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1 - 50 of 81 citations for Measles Virus Nucleoprotein HEK293 since 2019

• High number of HPAI H5 Virus Infections and Antibodies in Wild Carnivores in the Netherlands, 2020-2022

  Irina V. Chestakova, et al., bioRxiv - Microbiology 2023

  Quote: All samples were screened using the nucleoproteins of an H7N9 virus A/Anhui/1-BALF_RG6/2013 (Sino Biological) and H1N1 virus A/California/07/2009 (Sino Biological ...
• Polymeric Pathogen-like Particles-Based Combination Adjuvants Elicit Potent Mucosal T Cell Immunity to Influenza A Virus

  Brock Kingstad-Bakke, et al., bioRxiv - Immunology 2020

  Quote: PR8 nucleoprotein (NP) was purchased from Sino Biological Inc (Beijing ...
• Viral infection engenders bona fide and bystander lung memory B cell subsets through permissive selection

  Claude Gregoire, et al., bioRxiv - Immunology 2021

  Quote: ... nucleoprotein or SARS-CoV-2 spike protein (Sino biological). Labelling of recombinant proteins was carried out using APC and PE conjugation lightning-link kits (Abcam) ...
• The folate antagonist methotrexate diminishes replication of the coronavirus SARS-CoV-2 and enhances the antiviral efficacy of remdesivir in cell culture models

  Kim M. Stegmann, et al., bioRxiv - Molecular Biology 2020

  Quote: ... and Nucleoprotein (N; Sino Biological #40143-R019, 1:5000) overnight ...
• Trivalent SARS-CoV-2 S1 Subunit Protein Vaccination Induces Broad Humoral Responses in BALB/c Mice

  Muhammad S. Khan, et al., bioRxiv - Immunology 2022

  Quote: ... or rabbit anti-SARS-CoV nucleoprotein (1:3,000) (Sino Biological) was added and incubated overnight at 4°C as primary antibody ...
• Macrophages only sense infectious SARS-CoV-2 when they express sufficient ACE2 to permit viral entry, where rapid cytokine responses then limit viral replication

  Larisa I Labzin, et al., bioRxiv - Immunology 2023

  Quote: ... The following antibodies were used: SARS-CoV-2 Nucleoprotein (Sino Biological), Tubulin (9F3 ...
• The SARS-CoV-2 multibasic cleavage site facilitates early serine protease-mediated entry into organoid-derived human airway cells

  Anna Z. Mykytyn, et al., bioRxiv - Microbiology 2020

  Quote: ... or rabbit-anti-SARS-CoV nucleoprotein (40143-T62, 1:400, Sino biological). Tight junctions were stained using mouse-anti-ZO1 (ZO1-1A12 ...
• Structural basis of Blastomyces Endoglucanase-2 adjuvancy in anti-fungal and -viral immunity

  Lucas Dos Santos Dias, et al., bioRxiv - Immunology 2020

  Quote: ... with 10 μg Influenza A H1N1 nucleoprotein (Sino Biological Inc., 11675-V08B) alone ...
• A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

  Joep Beumer, et al., bioRxiv - Molecular Biology 2021

  Quote: ... primary antibodies used were mouse anti-nucleoprotein (1:200; 40143-MM05, Sino Biological), mouse anti-dsRNA (1:200 ...
• Co-infection of influenza A virus enhances SARS-CoV-2 infectivity

  Lei Bai, et al., bioRxiv - Microbiology 2020

  Quote: ... mouse monoclonal antibody against SARS-CoV Nucleoprotein (Sino Biological, 40143-MM05, 1:1000), anti-actin (Abclonal ...
• Proteolytic cleavage and inactivation of the TRMT1 tRNA modification enzyme by SARS-CoV-2 main protease

  Kejia Zhang, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Rabbit polyclonal anti-SARS-CoV-2 nucleoprotein N protein (40068-RP01; Sino Biological) and anti-actin (L00003 ...
• SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface

  Federico Bertoglio, et al., bioRxiv - Biochemistry 2020

  Quote: ... HEK293 (Sino Biological 40591-V08H); S1 ...
• SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface

  Federico Bertoglio, et al., bioRxiv - Biochemistry 2020

  Quote: ... HEK293 (Sino Biological 40592-V08H1); S1-RBD(V483A) ...
• SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface

  Federico Bertoglio, et al., bioRxiv - Biochemistry 2020

  Quote: ... HEK293 (Sino Biological 40592-V08H5); S1-RBD(R408I) ...
• SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface

