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Citations for New England Biolabs :
1 - 50 of 147 citations for Transglutaminase Substrate biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The SNAP tag on Nup358min-zip and Nup358min was biotinylated with SNAP-biotin substrate (New England BioLabs, MA) as described99.
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bioRxiv - Biophysics 2021Quote: ... 3’-biotinylated λ-DNA (NEB, Ipswich, MA) was attached to the pre-added lipid bilayer surface (DOPC ...
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bioRxiv - Biophysics 2019Quote: ... fusion proteins were directly biotinylated with BG-Biotin (NEB), buffer exchanged with a Zeba Desalting Column (ThermoFisher ...
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bioRxiv - Genomics 2021Quote: ... biotinylated DNA fragments were purified by streptavidin beads (NEB). In step 6 ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were separated on magnetic streptavidin resin (NEB) and eluted with 2× SDS loading buffer supplemented with 4% SDS and 10 mM Β-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... CLIP-Cell TMR star substrate or CLIP-Surface 488 substrate (NEB S9219 and S9232) was reacted with dCas9-CLIP proteins at 37°C for 60min ...
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bioRxiv - Genetics 2019Quote: ... Unligated-biotinylated ends were removed using T4 DNA Polymerase (NEB). DNA was purified one final time with two Zymogen DNA Clean and Concentrate-5 Kit columns per ligation reaction and eluted with 65 μL TE (130 μL total) ...
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bioRxiv - Plant Biology 2020Quote: ... 1.5 μg of biotinylated histone peptides were incubated with streptavidin beads (NEB) in binding buffer (50 mM Tris-HCl 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... 1 μg of the biotinylated lambda DNA is treated with Nt.BspQI (NEB) for 1 hour at 50°C then heat inactivated for 20 minutes at 80°C ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting 5’ biotinylated PCR products were digested with BsaI-HF (NEB) to generate four base-pair overhangs ...
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bioRxiv - Microbiology 2020Quote: ... The TMR-Star substrate (New England Biolabs) was added at 250 nM ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescent cell permeable Snap-Cell substrates (NEB) were used in the following concentrations ...
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bioRxiv - Biophysics 2020Quote: Gaussia and Cypridina substrates were obtained from NEB or its original supplier ...
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bioRxiv - Microbiology 2021Quote: ... or 100 nM TMR substrate for SNAPtag (NEB) for 30 minutes at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... in vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer with 5 μg RNA substrate (1.8 knt. Same substrate of MTase-Glo experiment) and 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA), 1 μM methyltransferase or no enzyme as a negative control ...
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bioRxiv - Molecular Biology 2019Quote: ... The substrates were amplified using Phusion Polymerase PCR (NEB). The linear double-stranded molecules were denatured and renatured to produce both the parental linear molecules and a circle with nicks on each strand nearly half-way around the circle which were then ligated by Taq Ligase (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.3 µM fluorescent SNAP substrate (TMR Star, NEB, S9105) was added along with the primary antibodies
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bioRxiv - Microbiology 2021Quote: ... or 100 nM TMR substrate for SNAPtag (S9105S, NEB), for 30 min anaerobically ...
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bioRxiv - Cell Biology 2023Quote: ... SNAP-Cell 647-SiR substrate (S9102S New England Biolabs) was used to reveal SNAP-tag following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... a set of 5’-end biotinylated anti-sense DNA oligoes and 5ul RNase inhibitor (NEB) were added to the lysate ...
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bioRxiv - Biophysics 2022Quote: Nucleosome reconstitution was performed by salt dialysis on biotinylated DNA (pKYB1 vector; New England Biolabs) without any nucleosome positioning sequence ...
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bioRxiv - Developmental Biology 2022Quote: ... The biotinylated DNA was ligated in a reaction mixture containing 1x ligation buffer (NEB, B0202), a final concentration of 1% Triton X-100 ...
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bioRxiv - Genomics 2021Quote: ... Selected biotinylated DNA fragments ranging from 200-500bp were then ligated with illumina adaptors (NEB). The libraries obtained from biological replicates were multiplexed and further sequenced at Oxford Genomics Centre and Edinburgh Genomics (Genepool ...
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bioRxiv - Systems Biology 2023Quote: ... Biotinylated oligo tags were amplified on-bead using 2X Q5 Hot-Start Mastermix (NEB #M0494) with primers that add the indexed full Illumina adaptor sequences.
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bioRxiv - Plant Biology 2023Quote: ... Total mRNAs were isolated using biotinylated oligo(dT) and streptavidin magnetic beads (New England Biolabs) as previously described (Wang et al ...
