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Citations for New England Biolabs :
1 - 50 of 1157 citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... RNA was reverse transcribed with Moloney murine leukemia virus (M-MuLV) transcriptase (New England Biolabs). The purity and concentration of RNA were measured using NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... using 200-300 ng of purified RNA as template and Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase (M0253, NEB). cDNA was synthesised using the standard first strand synthesis protocol with random hexamers (S1230 ...
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bioRxiv - Physiology 2023Quote: ... was obtained by reverse transcription (RT) using 1 μg of RNA and Moloney murine leukaemia virus (M-MuLV) reverse transcriptase (M0253, NEB), using the first strand cDNA synthesis standard protocol with random primers (S1330 ...
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bioRxiv - Genetics 2020Quote: ... 0.75 M NaCl) and 2 μl protein kinase K (NEB, Ipswich, USA) during 2 h at 60 °C ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2021Quote: Env solubilized from cell lysates and VLPs was denatured by boiling in denaturing buffer (New England Biolabs) for 10 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... E protein glycosylation status was determined by treating RVP lysates with PNGase F (Cat#P0704S, New England Biolabs) for 3 hours at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... using the Vaccinia virus Capping enzyme (NEB) and biotinylated RNA species were subsequently enriched by affinity purification using streptavidin beads yielding 0.6 to 1.3% of the sRNA preparation ...
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bioRxiv - Microbiology 2019Quote: ... 50 µg of BSM or 5% v/v washed erythrocytes in PBS were treated with 1:100 NA VLPs or 1:100 Arthrobacter ureafaciens NA (NeuA, New England BioLabs) for 4 hours at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... we dilute the stock DNA to a final concentration of 6 mg/mL with 1/10th volume of 10× CutSmart Buffer (0.5 M Potassium Acetate, 0.2 M Tris-acetate, 0.1 M Magnesium Acetate; New England BioLabs), 0.1% Tween (to prevent non-specific interactions) ...
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bioRxiv - Molecular Biology 2022Quote: ... The commercially available vaccinia virus capping system (NEB #M2080S) was used as a positive control.
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of each sgRNA and 60 pmol Cas9-NLS protein (EnGen® Spy Cas9 NLS, NEB, M0646 M) with the Lonza 4D X-unit ...
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bioRxiv - Cancer Biology 2022Quote: ... AAK1(S-676D/E/A) and UVRAG (T-518D/E/A) using Q5-site directed mutagenesis Kit (NEB). Manufacturer’s instructions for mutagenic primer design were followed ...
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bioRxiv - Biochemistry 2019Quote: ... 37°C with vaccinia virus capping enzyme (New England Biolabs), as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and Moloney murine leukemia virus reverse transcriptase (New England Biolabs). Using a QuantStudio 3 real-time quantitative PCR machine (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... 0.09 μL DNA ligase (E. coli) (NEB, M0205) and 0.09 μL Ambion RNase H (E ...
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bioRxiv - Microbiology 2021Quote: ... T7 SHuffle (C3026, E. coli K strain, NEB) and Nico (λDE3 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5ul DNA ligase (E. coli, NEB cat. M0205S), 2ul DNA polymerase I (E.coli ...
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bioRxiv - Zoology 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Evry, France) and random hexamer primers were used to transcribe 1 μg RNA in a 20 μl reaction into cDNA.
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bioRxiv - Developmental Biology 2021Quote: ... All oligos were radioactively capped using Vaccinia virus capping system (NEB) and [α-32P]-GTP (Perkin-Elmer) ...
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bioRxiv - Cell Biology 2022Quote: ... and transformed into competent DH5a E. coli (5-alpha Competent E. coli) following the protocol provided by the company (New England Biolabs, Ipswich, MA). For colony selection ...
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bioRxiv - Genomics 2022Quote: ... The pellet was resuspended in 1 mL of NEB and centrifuged at 11,000rpm for 20 min on a sucrose gradient (0.65 mL of 1.6 M sucrose in NEB, 0.35 mL of 0.8 M sucrose in NEB). The Nuclei pellet was then resuspended in 1 mL of NEB ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei-containing pellets were resuspended in 1 mL of NEB + 0,25 M sucrose and centrifuged at 20000xg for 20 min on sucrose gradient (0.65 mL of 1.6 M sucrose in NEB, 0.35 mL of 0.8 M sucrose in NEB). The pellet was resuspended in 1 mL of NEB and formaldehyde to a final concentration of 1% ...
