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Citations for New England Biolabs :
1 - 50 of 132 citations for Sulfo NHS LC Biotin sodium since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... was biotinylated with sulfosuccinimidyl-6-[biotinamido]-6-hexanamido hexanoate (sulfo-NHS-LC-LC biotin; ThermoScientific) and coupled to streptavidin beads (New England Biolabs). Patient samples were incubated with RBD-coupled beads and excess sera washed off with PBS (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: Benzyl-guanine NHS ester (BG-GLA-NHS; NEB S2040) was covalently linked to the C7-amine modified oligonucleotide (Sigma) ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... benzyl-guanine NHS ester (BG-GLA-NHS; New England Biolabs, Ipswich, MA) was covalently linked to C6-amine oligonucleotides (with or without Cy3 modification ...
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bioRxiv - Biophysics 2024Quote: ... R4: 5’-TAGGTACAGTCCTCAGGTGTCTT[AmC6dT]T) were coupled with SNAP-substrate NHS ester (BG-GLA-NHS, NEB). The DNA strands at 1 mM concentration were incubated with 10 mM of BG-GLA-NHS and reactions were cleaned up using a Bio-Spin P-30 column (Bio-rad).
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bioRxiv - Synthetic Biology 2021Quote: ... BG-GLA-NHS (New England BioLabs) was dissolved in DMSO at 20 mM ...
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bioRxiv - Biophysics 2020Quote: ... and 3-biotin-GTP (NEB) and purified using MEGAclear ...
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bioRxiv - Biophysics 2021Quote: CoA-biotin (New England Biolabs) was coupled to the ybbR-tag of the VWF-dimer constructs in a bulk reaction in the presence of 5 μM sfp phosphopantetheinyl transferase53 and 10 mM MgCl2 at 37 °C for 60 min ...
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bioRxiv - Biophysics 2020Quote: CoA-biotin (New England Biolabs) was coupled to the ybbR-tag at the C-terminus of the fusion protein constructs in a 90 min bulk reaction in the presence of 4 µM sfp phosphopantetheinyl transferase63 and 100 mM MgCl2 at room temperature (≈ 22°C) ...
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bioRxiv - Cell Biology 2023Quote: ... BG-GLA- NHS (New England Biolabs, Ref. S0151S), ciliobrevin D (CBD ...
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bioRxiv - Biophysics 2023Quote: Biotin-SNAP-mDia1 prepared by incubating purified SNAP-mDia1 with Benzylguanine-Biotin (New England Biolabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... Synthesis was accomplished with excess BG-GLA-NHS (NEB) in PBS pH 7.4 for 1 hr at room temperature and buffer exchanged on a Zeba 7k MWCO desalting column ...
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bioRxiv - Biophysics 2020Quote: ... the biotin-Chd1p was prepared by mixing the SNAP-Chd1p and SNAP-biotin® (New England BioLabs) in a 1:2 molar ratio and incubated at room temperature for 30min ...
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bioRxiv - Immunology 2020Quote: ... or 8 μM bortezomib (BTZ; LC Biolabs). Mock treatment consisted of an equivalent volume of the matching solvent ...
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bioRxiv - Genetics 2020Quote: ... sodium orthovanadate (NEB, 1mM), and sodium fluoride (NEB ...
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bioRxiv - Physiology 2022Quote: ... sodium orthovanadate (NEB, 1mM), sodium fluoride (NEB ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 μL of 10 mM biotin (NEB) was then added to each sample on the plate and incubated for an additional five minutes to compete away purely biotin-dependent interactions ...
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bioRxiv - Biophysics 2019Quote: ... with 2 μl of 20 mM BG-GLA-NHS (NEB) in 20 μl 100 mM sodium borate buffer ...
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bioRxiv - Genomics 2021Quote: DNA ends were marked with biotin-14-dATP by adding 60μL of biotin fill-in master mix (1X NEB 3.1 ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 1 mM BG-biotin or BG-(PEG)12-biotin (New England Biolabs; PEG linker available on request) was added to 200 μl of the purified axonemes in HMEEK buffer and incubated overnight at 4 °C ...
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bioRxiv - Systems Biology 2021Quote: ... the purified DNAs were annealed using random nonamer primers with a 5′-biotin tag (5′-Biotin-CTACACGACGCTCTTCCGATCTNNNNNNNNN-3′) in the presence of Klenow fragments (3′-5′ exo-, New England Biolabs). Then ...
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bioRxiv - Physiology 2022Quote: ... sodium fluoride (NEB, 1 mM), and sodium butyrate (100uM) ...
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bioRxiv - Synthetic Biology 2020Quote: ... BG-fluorescein or BG-biotin conjugates (known as “SNAP-Surface® 649”, “SNAP-Cell® Fluorescein” and “SNAP-Biotin” respectively, NEB, at a 1:500 dilution ...