  Federico Bertoglio, et al., bioRxiv - Biochemistry 2020

  Quote: ... HEK293 (Sino Biological 40602-V08H); Streptavidin Cy5 (Thermo Fisher 434316) ...
• SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface

  Federico Bertoglio, et al., bioRxiv - Biochemistry 2020

  Quote: ... HEK293 (Sino Biological 40592-V08H); S1-humFc ...
• SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface

  Federico Bertoglio, et al., bioRxiv - Biochemistry 2020

  Quote: ... HEK293 (Sino Biological 40591-V02H); S1S2 ...
• SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface

  Federico Bertoglio, et al., bioRxiv - Biochemistry 2020

  Quote: ... HEK293 (Sino Biological 40069-V08H); N ...
• SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface

  Federico Bertoglio, et al., bioRxiv - Biochemistry 2020

  Quote: ... HEK293 (Sino Biological 40592-V05H); S1-RBD32 ...
• SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface

  Federico Bertoglio, et al., bioRxiv - Biochemistry 2020

  Quote: ... HEK293 (Sino Biological 40591-V08H3); ...
• Time-Dependent Increase in Susceptibility and Severity of Secondary Bacterial Infection during SARS-CoV-2 Infection

  Amanda P. Smith, et al., bioRxiv - Microbiology 2022

  Quote: ... a primary monoclonal antibody against SARS-CoV-2 nucleoprotein (NP) (Sino Biological, Wayne, PA) was used at 1:1000 followed by a biotinylated anti-rabbit antibody (Vector Laboratories ...
• Viral infection engenders bona fide and bystander lung memory B cell subsets through permissive selection

  Claude Gregoire, et al., bioRxiv - Immunology 2021

  Quote: ... washed with PBS and coated overnight at 4°C with 1μg/ml of nucleoprotein (Sino Biological) diluted in PBS ...
• Pediatric nasal epithelial cells are less permissive to SARS-CoV-2 replication compared to adult cells

  Yanshan Zhu, et al., bioRxiv - Microbiology 2021

  Quote: ... rabbit anti-SARS-CoV-2 Nucleoprotein/NP antibody (1:1000 dilution, Cat#40143-R040, Sino Biological), goat polyclonal ACE2 (1:500 dilution ...
• Host Cell Proteases Drive Early or Late SARS-CoV-2 Penetration

  Jana Koch, et al., bioRxiv - Microbiology 2020

  Quote: The mouse mAb against the SARS-CoV nucleoprotein NP (40143-MM05) was purchased from Sino biologicals and used at dilutions of 1:500 for flow cytometry analysis and 1:1,000 for titration in TCID50 assays ...
• Sofosbuvir protects human brain organoids against SARS-CoV-2

  Pinar Mesci, et al., bioRxiv - Neuroscience 2020

  Quote: ... SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Antibody (rabbit Mab, Sino Biological #40143-R019, 1: 2000) was incubated diluted with blocking solution and incubated with cells for 20 minutes at room temperature ...
• Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation

  Mart M. Lamers, et al., bioRxiv - Microbiology 2021

  Quote: ... SARS-CoV-2 nucleoprotein was stained using rabbit-anti-SARS-CoV NP (1:1000, Sino Biological) and VSV nucleoprotein was stained using mouse-anti-VSV-N (1:1000 ...
• Determining the antiviral activity of two polyene macrolide antibiotics following treatment in Kidney Cells infected with SARS-CoV-2

  Kishor M. Wasan, Chris Galliano, bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... cells are fixed with paraformaldehyde and stained by anti-SARS-2 nucleoprotein monoclonal antibody (Sino Biological) followed by peroxidase-conjugated goat anti-mouse IgG (SeraCare) ...
• Dynamics of SARS-CoV-2 host cell interactions inferred from transcriptome analyses

  Lukas Adam, et al., bioRxiv - Systems Biology 2021

  Quote: We used antibodies to detect the SARS-CoV-2 nucleoprotein (mouse monoclonal; Sino Biologicals, Hong Kong, China) and GFP (mouse monoclonal ...
• Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation

  Mart M. Lamers, et al., bioRxiv - Microbiology 2021

  Quote: ... SARS-CoV-2 was stained with rabbit-anti-SARS-CoV nucleoprotein (40143-T62, 1:1000, Sino biological). Ciliated cells were stained with mouse-anti-AcTub (sc-23950 AF488 ...
• Prime-pull immunization of mice with a BcfA-adjuvanted vaccine elicits mucosal immunity and prevents SARS CoV-2 infection and pathology