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bioRxiv - Neuroscience 2020Quote: ... 0.35 μL DNA polymerase I (E. coli) (NEB, M0209), 0.09 μL DNA ligase (E ...
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bioRxiv - Immunology 2021Quote: ... E coli C2566 cells (New England Biolabs, Ipswich, MA) were transformed with the CuMVTT coat protein (CP ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... MBP-CENP-E was cloned into pMal-C2X (NEB). Bacterially-expressed constructs of GST-CENP-E were cloned in pET-3aTr (Tan ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... Escherichia coli (E. coli strain BL21, New England BioLabs) and Listeria monocytogenes (L ...
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bioRxiv - Molecular Biology 2024Quote: Escherichia coli (E. coli) strains such as DH5α (NEB), BL21 DE3 (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... and transformation (chemically competent NEB 5- alpha E. coli) were performed according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and M-MuLV reverse transcriptase (NEB) with buffer ...
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bioRxiv - Genomics 2022Quote: ... 200 μ M GTP (NEB, N0450S)).The run-on reaction was stopped by adding Trizol LS (Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... and Phusion polymerase (M-0530, NEB) was used when DNA fragments were sequenced ...
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bioRxiv - Genomics 2022Quote: ... and Phusion polymerase (M-0530, NEB) was used when DNA fragments were sequenced or used for molecular cloning ...
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bioRxiv - Genomics 2023Quote: ... and Phusion polymerase (M-0530, NEB) was used when DNA fragments were sequenced or used for molecular cloning ...
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bioRxiv - Neuroscience 2023Quote: ... M-MLV RT buffer (NEB; B0253S) 1X ...
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bioRxiv - Molecular Biology 2023Quote: ... M-MuLV Reverse Transcriptase (NEB, M0253L), RNase inhibitor (NEB ...
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bioRxiv - Molecular Biology 2021Quote: All eight gene segments of the isolated H6N1 virus were amplified (NEB), purified (Omega ...
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bioRxiv - Genomics 2022Quote: Library E was transformed into 5-alpha E.coli (NEB, C2987H), recovered in SOC medium (NEB ...
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bioRxiv - Microbiology 2022Quote: ... coli dam+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/ dcm− (dam−/dcm− competent E ...
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bioRxiv - Microbiology 2021Quote: ... and M-MuLV reverse transcriptase (NEB M0253). For quantitative RT-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... M-1 was digested with AflII (NEB) overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 U M-MuLV RT (NEB) in RT Buffer (25 mM KCl ...
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bioRxiv - Molecular Biology 2020Quote: ... M-MuLV reverse transcriptase (New England Biolabs) and oligo-d(T ...
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bioRxiv - Systems Biology 2023Quote: ... and M-MuLV Reverse Transcriptase (NEB M0253). qPCR was performed in biological and technical triplicate using an Applied Biosystems QuantStudio 3 Real-Time PCR System with Fast SYBR Green Master Mix (Thermo Fisher 4385617) ...
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bioRxiv - Microbiology 2021Quote: Escherichia coli (NEB® 10-beta Electrocompetent E. coli or NEB® 5-alpha Competent E ...
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bioRxiv - Microbiology 2021Quote: ... Virus stocks were recovered following linearization of infectious clone plasmids using NotI-HF (NEB), in vitro transcription ...
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bioRxiv - Microbiology 2022Quote: ... The virus lysate was then denatured by boiling in denaturing buffer (New England BioLabs) for 10 min and treated with PNGase F or Endo Hf enzymes (New England BioLabs ...
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bioRxiv - Biophysics 2023Quote: ... The mRNAs were capped using the vaccinia virus capping enzyme kit (New England Biolabs) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... MeV-M BiFC constructs were made by exchanging NiV-M fused to VN173 or VC155 for MeV-M using the Gibson Assembly Cloning Kit (New England BioLabs). All mutant plasmids were generated using the Q5 site-directed mutagenesis kit using PAGE-purified mutagenesis primers designed using the online NEBase Changer primer design tool (New England BioLabs) ...