550)) and STAMBPL1 (Boston Biochem #E-551) ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant human UBE1 (E-305, Boston Biochem), recombinant human UbcH5a/UBE2D1 (E2-616 ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.85 µM UBE1 (Boston Biochem E-304-050), 5 mM adenosine triphosphate ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.3 µM UBE1 (Boston Biochem E-304-050), 1.5 µM UBE2T (Boston Biochem E2-695) ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.1 μg of UBE1 (Boston Biochem. Inc, E-305), 0.1 μg UBE2D1 (Boston Bichem ...
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bioRxiv - Cell Biology 2020Quote: ... 0.2 nM (#E-582 residues 57-517, Boston Biochem) was added and the plates pre-incubated for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 μg of UBE1 (Boston Biochem. Inc., E-305), 0.1 μg UBE2D1 (Boston Bichem ...
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bioRxiv - Cell Biology 2023Quote: ... 225 nM E1-UBE-1 (Boston Biochem, E-305), 1.25 μM E2-UbcH5b (Boston Biochem ...
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bioRxiv - Biochemistry 2020Quote: Suc-LLVY-AMC (Boston Biochem, Cat. # S-280)
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bioRxiv - Biochemistry 2021Quote: Suc-LLVY-AMC (Boston Biochem, Cat. # S-280)
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bioRxiv - Biochemistry 2021Quote: ... inhibition was evaluated with full-length STAMBP (Boston Biochem #E-549) (activated with STAM1 (Boston Biochem #E-550) ...
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bioRxiv - Biochemistry 2023Quote: ... UBE1 (0.4 μL, 5 μM, Boston Biochem. Inc., E-305-025), UBE2D1 (5.2 μL ...
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bioRxiv - Biochemistry 2023Quote: ... UBE1 (0.2 μL, 5 μM, Boston Biochem. Inc., E-305-025), UBE2D1 (2.6 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... in the presence of 5 nM of 20S proteasome (Boston Biochem, E-360), at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
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bioRxiv - Cell Biology 2019Quote: Immunopurified FLAG-BIRC6 on beads was incubated with the reaction mixture containing 125 nM GST-UBA6 (Boston Biochem, E-307), 6 μM 6xHis-LC3B (ProSpec Bio ...
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bioRxiv - Cell Biology 2024Quote: The in vitro ubiquitination reaction was performed in a 30 μl mixture containing 200 ng E1 enzyme (BB-E-304-050, Boston Biochem), 200 ng E2 enzyme (BB-E2-616-100 ...
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bioRxiv - Biochemistry 2022Quote: The ubiquitination assays were performed in a 25 μl reaction volume with the following components: 100 nM UBE1 (Boston Biochem E-304), 1.5 μM UBCH5C (Boston Biochem E2-627) ...
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bioRxiv - Cell Biology 2024Quote: ... The DUBs used in this study are USP2 (Enzo, BML-UW9850-0100, 1 µM and Boston Biochem, E-504-0650, 1 µM), vOTU (1 µM) ...
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bioRxiv - Biophysics 2024Quote: ... We added 10 μL of the enzyme:peptide mixture to 10 μL of 250 nM Ub-AMC (7-amino-4-methylcoumarin) (Boston Biochem). DUB activity was measured by monitoring the increase in AMC fluorescence in a plate reader ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15−7-amino-4-methylcourmarin (ISG15-AMC ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human ISG15−7-amino-4-methylcourmarin (ISG15-AMC) was purchased from Boston Biochem. Lys48 linked tetra-Ub were obtained from Boston Biochem ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were initiated by the addition of 250 nM ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, Boston Biochem) substrate to PfUBP-1 wt or catalytic inactive PfUBP-1 C3179A mutant ...
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bioRxiv - Biochemistry 2021Quote: ... We added 29 µM of the enzyme:peptide mixture to 1 mL of 30 µM Ub-AMC (7-amino-4- methylcoumarin) (Boston Biochem), for a final concentration of 200 nM DUBm ...
