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Citations for New England Biolabs :
1 - 50 of 1706 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 0.3 μL oxidation supplement (NEB), 0.3 μL oxidation enhancer (NEB) ...
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bioRxiv - Genomics 2023Quote: ... 0.6 μL oxidation supplement (NEB), 0.6 μL oxidation enhancer (NEB) ...
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bioRxiv - Bioengineering 2020Quote: ... with supplements (16 U RNase inhibitor (NEB), 5 mM FMN) ...
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bioRxiv - Microbiology 2024Quote: ... The cultures were monitored using the Cell Growth Quantifier (Aquila Biolabs) with readings every 60 s and incubated at 37 °C and 210 rpm until the late stationary phase was reached ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated from the cell culture supernatants and converted to cDNA (NEB). The E gene of stock virus ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from 0.25×106 cells at day 12 of cell culture using the Monarch total RNA miniprep kit (New England Biolabs, T2010S) and quantified using the Qubit RNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... the culture filtrates from methanol-induced cultures were treated with PNGaseF (New England Biolabs), and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis ...
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bioRxiv - Biophysics 2021Quote: ... 5% CO2 -balanced complete cell culture medium with 5 μM SNAP-Surface Alexa Fluor 647 (NEB, S9136S), for 15 minutes at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... the bank vole lung homogenate and Mygla.REC.B cell culture supernatant were treated with a cocktail of micrococcal nuclease (New England BioLabs) and benzonase (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... gibco) supplemented with 10% fetal calf serum (eurobio scientific) and 1% ZellShield cell culture contamination preventive (Minerva-Biolabs). Cells were passaged at 80-90% confluency ...
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bioRxiv - Neuroscience 2020Quote: ... The holder allowed immersion of the cochlea and the middle ear in oxygenated (95 % O2, 5 % CO2) cell culture medium (Minimum Essential Medium with Earle’s balanced salts, SH30244.FS Nordic Biolabs). The bone of the bulla was removed gently with bone cutters which exposed the middle ear and the basal turn of the cochlea ...
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bioRxiv - Microbiology 2020Quote: ... TMR-Star was added to cells in culture samples inside an anaerobic chamber to a final concentration of 250 nM (New England Biolabs) and the mixture incubated for 30 minutes in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of Universal nuclease (Pierce; 125U/L of culture) and 10 μl of DNaseI (NEB; 20U/L of culture) were added and the mixture allowed to incubate for 10 min at RT ...
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bioRxiv - Genomics 2020Quote: ... Cultures were infected with 8ml M13K07 helper (NEB) for 60min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 50 mL cell culture were grown in LB to stationary phase and processed with the NEB Monarch genomic DNA purification kit (NEB #T3010). For genomic DNA extraction of E ...
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bioRxiv - Genetics 2021Quote: ... Plasmid was isolated from the remainder of the original 5-mL culture of the R599A culture using standard methods and digested with PvuI-HF (New England Biolabs #R3151) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... each culture was recovered in provided outgrowth media (NEB) by shaking at 37°C at 250 rpm for 1.5 hours ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 500ng of RNA (tissue samples) or 100ng RNA (cell culture experiments) using the LunaScript RT Supermix kit (New England Biolabs, Cat. No. E3010L) according to the manufacturer’s directions ...
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bioRxiv - Genomics 2023Quote: ... The DNA with end tags was oxidized in 15 μL TET2 reaction mix (3 μL TET2 reaction buffer plus reconstituted TET2 reaction buffer supplement (NEB), 0.3 μL oxidation supplement (NEB) ...
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bioRxiv - Genomics 2023Quote: ... The DNA with end tags was oxidated by adding 15 μL TET2 reaction mix (6 μL TET2 reaction buffer plus reconstituted TET2 reaction buffer supplement (NEB), 0.6 μL oxidation supplement (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... xanthus cultures using the Monarch Total RNA Miniprep Kit (NEB). 109 cells were harvested and resuspended in 200 µl lysis-buffer (100 mM Tris-HCl pH 7.6 ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA was extracted using full B2M locus primers (Supplement Table 1) and amplified with Q5® Hot Start 2x Master Mix (New England Biolabs, Ipswich, Massachusetts, US). The target DNA was purified by PureLink® PCR cleanup (Invitrogen) ...
