Labshake search
Citations for New England Biolabs :
1 - 50 of 8855 citations for Soluble Starch Synthase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... SFP synthase (NEB), and Dy550-CoA (Thermo Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... SFP synthase (New England Biolabs) and purified ybbR-Luciferase-ybbR were incubated over night at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... SFP synthase was purchased from NEB. Methyl beta cyclodextrin was purchased from CycloLab ...
-
bioRxiv - Neuroscience 2022Quote: ... and Sfp synthase (New England Biolabs) in Tag-Lite Buffer for 1 hr at 30°C ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 µM ACP synthase (New England Biolabs), and 10 mM MgCl2 for 30 min at 37°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... nuclear soluble (NEB), chromatin-bound regions (ChrB) ...
-
bioRxiv - Molecular Biology 2021Quote: ... tropicalis pseudouridine synthase Pus7 was amplified from total RNA using the OneTaq One-step RT-PCR kit (New England Biolabs). XmaI and XhoI restriction sites were added with oligonucleotides and the resulting fragment was cloned into the p415Gal1 vector.
-
bioRxiv - Biophysics 2019Quote: ... using a 5-fold excess of fluorophore to protein and ∼1 μM Sfp synthase (NEB) for 16 hours at 6 °C in 50 mM TRIS-HCl pH 7.5 ...
-
bioRxiv - Immunology 2019Quote: ... The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.
-
bioRxiv - Biophysics 2019Quote: ... The soluble fraction was incubated with amylose resin (NEB) for 30 min at 4°C ...
-
bioRxiv - Biophysics 2024Quote: ... The soluble fraction was bound to amylose resin (NEB, USA) for 1 h and washed with 14 column volumes of wash buffer containing 50 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Cell Biology 2023Quote: ... The Oxiselect Comet Assay Kit (Cell-Biolabs) was used as a reference for the experiment ...
-
bioRxiv - Microbiology 2020Quote: ... The soluble proteins were mixed with amylose resin (New England Biolabs) according to the manufacturer’s protocols.
-
bioRxiv - Pathology 2021Quote: ... Soluble protein extracts were purified using amylose resin (New England Biolabs) and/or Ni-NTA agarose beads (Life Technologies).
-
bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was incubated with an amylose column (NEB E8021S) for two hours ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Soluble extract was obtained from supernatant and purified using amylose resin (NEB). The purified protein was frozen at −80 °C in in 20 mM Tris (pH 7.5) ...
-
bioRxiv - Biochemistry 2019Quote: ... The soluble fraction was gently mixed with amylose resin (New England Biolabs) for 30 min at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... CaMKAR was isolated from soluble lysate by nickel chromatography NEBExpress columns (NEB). Soluble protein was quantified by Pierce BCA assay (ThermoFisher ...
-
bioRxiv - Microbiology 2020Quote: ... the BioLux® Gaussia Luciferase Assay Kit (NEB, discontinued) or the GAR-2B Gaussia Luciferase Assay (Targeting Systems ...
-
bioRxiv - Molecular Biology 2023Quote: ... Non-soluble chromatin pellet was washed in 100 µL 1x MNase buffer (NEB), centrifuged and digested (2 min ...
-
bioRxiv - Cell Biology 2020Quote: ... or BioLux® Cypridina Luciferase Assay Kit (New England Biolabs) and Gaussia luciferase was assayed from 1:40 diluted cell culture supernatant using either the Stop & Glo reagent from the DualLuciferase® Reporter Assay System (Promega ...
-
bioRxiv - Cell Biology 2020Quote: ... or BioLux® Gaussia Luciferase Assay Kit (New England Biolabs) in a Lumat LB 9507 luminometer (Berthold Technologies).
-
bioRxiv - Microbiology 2021Quote: ... or a Biolux Gaussia luciferase assay kit (New England BioLabs) and a GloMax microplate reader (Promega) ...
-
bioRxiv - Biophysics 2020Quote: ... The soluble fraction was applied to 10 ml of chitin beads (New England Biolabs) equilibrated with chilled buffer A ...
-
bioRxiv - Biochemistry 2024Quote: ... Soluble protein fractions were mixed with an amylose resin column (New England BioLabs, Beverly, MA) pre-equilibrated with buffer A ...
-
bioRxiv - Biochemistry 2019Quote: ... we used a commercial assay kit (Ph.D.-7 Phage Display Peptide Library Kit, New England Biolabs) and followed the recommended protocol for “solution phase panning with affinity bead capture” with the following modifications ...
