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Citations for New England Biolabs :
1 - 50 of 1037 citations for Slow Skeletal Troponin C TNNC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... were heated to 65°C and slow-cooled to 37°C before reverse transcription with 5 U AMV-RT (NEB, M0277L) at 42°C for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... Supplementary Table 1) were hybridized by slow cooling from 95-25°C and then phosphorylated using T4 Polynucleotide Kinase (NEB). The digested plasmid and the hybridized oligonucleotides were ligated using T4 Ligase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Table S4) were hybridised by slow cooling down from 95-25°C and then phosphorylated using the T4 Polynucleotide Kinase (NEB). The digested plasmid and the hybridised oligonucleotides were ligated using the T4 ligase (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... or without 350 units of recombinant GSK3β (rabbit skeletal muscle) (New England BioLabs) and 1X hot kinase buffer (50mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein blots were incubated with primary antibodies overnight at 4°C using the above-mentioned antibodies and the Gaussia Rabbit Polyclonal Antibody (E8023S, New England BioLabs), the Influenza A NS1 Mouse Monoclonal Antibody (sc-130568 ...
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bioRxiv - Neuroscience 2021Quote: ... C or E antibodies and the immunoprecipitates were treated with PNGase F (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... overnight at 4°C followed by Alexa Fluor 488 goat anti-rabbit secondary antibody (New England Biolabs, UK) for 1h ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibody rabbit anti-c-Fos (1:3000, New England Biolabs cat. No. 2250S) for 24 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and either stored at −20°C or 10 μg used for immunoblots with an Anti-IkBa antibody (New England Biolabs) (54).
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells were incubated at 37°C (30°C for NEB stable), 200 min-1 either in 5 ml (for a high copy ...
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bioRxiv - Genomics 2019Quote: ... the temperature was slowly ramped down (−0.1°C/sec) to 45°C (for Hybridase RNase H) or 22°C (for NEB RNase H), and the mixture was incubated at 45°C (for Hybridase RNase H ...
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bioRxiv - Microbiology 2022Quote: ... Around 100ng of the RNA was kept as input control and stored at -80°C whereas the remaining amount was subjected to immunoprecipitation using m6A specific antibody (NEB #E1610S). Initially ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Membranes were incubated overnight at 4 °C with the appropriate antibody: rabbit monoclonal anti-GSK3β (1:1,000; product # 9315, Cell Signalling Technology, New England BioLabs, Whitby, Ontario, Canada), rabbit polyclonal anti-p[Ser9]GSK3β (1:500 ...
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bioRxiv - Immunology 2021Quote: ... 60sec 72 °C (NEB # E7645S).
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bioRxiv - Neuroscience 2020Quote: ... and annealed (denaturation at 95°C for 5 min and then slow cooling of the samples at the rate of -2°C/sec from 95°C to 85°C and then - 0.1°C/sec from 85°C to 25°C for annealing of heteroduplexes) before addition of T7 Endonuclease I (NEB, Ipswich, Massachusetts, USA, M0302S). Heteroduplexes containing small mutations at the intended site should be cleaved into two fragments ...
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bioRxiv - Immunology 2021Quote: ... at 25 °C (Creative-Biolabs Inc.). RNA-1 conjugated with biotin (synthesized by IDT lnc. ...
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bioRxiv - Molecular Biology 2022Quote: ... LAMIN A/C – Nucleus (NEB #4777), SDHA – Mitochondria/Mitoplast (NEB #11998) ...
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bioRxiv - Plant Biology 2023Quote: ... and endoproteinase Lys-C (NEB-P8109S) at a ratio of (1:25 ...
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bioRxiv - Bioengineering 2023Quote: ... lysine-C (New England BioLabs, P8109S), protease and phosphatase inhibitor cocktail (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... annealed by temperature decrease from 95 °C to 4 °C and phosphorylated using T4 Polynucleotide Kinase (NEB, M0201) according to manufactures’ protocol ...
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bioRxiv - Systems Biology 2024Quote: ... at 16 °C overnight, followed by heat-inactivation (65°C, 15 min) and linearization using SbfI-HF (NEB) at 37°C for 2 h ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 30min at 75°C) and adapter ligation (NEB Quick ligase buffer 12.5µl ...
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bioRxiv - Neuroscience 2020Quote: ... incubated for 10 min at 30°C) or heat-inactivated (65°C for 20 min) as described in the supplier’s manual (New England Biolabs).
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bioRxiv - Genomics 2019Quote: ... and the mixture was incubated at 45°C (for Hybridase RNase H) or 22°C (for NEB RNase H) for additional 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... We incubated TXTL reactions at 29 °C for 6 h and digested at 37 °C with PacI (NEB R0547S) as instructed by the provider and added water instead of PacI for the undigested control ...
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bioRxiv - Cell Biology 2020Quote: ... at 68°C for 20 min and transferred to 42°C where 1.5 μL of ß-agarase (New England Biolabs) was added and incubated overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The lenti-sgRNA puro vector was digested with EcoRI for 2h at 37°C followed by digestion with BsmBI for 2 hours at 55°C and treatment with 4 μl of rSAP (NEB) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... cDNA synthesis was performed for 60 min at 42 °C and 5 min at 80 °C using ProtoScript II First Strand cDNA synthesis kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 95°C/15 s and annealing/extension: 65°C/5 min) of PCR using Q5 high-fidelity DNA polymerase (NEB). Amplicons were purified using 1× AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Immunology 2020Quote: ... 70°C for 10s and 72°C for 30s) and 1x 72°C for 5 min) using the Q5 polymerase (New England BioLabs) and cloned into pUC57 using the MEGAWHOP35 method ...
