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Citations for Sino Biological :
601 - 650 of 705 citations for Recombinant Mouse IL21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: The fusion proteins used in this study were expressed using transient gene expression in CHO cells as described previously (33,34) and purified from the cell culture medium to homogeneity by Protein A (Sino Biological) chromatography.
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bioRxiv - Microbiology 2020Quote: ... Plate was coated with SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag; Sino Biological, Cat# 40591-V08H) at 5 μg/ml at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... The 96-well plates coated with 0.1μg Spike protein of SARS-CoV-2 from Sino Biological (Product code: 40591-V08H) at 4°C overnight were blocked by 2% BSA/PBST at room temperature for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Monoclonal antibody (Ab) against SARS-CoV-2 spike protein (IgG1 clone#43, Cat # 40591-MM43) was purchased from Sino Biological, Inc (Wanye ...
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bioRxiv - Immunology 2021Quote: ... using a rabbit polyclonal antibody specific to SARS-CoV-2 RBD protein (cat. n° 40592-T62, Sino Biological, Beijing, China) (2/5000 ...
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bioRxiv - Biochemistry 2021Quote: Vero E6 cells were incubated with RBD protein (0.45 μg/mL, final concentration) followed by incubation with human anti-RBD antibody (primary antibody) (40150-D003, Sino Biological) and goat anti-human IgG AF488-conjugated antibody (secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates were coated with Spike 1 protein of SARS-CoV-2 (0.1μg/100 μL, Sino Biological, 40591-V08H), the tested sera were diluted at 1:100 and added to each well ...
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bioRxiv - Immunology 2022Quote: ... were coated with SARS-CoV-2 spike protein (SARS-CoV-2 S1+S2_ECD, 200 ng/well) (Sino Biological, Beijing, China) in PBS and incubated at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (1×106) were stimulated with SARS-CoV-2 spike protein (S1+S2_ECD, 5 μg/ml, Sino Biological, Beijing, China) or BSA (5 μg/ml ...
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bioRxiv - Immunology 2022Quote: Recombinant His-tagged Spike S1 wild-type produced in baculovirus-insect cells and with < 1.0 EU per μg protein as determined by the LAL method (#40591-V08B1, Sino Biological) or flagellin (Invivogen ...
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bioRxiv - Neuroscience 2022Quote: ... DRG tissue lysates were immunoprecipitated over 2 hours at 4°C with protein A/G coupled with antibodies (rabbit anti-FcγRI 1:1000, Cat: 80016-R015, Sino Biological Inc. ...
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bioRxiv - Synthetic Biology 2022Quote: ... were used to immobilize the peptide hormone and either Protein A (Fc tagged GLP-1 receptor, Sino Biological, 13944-H02H) or Ni-NTA (6x His tagged GH ...
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bioRxiv - Synthetic Biology 2022Quote: ... After bilayer formation beads were washed two more times with PBS and 5pmol of His-tagged proteins added (Sino Biological: ACE2-His 10108-H08H ...
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bioRxiv - Neuroscience 2024Quote: ... a rabbit polyclonal antibody against the SARS-CoV-2 nucleocapsid protein (dilution: 1:4,000, catalog number: 40143-T62, Sino Biological) was applied to the slides for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... which was composed of 20 µg of SARS-CoV-2 Spike S1+S2 trimer protein (10 µg D614G + 10 µg B.1.1.529) (40589-V08H8, 40589-V08H26, Sino Biological. Inc.), mixed with 20 µg of D-type CpG oligodeoxynucleotide (vac-1826-1 ...
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bioRxiv - Immunology 2022Quote: ... were coated with 15 μg/ml of anti-IgM and/or anti-IgG with or without 0.5 μg/ml of mouse histidine-tagged ICAM-1 (Sino Biological) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 and SARS-CoV were stained with mouse-anti-SARS-CoV nucleoprotein (40143-MM05, 1:400, Sino Biological) or rabbit-anti-SARS-CoV nucleoprotein (40143-T62 ...
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bioRxiv - Immunology 2023Quote: Mouse primary OPCs were treated with neutralizing antibody against TNFR2(anti-TNFR2; Cat#50128-RN204,RRID:AB_2860178,Sino Biological,6ug/mL) for 1 hour prior to exposure to pro-inflammatory cytokines ...
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bioRxiv - Immunology 2022Quote: ... at 4 °C for 30 min and then stained with biotin-conjugated SARS-CoV-2 RBD protein (Sino Biological, 40592-V05H) at 4 °C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... were prepared for H&E staining and immunofluorescence staining for SARS-CoV-2 detection using the Coronavirus nucleocapsid protein (NP) antibody (cat. 40143-MM05, Sino Biological). Images were collected under a Pannoramic MIDI system (3DHISTECH ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 wild-type (WT) and mutated (Beta: K417N-E484K-N501Y, Delta: L452R-T478K, Lambda: L452Q-F490S) RBD proteins (Sino Biological) were separately coated into 96-well plates at 4 °C overnight ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The L19-mTNF fusion protein was expressed by transient gene expression in CHO-S cells and purified from the cell culture supernatant to homogeneity by protein A (Sino Biological) chromatography ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with either 2 ug/ml SARS-CoV-2 Spike Protein ECD (Sino Biological, 40589-V08B1) or 1 ug/ml SARS-CoV-2 Spike-RBD (Sino Biological ...
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bioRxiv - Cancer Biology 2020Quote: ... the fusion protein was produced in CHO-S mammalian cells and purified from the cell culture medium by affinity chromatography using a Protein A (Sino Biological) affinity column ...