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bioRxiv - Genomics 2020Quote: ... Plasmid DNA was purified from liquid cultures using DNA miniprep kit (NEB #T1010).
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bioRxiv - Microbiology 2022Quote: DNA was extracted from single-colony cultures (Monarch Genomic DNA Purification Kit, NEB). Short read libraries were prepared based on the NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... untreated cultures of cortical neurons (DIV8) were lysed in lambda phosphatase buffer (NEB), incubated for 20 min to ensure total lysis ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants (150 μL) were treated with DNase I (NEB, Ipswich, MA, USA) to obtain only enveloped and encapsidated viral genomes ...
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bioRxiv - Microbiology 2023Quote: ... coli overnight cultures using a Monarch plasmid miniprep kit (New England Biolabs, USA) and concentration was quantified by NanoDrop (Thermo Scientific ...
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bioRxiv - Genetics 2021Quote: MBP-Stwl antigen was purified from induced bacterial culture using Amylose Resin (NEB: E8021L). Briefly ...
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bioRxiv - Bioengineering 2019Quote: ... the culture media mix was TSB with 1 mg/mL BSA (New England Biolabs) and 50 μM calcein ...
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bioRxiv - Biochemistry 2023Quote: ... Samples of E.coli cultures were harvested and resuspended in E.coli lysis reagent (NEB, US). Lysates and purified proteins were separated by 4-12% Bis-Tris SDS-PAGE (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... DNA extracted from the 30mL culture was digested with HindIII-HF (#R3104S, New England Biolabs) and BamH1-HF (#R3136S ...
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bioRxiv - Microbiology 2023Quote: Turicibacter genes were amplified from template culture lysates with Phusion or Q5 DNA polymerase (NEB) and primers designed to amplify denoted Turicibacter genes ...
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA were extracted from bacterial cultures using Monarch Plasmid Miniprep Kit (New England Biolabs) and sequenced using the BigDye Terminator v.1.1 kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... the HeLa cells were kept in HeLa culture media treated overnight with 0.2 U/mL of □2-3,6,8,9 Neuraminidase (New England Biolabs) to cleave sialic acid from the cell surface glycans.
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bioRxiv - Biochemistry 2021Quote: ... This was added to 5 ml (per 2 L of culture) amylose resin (New England Biolabs) equilibrated in lysis buffer and left on a tube roller shaker at 4 °C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids from O/N cultures were extracted with the Monarch Plasmid Miniprep Kit (New England Biolabs) and sent for sequencing to LGC genomics.
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bioRxiv - Molecular Biology 2022Quote: ... was added to the culture prior to RNA extraction using Monarch total RNA miniprep kit (NEB). Isolated RNA was quantified using Quibit RNA BR assay kit (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... the plasmid was isolated from the dense culture using the Monarch® Plasmid Miniprep Kit (NEB) and the DNA was eluted with nuclease-free water ...
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bioRxiv - Systems Biology 2023Quote: ... we pelleted each liquid culture by centrifugation and used the Monarch Plasmid Miniprep Kit (NEB #T1010L) to isolate plasmid according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples collected from culture media were treated with PNGase F enzyme (New England Biolabs, P0709S) to remove the N-linked oligosaccharides from the TECTA protein according to the manufacturer protocol ...
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bioRxiv - Microbiology 2022Quote: ... Freshly prepared supercoiled plasmid DNA was then isolated from overnight cultures using a Monarch miniprep kit (NEB) and then immediately subjected to restriction digestion with T7 endonuclease (NEB ...
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bioRxiv - Genomics 2020Quote: ... Plasmid libraries were harvested from liquid culture using the Monarch® Plasmid DNA Miniprep Kit (NEB #T1010). Miniprep kit reagents were scaled to accommodate one spin column for every 5mL of culture.
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA was quantified using a Qubit 4 fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Cleared bacterial lysate from 1L of culture was incubated with 2mL of equilibrated amylose resin (NEB #E8021L) in a rotator overnight at 4C ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA was quantified using a Qubit 4 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted from the cocktail culture by Monarch Total RNA Miniprep Kit including DNase I (NEB, Ipswich, MA) which was confirmed by multiplex RT-PCR and gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... each 180 μl sample/Saccharibacteria co-culture was treated with Endo Hf Kit P0703L (New England Biolabs, Evry, France): 3 μl of each reagent was added to the sample and incubated for one hour at room temperature ...