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bioRxiv - Microbiology 2019Quote: ... Signal was detected with Clarity™ ECL Blotting Substrate (NEB) and on a GE Imagequant LAS 4000 mini ...
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bioRxiv - Biophysics 2020Quote: ... for the substrate-binding domain mutants and βamHI-AlwnI (NEB) for the transmembrane domain mutants (Appendix) ...
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bioRxiv - Molecular Biology 2023Quote: ... or ΦX174 RF I DNA (dsDNA substrate, NEB, Ipswich MA) into buffer containing 50 mM Bis-Tris pH 6.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Surface biotinylated proteins immobilized on streptavidin agarose beads were denatured in Denaturing Buffer (New England Biolabs) at 100°C for 10 min in accordance with the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... After 24 hours SNAP-Cell 647-SiR substrate (New England Biolabs) was added to the medium at a concentration of 3 μM ...
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bioRxiv - Biochemistry 2020Quote: Translocation substrates were synthesized in vitro using the PURExpress system (NEB) in the presence of [35S]-methionine for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... the substrates used are 5’-end-labeled with T4 PNK (NEB) in the presence of gamma 32P-ATP ...
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bioRxiv - Microbiology 2019Quote: ... The double-stranded DNA substrate pUC19 (New England Biolabs, MA, USA) was linearized by digesting with PstI (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Circular ssDNA (M13MP18) substrate (0.5 µg, New England Biolabs, MA, USA) was combined with 4 pmol of each cellular nuclease alone and in combination ...
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bioRxiv - Cell Biology 2019Quote: ... cells were labeled with Oregon-Green SNAP substrate (New England Biolabs) at 4 µM final concentration for labeling of the newly synthesized CENP-A molecules ...
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bioRxiv - Biochemistry 2021Quote: ... the pre-crRNA substrates were 5′-radiolabeled with T4 PNK (NEB) in the presence of gamma 32P-ATP ...
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bioRxiv - Biochemistry 2023Quote: ... The dsDNA substrate was a ~10-kb Nt.BbvCI-nicked (NEB #R0632) pMYC plasmid used at 2.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the dsDNA substrate was a ~10-kb Nt.BbvCI-nicked (NEB #R0632) pMYC plasmid used at 5 ng/µL (~0.83 nM) ...
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bioRxiv - Genetics 2019Quote: ... ends were filled in with dNTPs and biotinylated dCTP at 0.4 mM concentration using Klenow Exo-(NEB) for 1 hr at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... 500 ng biotinylated λ DNA was incubated at 37°C with 1.6 M S-adenosylmethionine (New England BioLabs) and 20 U CpG methyltransferase M.SssI (New England BioLabs ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by on-bead 5’ ligation of a biotinylated REL5 linker sequence using T4 RNA ligase 1 (NEB) for 3 h at 37⁰C ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated antibody binding was detected using TMB substrate (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: 5’-end 32P-labelled DNA substrates were generated using T4 PNK (NEB) and [ɣ-32P]ATP (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... DNA substrates were 5’ terminally labelled with T4 Polynucleotide Kinase (NEB, M0201S) and ATP-γ-32P (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: ... recombinant HAΔTM H1N1 A/Michigan/45/2015 and H3ΔTM H3N2 A/Hong Kong/4801/2014 were biotinylated and coupled to streptavidin magnetic beads (New England Biolabs) at a ratio of 2.5 μg of protein to 25 μl of beads ...
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bioRxiv - Biochemistry 2020Quote: ... The substrate was then treated with 50 U of uracil DNA glycosylase (NEB) for 2 h at 37°C to form the abasic site ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 3 times with PBS-T and TMB substrate (Denovo Biolabs) was added for development of color ...
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bioRxiv - Biophysics 2021Quote: ... was filled in with a mixture of natural and biotinylated nucleotides by the exonuclease-deficient DNA polymerase I Klenow fragment (New England BioLabs). Reaction was conducted by incubating 10 nM λ-DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Ribodepleted RNA were then ligated to 10 pmol of a biotinylated 3’ adapter, (3’-Adap TAIL-seq, Supplementary Table 7) using 10 units of T4 RNA ligase 1 (NEB) in a final volume of 10 μl for one h at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... the 12-base 5’ overhang on each end was filled in with a mixture of unmodified and biotinylated nucleotides by the exonuclease-deficient DNA polymerase I Klenow fragment (New England BioLabs). The reaction was conducted by incubating 10 nM λARS1 DNA ...