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bioRxiv - Genomics 2021Quote: ... 95°C/15 s and annealing/extension: 65°C/5 min) of PCR using Q5 high-fidelity DNA polymerase (NEB). Amplicons were purified using 1× AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2021Quote: ... 30 seconds at 65°C and 30 seconds at 72°C using the NEBNext® Multiplex Oligos kit (NEB, UK). The expected library size was ∼200-250bp ...
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bioRxiv - Biochemistry 2023Quote: ... and assembled using T4-ligase and BsaI-HF-v2 using cycles of 16°C and 37°C (New-England Biolabs). The resulting assembly is PCR amplified to add the final gates and assembled into a pBAD vector with appropriate gates.
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bioRxiv - Molecular Biology 2022Quote: ... containing the ABCA4 minigene genomic region and the c.5461-10T>C mutation were digested using the EcoRI (New England Biolabs) and SalI (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... we digested #126-pSC-T7A1reverse-parS with SpeI-HF and BamHI-HF 1 h at 37°C and heat-inactivated for 20 min at 80°C (New England Biolabs). Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega ...
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bioRxiv - Biophysics 2023Quote: ... The 38 kbp T7A1-parS + handle construct were subsequently digested for 1 h at 37°C and heat-inactivated for 20 min at 65°C using SrfI restriction enzyme (New England Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... Second-strand DNA synthesis was done by ligating 10 µM of a blunt-end duplex comprised of a 32-bp Illumina R1-3’SpC3 and 5’-phosphorylated R1R-3’SpC3 DNA (pre-annealed by incubating at 95°C for 3 min and slowly cooling to 25°C) using a Quick ligase kit (New England Biolabs) according to manufacturer’s protocol followed by clean-up as described above ...
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bioRxiv - Cell Biology 2023Quote: ... A de novo sequence was formed by annealing oligo 3 and 4 over a 30 min temperature gradient from 95°C to 25°C and integrated via T4 ligase (New England Biolabs). pZE13d-C_PfCen3 and _PfCen4 were generated by digesting pZE13d-C with BamHI-HF and SalI-HF and integrating codon optimized PfCen3 and PfCen4 encoding sequences PCR amplified via primer pairs P34+35 and P36+37 ...
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bioRxiv - Genomics 2023Quote: ... 30 minutes at 72°C and finally held at 4°C until 1 μl Exonuclease I (20U/μl, catalog num. M0293S, NEB) was added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... the remaining 5’-phosphate groups were dephosphorylated using calf-intestinal alkaline phosphatase for 10 minutes at 37°C and finally heat inactivated for 20 min at 80°C (Quick CIP, New England Biolabs). The Rap1 arrays initially cloned in a pUC19-derived vector (Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cosmids were then digested for 2 hours at 37°C and heat-inactivated for 20 minutes at 80°C using SpeI-HF (New England Biolabs). Next ...
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bioRxiv - Immunology 2021Quote: ... coli cells (NEB, catalog no C3040H, 30°C). To generate gp70 C.1086 V1V2 RQ(H/Y)173 constructs ...
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bioRxiv - Evolutionary Biology 2022Quote: ... overnight DpnI digestion at 37°C (NEB #R0176), and a second 2X ratio bead cleanup ...
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bioRxiv - Microbiology 2023Quote: ... at 37 °C or Bt.NstNBI nicking endonuclease (NEB) at 55 °C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... and Proteinase K (NEB, 60°C, 1 hour). Samples were combined with SDS-PAGE loading buffer ...
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bioRxiv - Genetics 2023Quote: ... We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles, Tm 65 °C). We then performed a bead cleanup ...
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CRISpy-pop: a web tool for designing CRISPR/Cas9-driven genetic modifications in diverse populationsbioRxiv - Genetics 2020Quote: The 3’ and 5’ sections of the donor DNA were first amplified individually using gradient PCR with annealing temperatures from 50°C – 70°C and Phusion® High-Fidelity DNA Polymerase (New England Biolabs). The two individual sections were then joined into the complete donor DNA fragment using the same gradient PCR protocol ...
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bioRxiv - Microbiology 2022Quote: ... the CPER product was subject to post-PCR nick sealing for 30 min at 50°C and 30 min at 60°C in a 25 μL reaction containing 1 mM β-nicotinamide adenine dinucleotide (NAD+) (NEB) and 0.5 μL HiFi Taq DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50 ng of material was fragmented for 8 min at 37 °C and A-tailed for 30 min at 65 °C using NEBNext Ultra II FS DNA Library Prep Kit (NEB, E7805S). The fragmented and dA-tailed DNA library was ligated to dsDNA adapter having both forward (Ligation FWD primer ...
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bioRxiv - Cell Biology 2024Quote: ... were assembled in 3 separate reactions for 15 minutes at 50 °C or 55 °C using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs E2621S) alongside a control reaction omitting the insert ...