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Citations for New England Biolabs :
1 - 50 of 2154 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Positive control slides were treated with 3 U/mL DNase-1 (New England Biolabs, Cat. M0303) for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... with anti-Knbu/Kibu as the positive control using anti-butyryllysine antibody (PTM Biolabs, Cat# PTM-301) and histone H3 as the loading control using histone H3 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2020Quote: ... 1600 v/10 ms /3 pulses for 200,000 cells in Buffer R (Neon Transfection kit) premixed with 50 pmol Cas9 protein (CAT#M0646T, New England Biolabs), 50 pmol single guide RNA (sgRNA ...
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bioRxiv - Bioengineering 2019Quote: ... All BACs except DHFR BAC derived BACs were linearized before transfection: 2207K13-UG BAC with SgrAI (New England Biolabs, Cat. # R0603S), HBB-UG BAC with NotI (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... or a control motif (5’-GGGACCCTGGGAGGG-3’) were prepared by viral replication using a helper phage M13K07 (New England BioLabs, Cat#N0315S). E.coli XL1-Blue cells were transformed with pBluescript SK(- ...
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bioRxiv - Bioengineering 2024Quote: Linear dsDNA templates were made via PCR amplification was done using the GD2-CAR or no CAR Control plasmid as a template using Q5 Hot Start Polymerase (Cat # M0494S, NEB, Ipswich, MA) in 50 uL reaction volumes ...
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bioRxiv - Molecular Biology 2020Quote: ... These constructs were synthesized as gBlocks (Integrated DNA Technologies) and PCR-amplified before transfection using Q5 polymerase (New England Biolabs, Cat No. M0491S). To generate the repair template ...
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bioRxiv - Neuroscience 2023Quote: ... rTg4510 mouse brain sections were incubated in the absence (control) or presence (treated) of 10,000 U/mL λ phosphatase (New England Biolabs, cat. no. P0753) for 24 hours at RT ...
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bioRxiv - Molecular Biology 2023Quote: The linear ssDNA scaffold for our designs as the positive control and negative control was obtained by cutting circular M13mp18 DNA (New England Biolabs) at BamHI-HF and EcoRI-HF (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... Before transfection p70intsce was linearized with Not1 (NEB).
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bioRxiv - Cell Biology 2020Quote: ... Constructs were linearized for transfection with NotI (NEB) and transfected into the 29-13 cell line ...
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bioRxiv - Immunology 2023Quote: mHSP70 or fetuin (New England Biolabs, positive control) were subjected to cleavage by PNGase F (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: ... or amylose resin (negative control; New England Biolabs) at 4°C for 3 h ...
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bioRxiv - Immunology 2020Quote: ... A positive control on the other hand was established by transforming DH5α bacteria with NEBuilder® Positive Control (New England Biolabs, USA). After transformation ...
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bioRxiv - Cell Biology 2021Quote: ... m6A-modified RNA spike-in controls (New England Biolabs) and a nontargeted region on the crRNA-targeted transcript ...
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bioRxiv - Cell Biology 2019Quote: ... Untreated controls were supplemented with GlycoBuffer3 (New England Biolabs). Reactions were stopped by treatment with SDS-PAGE sample buffer containing benzonase nuclease (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... As a control Escherichia coli ribosomes (P0763S, NEB, Australia) were used in 4 μL aliquots (approx ...
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bioRxiv - Molecular Biology 2023Quote: ... BamHI (Site 2) or HindIII (Control; New England Biolabs) overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Before transfection pKD plasmids were digested overnight with EcoRV (NEB). The enzyme was then subjected to heat inactivation for 20 min at 65 °C and then mixed with the pUF1-Cas9 plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... or positive controls doxorubicin (50 μM; New England Biolabs 5927) and antimycin A (10 μM ...
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bioRxiv - Immunology 2019Quote: ... pSV40-CLuc control plasmid was obtained from NEB (Ipswich, MA). These constructs were co-transfected to 293 cells by FugeneHD (Promega) ...
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bioRxiv - Developmental Biology 2020Quote: ... Controls were handled with same protocol without λ-phosphatase (NEB). Phospho-mass spectrometry was performed as previously described (Nagai et al. ...
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bioRxiv - Microbiology 2020Quote: ... Endo H controls: Cells were treated with Endo H (NEB, 1:10 in glycobuffer 3 ...
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bioRxiv - Cell Biology 2021Quote: ... The m6A-modified and unmodified control RNAs (New England Biolabs) were divided into fragmented RNA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... SNAP-tag comes from pSNAPf-ADRβ2 control plasmid (NEB, #N9184). SNAP-CB2-TwinStrep-1D4 ...