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bioRxiv - Microbiology 2021Quote: ... of 20 patients with COVID-19 obtained in February and October 2020 in Chongqing were previously reported.2 Eight RBD-specific mAbs with neutralizing capability against SARS-CoV-2 were obtained from the blood samples of COVID-19 convalescent patients.4 DNA sequences encoding reference strain (wild-type, WT) and mutant Spike proteins of SARS-CoV-2 were codon-optimized and synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Microbiology 2021Quote: The codon-optimized gene encoding reference strain (GenBank: QHD43416) SARS-CoV-2 Spike protein with C-terminal 19-amino acid deletion was synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Microbiology 2020Quote: The codon-optimized gene encoding SARS-CoV-2 S protein (GenBank: QHD43416) with C-terminal 19-amino acid deletion was synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Microbiology 2020Quote: ... The rabbit polyclonal antibody against SARS-CoV-2 SP (Cat# 40150-R007) and ACE2 protein (Cat# 40592-T62) were obtained from Sino Biological and anti-HIVp24 monoclonal antibody was described previously [35,36] ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Microbiology 2021Quote: ... The cells were formalin-fixed and ethanol permeabilized followed by incubation with a murine monoclonal antibody which targets the viral nucleocapsid protein (Sino Biological), followed by a secondary anti-mouse IgG peroxidase conjugate (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... RBD protein expression was analyzed using a rabbit polyclonal antibody SARS-CoV-2 Spike RBD Antibody (HRP) (Sino Biological, #40592-T62), which cross-reacts with SARS-CoV RBD protein ...
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bioRxiv - Microbiology 2021Quote: ... The binding of hACE2 to surface expressed S protein on infected HeLa cells was detected by incubating with 100 ng/106 cells of biotinylated human ACE2 protein (Cat# 10108-H08H-B, Sino Biological) followed by Alexa Fluor 647-conjugated anti-hACE2 antibody (Cat# FAB9332R ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant SARS-CoV-2 proteins S1-614D and S1-614G with polyhistidine-tag (Cat: 40591-V08H, 40591-V08H3) were purchased from Sino Biological. All proteins were quantitated with Qubit Protein Assay (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... specific S protein levels were identified using specific primary antibodies (NB100-56578, Novus Biologicals and 40150-T62-CoV2, Sino Biological Inc.), and HRP conjugated secondary antibody (Protein Simple) ...
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bioRxiv - Microbiology 2021Quote: ELISA plates (Evergreen Scientific) were coated with 100 μl per well of 1 μg/ml of SARS-CoV-2 S-ecto protein (Sino Biological), SARS-CoV-2 RBD-untagged protein (Sino Biological) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were treated with methanol 0.6% H2O2 and stained for 1 h with a 1:3000 dilution of 40143-R019 rabbit mAb to SARS-CoV-2 nucleocapsid protein (Sino Biological). A 1:3000 dilution of sheep anti-rabbit HRP conjugate (Sigma ...
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bioRxiv - Immunology 2020Quote: The following proteins were coated on 96 well ELISA plates at the following concentrations: SARS-CoV RBD (Sino Biological, 40150-V08B1) at 1 µg/ml ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 cells were fixed for 30 min in 4 % paraformaldehyde (PFA) and stained with a polyclonal antibody targeting the SARS-CoV-2 Nucleocapsid (N) protein (Sino Biological, catalogue number ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Western Blot detection of SARS-CoV-2 Spike protein was performed using a polyclonal Spike antibody (Sino Biological Cat. # 40589-V08B1).
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bioRxiv - Immunology 2021Quote: ... All vaccinations were administered intranasally to anesthetized mice in 50 ul saline with 10 ug NP (Influenza A H1N1 Nucleoprotein / NP Protein, Sino Biological) or 20ug DQ-OVA protein (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and huCD4 (Met 1-Trp 390; catalog no. 10400-H08H) proteins with C-terminus 6x-His-tags were purchased from Sino Biological.
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bioRxiv - Microbiology 2021Quote: The codon-optimized gene encoding SARS-CoV-2 spike (S) protein (GenBank: QHD43416) with 19 amino acids deletion at the C-terminal was synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Immunology 2020Quote: ... Similarly human ACE2 binding to surface expressed spike on MVA/S infected DF-1 cells was done using biotinylated human ACE2 protein (#10108-H08H-B, Sino Biological) followed by streptavidin-PE (BD Pharmingen ...
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bioRxiv - Microbiology 2021Quote: 250 μg the extracellular domain of SARS-CoV-2 spike protein fused with His tag (S1+S2 ECD, S, cat.# 40589-V08B1, Sino Biological) was emulsified with 250 μl Freund’s complete adjuvant (F5881-10ML ...
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bioRxiv - Immunology 2020Quote: ... Nbs were incubated with the bead-mix (containing beads coupled with SARS-CoV-2 homotrimeric spike, RBD and S1 proteins) and biotinylated ACE2 (Sino Biological) which competes for the binding of SARS-CoV-2 spike-derived antigens ...
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bioRxiv - Immunology 2021Quote: ... of the following SARS-CoV-2 derived antigens: S1+S2 subunits of the Spike (S) protein and receptor-binding domain (RBD, Sino Biological) were subsequently added and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with 3% BSA-PBS for 30 min to block nonspecific antibody binding and then incubated with SARS-CoV-2 nucleocapsid protein antibody (Sino Biological), followed by incubating with Alexa Fluor 488 (Thermo) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... two New Zealand white rabbits were immunized subcutaneously with 0.5 mg recombinantly expressed human Chi3l1 protein (Sino Biological, Cat: 11227-H08H). After the initial immunization ...
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bioRxiv - Microbiology 2021Quote: ... Calu3 cells were fixed for 30 min in 4 % paraformaldehyde (PFA) and stained with a polyclonal antibody targeting the SARS-CoV-2 Nucleocapsid (N) protein (Sino Biological, catalogue number ...