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Citations for New England Biolabs :
1 - 21 of 21 citations for Vitamins Trace Elements and Antioxidant Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 0.6 μL oxidation supplement (NEB), 0.6 μL oxidation enhancer (NEB) ...
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bioRxiv - Genomics 2023Quote: ... 0.3 μL oxidation supplement (NEB), 0.3 μL oxidation enhancer (NEB) ...
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bioRxiv - Bioengineering 2020Quote: ... with supplements (16 U RNase inhibitor (NEB), 5 mM FMN) ...
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bioRxiv - Microbiology 2021Quote: ... amplified mini-CRISPR elements were digested by KpnI and BamHI (NEB, USA) and were ligated into pAY5211 employing the Quick LigationTM Kit (NEB ...
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bioRxiv - Developmental Biology 2023Quote: Regulatory elements were amplified from mouse genomic DNA with Q5 polymerase (NEB, M0491) using primers listed in Table S8 and cloned into pGL4.24[luc2P/minP] (Promega ...
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bioRxiv - Biophysics 2021Quote: ... according to the protocol for cloning DNA containing repeat elements (C3040, New England BioLabs Inc.). In both plasmids ...
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bioRxiv - Microbiology 2019Quote: ... Mini-CRISPR elements and plasmid pAY5211 were digested using the restriction enzymes KpnI and BamHI (NEB, USA) and ligated using the Quick Ligation Kit (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... The bicistronic reporters used to detect regulatory elements within 3′ UTRs were constructed by Gibson Assembly (NEB) and standard methods ...
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bioRxiv - Molecular Biology 2020Quote: ... all sequences between transposable elements of a p2T plasmid were removed by restriction digestion using AleI and EcoRI (NEB). The TetO-eGFP-HygroR cassette was created by amplifying each subunit individually from already excising constructs ...
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bioRxiv - Genetics 2022Quote: ... L1 elements were then reconstructed by combination of non-mutated fragments from different clones using restriction enzymes (New England Biolabs) cutting within the L1 sequence.
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bioRxiv - Bioengineering 2022Quote: ... Emerald GFP was subcloned in-frame downstream of the gvpNJKFGWV ORF via a P2A linker and the entire pNJKFGWV-GFP ORF was subcloned into a pCMVSport backbone with WPRE-hGH-poly(A) elements using NEBuilder HiFi DNA Assembly (NEB). gvpA-IRES-EBFP2-WPRE-hGH polyA was constructed by Gibson assembly of a PCR-amplified IRES-EBFP2 fragment into the XbaI site of gvpA--WPRE-hGH polyA plasmid.
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bioRxiv - Microbiology 2022Quote: ... The library of circular DNA fragments was then used as a PCR template with forward and reverse primers specific to the transposable element and amplified using Phusion High-Fidelity DNA Polymerase (New England Biolabs). PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: IS element excision from the plasmid backbone was detected by PCR using OneTaq 2X Master Mix with Standard Buffer (NEB) and 0.2 uM primers ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNAs were subcloned downstream of a tetracycline response element (TRE) using the Gibson Assembly Cloning kit (New England Biolabs, # E5510S). Mutations were introduced by in vitro mutagenesis using Q5 Site-directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Synthetic Biology 2019Quote: ... The three backbone fragments were combined together with the synthesized CggR cis-regulatory element using the NEB Gibson assembly kit (New England Biolabs, MA, US) according to the manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR amplicons of furnished FPs were digested with BsmBI enzymes and following clean-up all three elements were ligated with T4 DNA ligase (NEB, Ipswich, MA).
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bioRxiv - Synthetic Biology 2023Quote: ... PCRs for cloning and of high GC Streptomyces elements were performed using Q5® High-Fidelity DNA Polymerase with GC Enhancer (New England BioLabs Inc., U.S.A). Colony PCRs for crRNA integrations into pCRISPR-Cas3 were performed using OneTaq® 2X Master Mix with Standard Buffer Enhancer (New England BioLabs Inc. ...
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bioRxiv - Genomics 2023Quote: ... The DNA with end tags was oxidated by adding 15 μL TET2 reaction mix (6 μL TET2 reaction buffer plus reconstituted TET2 reaction buffer supplement (NEB), 0.6 μL oxidation supplement (NEB) ...
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bioRxiv - Genomics 2023Quote: ... The DNA with end tags was oxidized in 15 μL TET2 reaction mix (3 μL TET2 reaction buffer plus reconstituted TET2 reaction buffer supplement (NEB), 0.3 μL oxidation supplement (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA was extracted using full B2M locus primers (Supplement Table 1) and amplified with Q5® Hot Start 2x Master Mix (New England Biolabs, Ipswich, Massachusetts, US). The target DNA was purified by PureLink® PCR cleanup (Invitrogen) ...