  Mohamed M. Shamseldin, et al., bioRxiv - Immunology 2022

  Quote: ... After three days CPE was examined via staining for viral nucleoprotein (Sino Biological Cat. 40143-MM08-100). The left lung lobe was stored in 10% phosphate buffered formalin for 7 days prior to removal from the BSL3 for processing ...
• Pathogenesis, transmission and response to re-exposure of SARS-CoV-2 in domestic cats

  Angela M. Bosco-Lauth, et al., bioRxiv - Microbiology 2020

  Quote: ... Positive control for the nucleocapsid ELISA was SARS-CoV nucleoprotein rabbit monoclonal antibody (Sino Biological Inc, Beijing, China). Negative controls were reagent grade human sera (compared to Mab CR3022) ...
• N4-hydroxycytidine and inhibitors of dihydroorotate dehydrogenase synergistically suppress SARS-CoV-2 replication

  Kim M. Stegmann, et al., bioRxiv - Molecular Biology 2021

  Quote: ... primary antibodies were used to stain the SARS-CoV-2 Nucleoprotein (N; Sino Biological #40143-R019, 1:8000) and Spike protein (S ...
• A Novel Monoclonal Antibody Targeting a Large Surface of the Receptor Binding Motif Shows Pan-neutralizing SARS-CoV-2 Activity Including BQ.1.1 Variant

  Leire de Campos-Mata, et al., bioRxiv - Immunology 2023

  Quote: ... SARS-CoV-2 nucleoprotein was detected by immunohistochemistry (IHC) using the rabbit monoclonal antibody (40143-R019, Sino Biological) at a 1:15000 dilution ...
• Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin

  Yisong Qian, et al., bioRxiv - Immunology 2021

  Quote: ... MERS-CoV Nucleoprotein protein (40068-V08B) and SARS Coronavirus Nucleocapsid Protein (40143-V08B) were from Sino biological (Beijing, China). Mammalian expression plasmid for SARS-CoV-2 nucleocapsid protein (152536 ...
• Repurposing screen highlights broad-spectrum coronavirus antivirals and their host targets

  Sibylle Haid, et al., bioRxiv - Microbiology 2021

  Quote: ... Immunofluorescence staining was conducted using the SARS-CoV-2 nucleoprotein mouse monoclonal antibody (1:500; Sino Biological #40143-MM05), Alexa-Fluor Plus 488 anti-mouse antibody (1:1,000 ...
• Is myeloid-derived growth factor a ligand of the sphingosine-1-phosphate receptor 2?

  Yong-Shan Zheng, et al., bioRxiv - Biochemistry 2024

  Quote: ... coli (our present work) or HEK293 cells (from Sino Biological) was added (50 μl/well) ...
• Viral infection engenders bona fide and bystander lung memory B cell subsets through permissive selection

  Claude Gregoire, et al., bioRxiv - Immunology 2021

  Quote: ... ELISA (enzyme-linked immunosorbent assay) plates were coated overnight at 4°C with 1μg/ml of nucleoprotein or hemagglutinin (Sino Biological) diluted in PBS ...
• SARS-CoV-2 induces human plasmacytoid pre-dendritic cell diversification via UNC93B and IRAK4

  Fanny Onodi, et al., bioRxiv - Immunology 2021

  Quote: ... Cells were incubated for 1 hour at 4°C with 1μg/ml of anti-nucleoprotein mAb (40143-MM05; Sino Biological) diluted in permeabilization flow cytometry buffer (PBS supplemented with 5% FBS ...
• Resistance of endothelial cells to SARS-CoV-2 infection in vitro

  Blerina Ahmetaj-Shala, et al., bioRxiv - Immunology 2020

  Quote: ... Primary antibodies used were against spike protein (S) (Mouse monoclonal, Gene tex (1A9)) or nucleoprotein (N) (Rabbit monoclonal, Sino Biological). Following 3x PBS washes ...
• The SARS-CoV-2 multibasic cleavage site facilitates early serine protease-mediated entry into organoid-derived human airway cells

  Anna Z. Mykytyn, et al., bioRxiv - Microbiology 2020

  Quote: SARS-CoV-2 and SARS-CoV were stained with mouse-anti-SARS-CoV nucleoprotein (40143-MM05, 1:400, Sino Biological) or rabbit-anti-SARS-CoV nucleoprotein (40143-T62 ...
• A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

