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Citations for New England Biolabs :
1 - 50 of 8862 citations for SpectraDye Antibody Labeling Kit 350 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The protein was labeled with SNAP-Surface Alexa Fluor 647 labeling kit (New England Biolabs) according to the protocol provided by the manufacturer ...
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bioRxiv - Genomics 2023Quote: ... 350 U of DpnII 50U/µl (NEB cat. R0543M) in 300 µl 1x NEBuffer DpnII (NEB cat ...
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bioRxiv - Genomics 2019Quote: ... and then used for 350-bp paired-end library construction with the Next Ultra DNA library prep kit (NEB) for Illumina sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then mixed with 350 μl 1× RNA protection reagent and RNA was purified using a Monarch total RNA extraction kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: The template genomic DNA was fragmented by sonication to an insert size of 350 bp using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample and sequenced using an Illumina Hiseq 2500 PE150.
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bioRxiv - Biophysics 2023Quote: ... Following mutagenesis for fluorescent labeling and/or creating RTT mutations using the Q5 mutagenesis kit (New England BioLabs), plasmids were transformed into E ...
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bioRxiv - Genomics 2020Quote: ... A cDNA library with insert sizes of 350-400 bp was prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, MA, USA) and paired-end sequenced on the HiSeq X ten platform (Illumina Inc. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... dual-indexed Illumina genomic libraries were prepared for each DNA sample using an insert size of ~350 bp and the Ultra II Library Prep Kit (New England BioLabs, Ipswich, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... or without 350 units of recombinant GSK3β (rabbit skeletal muscle) (New England BioLabs) and 1X hot kinase buffer (50mM Tris ...
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bioRxiv - Genomics 2019Quote: ... Two strand-specific RNA-seq libraries with an insert size of 350 bp using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) were prepared and sequenced on an Illumina NovaSeq 6000 platform ...
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bioRxiv - Biochemistry 2021Quote: ... using a standard T4 polynucleotide kinase labeling protocol (M0201L, NEB). Each binding reaction (10 μL sample volume ...
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bioRxiv - Biophysics 2022Quote: ... Three are commercially available orthogonal self-labeling approaches: SNAP-tag (NEB), CLIP-tag (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... For nucleosomes labeling reaction mix contained: NEBuffer™ 2 (NEB B7202), protease and HDAC inhibitors (as detailed above) ...
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bioRxiv - Molecular Biology 2019Quote: ... Capping and cap-labeling were performed with the Vaccinia Capping System (NEB). To generate unlabeled capped target ...
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bioRxiv - Biochemistry 2020Quote: ... by SDS-PAGE after labeling with SNAP-Surface 549 (New England Biolabs). The strains had doubling times similar to their parental strains YF702 or YF4 (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... an identical labeling scheme was followed using CLIPcell block (10 µM; NEB) and CLIPcell-TMR (1 µM ...
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bioRxiv - Cancer Biology 2024Quote: ... Nucleosomes labeling the following reaction was used: NEBuffer™ 2 (NEB B7202), inhibitors (as detailed above ...
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bioRxiv - Cell Biology 2022Quote: ... 350 ng Dolcetto setA library pool was used to transform 100 μl electrocompetent bacteria (NEB, C3020K), split into 4 × 25 μl electroporations ...
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bioRxiv - Synthetic Biology 2019Quote: ... was accomplished by end-labeling oligos with TdT (New England Biolabs, Ipswich, MA) and fluorescein-12-ddUTP (Perkin Elmer ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 µL of labeling mix (4 µL 10X ThermoPol Reaction buffer (NEB, B9004S), 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP ...
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bioRxiv - Biochemistry 2019Quote: ... RNA primer end-labeling was accomplished using T4 polynucleotide kinase (T4 PNK, NEB) and 25 μCi of ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... and end labeling was performed using T4 PNK from NEB (cat number: M0201S) and biotin-14-dATP from Invitrogen (cat number ...
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bioRxiv - Cell Biology 2023Quote: ... 100 nM okadaic acid (Enzo LifeSciences, ALX-350-011-M001) 50 U micrococcal nuclease (New England Biolabs, M0247S)) ...
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bioRxiv - Cancer Biology 2023Quote: ... 350 ng of total RNA were used for mRNA enrichment using NEBNext mRNA polyA isolation module (NEB, #E7490L) and libraries for Illumina sequencing were prepared using the NEBNext Ultra II RNA kit (NEB ...
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bioRxiv - Genetics 2021Quote: ... Radioactive end-labeling was performed with the addition of T4 PNK (20 Units, NEB) and 2 μl [γ-32P]ATP (6000Ci/mmol ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by labeling with γ-P32-ATP and T4 polynucleotide kinase (Cat#: M0201L, NEB). The reactions were further processed and digested with P1 Nuclease (Cat# ...
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bioRxiv - Molecular Biology 2020Quote: ... 8 μl 5x PNK pH 6.5 buffer [350 mM Tris-HCl pH 6.5, 50 mM MgCl2, 5 mM DTT], 1 μl PNK enzyme [NEB] ...
