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Citations for New England Biolabs :
1 - 50 of 4812 citations for Somatostatin Receptor 1 SSTR1 Rabbit Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... purified by affinity chromatography (amylose resin (NEB) or Strep-Tactin XT resin (IBA) ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of SNAP-Surface Alexa Fluor 546 (1:1000, New England Biolabs) to DMEM F12 without serum and phenol red (21041-025 ...
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bioRxiv - Biochemistry 2021Quote: ... Fusion proteins were purified in batch by amylose affinity (NEB), eluting in buffer B (buffer A with 0.02% DDM ...
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bioRxiv - Biophysics 2022Quote: ... Fusion proteins were purified in batch by amylose affinity (NEB), eluting in buffer B (buffer A with 0.02% DDM ...
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bioRxiv - Microbiology 2020Quote: ... MBP-tagged RBD was affinity purified by Amylose Resin (NEB) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Fusion proteins were purified in batch by amylose affinity (NEB), eluting in buffer B (buffer A with 0.02% DDM ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-SNAP (1:1000, polyclonal, New England Biolabs, Ipswich, MA, P9310S), or mouse anti-tubulin (1:40,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Nsp1–40S complex was then affinity-purified using amylose resin (NEB), followed by size-exclusion chromatography ...
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bioRxiv - Bioengineering 2021Quote: ... Each His or MBP fusion protein was purified through affinity chromatography with TALON metal affinity resin (Clonetech, Palo Alto, CA) or with amylose resin (New England Biolabs), respectively.
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bioRxiv - Biochemistry 2020Quote: Expressed proteins were purified using the intein-mediated purification and affinity chitin-binding tag (IMPACT) chitin affinity matrix system (New England Biolabs). A cell pellet was dissolved in 8 mL of column buffer (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal anti-lactyllysine antibody (PTM Biolabs), or mouse monoclonal anti-β-actin antibody (A5316 ...
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bioRxiv - Molecular Biology 2020Quote: ... and was purified by affinity chromatography on amylose resin (NEB, Inc, USA) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... MBP-tagged proteins were purified by amylose affinity chromatography (New England Biolabs) following manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... and were first purified by affinity chromatography using the amylose resin (NEB). For untagged complex ...
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bioRxiv - Molecular Biology 2023Quote: ... Rabbit polyclonal anti-SNAP-tag (Cat no. P9310S, New England Biolabs, 1:1000 dilution). Secondary antibodies used were ...
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bioRxiv - Neuroscience 2022Quote: ... or SNAP-surface-Alexa488 (NEB) were added at the secondary antibody staining stage ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were affinity-purified using amylose resin using the manufacturer’s protocol (NEB) and eluted with 20 mM maltose ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was purified using chitin resin affinity purification (New England Biolabs, Inc.). hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was affinity purified using amylose resin (New England Biolabs, Ipswich, MA, USA). Wash buffer was 50 mM Tris pH 8.3 ...
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bioRxiv - Plant Biology 2021Quote: ... coli BL21(DE3) and purified by affinity chromatography using amylose resin (New England Biolabs). Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... The proteins were then purified by affinity chromatography using amylose resin (Catalog E8021L, NEB). The amylose resin was washed and pre-equilibrated in 20 mM tris ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal anti-SNAP-tag (New England BioLabs, P9310S), mouse monoclonal anti-HSP90 (BD Transduction Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... or a polyclonal rabbit antibody against cleaved casp-3 (1:100; New England Biolabs, Frankfurt, Germany), followed by a biotinylated goat anti-rabbit secondary antibody (Abcam ...
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Competition co-immunoprecipitation reveals interactors of the chloroplast CPN60 chaperonin machinerybioRxiv - Molecular Biology 2023Quote: ... The CPN60A construct was purified via Chitin-affinity chromatography according to the manufacturer’s instructions (NEB), hexa-histidine-tagged CPN20 was purified with nickel-affinity chromatography under denaturing conditions (Hammel et al. ...
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bioRxiv - Plant Biology 2023Quote: ... and affinity purified from Escherichia coli strain BL21 (DE3) using Amylose Resin (New England Biolabs) as previously described 5.
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bioRxiv - Biophysics 2023Quote: ... MBP-HNRNPH2 proteins were purified by affinity chromatography using amylose resin (#E8021, New England BioLabs) and eluted with buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 pmol of dephosphorylated and purified RNA was 5′ end-labeled (20 μCi of 32P-γATP) using 1 U of Polynucleotide Kinase (NEB) for 1 h at 37 °C in a 20 μL reaction volume ...