-
bioRxiv - Microbiology 2019Quote: ... Assays were performed using NEB BioLux Gaussia Luciferase (GLuc) kit (NEB #E3300L) following the stabilized protocol ...
-
bioRxiv - Microbiology 2024Quote: In vitro transcription/translation assays were conducted using the PURExpress kit (NEB) according to the manufacturer’s protocol with a 2-hour incubation at 37 °C ...
-
bioRxiv - Microbiology 2020Quote: The pcDNA3.1(-)-sACE2 plasmid expressing soluble ACE2 was made by Q5 site-directed mutagenesis (New England Biolabs) from the pcDNA3.1(-)-ACE2-Strep plasmid (Addgene ...
-
bioRxiv - Plant Biology 2022Quote: ... The soluble fraction of MBP-MP was applied to pre-equilibrated amylose agarose resin (New England Biolabs). After washing the column three times with amylose column buffer ...
-
bioRxiv - Molecular Biology 2023Quote: Coupled transcription-translation assays were carried out with the PURExpress kit (New England BioLabs) as described in (Lukasiewicz and Contreras ...
-
bioRxiv - Biochemistry 2023Quote: ... Assays were set-up using PURExpress in vitro protein synthesis kit (New England Biolabs) with a cloned full-length DHFR template ...
-
bioRxiv - Molecular Biology 2022Quote: RNA was purified by silica-based SPE using Monarch RNA Cleanup Kits (for in vitro assays) or Total RNA Miniprep Kit for intracellular samples (NEB).
-
bioRxiv - Molecular Biology 2021Quote: ... The soluble extract obtained by centrifugation was supplemented with 0.01% NP40 and incubated with amylose resin (New England Biolabs) at 4°C for 1 h ...
-
bioRxiv - Biochemistry 2022Quote: ... Expression of all soluble proteins used chemically competent home-made stocks of E.coli strain BL21(DE3) originally sourced commercially (NEB).
-
bioRxiv - Immunology 2021Quote: We quantified plasma luciferase activity by a BioLux Gaussia Luciferase Assay Kit (NEB, Ipswich, MA) from 10 μL plasma acquired by retro-orbital bleeding ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the High Sensitivity DNA Assay and NEBNext Library Quant Kit for Illumina (NEB, E7630). Finally ...
-
bioRxiv - Biochemistry 2019Quote: ... Precipitated proteins were removed by centrifugation at 14000g for 30 min at 4°C and the remaining soluble protein was incubated with enterokinase light chain (NEB) for 16h at room-temperature as per manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2019Quote: ... and the soluble fraction of the lysate was passed through a chitin bead column (New England Biolabs, Ipswich, MA, USA), allowing the CusB fusion to bind to the resin via its chitin-binding domain ...
-
bioRxiv - Biochemistry 2023Quote: ... after which the soluble supernatant was filtered (0.45 μm) and loaded onto a 42-mL column packed with amylose resin (NEB #E8021L) equilibrated with Buffer A3 (20mM Tris pH 7.5 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... GLuc activity in samples was measured using the Biolux Gaussia Luciferase Assay kit (NEB, Ipswich, MA). GLuc assay solution (50 μL ...
-
CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... WT SUMO3-CXCL17 and variants were expressed as soluble proteins in SHuffle® T7 Competent E.coli (New England Biolabs, Hitchin, UK). In both protocols ...
-
bioRxiv - Neuroscience 2019Quote: ... and 24 hours of incubation GLuc activity was quantified using the BioLux Gaussia Luciferase Assay Kit (NEB) according to the manufacturer’s instructions with luminescent detection on a SpectraMax M5 Microplate Reader (Molecular Devices ...
-
bioRxiv - Cell Biology 2020Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2019Quote: ... The assay was performed using the PURExpress In Vitro Protein Synthesis Kit (E6800S New England Biolabs, Inc.), following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using the Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB) with the CDC-derived primers for N1 and N2 gene targets and the reaction was performed using the QuantStudio™ 5 System (ThermoFisher) ...
-
bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
-
bioRxiv - Microbiology 2022Quote: ... while Gluc and Cluc expression levels were determined using Biolux Gaussia or Cypridina luciferase assay kits (New England BioLabs) and a microplate reader ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 pmol of TP_MAAAPQKCAAA* mRNA (see “Toeprinting assays” section) and components of the PURExpress ΔRibosomes Kit (New England Biolabs). The reaction was incubated at 37ºC for 20 min and then diluted in 50 mM Hepes KOH pH 7.5 ...