  Joep Beumer, et al., bioRxiv - Molecular Biology 2021

  Quote: ... permeabilized with 70% ethanol and stained with polyclonal rabbit anti-SARS-CoV nucleoprotein antibody (1:1000; 40588-T62, Sino Biological) followed by secondary Alexa488 conjugated goat-anti-rabbit antibody (Invitrogen) ...
• Human ACE2 peptide mimics block SARS-CoV-2 Pulmonary Cells Infection

  Philippe Karoyan, et al., bioRxiv - Pharmacology and Toxicology 2020

  Quote: Cell supernatants were collected at 48 h post-infection for Elisa assay using SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP ELISA Kit from Sino biological, according the manufacturer’s instruction ...
• SARS-CoV-2 neutralizing human antibodies protect against lower respiratory tract disease in a hamster model

  Bart L. Haagmans, et al., bioRxiv - Microbiology 2020

  Quote: ... and viral antigen was detected using a rabbit polyclonal antibody against SARS-CoV-nucleoprotein (40143-T62, Sino Biological, Chesterbrook, PA, USA) and horseradish peroxidase labeled goat-anti-rabbit IgG (P0448 ...
• CCR2 Regulates Vaccine-Induced Mucosal T-Cell Memory to Influenza A Virus

  Woojong Lee, et al., bioRxiv - Immunology 2021

  Quote: ... All vaccinations were administered intranasally to anesthetized mice in 50 ul saline with 10 ug NP (Influenza A H1N1 Nucleoprotein / NP Protein, Sino Biological) or 20ug DQ-OVA protein (Thermo Fisher Scientific ...
• Nafamostat Mesylate in lipid carrier for nasal SARS-CoV2 titer reduction in a hamster model

  Lisette Cornelissen, et al., bioRxiv - Microbiology 2020

  Quote: ... Sections were then incubated with a rabbit polyclonal antibody directed against recombinant SARS-CoV nucleoprotein (40143-T62, Sino Biological, Beijing, China). A goat anti-rabbit HRP polymer (Envision + ...
• Genome-scale CRISPR‒Cas9 screen identifies novel host factors as potential therapeutic targets for SARS-CoV-2 infection

  Madoka Sakai, et al., bioRxiv - Microbiology 2023

  Quote: ... Antigen-positive cells were detected by indirect immunofluorescence assay (IFA) using an anti-HCoV-229E nucleoprotein antibody (Sino Biological, Beijing, China).
• Differences in neuroinflammation in the olfactory bulb between D614G, Delta and Omicron BA.1 SARS-CoV-2 variants in the hamster model

  Lisa Bauer, et al., bioRxiv - Microbiology 2022

  Quote: ... Slides were then incubated with a rabbit polyclonal antibody against SARS-CoV/SARS-CoV-2-nucleoprotein (40143-T62, Sino Biological, Pennsylvania, USA) (1:1000) ...
• High number of HPAI H5 Virus Infections and Antibodies in Wild Carnivores in the Netherlands, 2020-2022

  Irina V. Chestakova, et al., bioRxiv - Microbiology 2023

  Quote: ... and H1N1 virus A/California/07/2009 (Sino Biological) using an arbitrary fluorescence cut-off of 6,000 ...
• SARS-CoV-2 B.1.351 (beta) variant shows enhanced infectivity in K18-hACE2 transgenic mice and expanded tropism to wildtype mice compared to B.1 variant

  Ferran Tarrés-Freixas, et al., bioRxiv - Microbiology 2021

  Quote: An immunohistochemistry (IHC) technique to detect SARS-CoV-2 nucleoprotein (NP) antigen using the rabbit monoclonal antibody (40143-R019, Sino Biological, Beijing, China) at a 1:15,000 dilution ...
• Anti-SARS-CoV-2 hyperimmune immunoglobulin provides potent and robust neutralization capacity and antibody-dependent cellular cytotoxicity and phagocytosis induction through N and S proteins

  José María Díez, et al., bioRxiv - Immunology 2021

  Quote: ... Potency was assessed using a cell-based immunosorbent assay to quantify infection by detecting the SARS-CoV-2 nucleoprotein using a specific antibody raised against this protein (Sino Biological, Wayne, PA). The secondary antibody (Life Technologies ...