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Citations for New England Biolabs :
1 - 50 of 9204 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... pREP81-FLAG vector and plasmids carrying wild type and mutated forms of rrp1+ and rrp2+ as well as domains of rrp1+ gene used in the yeast two hybrid system were constructed using the Gibson Assembly® Cloning Kit (NEB). All primers used to amplify gene sequences by PCR are listed in Table S3 ...
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bioRxiv - Microbiology 2021Quote: All bacterial two-hybrid plasmids were constructed using the Gibson assembly mix (NEB) or the Hifi DNA assembly mix (NEB) ...
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bioRxiv - Genetics 2023Quote: Plasmids for yeast two-hybrid experiments were constructed using NEBuilder DNA assembly (NEB), Ligation High kit (TOYOBO) ...
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bioRxiv - Developmental Biology 2020Quote: ... and dlk-1a were cloned into the two-hybrid vectors using HiFi Assembly (NEB).
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bioRxiv - Plant Biology 2021Quote: ... Library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs Inc.) from 1 µg RNA ...
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bioRxiv - Genetics 2021Quote: ... library construction was performed using the NEBNext Ultra-II kit (New England Biolabs) following manufacturer recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... before library construction using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB). The libraries were sequenced on an Illumina NoveSeq 6000-PE150 ...
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bioRxiv - Cancer Biology 2023Quote: ... Illumina Standard library construction (New England Biolabs) and sequencing (Illumina NovaSeq ...
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bioRxiv - Cancer Biology 2020Quote: ... Library construction was performed using the NEBNext UltraII DNA Library Prep Kit from NEB (E7645S). Indexed samples were run using the Illumina Next-seq 300-cycle kit ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA-Seq library construction was done using NEBNext UltraRNA Library Prep Kit for Illumina (NEB) at Novogene Corporation Inc ...
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bioRxiv - Pathology 2019Quote: ... Library construction was done using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) following manufacturer’s protocols ...
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bioRxiv - Genomics 2023Quote: ... (Sacramento, CA, USA) for library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs) from 1 µg total RNA and Illumina sequencing ...
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bioRxiv - Microbiology 2020Quote: ... followed by library construction via NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Each experimental treatment was performed in triplicate ...
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bioRxiv - Developmental Biology 2020Quote: ... Library construction was done using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Prior to stranded mRNA isolation library construction using NEBnext library kits (New England Biolabs, Evry, France), the quality of the RNA pool was assessed on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... and library construction was executed using NEBNext Ultra RNA Library Prep kit (New England Biolabs, USA), and sequenced on a NovaSeq6000 ...
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bioRxiv - Microbiology 2024Quote: ... The extracted nucleic acid was then subject to library construction using NEBNext Ultra RNA Library Prep Kit and NEB Next Ultra DNA Library Prep Kit (LTD.NEB, China) for RNA and DNA samples ...
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bioRxiv - Microbiology 2021Quote: ... Gibson assembly was used for plasmid construction (NEB). amyE gene encoding for α-amylase was amplified from genomic DNA of B ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... library construction (New England Biolabs, E7490 and E7760), and sequencing of paired 150 bp reads in an Illumina NovaSeq ...
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bioRxiv - Microbiology 2022Quote: ... We used two high fidelity PCR systems—Q5 (NEB cat. M0492), and Platinum SuperFi II (Invitrogen cat ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was then used for library construction with NEBNext Ultra II Directional RNA Library Prep Kit (NEB). Libraries were sequenced on Illumina Hiseq X10 (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... Library construction was done using the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s protocol.
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bioRxiv - Systems Biology 2023Quote: Library construction was carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S, NEB). 4ng of fragmented DNA was used for end-repair/A-tailing ...
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bioRxiv - Molecular Biology 2023Quote: ... and all constructions and intermediates were generated using a NEBuilder HiFi DNA assembly cloning kit (E5520S, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... DNA library construction was performed using the NEBNext Ultra II DNA Library Prep Kit from NEB (E7645L). The libraries were sequenced on Illumina NovaSeq 6000 system (50 bp paired-end ...
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bioRxiv - Genetics 2023Quote: ... and genomic DNA libraries were prepared using the NEBNext genomic DNA library construction kit (New England Biolabs). DNA libraries were sequenced on an Illumina Hi-Seq and deep sequencing reads were analyzed using standard methods on Galaxy ...
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bioRxiv - Pathology 2021Quote: ... The kit used for library construction was the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Total RNA extracted from the samples was subject to mRNA enrichment using poly-T oligo-attached magnetic beads ...