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bioRxiv - Microbiology 2023Quote: ... each 25 μl pelleted resin was resuspended with 4μg of recombinant human homotypic-linked polyubiquitin chains Ub3-7 (K63 or K48, 4μg/600μl reaction volume, Boston Biochem, R&D) and 500μM ε-NAD in 1X ART buffer for 2 hrs with gentle end-over-end rotation at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... 15 µl of 2x concentrated ubiquitin-rhodamine 110 (Ub-Rho110) (200 nM, final assay concentration = 100 nM; Boston Biochem), prepared in USP30 activity assay buffer ...
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bioRxiv - Plant Biology 2021Quote: ... 10 μg unlabeled ubiquitin (Boston Biochem), and 2 μg MBP-COP1 (previously incubated with 20 μM ZnCl2 ...
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bioRxiv - Molecular Biology 2020Quote: In vitro ubiquitylation reactions were carried out in a total volume of 15 μl with UBE1 at 0.2 μM (Boston Biochem), UbcH5b at 0.5 μM (Boston Biochem) ...
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bioRxiv - Biochemistry 2023Quote: DDB1 (3.3 uL, 8 μM) was mixed with CUL4A/NEDD8/RBX1 (0.2 uL, 9 μM, Boston Biochem. Inc., E3-441-025), UBE1 (0.2 μL ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg of Myc-ubiquitin (Boston Biochem), 1 μM ubiquitin aldehyde (Boston Biochem) ...
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bioRxiv - Cell Biology 2021Quote: ... energy regeneration solution (B-10, Boston Biochem) in assay buffer were carefully pipetted directly on the arrays ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5 and 0.5 mM ethylenediaminetetraacetic acid) containing a chymotryptic substrate (80 μM Suc-Leu-Leu-Val-Tyr -AMC; Boston Biochem). Reactions were incubated at 25°C for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μM HA-Ub (Boston Biochem, U-110), and 10 μM MgATP (Boston Biochem ...
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bioRxiv - Cell Biology 2019Quote: ... and 10 μM MgATP (Boston Biochem, B-20), in a final volume of 30 μl of E3 ligase buffer (Boston Biochem ...
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bioRxiv - Cell Biology 2019Quote: ... 200 nM BI2536 and 10 µM MG132 (Boston Biochem). Cycloheximide (CHX ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of 10 mg/ml Ub solution (Boston Biochem; #U-100H), and 1μl radiolabeled in vitro translated protein of interest ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 10 mg/ml Ubiquitin (Ub) solution (Boston Biochem; #U-100H) and 1 µl radiolabeled IVT product of interest ...
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bioRxiv - Biochemistry 2021Quote: ... were charged with 10 µg ubiquitin or N-terminally fluorescein-labelled Ub (FluoUb, Boston Biochem) using 0.02 µg ubiquitin-activating enzyme 1 (His6-E1 ...
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bioRxiv - Neuroscience 2021Quote: ... Sf 21 (baculovirus)-derived human USP30 protein) (10 nM; final assay concentration = 5 nM; Boston Biochem) was prepared in USP30 activity assay buffer (50 mM Tris base ...
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bioRxiv - Biochemistry 2022Quote: ... 0.16 μM myc-Cyclin D1-CDK4 complex and 10 mM MgATP solution (Boston Biochem B-20) in E3 ligase reaction buffer (Boston Biochem B-71) ...
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bioRxiv - Molecular Biology 2020Quote: ... translations were performed in the presence of 10 μM HA- or His-tagged Ubiquitin (Boston Biochem, USA). Denaturing immunoprecipitation of the ubiquitinated species was performed as previously described (Yanagitani et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 mM Na3VO4) at rt for 5-10 min before addition of master mix (42 pmol E1 (UBE1, Boston Biochem or Ubiquitin-Proteasome Biotechnologies) ...