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bioRxiv - Neuroscience 2021Quote: ... We used rabbit polyclonal anti-lactyllysine antibody (PTM-1401, PTM Biolabs), mouse monoclonal anti-CaM Kinase II α subunit antibody (05-532 ...
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bioRxiv - Biochemistry 2023Quote: ... custom-made rabbit polyclonal anti-AcK142 or anti-AcK226 (Ez Biolabs) Abs were incubated overnight at 4 °C with the lysate from mock vs ...
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bioRxiv - Cell Biology 2021Quote: SNAP-GCGR was detected with an anti-SNAP-tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1/500) followed by goat anti-rabbit IgG H&L HRP (ab6271 ...
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bioRxiv - Plant Biology 2020Quote: ... The NRPD1-Maltose Binding Protein (MBP) fusion protein was affinity purified using Amylose resin (New England Biolabs) and eluted with 20 mM maltose ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was labeled with SNAP-Surface Alexa Fluor647 dye (New England Biolabs). The fractional dye labeling and DNA binding activity were assessed as previously described [12].
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bioRxiv - Plant Biology 2019Quote: ... Expression of recombinant proteins was performed at 25°C for 4 h with 0,3 mM Isopropil-β-D-1-tiogalattopiranoside (IPTG) and affinity purification was obtained by using the amylose resin affinity matrix (New England Biolabs) in a buffer containing 60 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was then further purified by a second affinity step over an 8 mL amylose column (NEB), where it was washed with 3 CV of Amylose Wash Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... 0.05% NP-40) and assembled complexes were affinity purified from the cushions using amylose resin (New England Biolabs). Following washing with buffer K-75 ...
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bioRxiv - Cell Biology 2022Quote: ... and isolated and affinity-purified using amylose resin according to the manufacturer’s protocol (New England Biolabs, Ipswich, USA). Polyclonal antisera were generated by immunization of 6-weeks old female NMRI mice (Charles River Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were probed with rabbit polyclonal anti-pansuccinyllysine antibody (PTM Biolabs, PTM-401) at a dilution of 1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Using 10 μg of custom-made rabbit polyclonal anti-AcK142 Ab (Ez Biolabs), AcK142 PNKP was IP’d from 1 mg of GO-treated chromatin fraction from WT-PNKP-FLAG and K142R-PNKP-FLAG expressing cells ...
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bioRxiv - Biophysics 2023Quote: ... Amylose affinity resin (NEB) was used for purification of fusion protein in batch which was eluted in buffer B (buffer A with 0.02% DDM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... expressed in BL21 cells with IPTG induction and affinity-purified on an amylose column using standard protocols (NEB #E8200S). Guinea pig reactive serum was produced by UC Davis Comparative Pathology Laboratory ...
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bioRxiv - Immunology 2022Quote: ... As an isotype control an unspecific polyclonal rabbit antibody was used (NEB, Frankfurt, Germany). To block ORF8 during differentiation it was preincubated with the polyclonal rabbit anti-ORF8 antibody or isotype over night and added to the monocytes (t=0 ...
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bioRxiv - Cell Biology 2020Quote: ... HsKinesin-II was purified using Ni column and MBP column successively (Ni-NTA agarose affinity resin, QIAGEN; Amylose resin, NEB New England Biolabs). For purification of Crkinesin-II ...
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bioRxiv - Biochemistry 2022Quote: ... His-MBP-tagged proteins were first purified by Ni-NTA affinity chromatography as above and then applied for 2 h to an amylose agarose matrix (NEB). Bound proteins were eluted with wash buffer containing 20 mM maltose ...
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bioRxiv - Microbiology 2020Quote: ... fusion protein was purified by affinity solid phase extraction on an amylose resin as suggested by the manufacturer (New England Biolabs). Rabbits were immunised by intramuscular injections of 400 μg purified MBP–A104R fusion protein every four weeks over a period of four months ...
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bioRxiv - Developmental Biology 2019Quote: ... The recombinant DBINO domain containing protein (~80kDa) was purified using the amylose affinity column (New England Biolabs, Massachusetts, United States) and detected using the anti-MBP-HRP antibody (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... into the pMAL-C2 vector and fusion proteins were expressed and purified using amylose-affinity chromatography as described by the manufacturer (New England Biolabs). The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... SNAP-GLP-1R and SNAP-GIPR were detected with an anti-SNAP-tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1/1,000) followed by goat anti-rabbit HRP secondary (ab6271 ...