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Citations for New England Biolabs :
1 - 50 of 249 citations for Transmembrane Serine Protease 11A TMPRSS11A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and the prototype serine endopeptidase trypsin (New England BioLabs), placed within a fluorescence plate reader (Spectra max Gemini XPS ...
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bioRxiv - Cell Biology 2020Quote: ... where the Emp24 transmembrane domain (residues 173-193) was replaced by 26 leucines using Gibson assembly (New England Biolabs). To construct pSP-FLAG-Cp ...
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bioRxiv - Cell Biology 2024Quote: ... Serine-35 mutations were constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers are listed in the supplemental methods.
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: ... and aspartic acid 134 to serine using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Austria). The sequences were verified by both DNA and amino acid sequencing ...
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bioRxiv - Microbiology 2022Quote: ... EE62/63AA and the 2_87 double serine phospho-mimetic SE53/57EE or “SS-EE”) using Phusion-II polymerase (New England Biolabs) and standard protocols ...
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bioRxiv - Biochemistry 2023Quote: ... serine or aspartic acid were generated by engineering the corresponding constructs using Q5 site-directed mutagenesis (New England Biolabs, E0554). For this purpose ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... WT hNEMF was generated by mutating the serine at site 86 to an arginine using Q5 site-directed mutagenesis (NEB, E0554S). pBI-dsRED empty vector was cloned using annealing primers flanked by NheI and EcoRV ...
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bioRxiv - Cell Biology 2023Quote: ... The C-KSR-GS construct replacing the vector-based linker (LELKLRILQS) by a glycine-serine rich linker (GGSSGGGGA) was generated by site directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, USA) of the C-KSR-EGFP plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... Protease K (P8107S, NEB). Antibodies used were ...
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bioRxiv - Biophysics 2020Quote: ... Factor Xa protease (New England Biolabs) was added to the MT solution at a molar ratio of 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x protease inhibitor cocktail (NEB), 2 mM N-ethylmaleimide (NEM) ...
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bioRxiv - Biochemistry 2021Quote: ... or V8 protease (New England Biolabs) in 25 mM NH4HCO3 overnight at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 20 µg factor Xa protease (NEB) and 5 mM CaCl2 with inversion at 10 °C overnight ...
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bioRxiv - Biophysics 2021Quote: Time-resolved experiments with TEV protease were performed by adding 1 μL TEV protease (New England Biolabs) after the 3rd time point to an imaging well containing 100 μL protein sample ...
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bioRxiv - Biophysics 2023Quote: ... concentrated protein was supplemented with 100 U TEV-His7 protease in 1X TEV protease reaction buffer (NEB) and incubated at 30 °C with agitation for 3 hr to cleave the His6-tag ...
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bioRxiv - Neuroscience 2021Quote: ... nuclei were resuspended in 200uL digestion buffer with 4 uL protease (an equal volume of protease K (NEB) was substituted if the manufacturer-provided protease was exhausted ...
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bioRxiv - Biochemistry 2022Quote: ... 3µL of TEV protease (New England BioLabs) was added per 200µL of CrossMAb-ACE2 fusions at ∼2µM in 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... 2µL of TEV protease (New England BioLabs) was added per 200 µL of scFv-ACE2 fusions at ∼4µM in 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... 1X protease inhibitor cocktail (New England Biolabs)] ...
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bioRxiv - Biophysics 2021Quote: ... and was TEV-protease (50 units; NEB) cleaved overnight at 4°C to remove the APEX tag ...
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bioRxiv - Plant Biology 2021Quote: ... or TEV protease (P8112S, New England BioLabs) were used for the cleavage of constructs in the backbone of pMAL-c5x and for His-MBP-[TEV]-ADCK3 ...
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bioRxiv - Genomics 2019Quote: ... The embryos were treated with protease (1 µL of 25 µg/µL Qiagen Protease, 1 µL of 10x NEB Buffer 4 ...
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bioRxiv - Biochemistry 2020Quote: ... the resin was incubated with TEV Protease (NEB) for 3 h at room temperature ...
