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Citations for New England Biolabs :
1 - 50 of 1708 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... each culture was recovered in provided outgrowth media (NEB) by shaking at 37°C at 250 rpm for 1.5 hours ...
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bioRxiv - Bioengineering 2019Quote: ... the culture media mix was TSB with 1 mg/mL BSA (New England Biolabs) and 50 μM calcein ...
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bioRxiv - Cell Biology 2019Quote: ... the HeLa cells were kept in HeLa culture media treated overnight with 0.2 U/mL of □2-3,6,8,9 Neuraminidase (New England Biolabs) to cleave sialic acid from the cell surface glycans.
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples collected from culture media were treated with PNGase F enzyme (New England Biolabs, P0709S) to remove the N-linked oligosaccharides from the TECTA protein according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with media containing 3 µM SNAP-Cell block (New England BioLabs cat. # S9106S) and cells maintained at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μM kifunensine was added to the ExpiCHO culture media at the time of transfection and Endo Hf (NEB) treatment of LC-kappa purified protein (∼10,000 U/mg protein at 4°C for 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... The cultures were monitored using the Cell Growth Quantifier (Aquila Biolabs) with readings every 60 s and incubated at 37 °C and 210 rpm until the late stationary phase was reached ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were labelled in full media with SNAP-Surface 549 (New England Biolabs) for 30 minutes at 37°C and imaged in RPMI without phenol red in a Nikon Eclipse Ti spinning disk confocal microscope with an ORCA-Flash 4.0 camera (Hamamatsu ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated from the cell culture supernatants and converted to cDNA (NEB). The E gene of stock virus ...
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bioRxiv - Bioengineering 2020Quote: ... Electroporated cells were immediately transferred into pre-warmed SOC recovery media (New England Biolabs) and recovered for 1-2 hours at 37 °C with 250 rpm of shaking ...
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bioRxiv - Microbiology 2019Quote: ... cells recovered for three hours at 37°C in SOC media (NEB, cat. # B9020S). An aliquot was then taken to titer transformants and the remaining cells (860 µl ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 30 min at 37°C in appropriate media containing 3µM of cell-permeable SNAP-Cell® 647-SiR (New England BioLabs). Cells were then rinsed twice with fresh media and incubated for 30min at 37°C ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from 0.25×106 cells at day 12 of cell culture using the Monarch total RNA miniprep kit (New England Biolabs, T2010S) and quantified using the Qubit RNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Deglycosylated cell lysates or conditioned media were prepared by treatment of PNGase F (New England Biolabs). Samples containing around 30 μg proteins was loaded to SDS-PAGE ...
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bioRxiv - Plant Biology 2023Quote: ... the culture filtrates from methanol-induced cultures were treated with PNGaseF (New England Biolabs), and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis ...
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bioRxiv - Biophysics 2021Quote: ... 5% CO2 -balanced complete cell culture medium with 5 μM SNAP-Surface Alexa Fluor 647 (NEB, S9136S), for 15 minutes at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... the bank vole lung homogenate and Mygla.REC.B cell culture supernatant were treated with a cocktail of micrococcal nuclease (New England BioLabs) and benzonase (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... gibco) supplemented with 10% fetal calf serum (eurobio scientific) and 1% ZellShield cell culture contamination preventive (Minerva-Biolabs). Cells were passaged at 80-90% confluency ...
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bioRxiv - Neuroscience 2020Quote: ... The holder allowed immersion of the cochlea and the middle ear in oxygenated (95 % O2, 5 % CO2) cell culture medium (Minimum Essential Medium with Earle’s balanced salts, SH30244.FS Nordic Biolabs). The bone of the bulla was removed gently with bone cutters which exposed the middle ear and the basal turn of the cochlea ...
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bioRxiv - Microbiology 2020Quote: ... TMR-Star was added to cells in culture samples inside an anaerobic chamber to a final concentration of 250 nM (New England Biolabs) and the mixture incubated for 30 minutes in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... Thymidine was then removed by washing cells with culture media and blocking of existing CENP-A was performed by treatment with 10 mM SNAP-Cell® Block BTP (S9106S, NEB) for 30 min ...
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bioRxiv - Microbiology 2020Quote: Mutations within the stem-loop binding site of SHOxi were constructed using the Q5 Site-directed Mutagenesis kit (NEB) standard protocol on the previously described SHOxi overexpression construct (in pTA1300) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of Universal nuclease (Pierce; 125U/L of culture) and 10 μl of DNaseI (NEB; 20U/L of culture) were added and the mixture allowed to incubate for 10 min at RT ...