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bioRxiv - Cancer Biology 2023Quote: ... A negative control treated overnight with RNaseH1 (New England Biolabs) was included ...
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bioRxiv - Genomics 2020Quote: Transfection of gRNA and Cas9 ribonucleoprotein (EnGen SpyCas9, New England Biolabs) into mESCs was performed using Neon Transfection System ...
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bioRxiv - Cell Biology 2020Quote: ... Renilla luciferase was then ligated into pGL3-Control digested HindIII (NEB) to make pGL3-Renilla-FF ...
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bioRxiv - Developmental Biology 2021Quote: 4mC and 5mC spike-in controls were generated from pUC19 (NEB) PCR product synthesized with N4-methyl-dCTP (4mdCTP ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µg BSA dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 µl coating buffer (100 mM Tris-HCL pH 8 ...
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bioRxiv - Immunology 2021Quote: ... or an appropriate isotype-matched control (polyclonal goat isotype IgG, NEB) was added at a final concentration of 10 μg/ml and incubated overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested 30 hours after transfection using Passive Lysis Buffer (NEB) and luciferase activity determined with Bright-Glo luciferin reagent (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... Unmethylated control plasmid was produced in dam− bacteria (New England BioLabs #C2925H) and methylation status was confirmed by DpnI and DpnII digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into the control plasmid using restriction enzyme XhoI (NEB, #R146L). pCAGGS-Cre plasmid was generated as described (Kranz et al. ...
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bioRxiv - Cell Biology 2021Quote: ... A positive control was prepared by substituting casein kinase II(NEB, P6010S) for galectin 3 in the reaction mixture.
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bioRxiv - Biochemistry 2023Quote: ... of the SARS-CoV-2 Positive Control (N gene) plasmid (NEB N2117). Sanger sequencing confirmed successful mutagenesis ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μg BSA per well dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 μl coating buffer (100 mM Tris-HCL pH 8 ...
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bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
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bioRxiv - Cell Biology 2023Quote: ... and pSNAPf-H2B control plasmid (Addgene #101124, New England Biolabs and Ana Egana) using KpnI and NotI ...
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bioRxiv - Immunology 2021Quote: ... Cat.# R0101S)/BamHI (NEB; Cat.# R0136S) fragment to generate GFP-YVAD-RFP (Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was extracted in native conditions and digested by restriction enzyme cocktail fragmentation (BsrGI-HF NEB cat. R3575, EcoRI-HF NEB cat. R3101, HindIII-HF NEB cat. R31045, SspI-HF Neb cat. R3132 and XbaI NEB cat. R01455). Immunoprecipitation was performed using 8 µg of fragmented DNA incubated for 14h at 16°C with 20 µg of S9.6 antibody (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... the FLuc Control Template from the HiScribe T7 high yield RNA synthesis kit (NEB) was in vitro transcribed and purified as described the linearized pTXB1 vector.
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bioRxiv - Immunology 2022Quote: ... As an isotype control an unspecific polyclonal rabbit antibody was used (NEB, Frankfurt, Germany). To block ORF8 during differentiation it was preincubated with the polyclonal rabbit anti-ORF8 antibody or isotype over night and added to the monocytes (t=0 ...
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bioRxiv - Biochemistry 2021Quote: ... Control samples containing only RNA were treated with RNA 5’ Pyrophosphohydrolase (RppH, NEB, M0356S) to convert 5’-PPP RNA into 5’-P RNA ...
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bioRxiv - Genomics 2020Quote: ... As a control plasmid pCMV6 plasmid (4.6kb) was linearized by digestion with NdeI (NEB, RO111). 180 ng control linearized plasmid DNA and 180 ng of 3C library was treated with either 0.5 Unit exonuclease V (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... Library concentration and quality control were determined using NEBNext Library Quant Kit (New England Biolabs) and Agilent High Sensitivity D1000 ScreenTape System (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... together with controls were subject to enzymatic digestion by PNGase F (P0708L, New England Biolabs), Endo H (P0702L ...
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bioRxiv - Cell Biology 2022Quote: ... and either incubated with buffer control or lambda protein phosphatase (λ) (New England Biolabs; P0753) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... A fully methylated control sample (CpG Methylated HeLa Genomic DNA; New England BioLabs, MA, USA) was included in a random position on each plate to facilitate plate tracking ...
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bioRxiv - Immunology 2020Quote: ... m6A-positive and m6A-negative control oligonucleotides (EpiMark N6-Methyladenosine Enrichment Kit, New England Biolabs) were spiked into total RNA prior to immunoprecipitation ...