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bioRxiv - Genetics 2023Quote: ... using a biotin-labeled 30 μM dNTP mix (dATP, dGTP, dTTP and Biotin-14-dCTP Thermo Fischer, 19518018) and Klenow enzyme (NEB, M0210L) at 37°C for 80 min ...
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bioRxiv - Genomics 2020Quote: ... Biotin labeled dATP (Thermo,19524016) and Klenow (NEB, M0210) were used to fill restriction fragment overhangs ...
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bioRxiv - Biophysics 2019Quote: ... fusion proteins were directly biotinylated with BG-Biotin (NEB), buffer exchanged with a Zeba Desalting Column (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mM Vanadate (Sodium Orthovanadate, NEB, pre-incubated for ten minutes at 95 °C to dissociate Vanadate oligomers ...
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bioRxiv - Genetics 2020Quote: ... and sodium fluoride (NEB, 1 mM). 150uL of ice cold buffer was added to heads followed by immediate homogenization with a motorized pestle for 10 seconds on ice ...
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bioRxiv - Cell Biology 2019Quote: ... and 10mM sodium orthovanadate (NEB, P0758S). Samples were subsequently subjected to in vitro kinase assay (in vitro samples ...
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bioRxiv - Cell Biology 2019Quote: ... and 10mM sodium orthovanadate (NEB, P0758S), and incubated for 30 minutes/30°C ...
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bioRxiv - Bioengineering 2024Quote: ... Then it was incubated with 20ME (Molar equivalent) of BG-GLA-NHS (NEB) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... or sodium orthovanadate (Na3VO4, 1 mM; NEB) to test dephosphorylation of CatSper1 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 5 mM sodium orthovanadate (New England Biolabs), and 10 mM sodium fluoride (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... containing 1 mM sodium orthovanadate (NEB, # P0758S). The membrane from the transwell inserts was cut using forceps and placed in 300 µL of 1X cell lysis buffer (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... N-hydroxysuccinimide ester (BG-GLA-NHS) functionalized benzylguanine was purchased from NEB (Cat #S9151S) and freshly reconstituted in DMSO to a final concentration of 83 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... were then combined with 10 μM CLIP-Biotin (New England Biolabs) and rotated (end-over-end ...
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bioRxiv - Genomics 2019Quote: ... Biotin labelled DNA was pulled down on Streptavidin Dynabeads (NEB, S1420S). After DNA repair and 3’ A addition ...
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bioRxiv - Genomics 2019Quote: ... Biotin labelled DNA was pulled-down on Streptavidin Dynabeads (NEB, S1420S). After DNA repair and 3’ A addition ...
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bioRxiv - Genomics 2023Quote: ... dTTP and biotin-dATP using Klenow DNA polymerase I (NEB M0212L), followed by in situ proximity ligation at room temperature overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Sodium Orthovanadate (New England Biolabs, Cat. no. p0758s), Pepstatin (Cat ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 0.5% sodium dodecyl sulfate) and Proteinase K (NEB). Reactions were incubated at room temperature overnight ...
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bioRxiv - Biophysics 2021Quote: ... The BG-oligonucleotide was prepared by coupling of 10 mM BG–GLA–NHS (S9151, NEB) to 0.33 mM amine-functionalized oligonucleotide (GGGCGGCGACCT-NH2 ...
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bioRxiv - Cell Biology 2022Quote: ... 250 μM of each oligo was incubated with 13.28 mM of BG-GLA-NHS (NEB) in 100 mM sodium borate and 50% v/v DMSO ...
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bioRxiv - Biophysics 2022Quote: ... rk430-mScarlet-SNAP (7 μM monomer) was incubated with benzylguanine-biotin (NEB) in a 4 to 1 molar ratio at room temperature for 15 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... Antibodies were then co-incubated with a 20-fold molar excess of BG-GLA-NHS (NEB) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Sodium Orthovanadate (Vanadate) was from New England BioLabs (NEB), catalase and hydrogen peroxide (30 % ...
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bioRxiv - Biophysics 2021Quote: ... and Sodium Orthovanadate (New England BioLabs, Ipswich, MA, USA). Tissue was homogenized using an electric homogenizer ...
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bioRxiv - Synthetic Biology 2020Quote: ... SNAP reagents (BG-649, BG-fluorescein and BG-biotin) were obtained from NEB. Electrocompetent cells were used in all transformations.
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bioRxiv - Cell Biology 2021Quote: ... and biotin-pupE labelled proteins were enriched by streptavidin magnetic beads (NEB, S1420S) and identified by mass spectrometry analysis (Liu et al. ...
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bioRxiv - Biophysics 2023Quote: ... The biotin-modified DNA was digested using a KasI restriction enzyme (NEB #R0544S) that produced two fragments of 24 kb and 24.5 kb ...
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bioRxiv - Genomics 2020Quote: ... 0.5% Sodium deoxycholate and 1.1% Murine Rnase Inhibitor (M0214L, NEB). Cells were allowed to lyse for 15 min on ice ...