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bioRxiv - Microbiology 2022Quote: ... SNAP labeling before live-cell imaging with SNAP-Cell 647-SIR (New England Biolabs GmbH) was done according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer ...
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bioRxiv - Neuroscience 2023Quote: ... TCF/LEF family antibody sampler kit (New England BioLabs), NFAT1 (New England BioLabs) ...
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bioRxiv - Neuroscience 2024Quote: ... 350 ng of total RNA were used for mRNA enrichment using NEBNext mRNA polyA isolation module (NEB, Ornat, Rehovot, Israel, #E7490L) and libraries for Illumina sequencing were prepared using the NEBNext Ultra II RNA kit (NEB ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were subjected to a methylation labeling reaction using a solution of 1x M.CviPI Reaction Buffer (NEB), 300 mM sucrose ...
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bioRxiv - Molecular Biology 2021Quote: ... Table 4 was end labeled using gamma-ATP and T4 Polynucleotide Kinase radioactive labeling protocol from NEB. Labelled oligos were purified using GE Healthcare illustra ProbeQuant G-50 Micro Columns and the membrane was probed overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... gel purified and transcribed with DIG RNA labeling mix with either T7 RNA polymerase (NEB, M0251S; antisense probe), or SP6 RNA polymerase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Specific probes were generated by end-labeling of oligodeoxyribonucleotides (Table S3) using T4 Polynucleotide Kinase (New England Biolabs) and [γ-32P]-ATP (Hartmann Analytic) ...
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bioRxiv - Genomics 2019Quote: ... DNA was sheared to ~350 bp mean fragment size and sequencing libraries were generated using NEBNext Ultra enzymes (New England Biolabs, Ipswich, MA, USA) and Illumina-compatible adapters ...
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bioRxiv - Microbiology 2019Quote: ... Each RNA was subjected to radioactive end-labeling or non-radioactive phosphorylation by T4 Polynucleotide Kinase (New England Biolabs). Each RNA was subjected to phosphorylation with equimolar concentrations of either 32P-γ-ATP or ATP ...
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bioRxiv - Genetics 2020Quote: ... Fluorescent open chromatin DNA labeling was performed by incubating the nuclei in presence of 2.5 U of Nt.CviPII (NEB R0626S), 50 U of DNA polymerase I (M0209S ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasmids were linearized with appropriate restriction enzymes and cRNA preparation was carried out using Roche DIG-labeling reagents and RNA polymerases (NEB). We used previously published probes for olig2 (H.-C ...
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bioRxiv - Microbiology 2022Quote: ... SNAP labeling with SNAP-Cell 647-SIR was done as described in the manual for SNAP-Cell 647-SIR (NEB). Afterward ...
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bioRxiv - Microbiology 2022Quote: ... SNAP labeling with SNAP-Cell 647-SIR was done as described in the manual for SNAP-Cell 647-SIR (NEB) before imaging ...
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bioRxiv - Biophysics 2022Quote: SNAP-tag labeling of SNAP-mGluR2 was done by incubating cells with 2 µM of SNAP-Surface Alexa Fluor 549 (NEB) and 2 µM of SNAP-Surface Alexa Fluor 647 (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... were manipulated in a final volume of 20 μL labeling mixture containing 1X T4 polynucleotide kinase (PNK) enzyme buffer (NEB), milli-Q water ...
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bioRxiv - Biophysics 2023Quote: ... 24 hrs post-transfection cells were prepared for experiments by labeling tem with 1 µM BG-Surface Alexa 546 (non-permeable dye, New England BioLabs) for 30 min at 37°C in a media containing (in mM) ...
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bioRxiv - Biochemistry 2019Quote: ... The SpCas9 variants designed for site-specific labeling were constructed by QuikChange site-directed mutagenesis with Q5® High-Fidelity DNA Polymerase (NEB) and confirmed by DNA sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... The specific isotope probe was generated by end-labeling DNA oligonucleotides complementary to the miR167a sequence with [γ-32P] ATP by T4 polynucleotide kinase (New England Biolabs). The level of U6 snRNAs was used as the internal control ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNA then was pulldown using m6A specific antibody using EpiMark N6-methyladenosine enrichment kit(NEB). RNA was converted into cDNA using random hexamer primers and Revert aid RT (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... m6A pulldown was performed with a rabbit monoclonal anti-m6A antibody in the EpiMark N6-Methyladenosine Enrichment Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragmented RNA was mixed with Protein G Magnetic bead prebound monoclonal anti-m6A antibody (1 µL) from the EpiMark N6-Methyladenosine Enrichment Kit (New England Biolabs), resuspended in 300 µl EpiMark IP buffer supplemented with murine RNase inhibitor (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer, 1 µL T4 PNK, NEB, 1 µL γ-32P-ATP, Hartmann, 6 µL ddH2O) for 40 min at 37°C ...