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bioRxiv - Genomics 2020Quote: ... Cultures were infected with 8ml M13K07 helper (NEB) for 60min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 50 mL cell culture were grown in LB to stationary phase and processed with the NEB Monarch genomic DNA purification kit (NEB #T3010). For genomic DNA extraction of E ...
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bioRxiv - Synthetic Biology 2020Quote: ... and media were obtained from New England Biolabs (NEB) unless otherwise noted ...
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bioRxiv - Genetics 2021Quote: ... Plasmid was isolated from the remainder of the original 5-mL culture of the R599A culture using standard methods and digested with PvuI-HF (New England Biolabs #R3151) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by annealing and ligation with a DNA/RNA-hybrid stem-loop adapter using T4 RNA ligase 2 (New England Biolabs), which specifically ligates the adapter to mature tRNA ...
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bioRxiv - Genetics 2020Quote: ... containing the silent mutations to stem 2 of SARS and SARS-CoV2 (IDT) into the plasmid using T4 DNA ligase (NEB).
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bioRxiv - Biophysics 2022Quote: ... 100 uL of room temperature SOC media (New England Biolabs) was added to the cells ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 500ng of RNA (tissue samples) or 100ng RNA (cell culture experiments) using the LunaScript RT Supermix kit (New England Biolabs, Cat. No. E3010L) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2021Quote: ... xanthus cultures using the Monarch Total RNA Miniprep Kit (NEB). 109 cells were harvested and resuspended in 200 µl lysis-buffer (100 mM Tris-HCl pH 7.6 ...
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bioRxiv - Genomics 2020Quote: ... Plasmid DNA was purified from liquid cultures using DNA miniprep kit (NEB #T1010).
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bioRxiv - Microbiology 2022Quote: DNA was extracted from single-colony cultures (Monarch Genomic DNA Purification Kit, NEB). Short read libraries were prepared based on the NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... untreated cultures of cortical neurons (DIV8) were lysed in lambda phosphatase buffer (NEB), incubated for 20 min to ensure total lysis ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants (150 μL) were treated with DNase I (NEB, Ipswich, MA, USA) to obtain only enveloped and encapsidated viral genomes ...
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bioRxiv - Microbiology 2023Quote: ... coli overnight cultures using a Monarch plasmid miniprep kit (New England Biolabs, USA) and concentration was quantified by NanoDrop (Thermo Scientific ...
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bioRxiv - Genetics 2021Quote: MBP-Stwl antigen was purified from induced bacterial culture using Amylose Resin (NEB: E8021L). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... Samples of E.coli cultures were harvested and resuspended in E.coli lysis reagent (NEB, US). Lysates and purified proteins were separated by 4-12% Bis-Tris SDS-PAGE (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... DNA extracted from the 30mL culture was digested with HindIII-HF (#R3104S, New England Biolabs) and BamH1-HF (#R3136S ...
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bioRxiv - Microbiology 2023Quote: Turicibacter genes were amplified from template culture lysates with Phusion or Q5 DNA polymerase (NEB) and primers designed to amplify denoted Turicibacter genes ...
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA were extracted from bacterial cultures using Monarch Plasmid Miniprep Kit (New England Biolabs) and sequenced using the BigDye Terminator v.1.1 kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... This was added to 5 ml (per 2 L of culture) amylose resin (New England Biolabs) equilibrated in lysis buffer and left on a tube roller shaker at 4 °C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids from O/N cultures were extracted with the Monarch Plasmid Miniprep Kit (New England Biolabs) and sent for sequencing to LGC genomics.
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bioRxiv - Molecular Biology 2022Quote: ... was added to the culture prior to RNA extraction using Monarch total RNA miniprep kit (NEB). Isolated RNA was quantified using Quibit RNA BR assay kit (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... the plasmid was isolated from the dense culture using the Monarch® Plasmid Miniprep Kit (NEB) and the DNA was eluted with nuclease-free water ...
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bioRxiv - Systems Biology 2023Quote: ... we pelleted each liquid culture by centrifugation and used the Monarch Plasmid Miniprep Kit (NEB #T1010L) to isolate plasmid according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Lysates (20 μg) and media immunoprecipitates were diluted to 10 μL in 2X glycoprotein denaturing buffer (NEB), and boiled for 10 min ...