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bioRxiv - Genetics 2019Quote: ... and cleavage by TEV protease (New England Biolabs). The eluted chromatin was then used for a second purification step using Ni-NTA Agarose with nickel-charged affinity resin (Thermo Fisher) ...
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bioRxiv - Genetics 2020Quote: ... The indicated amount of furin protease (NEB P8077) was added per reaction tube in a final volume of 20 μL ...
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bioRxiv - Genomics 2020Quote: ... 0.5% NP40 and 1x protease inhibitors (P8107S, NEB) on ice for 15 min ...
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bioRxiv - Biochemistry 2019Quote: ... and 3 protease inhibitor cocktail tablets (New England Biolabs) were added to the cell suspension ...
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bioRxiv - Molecular Biology 2019Quote: ... 500 nL of protease mix was added (1x NEB CutSmart buffer ...
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bioRxiv - Cell Biology 2021Quote: ... samples were digested by protease K (New England Biolabs), eluted and cross-link-reversed ...
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bioRxiv - Genomics 2021Quote: ... 250 nL of protease mix was added (1× NEB CutSmart buffer ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of TEV protease (New England Biolabs, #P8112S) was added ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were cleaved with TEV protease (New England Biolabs) and further purified via reverse IMAC ...
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bioRxiv - Microbiology 2021Quote: F(ab’)2 fragments were generated using IdeZ protease (NEB), purified using CaptureSelect LC-lambda affinity matrix (human ...
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bioRxiv - Biophysics 2021Quote: ... The desalted sample was then incubated with TEV protease (NEB) (50 units ...
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bioRxiv - Microbiology 2020Quote: ... the resin was treated with factor Xa protease (P8010L, NEB) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... 1X protease inhibitor cocktail ± lambda protein phosphatase (New England Biolabs) as per the manufacturer’s instructions and incubated at 30°C for one hour.
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bioRxiv - Neuroscience 2020Quote: TEV protease (TEV-P) was purchased from NEB (Cat#P8112S). PFFs were prepared as above and premixed 1:10 (for a final concentration of 15 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... with the addition of 1 µL GluC protease (P8100S, NEB) at 1 mg/mL per mg of RH5ΔNL (1:1000 wt/wt) ...
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bioRxiv - Neuroscience 2022Quote: ... These solutions were added to 100 units of TEV protease (NEB) in aggregation buffer ...
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bioRxiv - Immunology 2020Quote: ... 0.09% Tween-20) containing 0.2 mg/ml protease K (NEB, P8107S) and incubating at 56°C for 1 hour ...
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CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal regionbioRxiv - Biochemistry 2020Quote: ... Samples were deproteinized with 0.5 mg/ml of Protease K (NEB) and incubated for 1 hrs at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... 1/20-1/50 (w/w) GluC protease (New England BioLabs) was added and incubated 24-48 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The ubiquitin (in vivo) and 3xFlag-TEV (using TEV protease, NEB) fusions were removed to reveal three N-terminal glycines required for N-terminal sortase-mediated labeling ...
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bioRxiv - Biochemistry 2021Quote: ... His6-mTRPM7-KD was subjected to TEV protease (New England Biolabs) digestion according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the GST-6xHis-tag was removed by TEV protease (#P8112S, NEB) and dArc1 and dArc2 proteins were further purified by size exclusion chromatography using Superdex S200 16/600 (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2024Quote: ... Protease Inhibitors) with recombinant PKA kinase (2500 U/mg, NEB-P600S) and 1 mM ATP overnight at 30°C and 250 rpm ...
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bioRxiv - Biochemistry 2024Quote: Purified MBP-fusion proteins were treated with TEV-protease (NEB, P8112S) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... TFIIIC was recovered after overnight incubation with TEV protease (100 units, NEB). TFIIIC was empirically determined to be saturating and TFIIIB and Pol III activities were determined to be limiting by multiple-round transcription assays.
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bioRxiv - Plant Biology 2022Quote: ... 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 x Protease Inhibitor Cocktail (NEB) as described before (Sun et al. ...
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bioRxiv - Bioengineering 2021Quote: ... the eluted MBP-RFP-sspB proteins were cut with TEV protease (NEB) using a digestion site between the MBP and the RPF sections ...