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Citations for New England Biolabs :
1 - 35 of 35 citations for p p' DDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: 500mM p-Nitrophenyl Phosphate (pNPP, NEB, P0757S) substrate solution was diluted to 20 mM in phosphatase reaction buffer (PRB ...
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bioRxiv - Bioengineering 2024Quote: ... M13mp18 circular DNA (P- 107, Bayou Biolabs) was used as the scaffold for the staples (IDT) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was prepared from total RNA using the rflP specific primer (P-LCM621) and template-switching oligonucleotide (P-LCM619) using RT Template Switching Enzyme Mix (New England Biolabs). The 5’-end end of the rflP cDNA was enriched by PCR using the primers P-LCM620 and P-LCM622 ...
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bioRxiv - Neuroscience 2022Quote: ... p-GSK-3β (S9) (NEB D85E12, 1:50,000), GSK-3β (NEB 27C10 ...
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bioRxiv - Neuroscience 2023Quote: ... and p{ywing2+} were combined by HiFi assembly (NEB). The product was transformed into DH5α Chemically Competent Cells (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: TEV protease (TEV-P) was purchased from NEB (Cat#P8112S). PFFs were prepared as above and premixed 1:10 (for a final concentration of 15 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’P was created by T4 PNK treatment (NEB, M0201S), done according to the NEB T4 PNK protocol ...
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bioRxiv - Microbiology 2020Quote: ... we used a colorimetric assay using p-Nitrophenyl phosphate (pNPP) (NEB) as a substrate ...
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bioRxiv - Synthetic Biology 2022Quote: Rectangle: 10 nM of M13mp18 ssDNA scaffold (Bayou Biolabs, P-107) was thermo-ramped in 20x excess of ssDNA staples strands ...
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bioRxiv - Microbiology 2020Quote: ... the source plasmid (p-retronWT) was mutagenized through PCR using a kit (NEB; Q5-Site-Directed Mutagenesis Kit ...
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bioRxiv - Plant Biology 2022Quote: ... and 6 µM p-Nitrophenyl Phosphate (pNPP) (New England Biolabs Catalog Number P0757S) at 25°C at a pH of 5.0 ...
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bioRxiv - Microbiology 2019Quote: ... then 100 μL of 4 mg/mL p-nitrophenyl phosphate (New England Biolabs, MA, USA) was added and the mixture was vortexed again ...
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bioRxiv - Genomics 2021Quote: ... to 3’-P presenting in the RNA that was dephosphorylated using T4 PNK (NEB, #M0201S). Linker and RNA ligation was performed by ligating pre-adenylated linker to the 3’-OH of RNA using RNA ligase2 ...
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bioRxiv - Bioengineering 2023Quote: ... Rectangle DNs were folded with 10 nM of M13mp18 ssDNA scaffold (Bayou Biolabs, P-107) and annealed in 20x excess staples ...
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bioRxiv - Genetics 2021Quote: ... and the fragments were assembled into p(bhsp68-Cas9) using NEBuilder HiFi assembly (NEB, Ipswich, MA). The hsp68-nls-Cas9-nls-T2A-EGFP-nanos3’UTR cassette was excised using flanking AscI sites and ligated into AscI-linearized pBac-3XP3-v+ ((Siebert et al ...
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bioRxiv - Genomics 2022Quote: ... digested empty vector A or P by gibson assembly NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621) in an 80 μL reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were treated with JET (12.5 nM d-o-p) and/or ScaI-HF (NEB, 5 U) for 15 minutes at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... p-SFG retroviral backbone containing an IRES-ΔCD19 reporter gene was linearized by NotI-HF (NEB, ref: R3189S) and XhoI (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... and then constructed the library using NEBNext® Ultra™ II for DNA kit (NEB; P/N E7645L). We used the Agilent Bioanalyzer to assess the quality of the library and a qPCR approach with the KAPA Library Quantification Kit (Roche ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: The WT and terminator mutant versions of p-PBAD-gfpmut3-cerN-ig-plcH-nLuc were built using HiFi (NEB) assembly using either synthetic gene fragment cerN-wtTerm-nLuc or cerN-mutTerm-nLuc and HindIII cut pMQ80 ...
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bioRxiv - Molecular Biology 2021Quote: ... The 3’ adapter (RNA oligo: 5’P-GAUCGUCGGACUGUAGAACUCUGAAC-3’InvdT) was pre-adenylated prior to use (5’ DNA adenylation kit, NEB, according to the manufacturer’s instructions) ...
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bioRxiv - Genomics 2019Quote: ... 3’ P ends of RNA were dephosphorylated by resuspending bead-nuclei in 190 µL dephosphorylation solution (1× PNK buffer (NEB), 0.1% Triton X-100 ...
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bioRxiv - Genomics 2019Quote: ... fragmented nascent RNA was dissolved in H2O and incubated with 10 pmol of reverse 3’ RNA adaptor (5’p-rNrNrNrNrNrNrGrArUrCrGrUrCrGrGrArCrUrGrUrArGrArArCrUrCrUrGrArArC-/3’InvdT/) and T4 RNA ligase I (NEB) under manufacturer’s conditions for 2 h at 20°C ...
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bioRxiv - Microbiology 2021Quote: The resulting fragment was ligated into VSV NG-P (Jia et al., 2020) plasmid linearized with MluI and NotI using T4 ligase (NEB).
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bioRxiv - Microbiology 2020Quote: ... targeting the C-terminus of the TgPPM3C gene was cloned into the p-HXGPRT-Cas9-GFP plasmid backbone using KLD reactions (New England Biolabs), as previously described [49] ...
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Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylationbioRxiv - Biophysics 2019Quote: ... 1 unit of λ-phosphatase is defined as the amount of enzyme that hydrolyzes 1 nmol of p-nitrophenyl phosphate in 1 minute at 30°C (New England Biolabs). After 45 minutes of incubation ...
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bioRxiv - Cell Biology 2019Quote: ... the pGL4.10-P plasmid was mutated by PCR using mutant primers and Q5 site-directed mutagenesis kit (New England Biolabs, E0554) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... targeting the C- or N-terminus of various genes were cloned into the p-HXGPRT-Cas9-GFP plasmid backbone using KLD reactions (New England Biolabs), as previously described (71) ...
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bioRxiv - Microbiology 2020Quote: ... were created by replacing the entire env-coding sequence of F-MLV WT with amphotropic env-coding sequence from p-SV-A MLV env using NEBuilder HiFi DNA Assembly Kit (New England Biolabs). The primers used for swapping the env-coding regions were ...
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bioRxiv - Microbiology 2021Quote: ... Point mutations were introduced in the P and N sequences by site-directed mutagenesis using the Q5 site-directed mutagenesis Kit (New England Biolabs). Sequence analysis was carried out to check the integrity of all constructs ...
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bioRxiv - Genetics 2023Quote: ... The PCR product was digested with an enzyme that gains or loses a restriction site due to the specific P/LP variant in that fragment (Table 1, NEB). Digestion was performed according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Library preparation was slightly different and performed using the NEBNext® rRNA Depletion kit and protocol (NEB; P/N: E7850X) per manufacturer recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplified PCR products were ligated into the recombinant plasmid pZE0-P-MglE-T using the recommended standard USER cloning protocol (NEB, UK).
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bioRxiv - Molecular Biology 2020Quote: Synthetic RNA fragments bearing a 3′P were subjected to 5′ phosphorylation with T4 PNK 3′ minus (NEB, cat n° M0236S), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... 10 μg of Δorf library plasmid was digested with 100 units of AsiSI for vector A or PmeI for vector P (NEB, R0630 or R0560) at 37°C overnight ...