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bioRxiv - Genomics 2023Quote: ... The DNA/RNA hybrid strand was pre-adenylated with DNA 5’ Adenylation Kit (NEB) and purified with Oligo Clean & Concentrator (Zymo) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Enzymes used for plasmid construction were purchased from NEB. Primers were obtained from IDT and Eurofins genomics ...
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bioRxiv - Biochemistry 2023Quote: ... and IMut5SB constructs were created by a two-fragment assembly system (NEB), replacing WT INCENP1–58 sequence with the corresponding gBlock Gene Fragments (IDT) ...
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bioRxiv - Developmental Biology 2021Quote: ... and subjected to Tru-seq library construction using NEBNext Ultra II DNA Library Prep kit (NEB, Cat E7645S) as standard protocols ...
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bioRxiv - Developmental Biology 2020Quote: ... ChIP-Seq library construction was done using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The obtained DNA fragments were subjected to library construction using the NEBNext™ DNA Library Prep Kit (NEB), following the supplier’s specifications ...
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bioRxiv - Biochemistry 2021Quote: ... Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB), and the resulting library was sequenced using Illumina MiSeq v2 (2×150 bp ...
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bioRxiv - Genomics 2022Quote: ... Three library construction were performed with the NEBNext DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA). The sequencing was carried out by Illumina HiSeq 2000 platform (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid construction was carried out using enzymes obtained from NEB (NEBuilder HiFi DNA assembly master mix ...
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bioRxiv - Cancer Biology 2021Quote: ... before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Adaptors were removed from the 3’ end of reads using cutadapt (Martin ...
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bioRxiv - Molecular Biology 2020Quote: EM-seq library construction was performed using the NEBNext Enzymatic Methyl-seq Kit (New England BioLabs, Ipswich, MA, USA) according to manufacturer instructions with modifications ...
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bioRxiv - Immunology 2020Quote: ... Library construction for RNA-seq was performed using NEBNext RNA Ultra I kit (New England Biolabs, catalog number E7530L) with polyA mRNA isolation module (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: ... and then used for 350-bp paired-end library construction with the Next Ultra DNA library prep kit (NEB) for Illumina sequencing ...
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bioRxiv - Genetics 2020Quote: ... Bisulfite conversion and library construction were carried out as before using the EpiMark Bisulfite Conversion Kit (NEB cat# E3318) according to manufacturer’s specifications ...
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bioRxiv - Plant Biology 2022Quote: ... followed by RNA-seq libraries construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB E7420). RNA libraries were sequenced on an Illumina NovaSeq 6000 platform (PE150 bp) ...
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bioRxiv - Genomics 2023Quote: ... or 100 cGy) was processed for bisulfite sequencing library construction using NEBNext Ultra II DNA Library Prep Kit (NEB) along with methylated and indexed NEXTflex Bisulfite-seq Barcodes (Bio scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... About 1ng DNA was used for library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Sequencing (2× 150bp ...
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bioRxiv - Cell Biology 2022Quote: ... and CUT&RUN samples from two replicate (two independent CRISPRi clones) using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645, NEB). The manufacture’s protocol was employed with the following changes ...
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bioRxiv - Biophysics 2022Quote: ... This construction was used as a template to introduce the mutation W330A using the Q5-Site Direct Mutagenesis Kit (NEB) with the oligonucleotides W330A-fw 5′-GAGCGGTACCGCCCTGACCTATACCG- and W330A-rv 5′-GGGGTAACTTCCATGCCA- ...
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bioRxiv - Molecular Biology 2020Quote: ... and sequencing libraries were prepared using the NEB ULTRA kit following manufacturer’s instructions for genomic DNA library construction and using methylated adaptors (NEB E7535S). Adaptor-ligated DNA was isolated by 2% agarose gel electrophoresis (350–400 bp) ...
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bioRxiv - Molecular Biology 2019Quote: ... rRNA-depleted RNA was used as a starting material for library construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Two biological replicates per condition were sequenced on Illumina HiSeq 2500 instrument at Millard and Muriel Jacobs Genetics and Genomics Laboratory at Caltech yielding 15-20 million single-end 50-bp reads ...
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bioRxiv - Genomics 2021Quote: ... or by ribodepletion with NEBNext Human/Mouse/Rat rRNA Depletion kit followed by library construction using the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Illumina 2×151 paired end reads were generated either on the HiSeq 4000 or NovaSeq 6000 sequencing platforms (Illumina) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The extracted RNA was prepared for short-read sequencing library construction using the NEB NextUltra RNA-seq kit (New England Biolabs). The constructed libraries were sequenced with 150bp paired-end reads on an Illumina HiSeq 2500 and 6000 sequencing platforms ...